##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779727_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	42799
Sequences flagged as poor quality	0
Sequence length	76
%GC	52
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	21.982663146335195	23.0	12.0	32.0	12.0	34.0
2	22.901890231080166	23.0	12.0	32.0	12.0	34.0
3	22.796303651954485	23.0	11.0	32.0	11.0	34.0
4	21.959788780111683	23.0	12.0	32.0	11.0	34.0
5	22.628753008247855	23.0	11.0	32.0	11.0	34.0
6	22.986401551438117	23.0	11.0	34.0	11.0	37.0
7	23.99913549381995	26.0	12.0	34.0	12.0	38.0
8	23.29545082829038	26.0	11.0	34.0	11.0	38.0
9	24.82543984672539	26.0	12.0	34.0	12.0	38.0
10-11	23.644617864903385	25.5	11.0	34.0	11.0	38.0
12-13	24.33107081941167	26.5	11.0	35.0	11.0	37.5
14-15	22.843816444309446	23.0	11.0	34.0	11.0	37.0
16-17	23.143683263627658	24.5	11.0	33.5	11.0	37.5
18-19	23.571520362625293	25.5	11.5	34.0	11.0	37.0
20-21	22.725262272483	23.0	11.0	33.0	11.0	37.0
22-23	23.00822449122643	24.5	11.0	32.5	11.0	37.5
24-25	22.729900231313813	22.5	11.0	33.5	11.0	37.0
26-27	22.163251477838266	22.5	11.0	32.0	10.0	37.0
28-29	22.45150587630552	22.0	11.0	34.0	10.5	37.5
30-31	22.87650412392813	23.0	11.0	34.0	11.0	38.0
32-33	23.09014229304423	23.0	11.0	34.0	11.0	38.0
34-35	23.14915068109068	23.0	11.0	34.0	11.0	38.0
36-37	22.88568658146218	23.5	11.0	33.0	11.0	37.0
38-39	23.162351924110375	24.5	11.0	33.5	11.0	37.0
40-41	22.05640318699035	22.0	11.0	31.5	11.0	37.0
42-43	22.490992780205147	23.0	11.0	33.0	11.0	37.0
44-45	23.192656370475945	24.0	11.0	34.0	11.0	38.0
46-47	22.916364868338043	23.5	11.0	33.5	11.0	37.5
48-49	22.2058459309797	22.0	11.0	33.0	11.0	37.0
50-51	21.852391411014274	22.5	11.0	31.0	11.0	36.5
52-53	22.152223182784645	22.0	11.0	33.5	11.0	37.0
54-55	22.0433888642258	22.0	11.0	32.5	11.0	37.0
56-57	22.373676955069044	22.5	11.0	32.5	11.0	37.5
58-59	22.435968130096498	22.0	11.0	34.0	11.0	38.0
60-61	23.02729035725134	23.0	11.0	34.0	11.0	38.0
62-63	23.027056706932406	23.0	11.0	34.0	11.0	38.0
64-65	23.09257225636113	23.5	11.0	34.0	11.0	38.0
66-67	22.584744970676887	23.0	11.0	34.0	11.0	37.0
68-69	22.316818149956774	22.5	11.0	34.0	11.0	37.0
70-71	22.34200565433772	23.0	11.0	33.5	11.0	37.0
72-73	22.175763452417115	23.0	11.0	31.0	11.0	37.0
74-75	21.69884810392766	21.0	11.0	32.0	11.0	37.0
76	21.420500478983154	21.0	11.0	31.0	11.0	37.0
>>END_MODULE
>>Per sequence quality scores	fail
#Quality	Count
3	1.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	12.0
12	52.0
13	305.0
14	880.0
15	1463.0
16	2417.0
17	3818.0
18	4899.0
19	5280.0
20	4371.0
21	3182.0
22	2192.0
23	1557.0
24	1193.0
25	912.0
26	688.0
27	618.0
28	636.0
29	595.0
30	626.0
31	669.0
32	788.0
33	1028.0
34	1186.0
35	1416.0
36	1331.0
37	684.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.07764200098133	15.397556017663963	14.705951073623217	46.81885090773149
2	11.872050095798869	11.456142810411702	54.44880601897285	22.22300107481658
3	13.61947709058623	10.446505759480363	53.391434379307924	22.542582770625483
4	12.315708310941845	24.591696067665133	27.43054744269726	35.66204817869576
5	11.467557653216197	17.98406504824879	49.83294002196313	20.715437276571883
6	18.25276291502138	36.56393840977593	16.63356620481787	28.54973247038482
7	17.241459881302866	20.456563390812654	19.17846628347119	43.123510444413284
8	14.580120566381607	34.95023131922053	16.933034253937098	33.53661386046077
9	15.185289032197765	15.12220197205477	17.482125332959484	52.21038366278798
10-11	16.722744053460442	21.950792093088463	30.835553063227255	30.49091079022384
12-13	15.029907939623346	14.580120566381607	36.667601289779896	33.72237020421515
14-15	23.380765456329737	21.58044768447124	23.044301135567082	31.994485723631943
16-17	29.247862049628488	15.491378101780457	21.846815271741672	33.41394457684939
18-19	16.214542735641853	14.674751156596102	32.92910883686154	36.18159727090051
20-21	29.556287676994252	14.33828683583345	23.399457918594326	32.70596756857797
22-23	31.992149165848872	12.791485583438478	21.673909995794197	33.54245525491845
24-25	17.37581195382962	23.260432730501428	20.47408757418571	38.889667741483244
26-27	28.248983597364365	21.88186363848778	21.592130473386607	28.27702229076125
28-29	20.247441469227535	20.728772372540774	24.32823963736623	34.69554652086546
30-31	18.591990279919624	20.6948922846862	26.67297537268097	34.040142062713215
32-33	20.62129071451937	18.70998644796486	26.844712369736907	33.82401046777887
34-35	19.45418010187392	19.810505163792698	26.025748866769476	34.70956586756391
36-37	28.59245759147624	13.324220757979344	32.20360764521706	25.879714005327354
38-39	28.341277629795787	10.548390111687462	38.672367867657364	22.437964390859385
40-41	22.939156035328754	23.83172110846301	24.005794663302023	29.223328192906212
42-43	14.231973456703583	24.912379083134724	29.275900743025375	31.579746717136313
44-45	14.957474648348054	15.54044581522501	34.77732604327305	34.724753493153884
46-47	27.80036450301416	14.576615729706996	26.502406654516562	31.12061311276228
48-49	25.9696714799757	14.8546661058928	32.56226926491892	26.613393149212577
50-51	42.05219870087387	12.306649843450629	23.733585681573903	21.90756577410159
52-53	14.51252993749635	18.86441965068053	32.63274723990887	33.99030317191425
54-55	25.788588251787463	15.782279545773168	25.561942146829292	32.867190055610074
56-57	25.694257842163676	16.720602838950875	24.379928734155033	33.205210584730416
58-59	15.221505677835411	20.50563110425721	30.936025047899435	33.336838170007944
60-61	15.121033693163232	17.423711388382635	30.587877938221414	36.86737698023272
62-63	15.025234824057199	20.916865274078226	29.88691060329922	34.17098929856535
64-65	14.855834384784336	22.768587317164354	29.108836861535586	33.26674143651572
66-67	12.75176410112622	35.43857189588299	18.79410252815552	33.01556147483527
68-69	13.647039577466435	33.089893548650956	19.34002500613468	33.923041867747926
70-71	12.384924529183607	34.819384083368384	17.5522220664517	35.243469320996304
72-73	11.425767559231739	39.65372213654844	16.42483293611851	32.49567736810131
74-75	14.38969110706108	26.98490583672134	21.31524837609234	37.31015468012524
76	13.89317257815786	24.21374830599561	22.877237254077293	39.01584186176924
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.5
6	0.5
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.5
17	1.0
18	1.0
19	3.0
20	7.0
21	9.0
22	11.5
23	14.5
24	21.0
25	27.0
26	41.5
27	66.0
28	100.0
29	124.0
30	165.5
31	241.5
32	309.0
33	342.0
34	411.5
35	574.0
36	758.0
37	849.0
38	907.5
39	1074.0
40	1282.0
41	1462.5
42	1543.0
43	1648.5
44	1856.5
45	2018.5
46	2078.0
47	2055.0
48	2029.5
49	2032.0
50	2037.0
51	2024.5
52	1926.5
53	1764.5
54	1688.0
55	1682.5
56	1618.0
57	1487.0
58	1415.0
59	1391.5
60	1305.0
61	1220.5
62	1199.0
63	1175.0
64	1120.5
65	1018.0
66	935.5
67	925.0
68	873.5
69	751.0
70	678.0
71	676.0
72	611.5
73	492.0
74	433.0
75	429.0
76	363.0
77	272.0
78	185.0
79	123.0
80	129.0
81	101.5
82	57.5
83	47.0
84	40.5
85	24.5
86	9.5
87	4.0
88	4.5
89	2.5
90	1.0
91	1.0
92	0.0
93	1.5
94	1.5
95	0.5
96	1.0
97	0.5
98	1.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0023365031893268534
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0023365031893268534
8	0.0023365031893268534
9	0.0023365031893268534
10-11	0.0023365031893268534
12-13	0.0023365031893268534
14-15	0.0023365031893268534
16-17	0.0023365031893268534
18-19	0.0023365031893268534
20-21	0.0023365031893268534
22-23	0.0023365031893268534
24-25	0.0023365031893268534
26-27	0.0023365031893268534
28-29	0.0023365031893268534
30-31	0.0023365031893268534
32-33	0.0023365031893268534
34-35	0.0023365031893268534
36-37	0.0023365031893268534
38-39	0.0023365031893268534
40-41	0.0023365031893268534
42-43	0.0023365031893268534
44-45	0.0023365031893268534
46-47	0.0023365031893268534
48-49	0.0023365031893268534
50-51	0.0023365031893268534
52-53	0.0035047547839902804
54-55	0.0023365031893268534
56-57	0.0035047547839902804
58-59	0.0023365031893268534
60-61	0.0023365031893268534
62-63	0.0023365031893268534
64-65	0.0023365031893268534
66-67	0.0023365031893268534
68-69	0.02219678029860511
70-71	0.0023365031893268534
72-73	0.0023365031893268534
74-75	0.0023365031893268534
76	0.0023365031893268534
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	42799.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	99.59812145143579
#Duplication Level	Percentage of deduplicated	Percentage of total
1	99.62934290473174	99.22895394752214
2	0.3378140615103104	0.6729129185261338
3	0.03284303375794684	0.09813313395172785
4	0.0	0.0
5	0.0	0.0
6	0.0	0.0
7	0.0	0.0
8	0.0	0.0
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0023365031893268534	0.0
2	0.0	0.0	0.0	0.0023365031893268534	0.0
3	0.0	0.0	0.0	0.0023365031893268534	0.0
4	0.0	0.0	0.0	0.007009509567980561	0.0
5	0.0	0.0	0.0	0.016355522325287974	0.0
6	0.0	0.0	0.0	0.016355522325287974	0.0
7	0.0	0.0	0.0	0.018692025514614827	0.0
8	0.0	0.0	0.0	0.018692025514614827	0.0
9	0.0	0.0	0.0	0.018692025514614827	0.0
10	0.0	0.0	0.0	0.018692025514614827	0.0
11	0.0	0.0	0.0	0.018692025514614827	0.0
12	0.0	0.0	0.0	0.018692025514614827	0.0
13	0.0	0.0	0.0	0.023365031893268533	0.0
14	0.0	0.0	0.0	0.025701535082595386	0.0
15	0.0	0.0	0.0	0.028038038271922243	0.0
16	0.0	0.0	0.0	0.028038038271922243	0.0
17	0.0	0.0	0.0	0.028038038271922243	0.0
18	0.0	0.0	0.0	0.028038038271922243	0.0
19	0.0	0.0	0.0	0.028038038271922243	0.0
20	0.0	0.0	0.0	0.0350475478399028	0.0
21	0.0	0.0	0.0	0.03972055421855651	0.0
22	0.0	0.0	0.0	0.058412579733171335	0.0
23	0.0	0.0	0.0	0.06308558611182505	0.0
24	0.0	0.0	0.0	0.07944110843711302	0.0
25	0.0	0.0	0.0	0.07944110843711302	0.0
26	0.0	0.0	0.0	0.08645061800509357	0.0
27	0.0	0.0	0.0	0.10747914670903526	0.0
28	0.0	0.0	0.0	0.1401901913596112	0.0
29	0.0	0.0	0.0	0.17757424238884087	0.0
30	0.0	0.0	0.0	0.20327577747143624	0.0
31	0.0	0.0	0.0	0.22897731255403164	0.0
32	0.0	0.0	0.0	0.27337087315124187	0.0
33	0.0	0.0	0.0	0.33879296245239376	0.0
34	0.0	0.0	0.0	0.37384051029229654	0.0
35	0.0	0.0	0.0	0.4813196570013318	0.0
36	0.0	0.0	0.0	0.5887988037103671	0.0
37	0.0	0.0	0.0	0.7990840907497839	0.0
38	0.0	0.0	0.0	1.0117058809785275	0.0
39	0.0	0.0	0.0	1.2710577349938084	0.0
40	0.0	0.0	0.0	1.514054066683801	0.0
41	0.0	0.0	0.0	1.7406948760485057	0.0
42	0.0	0.0	0.0	2.0023832332531133	0.0
43	0.0	0.0	0.0	2.1893034883992617	0.0
44	0.0	0.0	0.0	2.4369728264679082	0.0
45	0.0	0.0	0.0	2.623893081614056	0.0
46	0.0	0.0	0.0	2.834178368653473	0.0
47	0.0	0.0	0.0	3.060819178018178	0.0
48	0.0	0.0	0.0	3.203345872567116	0.0
49	0.0	0.0	0.0	3.3084885160868245	0.0
50	0.0	0.0	0.0	3.46036122339307	0.0
51	0.0	0.0	0.0	3.6776560200004673	0.0
52	0.0	0.0	0.0	3.810836701792098	0.0
53	0.0	0.0	0.0	4.053833033482091	0.0
54	0.0	0.0	0.0	4.252435804574874	0.0
55	0.0	0.0	0.0	4.439356059721021	0.0
56	0.0	0.0	0.0	4.549171709619384	0.0
57	0.0	0.0	0.0	4.6636603658963995	0.0
58	0.0	0.0	0.0	4.7641300030374545	0.0
59	0.0	0.0	0.0	4.885628168882451	0.0
60	0.0	0.0	0.0	4.972078786887544	0.0
61	0.0	0.0	0.0	5.058529404892638	0.0
62	0.0	0.0	0.0	5.1309610037617706	0.0
63	0.0	0.0	0.0	5.208065609009556	0.0
64	0.0	0.0	0.0	5.266478188742727	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCGGTG	95	0.0	70.00001	44
CTAGAAA	15	0.0022102403	70.0	29
TGATACC	15	0.0022102403	70.0	2
GGTGTAG	25	2.7944516E-6	70.0	35
ATGGCGG	20	7.841763E-5	70.0	45
GACGTAA	15	0.0022102403	70.0	23
TCTTAGG	15	0.0022102403	70.0	45
TTGTCAT	20	7.841763E-5	70.0	2
GTCTAGA	15	0.0022102403	70.0	36
CACGAGT	15	0.0022102403	70.0	33
GCGTTGA	15	0.0022102403	70.0	36
AGAGTAG	20	7.841763E-5	70.0	35
TCGTGTA	40	1.2914825E-10	70.0	34
GGTGGAA	15	0.0022102403	70.0	47
TCTAACG	25	2.7944516E-6	70.0	11
ATGACCA	15	0.0022102403	70.0	62
GCTAGAA	15	0.0022102403	70.0	28
GAGCCAG	15	0.0022102403	70.0	48
CGTGTTT	15	0.0022102403	70.0	35
GATCTTG	20	7.841763E-5	70.0	41
>>END_MODULE