FastQCFastQC Report
Wed 25 May 2016
SRR1779728_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779728_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69053
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT1524422.075796851693628No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG18272.6457938105513157No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC11221.6248388918656684No Hit
ATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTCTT7741.1208781660463703RNA PCR Primer, Index 47 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC4700.6806366124570982No Hit
CTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTC4280.6198137662375277No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGACCTCGTATGCCGT3280.47499746571474083No Hit
CTTTACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTC2990.43300073856313265No Hit
TTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTC1860.26935831897238355No Hit
CTTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG1490.21577628777895239No Hit
CTTAATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG1410.20419098373712943No Hit
TATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTCT1350.19550200570576226No Hit
TCTTAATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC1260.18246853865871143No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGAGCTCGTATGCCGT1250.18102037565348356No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATGGTAGACCTCGTATGCCG1180.17088323461688848No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGAACTCGTATGCCGT1170.16943507161166063No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCCAA150.00221603570.011
TAGAATG150.00221603570.04
ATCGTAT150.00221603570.039
GGAAGTC150.00221603570.028
ATATCGT150.00221603570.037
ACAGCCA150.00221603570.062
CTATTGT150.00221603570.01
CTCGAAA150.00221603570.057
TCTTTAT550.070.01
TTGCCAC150.00221603570.030
TATCGTA150.00221603570.038
TTCTGGG150.00221603570.02
TCGAAAA150.00221603570.058
GTTGCCA150.00221603570.029
CTATACA352.9348848E-760.01
CTTTATA650.059.2307662
AACTCGT252.375278E-455.99999637
CTTTACA252.375278E-455.9999961
TTTATAC700.055.03
GTAGACC653.6379788E-1253.84615333
Produced by FastQC (version 0.11.5)