##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779729_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	138658
Sequences flagged as poor quality	0
Sequence length	76
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.65691124926077	34.0	34.0	34.0	34.0	34.0
2	33.65899551414271	34.0	34.0	34.0	34.0	34.0
3	33.69500497627256	34.0	34.0	34.0	34.0	34.0
4	33.71951131561107	34.0	34.0	34.0	34.0	34.0
5	33.71673469976489	34.0	34.0	34.0	34.0	34.0
6	37.37598984551919	38.0	38.0	38.0	37.0	38.0
7	37.375304706544156	38.0	38.0	38.0	37.0	38.0
8	37.26826436267651	38.0	38.0	38.0	36.0	38.0
9	37.25699202353993	38.0	38.0	38.0	36.0	38.0
10-11	37.178136133508346	38.0	38.0	38.0	36.0	38.0
12-13	37.14753566328665	38.0	38.0	38.0	36.0	38.0
14-15	37.180580997850825	38.0	38.0	38.0	36.0	38.0
16-17	37.16112665695452	38.0	38.0	38.0	36.0	38.0
18-19	37.15851952285479	38.0	38.0	38.0	36.0	38.0
20-21	37.15168976907211	38.0	38.0	38.0	36.0	38.0
22-23	37.12783972075178	38.0	38.0	38.0	36.0	38.0
24-25	37.1120562823638	38.0	38.0	38.0	36.0	38.0
26-27	37.045197536384485	38.0	38.0	38.0	35.5	38.0
28-29	36.980668262920275	38.0	38.0	38.0	35.5	38.0
30-31	36.803274964300655	38.0	37.0	38.0	35.0	38.0
32-33	36.83991186949184	38.0	37.5	38.0	35.0	38.0
34-35	36.87329256155433	38.0	38.0	38.0	35.0	38.0
36-37	36.8492441835307	38.0	37.0	38.0	35.0	38.0
38-39	36.361760590806156	38.0	37.0	38.0	34.0	38.0
40-41	36.78210056397756	38.0	37.0	38.0	34.5	38.0
42-43	36.95099092731758	38.0	37.5	38.0	35.0	38.0
44-45	36.94391957189632	38.0	38.0	38.0	35.0	38.0
46-47	36.96585483708117	38.0	38.0	38.0	35.5	38.0
48-49	36.94972882920567	38.0	38.0	38.0	35.5	38.0
50-51	36.87715097578214	38.0	37.5	38.0	35.0	38.0
52-53	36.868983398000836	38.0	38.0	38.0	35.0	38.0
54-55	36.85551861414415	38.0	37.5	38.0	35.0	38.0
56-57	36.88204431046171	38.0	37.0	38.0	35.0	38.0
58-59	36.81178150557487	38.0	37.0	38.0	35.0	38.0
60-61	36.88161880309828	38.0	37.5	38.0	35.0	38.0
62-63	36.91249693490458	38.0	38.0	38.0	35.0	38.0
64-65	36.93589623389923	38.0	38.0	38.0	35.0	38.0
66-67	36.875917725627076	38.0	38.0	38.0	35.0	38.0
68-69	36.70630255737137	38.0	37.5	38.0	34.5	38.0
70-71	35.76475933592003	38.0	37.0	38.0	29.5	38.0
72-73	34.171977094722266	38.0	36.5	38.0	21.0	38.0
74-75	33.98321409511171	38.0	36.0	38.0	19.0	38.0
76	33.556779991057134	38.0	34.0	38.0	19.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	2.0
21	3.0
22	6.0
23	16.0
24	36.0
25	66.0
26	131.0
27	227.0
28	472.0
29	715.0
30	1223.0
31	1872.0
32	2955.0
33	4483.0
34	8117.0
35	15841.0
36	21068.0
37	81425.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.13885964026598	16.461365373797403	10.992513955199124	35.407261030737494
2	19.626707438445674	16.965483419636804	39.3197651776313	24.08804396428623
3	17.823710135729637	19.165861327871454	36.70686148653522	26.30356704986369
4	13.171977094722267	25.157581964257382	32.95879069364912	28.711650247371228
5	12.952732622711997	31.127666633010715	41.001601061604816	14.917999682672475
6	26.96057926697342	41.57351180602634	16.653925485727473	14.811983441272773
7	26.04105064258824	26.51992672618962	18.783625899695654	28.65539673152649
8	22.81657026641088	40.240736199858645	16.59911436772491	20.34357916600557
9	21.483794660243188	16.405833056873746	17.082317644852804	45.02805463803026
10-11	22.89193555366441	27.297379163120773	27.717477534653607	22.09320774856121
12-13	22.694687648747276	19.368157625236194	28.31030304778664	29.626851678229887
14-15	20.749974758037762	19.907975017669376	20.757907946169713	38.58414227812315
16-17	27.857750724804912	27.906792251438794	20.492867342670458	23.74258968108584
18-19	27.74812848879978	23.314197521960505	21.148437161938006	27.789236827301707
20-21	21.11273781534423	22.14729766764269	21.25048680927173	35.48947770774135
22-23	21.439801526056918	28.495651170505848	21.348209263078942	28.71633804035829
24-25	27.45099453331218	28.723550029569157	21.126079995384327	22.69937544173434
26-27	27.666633010717018	29.527686826580506	21.661930793751534	21.143749368950946
28-29	27.543668594671782	23.57995932438085	21.92228360426373	26.95408847668364
30-31	27.764716063984768	28.364032367407578	21.666979186199136	22.204272382408515
32-33	21.654718804540668	28.38098054205311	22.062196194954492	27.90210445845173
34-35	26.709962641895892	23.170318337203767	22.580377619755083	27.53934140114526
36-37	22.214008567843184	27.128979215047096	28.540726103073748	22.116286114035972
38-39	20.16219763735233	23.11190122459577	29.62000028847957	27.10590084957233
40-41	20.061590387860782	21.08821705202729	30.513205152245092	28.336987407866836
42-43	26.510551140215494	20.85779399674018	29.857635332977544	22.774019530066784
44-45	20.156788645444188	27.00709659738349	30.76923076923077	22.066883987941555
46-47	25.896450258910413	21.118868006173464	25.05661411530528	27.928067619610843
48-49	25.248453028314273	20.378917913138803	24.618485770745288	29.754143287801643
50-51	20.03779082346493	20.759350344011885	30.628236380158373	28.57462245236481
52-53	18.341530961069683	20.493588541591542	37.41796362272642	23.746916874612356
54-55	17.945953352853785	20.9814796117065	32.11715155274128	28.955415482698438
56-57	24.270867890781638	21.004197377720722	25.750046877929872	28.974887853567772
58-59	18.999624976561037	21.47153427858472	35.50318048724199	24.025660257612255
60-61	24.295028054638028	27.73839230336511	24.77174054147615	23.194839100520706
62-63	17.929365777668796	35.79598724920308	22.61283157120397	23.66181540192416
64-65	17.494482828253688	36.998947049575214	21.645343218566545	23.861226903604553
66-67	17.63511661786554	36.893291407636056	21.113819613725855	24.357772360772547
68-69	17.77575040747739	37.14715342785847	20.73843557530038	24.33866058936376
70-71	18.43672921865309	35.963665998355665	20.801901080355982	24.79770370263526
72-73	19.261420184915405	32.31295705981624	21.39075999942304	27.034862755845317
74-75	19.725872290095054	30.99208123584647	21.703760331174546	27.57828614288393
76	19.849557905061374	31.07285551500815	21.67274877756783	27.404837802362646
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.5
14	0.5
15	1.5
16	1.5
17	0.0
18	5.0
19	12.0
20	14.0
21	14.0
22	29.5
23	56.5
24	126.0
25	184.0
26	230.0
27	277.0
28	364.0
29	450.0
30	608.0
31	866.5
32	1250.5
33	1534.0
34	1773.0
35	2352.5
36	2951.0
37	3209.0
38	3601.5
39	4586.0
40	5755.0
41	9250.0
42	12168.0
43	12875.0
44	12839.0
45	11016.0
46	9936.0
47	9199.0
48	7805.0
49	6573.0
50	5998.0
51	5633.5
52	4666.5
53	3735.5
54	3407.0
55	3055.5
56	2511.0
57	2056.5
58	1795.0
59	1668.5
60	1547.0
61	1483.5
62	1415.0
63	1418.0
64	1436.0
65	1491.5
66	1457.0
67	1382.0
68	1350.5
69	1322.5
70	1332.5
71	1339.0
72	1339.5
73	1298.5
74	1183.5
75	1110.0
76	1061.5
77	888.0
78	701.5
79	640.0
80	558.0
81	392.5
82	268.0
83	227.0
84	178.0
85	102.5
86	54.5
87	33.0
88	27.0
89	13.0
90	2.5
91	0.5
92	1.0
93	1.5
94	1.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	138658.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.848144355176046
#Duplication Level	Percentage of deduplicated	Percentage of total
1	47.04483695652174	14.982907585570251
2	22.384510869565215	14.258102669878406
3	12.033514492753623	11.497353199959614
4	6.107336956521739	7.780293960680234
5	3.308423913043478	5.268358118536255
6	1.9859601449275361	3.7949487227567107
7	1.589673913043478	3.5439714982186383
8	1.0235507246376812	2.6078552986484733
9	0.7608695652173914	2.1809055373653163
>10	3.6956521739130435	18.751171948246764
>50	0.02943840579710145	0.5430627875780697
>100	0.024909420289855072	1.4827849817536674
>500	0.004528985507246377	0.914480231937573
>1k	0.004528985507246377	1.959497468591787
>5k	0.0	0.0
>10k+	0.0022644927536231885	10.434305990278238
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT	14468	10.434305990278238	No Hit
TCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG	1685	1.2152201820306077	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC	1032	0.7442772865611793	No Hit
ATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTCTT	728	0.5250328145509094	TruSeq Adapter, Index 2 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC	540	0.38944741738666355	No Hit
CTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTC	417	0.30073995009303467	No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTACCTCGTATGCCGT	305	0.21996567093135627	No Hit
CTTTACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTC	271	0.1954449076144182	No Hit
CTTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG	166	0.11971902090034472	No Hit
TTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTC	151	0.10890103708404851	No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC	143	0.1031314457153572	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	7.211989210864141E-4	0.0
6	0.0	0.0	0.0	7.211989210864141E-4	0.0
7	0.0	0.0	0.0	0.0014423978421728282	0.0
8	0.0	0.0	0.0	0.0028847956843456564	0.0
9	0.0	0.0	0.0	0.005769591368691313	0.0
10	0.0	0.0	0.0	0.007933188131950555	0.0
11	0.0	0.0	0.0	0.010096784895209796	0.0
12	0.0	0.0	0.0	0.011539182737382626	0.0
13	0.0	0.0	0.0	0.011539182737382626	0.0
14	0.0	0.0	0.0	0.011539182737382626	0.0
15	0.0	0.0	0.0	0.013702779500641867	0.0
16	0.0	0.0	0.0	0.01586637626390111	0.0
17	0.0	0.0	0.0	0.01586637626390111	0.0
18	0.0	0.0	0.0	0.018751171948246766	0.0
19	0.0	0.0	0.0	0.020193569790419592	0.0
20	0.0	0.0	0.0	0.021635967632592422	0.0
21	0.0	0.0	0.0	0.025241962238024492	0.0
22	0.0	0.0	0.0	0.028126757922370148	0.0
23	0.0	0.0	0.0	0.03389634929106146	0.0
24	0.0	0.0	0.0	0.0411083385019256	0.0
25	0.0	0.0	0.0	0.04543553202844409	0.0
26	0.0	0.0	0.0	0.050483924476048984	0.0
27	0.0	0.0	0.0	0.08293787592493762	0.0
28	0.0	0.0	0.0	0.12044021982143115	0.0
29	0.0	0.0	0.0	0.16731814969204806	0.0
30	0.0	0.0	0.0	0.21419607956266498	0.0
31	0.0	0.0	0.0	0.2805463803026151	0.0
32	0.0	0.0	0.0	0.3548298691745157	0.0
33	0.0	0.0	0.0	0.45002812675792236	0.0
34	0.0	0.0	0.0	0.6022010991071557	0.0
35	0.0	0.0	0.0	0.7788948347733272	0.0
36	0.0	0.0	0.0	1.0529504247861645	0.0
37	0.0	0.0	0.0	1.480621384990408	0.0
38	0.0	0.0	0.0	2.030896161779342	0.0
39	0.0	0.0	0.0	2.6619452177299543	0.0
40	0.0	0.0	0.0	3.2756854995744926	0.0
41	0.0	0.0	0.0	4.058186328953252	0.0
42	0.0	0.0	0.0	4.84501435185853	0.0
43	0.0	0.0	0.0	5.665738724054869	0.0
44	0.0	0.0	0.0	6.526129036910961	0.0
45	0.0	0.0	0.0	7.333871828527744	0.0
46	0.0	0.0	0.0	8.18128056080428	0.0
47	0.0	0.0	0.0	9.053931255318842	0.0
48	0.0	0.0	0.0	9.924418353070143	0.0
49	0.0	0.0	0.0	10.740094332818877	0.0
50	0.0	0.0	0.0	11.555770312567612	0.0
51	0.0	0.0	0.0	12.318077572155952	0.0
52	0.0	0.0	0.0	13.152504723852934	0.0
53	0.0	0.0	0.0	13.942217542442556	0.0
54	0.0	0.0	0.0	14.744911941611734	0.0
55	0.0	0.0	0.0	15.510103996884421	0.0
56	0.0	0.0	0.0	16.21399414386476	0.0
57	0.0	0.0	0.0	16.949617043372903	0.0
58	0.0	0.0	0.0	17.713366700803416	0.0
59	0.0	0.0	0.0	18.421584041310275	0.0
60	0.0	0.0	0.0	19.13268617750148	0.0
61	0.0	0.0	0.0	19.851000302903547	0.0
62	0.0	0.0	0.0	20.558496444489318	0.0
63	0.0	0.0	0.0	21.228490242178598	0.0
64	0.0	0.0	0.0	21.970603931976516	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTGT	20	7.904091E-5	70.0	34
GTACACT	15	0.0022207692	70.0	36
CAGCATG	15	0.0022207692	70.0	9
CTATAAG	15	0.0022207692	70.0	38
TTAAGCC	15	0.0022207692	70.0	31
CATCGTT	20	7.904091E-5	70.0	32
CGTAAGA	15	0.0022207692	70.0	32
CACTTAC	15	0.0022207692	70.0	39
TCTTTAT	35	2.9567855E-7	60.000004	1
TACGACA	25	2.3836856E-4	55.999996	49
ACAACGA	20	0.006939215	52.5	70
GTCTGGA	20	0.006939215	52.5	58
CTTCAAG	20	0.006939215	52.5	44
GGACTTG	20	0.006939215	52.5	18
ACTCTGG	20	0.006939215	52.5	8
GAGAGGC	20	0.006939215	52.5	33
CGTAGCC	20	0.006939215	52.5	30
TAACAAG	40	7.4362833E-7	52.5	31
TCGTAAG	20	0.006939215	52.5	31
GACTGCA	20	0.006939215	52.5	7
>>END_MODULE