FastQCFastQC Report
Wed 25 May 2016
SRR1779729_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779729_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences138658
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT1446810.434305990278238No Hit
TCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG16851.2152201820306077No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC10320.7442772865611793No Hit
ATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTCTT7280.5250328145509094TruSeq Adapter, Index 2 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC5400.38944741738666355No Hit
CTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTC4170.30073995009303467No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTACCTCGTATGCCGT3050.21996567093135627No Hit
CTTTACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTC2710.1954449076144182No Hit
CTTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG1660.11971902090034472No Hit
TTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTC1510.10890103708404851No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC1430.1031314457153572No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTGT207.904091E-570.034
GTACACT150.002220769270.036
CAGCATG150.002220769270.09
CTATAAG150.002220769270.038
TTAAGCC150.002220769270.031
CATCGTT207.904091E-570.032
CGTAAGA150.002220769270.032
CACTTAC150.002220769270.039
TCTTTAT352.9567855E-760.0000041
TACGACA252.3836856E-455.99999649
ACAACGA200.00693921552.570
GTCTGGA200.00693921552.558
CTTCAAG200.00693921552.544
GGACTTG200.00693921552.518
ACTCTGG200.00693921552.58
GAGAGGC200.00693921552.533
CGTAGCC200.00693921552.530
TAACAAG407.4362833E-752.531
TCGTAAG200.00693921552.531
GACTGCA200.00693921552.57
Produced by FastQC (version 0.11.5)