##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779727_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	42799
Sequences flagged as poor quality	0
Sequence length	76
%GC	46
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.66868384775345	34.0	34.0	34.0	34.0	34.0
2	33.665506203415966	34.0	34.0	34.0	34.0	34.0
3	33.67473539101381	34.0	34.0	34.0	34.0	34.0
4	33.71833454052665	34.0	34.0	34.0	34.0	34.0
5	33.750625014603145	34.0	34.0	34.0	34.0	34.0
6	37.462393981167786	38.0	38.0	38.0	37.0	38.0
7	37.43482324353373	38.0	38.0	38.0	37.0	38.0
8	37.355195214841466	38.0	38.0	38.0	37.0	38.0
9	37.38720530853524	38.0	38.0	38.0	37.0	38.0
10-11	37.270286688941326	38.0	38.0	38.0	36.0	38.0
12-13	37.2608355335405	38.0	38.0	38.0	36.0	38.0
14-15	37.27776349914718	38.0	38.0	38.0	36.0	38.0
16-17	37.09395079324283	38.0	38.0	38.0	35.5	38.0
18-19	37.04233743779061	38.0	38.0	38.0	35.0	38.0
20-21	37.17214187247366	38.0	38.0	38.0	36.0	38.0
22-23	37.18508610014253	38.0	38.0	38.0	36.0	38.0
24-25	36.98373793780229	38.0	38.0	38.0	34.5	38.0
26-27	36.77795041940232	38.0	37.5	38.0	34.5	38.0
28-29	36.84463422042571	38.0	37.5	38.0	34.5	38.0
30-31	36.8351363349611	38.0	37.0	38.0	34.5	38.0
32-33	36.594943807098296	38.0	37.0	38.0	34.0	38.0
34-35	36.396013925559004	38.0	37.0	38.0	34.0	38.0
36-37	36.34626977265824	38.0	37.0	38.0	34.0	38.0
38-39	33.4385733311526	37.0	34.0	38.0	23.0	38.0
40-41	35.77023995887754	37.5	36.5	38.0	32.5	38.0
42-43	36.79335965793594	38.0	37.0	38.0	34.5	38.0
44-45	36.84255473258721	38.0	37.5	38.0	34.5	38.0
46-47	36.87323301946307	38.0	37.5	38.0	35.0	38.0
48-49	36.76213229281058	38.0	37.0	38.0	34.5	38.0
50-51	36.53918315848501	38.0	37.0	38.0	34.0	38.0
52-53	36.56362298184537	38.0	37.0	38.0	34.0	38.0
54-55	36.58518890628286	38.0	37.0	38.0	34.0	38.0
56-57	36.55393817612561	38.0	37.0	38.0	34.0	38.0
58-59	36.29865183765976	38.0	37.0	38.0	34.0	38.0
60-61	36.298955583074374	38.0	37.0	38.0	34.0	38.0
62-63	36.50281548634314	38.0	37.0	38.0	34.0	38.0
64-65	36.598413514334446	38.0	37.0	38.0	34.0	38.0
66-67	36.327846445010394	38.0	37.0	38.0	34.0	38.0
68-69	35.68862590247436	38.0	36.5	38.0	31.0	38.0
70-71	30.9659805135634	34.5	28.5	38.0	17.5	38.0
72-73	23.079534568564686	22.5	11.0	35.5	10.0	38.0
74-75	22.059849529194608	22.0	11.0	34.0	10.5	38.0
76	22.15762050515199	22.0	11.0	34.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
22	3.0
23	13.0
24	11.0
25	40.0
26	63.0
27	153.0
28	238.0
29	456.0
30	720.0
31	1087.0
32	1734.0
33	2613.0
34	4899.0
35	13270.0
36	10951.0
37	6548.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	12.00028038038272	5.719759807472137	12.626463235122316	69.65349657702284
2	6.881001892567583	4.362251454473235	71.35213439566344	17.40461225729573
3	6.916049440407486	8.023551952148415	71.80307951120353	13.257319096240566
4	5.944064113647515	58.58080796280287	22.189770789037127	13.285357134512488
5	6.29687609523587	20.41169186195939	66.34500806093601	6.946423981868735
6	10.46286128180565	66.49688076824225	11.759620551882053	11.280637398070049
7	9.327320731792799	18.918666323979533	9.885744994041918	61.86826795018575
8	8.04224397766303	67.54830720343932	6.184723942148182	18.224724876749455
9	7.105306198742961	14.96997593401715	3.969718918666324	73.95499894857358
10-11	9.041099091100259	38.68197855090072	34.0860767774948	18.190845580504217
12-13	9.377555550363326	6.812075048482441	40.37944811794668	43.43092128320755
14-15	10.8413747984766	4.925348723101007	11.43134185378163	72.80193462464077
16-17	41.391153998925205	32.75310170798383	3.9545316479356996	21.901212645155262
18-19	43.02320147667002	12.550526881469192	3.747751115680273	40.67852052618052
20-21	12.883478585948268	5.998971938596696	3.6905067875417648	77.42704268791327
22-23	11.389284796373747	36.802261735087264	3.74658286408561	48.061870604453375
24-25	41.83041659851865	37.34082572022711	3.5386340802355196	17.290123601018713
26-27	44.568798336409735	40.19135961120587	5.2220846281455175	10.017757424238884
28-29	40.312857777050866	12.892824598705577	7.223299609803966	39.57101801443959
30-31	15.75387275403631	7.215121848641323	64.12533003107549	12.905675366246875
32-33	9.331993738171454	65.59615878875674	15.277226103413632	9.79462136965817
34-35	9.759573821818266	44.99754667165121	5.470922217808828	39.7719572887217
36-37	39.608402065468816	40.18551835323255	7.136848991798874	13.069230589499755
38-39	12.542349120306548	11.807518867263255	36.57094791934391	39.07918409308629
40-41	9.384565059931308	5.046846888946003	40.906329587139886	44.6622584639828
42-43	40.132947031472696	5.310871749339937	38.4845440314026	16.071637187784763
44-45	12.08906750157714	35.22512208229164	41.686721652375056	10.999088763756163
46-47	39.7334049860978	7.338956517675646	14.325101053762939	38.60253744246361
48-49	39.00441599102783	4.037477511156803	8.416084487955326	48.54202200986004
50-51	13.938409775929344	4.467394097992944	39.87826818383607	41.71592794224164
52-53	7.826117432650295	4.365756209257226	70.25397789667983	17.554148461412648
54-55	8.073786770718943	4.266454823710834	46.183322040234586	41.47643636533564
56-57	39.06633332554499	4.301502371550738	16.06813243300077	40.5640318699035
58-59	11.725741255636814	7.246664641697236	64.89637608355335	16.131218019112595
60-61	38.11187177270497	37.64340288324494	15.418584546367905	8.82614079768219
62-63	9.947662328559078	76.75296151779247	4.790999789714713	8.508376363933737
64-65	6.462767821678077	80.43879529895558	4.442860814505012	8.655576064861329
66-67	6.333091894670437	80.61636954134443	4.133274141919204	8.917264422065935
68-69	6.758335475127923	79.93527886165565	4.550339961214047	8.756045702002384
70-71	7.386854833056847	73.70499310731559	6.406691745134233	12.501460314493329
72-73	9.357695273254048	57.40087385219282	12.46758101824809	20.773849856305056
74-75	10.546975396621416	50.08177761162644	15.334470431552138	24.036776560200003
76	10.481553307320263	51.3002640248604	14.73866211827379	23.479520549545548
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.5
17	1.0
18	1.0
19	0.5
20	1.0
21	2.0
22	2.5
23	2.0
24	2.5
25	4.0
26	4.0
27	6.5
28	10.0
29	11.0
30	14.5
31	24.5
32	22.5
33	14.0
34	20.0
35	28.5
36	40.0
37	49.0
38	208.0
39	4618.0
40	9396.5
41	8575.0
42	7226.0
43	5628.0
44	2903.5
45	1243.5
46	710.0
47	494.0
48	213.5
49	130.0
50	111.0
51	108.5
52	112.5
53	120.5
54	122.0
55	158.5
56	216.0
57	282.5
58	328.0
59	372.5
60	441.5
61	491.5
62	517.0
63	530.5
64	559.5
65	577.5
66	551.5
67	523.0
68	535.5
69	564.0
70	544.5
71	509.0
72	525.5
73	497.0
74	440.0
75	428.0
76	414.5
77	334.0
78	241.0
79	215.0
80	196.0
81	143.5
82	102.5
83	95.0
84	69.0
85	36.0
86	23.0
87	17.0
88	10.0
89	5.5
90	4.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	42799.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.974719035491482
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.97088262056415	12.217575176990117
2	20.187183153516184	7.257178906049207
3	7.149356557909788	3.8552302623893078
4	2.378785909268166	1.7103203345872569
5	0.8839204471597556	0.7944110843711302
6	0.15598596126348627	0.16822822963153344
7	0.1689847913687768	0.21262179022874367
8	0.09099181073703368	0.1308441786023038
9	0.09099181073703368	0.14719970092759177
>10	0.6629403353698167	2.343512698894834
>50	0.0519953204211621	0.7523540269632468
>100	0.12998830105290524	5.324890768475899
>500	0.03899649031587157	5.163672048412346
>1k	0.02599766021058105	9.612374120890674
>5k	0.0	0.0
>10k+	0.012998830105290526	50.3095866725858
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT	21532	50.3095866725858	No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG	2587	6.04453375078857	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC	1527	3.5678403701021053	No Hit
ATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCTT	939	2.1939764947779152	Illumina PCR Primer Index 9 (95% over 22bp)
TCTTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC	705	1.647234748475432	No Hit
CTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTC	566	1.322460805158999	No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGACCTCGTATGCCGT	471	1.100493002172948	No Hit
CTTTACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTC	358	0.8364681417790134	No Hit
CTTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG	247	0.5771162877637328	No Hit
TTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTC	210	0.4906656697586392	No Hit
CTTAATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG	189	0.4415991027827753	No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC	181	0.4229070772681605	No Hit
TATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCT	172	0.40187854856421884	Illumina PCR Primer Index 9 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGAACTCGTATGCCGT	166	0.3878595294282577	No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTTAACAGACCTCGTATGCCG	153	0.35748498796700856	No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGAGCTCGTATGCCGT	132	0.30841842099114464	No Hit
CCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG	96	0.22430430617537794	No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATATCGTATGCCGT	86	0.20093927428210942	No Hit
TCTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT	78	0.18224724876749457	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTTAACAGACCTCGTATGCC	62	0.14486319773826492	No Hit
CTTATTACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG	50	0.11682515946634267	No Hit
CTCTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG	46	0.10747914670903526	No Hit
ATACACATCTCCGAGCCCACGAGACGTTAACAGACCTCGTATGCCGTCTT	45	0.10514264351970841	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0023365031893268534	0.0
2	0.0	0.0	0.0	0.0023365031893268534	0.0
3	0.0	0.0	0.0	0.004673006378653707	0.0
4	0.0	0.0	0.0	0.009346012757307414	0.0
5	0.0	0.0	0.0	0.023365031893268533	0.0
6	0.0	0.0	0.0	0.023365031893268533	0.0
7	0.0	0.0	0.0	0.028038038271922243	0.0
8	0.0	0.0	0.0	0.028038038271922243	0.0
9	0.0	0.0	0.0	0.028038038271922243	0.0
10	0.0	0.0	0.0	0.030374541461249096	0.0
11	0.0	0.0	0.0	0.030374541461249096	0.0
12	0.0	0.0	0.0	0.030374541461249096	0.0
13	0.0	0.0	0.0	0.04205705740788336	0.0
14	0.0	0.0	0.0	0.04906656697586392	0.0
15	0.0	0.0	0.0	0.05373957335451763	0.0
16	0.0	0.0	0.0	0.05373957335451763	0.0
17	0.0	0.0	0.0	0.056076076543844486	0.0
18	0.0	0.0	0.0	0.056076076543844486	0.0
19	0.0	0.0	0.0	0.056076076543844486	0.0
20	0.0	0.0	0.0	0.0654220893011519	0.0
21	0.0	0.0	0.0	0.07243159886913246	0.0
22	0.0	0.0	0.0	0.08411411481576672	0.0
23	0.0	0.0	0.0	0.09346012757307413	0.0
24	0.0	0.0	0.0	0.095796630762401	0.0
25	0.0	0.004673006378653707	0.0	0.095796630762401	0.0
26	0.0	0.004673006378653707	0.0	0.10514264351970841	0.0
27	0.0	0.004673006378653707	0.0	0.13318068179163065	0.0
28	0.0	0.004673006378653707	0.0	0.17991074557816772	0.0
29	0.0	0.004673006378653707	0.0	0.2593518540152807	0.0
30	0.0	0.004673006378653707	0.0	0.28972639547652984	0.0
31	0.0	0.004673006378653707	0.0	0.37384051029229654	0.0
32	0.0	0.004673006378653707	0.0	0.462627631486717	0.0
33	0.0	0.004673006378653707	0.0	0.5771162877637328	0.0
34	0.0	0.004673006378653707	0.0	0.7102969695553635	0.0
35	0.0	0.004673006378653707	0.0	0.8972172247015118	0.0
36	0.0	0.004673006378653707	0.0	1.179934110610061	0.0
37	0.0	0.004673006378653707	0.0	1.5117175634944742	0.0
38	0.0	0.004673006378653707	0.0	1.8598565387041752	0.0
39	0.0	0.004673006378653707	0.0	2.247716068132433	0.0
40	0.0	0.004673006378653707	0.0	2.602864552910115	0.0
41	0.0	0.004673006378653707	0.0	2.929974999415874	0.0
42	0.0	0.004673006378653707	0.0	3.278113974625575	0.0
43	0.0	0.004673006378653707	0.0	3.5117642935582607	0.0
44	0.0	0.004673006378653707	0.0	3.8318652304960397	0.0
45	0.0	0.004673006378653707	0.0	4.130937638729877	0.0
46	0.0	0.004673006378653707	0.0	4.46973060118227	0.0
47	0.0	0.004673006378653707	0.0	4.808523563634664	0.0
48	0.0	0.004673006378653707	0.0	5.032827869810042	0.0
49	0.0	0.004673006378653707	0.0	5.245449660038786	0.0
50	0.0	0.004673006378653707	0.0	5.4323699151849345	0.0
51	0.0	0.004673006378653707	0.0	5.668356737306946	0.0
52	0.0	0.004673006378653707	0.0	5.8249024509918454	0.0
53	0.0	0.004673006378653707	0.0	6.0819178018177995	0.0
54	0.0	0.004673006378653707	0.0	6.25949204420664	0.0
55	0.0	0.004673006378653707	0.0	6.476786840814038	0.0
56	0.0	0.004673006378653707	0.0	6.635669057688264	0.0
57	0.0	0.004673006378653707	0.0	6.834271828781047	0.0
58	0.0	0.004673006378653707	0.0	6.997827052033926	0.0
59	0.0	0.004673006378653707	0.0	7.168391784854786	0.0
60	0.0	0.004673006378653707	0.0	7.329610504918339	0.0
61	0.0	0.004673006378653707	0.0	7.558587817472371	0.0
62	0.0	0.004673006378653707	0.0	7.7291525502932314	0.0
63	0.0	0.004673006378653707	0.0	7.876352251220823	0.0
64	0.0	0.004673006378653707	0.0	8.032897964905722	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTTAAT	35	3.601599E-9	70.00001	1
ACAAATA	15	0.0022102403	70.0	70
AACAAAT	15	0.0022102403	70.0	69
GCCAGGT	15	0.0022102403	70.0	29
ACAATAA	15	0.0022102403	70.0	70
TCTTTAT	90	0.0	69.99999	1
AAATAAC	35	2.9082366E-7	60.000004	69
ACTCGTA	30	8.243476E-6	58.333332	38
AACTCGT	30	8.243476E-6	58.333332	37
CTTTATA	110	0.0	57.272724	2
TTTATAC	110	0.0	57.272724	3
AACAATA	25	2.365001E-4	55.999996	69
AAACTCC	25	2.365001E-4	55.999996	69
ACACAAA	25	2.365001E-4	55.999996	70
ATAAAAA	25	2.365001E-4	55.999996	70
CTTTACA	45	2.6220732E-8	54.444443	1
AAACCCA	20	0.006906569	52.5	70
CTATACA	60	8.0035534E-11	52.5	1
CTTATTA	20	0.006906569	52.5	1
AATAATA	20	0.006906569	52.5	69
>>END_MODULE