FastQCFastQC Report
Wed 25 May 2016
SRR1779727_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779727_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42799
Sequences flagged as poor quality0
Sequence length76
%GC52

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGGTG950.070.0000144
CTAGAAA150.002210240370.029
TGATACC150.002210240370.02
GGTGTAG252.7944516E-670.035
ATGGCGG207.841763E-570.045
GACGTAA150.002210240370.023
TCTTAGG150.002210240370.045
TTGTCAT207.841763E-570.02
GTCTAGA150.002210240370.036
CACGAGT150.002210240370.033
GCGTTGA150.002210240370.036
AGAGTAG207.841763E-570.035
TCGTGTA401.2914825E-1070.034
GGTGGAA150.002210240370.047
TCTAACG252.7944516E-670.011
ATGACCA150.002210240370.062
GCTAGAA150.002210240370.028
GAGCCAG150.002210240370.048
CGTGTTT150.002210240370.035
GATCTTG207.841763E-570.041
Produced by FastQC (version 0.11.5)