FastQCFastQC Report
Wed 25 May 2016
SRR1779727_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779727_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42799
Sequences flagged as poor quality0
Sequence length76
%GC46

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT2153250.3095866725858No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG25876.04453375078857No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC15273.5678403701021053No Hit
ATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCTT9392.1939764947779152Illumina PCR Primer Index 9 (95% over 22bp)
TCTTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC7051.647234748475432No Hit
CTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTC5661.322460805158999No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGACCTCGTATGCCGT4711.100493002172948No Hit
CTTTACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTC3580.8364681417790134No Hit
CTTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG2470.5771162877637328No Hit
TTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTC2100.4906656697586392No Hit
CTTAATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG1890.4415991027827753No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC1810.4229070772681605No Hit
TATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCT1720.40187854856421884Illumina PCR Primer Index 9 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGAACTCGTATGCCGT1660.3878595294282577No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTTAACAGACCTCGTATGCCG1530.35748498796700856No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGAGCTCGTATGCCGT1320.30841842099114464No Hit
CCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG960.22430430617537794No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATATCGTATGCCGT860.20093927428210942No Hit
TCTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT780.18224724876749457No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTTAACAGACCTCGTATGCC620.14486319773826492No Hit
CTTATTACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG500.11682515946634267No Hit
CTCTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG460.10747914670903526No Hit
ATACACATCTCCGAGCCCACGAGACGTTAACAGACCTCGTATGCCGTCTT450.10514264351970841No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTAAT353.601599E-970.000011
ACAAATA150.002210240370.070
AACAAAT150.002210240370.069
GCCAGGT150.002210240370.029
ACAATAA150.002210240370.070
TCTTTAT900.069.999991
AAATAAC352.9082366E-760.00000469
ACTCGTA308.243476E-658.33333238
AACTCGT308.243476E-658.33333237
CTTTATA1100.057.2727242
TTTATAC1100.057.2727243
AACAATA252.365001E-455.99999669
AAACTCC252.365001E-455.99999669
ACACAAA252.365001E-455.99999670
ATAAAAA252.365001E-455.99999670
CTTTACA452.6220732E-854.4444431
AAACCCA200.00690656952.570
CTATACA608.0035534E-1152.51
CTTATTA200.00690656952.51
AATAATA200.00690656952.569
Produced by FastQC (version 0.11.5)