FastQCFastQC Report
Wed 25 May 2016
SRR1779728_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779728_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69053
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCGTA150.00221603570.034
CGATTGT150.00221603570.046
GTGTACA252.812796E-670.036
GTATCCT150.00221603570.057
GCTAGAA150.00221603570.028
GCCTACG252.812796E-670.020
TAGCTCT207.876043E-570.039
AGATCTC1000.059.49999640
TCTCGGT1000.059.49999643
AGCTCTC308.297326E-658.33333240
TCATTCA252.375278E-455.99999660
TCGTGTA252.375278E-455.99999634
TCCCGAC252.375278E-455.99999619
AGCTAGA252.375278E-455.99999627
TAGATCT950.055.26315739
GCGTGTA452.6540874E-854.44444734
TTTAGAT200.006924534752.537
TTAGATC200.006924534752.538
GTCTAGA200.006924534752.536
TTCCGAC407.381459E-752.519
Produced by FastQC (version 0.11.5)