##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779728_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	69053
Sequences flagged as poor quality	0
Sequence length	76
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.63785787728267	34.0	34.0	34.0	34.0	34.0
2	33.63739446512099	34.0	34.0	34.0	34.0	34.0
3	33.65039896890794	34.0	34.0	34.0	34.0	34.0
4	33.688369802905015	34.0	34.0	34.0	34.0	34.0
5	33.68852910083559	34.0	34.0	34.0	34.0	34.0
6	37.31920408961233	38.0	38.0	38.0	36.0	38.0
7	37.318002114317984	38.0	38.0	38.0	36.0	38.0
8	37.212358623086615	38.0	38.0	38.0	36.0	38.0
9	37.25480428076985	38.0	38.0	38.0	36.0	38.0
10-11	37.137633412016854	38.0	38.0	38.0	36.0	38.0
12-13	37.11672193822136	38.0	38.0	38.0	36.0	38.0
14-15	37.140812129813334	38.0	38.0	38.0	36.0	38.0
16-17	37.055754275701275	38.0	38.0	38.0	35.5	38.0
18-19	37.05508087990384	38.0	38.0	38.0	35.0	38.0
20-21	37.09484019521237	38.0	38.0	38.0	35.5	38.0
22-23	37.04794143628807	38.0	38.0	38.0	35.0	38.0
24-25	37.01773999681404	38.0	38.0	38.0	35.0	38.0
26-27	36.92123441414566	38.0	38.0	38.0	35.0	38.0
28-29	36.769184539411754	38.0	37.5	38.0	34.5	38.0
30-31	36.54435723285013	38.0	37.0	38.0	34.0	38.0
32-33	36.62250010861223	38.0	37.0	38.0	34.0	38.0
34-35	36.63824164047905	38.0	37.0	38.0	34.0	38.0
36-37	36.60779401329414	38.0	37.0	38.0	34.0	38.0
38-39	35.31829174691903	38.0	36.0	38.0	29.0	38.0
40-41	36.34114375914153	38.0	37.0	38.0	34.0	38.0
42-43	36.78177631674221	38.0	37.0	38.0	34.5	38.0
44-45	36.77696117474983	38.0	37.0	38.0	34.5	38.0
46-47	36.8090307445006	38.0	37.0	38.0	34.5	38.0
48-49	36.785548781370835	38.0	37.0	38.0	34.5	38.0
50-51	36.65246984200542	38.0	37.0	38.0	34.0	38.0
52-53	36.6467858022099	38.0	37.0	38.0	34.0	38.0
54-55	36.64908114057319	38.0	37.0	38.0	34.0	38.0
56-57	36.671983838500864	38.0	37.0	38.0	34.0	38.0
58-59	36.520187392292875	38.0	37.0	38.0	34.0	38.0
60-61	36.58251632803788	38.0	37.0	38.0	34.0	38.0
62-63	36.62331107989515	38.0	37.0	38.0	34.0	38.0
64-65	36.646011035002104	38.0	37.0	38.0	34.0	38.0
66-67	36.5357623854141	38.0	37.0	38.0	34.0	38.0
68-69	36.18798603970863	38.0	37.0	38.0	33.5	38.0
70-71	34.134700881931266	38.0	34.0	38.0	21.0	38.0
72-73	30.7187812260148	38.0	23.0	38.0	11.0	38.0
74-75	30.281674945331847	38.0	23.0	38.0	11.0	38.0
76	29.93962608431205	37.0	23.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	1.0
21	2.0
22	9.0
23	17.0
24	27.0
25	42.0
26	82.0
27	172.0
28	327.0
29	566.0
30	849.0
31	1409.0
32	2030.0
33	3189.0
34	5605.0
35	12333.0
36	12470.0
37	29922.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.226738881728526	11.721431364314368	10.697580119618264	46.35424963433884
2	16.553951312759764	11.557788944723619	47.82847957366081	24.059780168855806
3	17.262103022316193	13.107323360317439	44.14724921437157	25.4833244029948
4	13.693829377434724	33.112247114535215	24.34362011788047	28.850303390149595
5	14.746643882235386	24.930126134997753	45.055247418649444	15.267982564117416
6	27.44848160108902	44.003881076854015	12.545436114289025	16.002201207767946
7	24.255282174561568	20.432131840759997	13.286895572965692	42.02569041171274
8	20.398824091639757	45.604101197630804	11.967619075203105	22.029455635526336
9	18.31781385312731	13.6561771392988	10.79605520397376	57.22995380360013
10-11	23.16409135012237	27.472376290675278	25.817850057202442	23.54568230199991
12-13	21.651485091161863	12.120400272254644	28.557774463093565	37.67034017348993
14-15	21.216312108090886	11.87131623535545	14.645272471869433	52.26709918468423
16-17	35.84275846089236	25.38702156314715	11.84886970877442	26.92135026718607
18-19	36.506017117286724	15.919655916469958	11.903175821470464	35.67115114477286
20-21	22.64709715725602	13.46501962260872	12.084920278626562	51.8029629415087
22-23	22.96134852939047	26.953209853301086	11.943724385616845	38.141717231691594
24-25	35.97164496835764	26.933659652730512	11.812665633643723	25.282029745268126
26-27	36.00278047297004	29.774231387484974	12.229736579149348	21.993251560395638
28-29	22.888940379129075	29.21741271197486	13.176111103065761	34.7175358058303
30-31	22.844771407469626	29.037840499326606	25.50215052206276	22.61523757114101
32-33	47.762588156922945	14.735782659696175	16.73714393292109	20.764485250459792
34-35	26.49052177313078	25.79467944911879	27.17260654859311	20.542192229157312
36-37	34.594441950385935	27.9256513113116	15.46348456982318	22.016422168479284
38-39	21.973701359825064	15.59599148480153	27.887999073175678	34.54230808219773
40-41	20.545812636670384	12.573675292890968	30.01607460935803	36.86443746108062
42-43	34.37070076607823	12.259423920756522	28.9335727629502	24.436302550215053
44-45	20.860064008804834	25.83595209476779	31.010962593949575	22.293021302477804
46-47	33.081111609922814	13.798097113811131	19.207710019839833	33.913081256426224
48-49	32.512707630370876	12.642463035639292	16.165119545856083	38.679709788133756
50-51	21.008500716840686	13.08560091523902	30.118170101226593	35.7877282666937
52-53	17.9485322867942	13.092841730265159	43.8112753971587	25.147350585781936
54-55	18.128828580945072	12.826379737303231	33.07314671339406	35.97164496835764
56-57	31.720562466511232	12.940784614716232	19.83621276410873	35.50244015466381
58-59	19.957858456547868	14.575036566115882	40.75782370063574	24.709281276700505
60-61	31.722010629516458	28.062502715305637	18.376464454839038	21.83902220033887
62-63	18.689267663968256	45.33981144917672	13.64531591675959	22.325604970095434
64-65	17.580698883466322	47.25717926809841	13.124701316380172	22.037420532055087
66-67	18.110726543379723	47.3809972050454	12.40496430278192	22.103311948792957
68-69	17.739272732538776	47.88640609386993	12.725732408439894	21.648588765151406
70-71	17.943463716275904	45.04293803310501	13.724964882047122	23.28863336857197
72-73	19.097649631442515	37.60444875675206	16.59377579540353	26.7041258164019
74-75	20.17870331484512	34.261364459183525	18.06583349021766	27.494098735753695
76	20.094709860541904	34.8992802629864	17.761719259119808	27.24429061735189
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.5
15	1.0
16	3.5
17	6.0
18	7.0
19	6.5
20	8.0
21	11.0
22	23.0
23	30.5
24	38.5
25	51.0
26	59.5
27	84.5
28	121.5
29	142.0
30	144.0
31	170.0
32	237.5
33	281.0
34	336.0
35	407.0
36	426.0
37	429.0
38	660.0
39	3890.0
40	7397.0
41	6976.0
42	6047.0
43	4999.0
44	3107.0
45	1839.0
46	1415.0
47	1279.5
48	1032.0
49	903.0
50	886.0
51	821.5
52	704.5
53	745.0
54	838.0
55	864.5
56	953.0
57	1103.0
58	1191.0
59	1270.0
60	1458.0
61	1666.5
62	1766.0
63	1769.0
64	1829.0
65	1884.0
66	1936.5
67	1991.0
68	1999.5
69	2091.5
70	2123.5
71	2072.0
72	1932.5
73	1790.5
74	1687.0
75	1586.0
76	1516.5
77	1283.0
78	1035.5
79	952.0
80	829.0
81	575.0
82	397.0
83	350.0
84	273.0
85	167.5
86	94.0
87	49.0
88	37.0
89	18.5
90	8.5
91	2.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	69053.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.968618307676714
#Duplication Level	Percentage of deduplicated	Percentage of total
1	57.10728752462002	22.253920901336656
2	24.757516072689434	19.295323881656117
3	10.843955553903898	12.677218947764759
4	4.214203426362927	6.568867391713611
5	1.5719647701512505	3.0628647560569418
6	0.6800698651008956	1.5900829797401996
7	0.26013601397302016	0.7095998725616556
8	0.1486491508417258	0.4634121616729179
9	0.09662194804712178	0.33887014322332126
>10	0.2527035564309339	1.597323794766339
>50	0.007432457542086291	0.1781240496430278
>100	0.04459474525251775	3.797083399707471
>500	0.0037162287710431454	1.1208781660463703
>1k	0.007432457542086291	4.270632702416984
>5k	0.0	0.0
>10k+	0.0037162287710431454	22.075796851693628
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT	15244	22.075796851693628	No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG	1827	2.6457938105513157	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC	1122	1.6248388918656684	No Hit
ATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTCTT	774	1.1208781660463703	RNA PCR Primer, Index 47 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC	470	0.6806366124570982	No Hit
CTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTC	428	0.6198137662375277	No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGACCTCGTATGCCGT	328	0.47499746571474083	No Hit
CTTTACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTC	299	0.43300073856313265	No Hit
TTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTC	186	0.26935831897238355	No Hit
CTTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG	149	0.21577628777895239	No Hit
CTTAATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG	141	0.20419098373712943	No Hit
TATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTCT	135	0.19550200570576226	No Hit
TCTTAATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC	126	0.18246853865871143	No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGAGCTCGTATGCCGT	125	0.18102037565348356	No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATGGTAGACCTCGTATGCCG	118	0.17088323461688848	No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGAACTCGTATGCCGT	117	0.16943507161166063	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.01882611906796229	0.0
2	0.0	0.0	0.0	0.01882611906796229	0.0
3	0.0	0.0	0.0	0.01882611906796229	0.0
4	0.0	0.0	0.0	0.024618771088873764	0.0
5	0.0	0.0	0.0	0.030411423109785236	0.0
6	0.0	0.0	0.0	0.030411423109785236	0.0
7	0.0	0.0	0.0	0.030411423109785236	0.0
8	0.0	0.0	0.0	0.030411423109785236	0.0
9	0.0	0.0	0.0	0.03910040114115245	0.0
10	0.0	0.0	0.0	0.04199672715160818	0.0
11	0.0	0.0	0.0	0.04199672715160818	0.0
12	0.0	0.0	0.0	0.04489305316206392	0.0
13	0.0	0.0	0.0	0.04489305316206392	0.0
14	0.0	0.0	0.0	0.04778937917251966	0.0
15	0.0	0.0	0.0	0.050685705182975396	0.0
16	0.0	0.0	0.0	0.052133868188203265	0.0
17	0.0	0.0	0.0	0.055030194198659	0.0
18	0.0	0.0	0.0	0.055030194198659	0.0
19	0.0	0.0	0.0	0.06227100922479834	0.0
20	0.0	0.0	0.0	0.06371917223002621	0.0
21	0.0	0.0	0.0	0.07240815026139343	0.0
22	0.0	0.0	0.0	0.08109712829276064	0.0
23	0.0	0.0	0.0	0.0868897803136721	0.0
24	0.0	0.0	0.0	0.09123426932935572	0.0
25	0.0	0.0	0.0	0.09413059533981145	0.0
26	0.0	0.0	0.0	0.09992324736072292	0.0
27	0.0	0.0	0.0	0.15495344155938193	0.0
28	0.0	0.0	0.0	0.23315424384168681	0.0
29	0.0	0.0	0.0	0.3229403501658147	0.0
30	0.0	0.0	0.0	0.40114115244811954	0.0
31	0.0	0.0	0.0	0.5097533778402097	0.0
32	0.0	0.0	0.0	0.6227100922479835	0.0
33	0.0	0.0	0.0	0.7747672077969097	0.0
34	0.0	0.0	0.0	1.045573689774521	0.0
35	0.0	0.0	0.0	1.3989254630501209	0.0
36	0.0	0.0	0.0	1.8608894617178109	0.0
37	0.0	0.0	0.0	2.621175039462442	0.0
38	0.0	0.0	0.0	3.378564291196617	0.0
39	0.0	0.0	0.0	4.0548564146380315	0.0
40	0.0	0.0	0.0	4.699288951964433	0.0
41	0.0	0.0	0.0	5.371236586390164	0.0
42	0.0	0.0	0.0	6.035943405789756	0.0
43	0.0	0.0	0.0	6.700650225189348	0.0
44	0.0	0.0	0.0	7.297293383343229	0.0
45	0.0	0.0	0.0	8.090886710208101	0.0
46	0.0	0.0	0.0	8.80483107178544	0.0
47	0.0	0.0	0.0	9.521671759373236	0.0
48	0.0	0.0	0.0	10.111074102500977	0.0
49	0.0	0.0	0.0	10.642549925419605	0.0
50	0.0	0.0	0.0	11.13492534719708	0.0
51	0.0	0.0	0.0	11.647575051047745	0.0
52	0.0	0.0	0.0	12.180499036971602	0.0
53	0.0	0.0	0.0	12.619292427555646	0.0
54	0.0	0.0	0.0	13.108771523322666	0.0
55	0.0	0.0	0.0	13.556253891938077	0.0
56	0.0	0.0	0.0	13.96173953340188	0.0
57	0.0	0.0	0.0	14.445425977147988	0.0
58	0.0	0.0	0.0	14.884219367732031	0.0
59	0.0	0.0	0.0	15.25205277105991	0.0
60	0.0	0.0	0.0	15.595267403298916	0.0
61	0.0	0.0	0.0	16.010890185799315	0.0
62	0.0	0.0	0.0	16.403342360216065	0.0
63	0.0	0.0	0.0	16.826205957742605	0.0
64	0.0	0.0	0.0	17.29106628242075	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCCAA	15	0.002216035	70.0	11
TAGAATG	15	0.002216035	70.0	4
ATCGTAT	15	0.002216035	70.0	39
GGAAGTC	15	0.002216035	70.0	28
ATATCGT	15	0.002216035	70.0	37
ACAGCCA	15	0.002216035	70.0	62
CTATTGT	15	0.002216035	70.0	1
CTCGAAA	15	0.002216035	70.0	57
TCTTTAT	55	0.0	70.0	1
TTGCCAC	15	0.002216035	70.0	30
TATCGTA	15	0.002216035	70.0	38
TTCTGGG	15	0.002216035	70.0	2
TCGAAAA	15	0.002216035	70.0	58
GTTGCCA	15	0.002216035	70.0	29
CTATACA	35	2.9348848E-7	60.0	1
CTTTATA	65	0.0	59.230766	2
AACTCGT	25	2.375278E-4	55.999996	37
CTTTACA	25	2.375278E-4	55.999996	1
TTTATAC	70	0.0	55.0	3
GTAGACC	65	3.6379788E-12	53.846153	33
>>END_MODULE