##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779726_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	52898
Sequences flagged as poor quality	0
Sequence length	76
%GC	55
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	23.541116866422172	23.0	12.0	33.0	12.0	34.0
2	23.800672993307874	23.0	12.0	33.0	12.0	34.0
3	23.428314870127416	23.0	11.0	32.0	11.0	34.0
4	23.268857045634995	23.0	12.0	33.0	11.0	34.0
5	23.690649930054068	23.0	11.0	34.0	11.0	34.0
6	24.494253090854098	26.0	12.0	36.0	11.0	38.0
7	25.500056712919204	27.0	12.0	36.0	12.0	38.0
8	24.601648455518166	26.0	11.0	36.0	11.0	38.0
9	26.327157926575673	28.0	12.0	37.0	12.0	38.0
10-11	25.350750500964118	27.0	11.0	37.0	11.0	38.0
12-13	25.746833528677833	27.5	11.0	36.0	11.0	38.0
14-15	24.617339029830994	26.0	11.0	35.5	11.0	38.0
16-17	24.709270671859052	26.5	11.0	35.5	11.0	38.0
18-19	25.0965537449431	26.5	11.5	36.0	11.0	38.0
20-21	24.315106431245038	25.5	11.0	35.0	11.0	38.0
22-23	24.479318688797306	25.0	11.0	35.5	11.0	38.0
24-25	24.510104351771332	25.5	11.0	35.0	11.0	38.0
26-27	23.6609890733109	23.0	11.0	34.0	10.0	38.0
28-29	24.50684335891716	26.0	11.0	36.0	11.0	38.0
30-31	24.916405157094786	26.0	11.0	37.0	11.0	38.0
32-33	25.12671556580589	26.0	11.0	37.0	11.0	38.0
34-35	25.21135959771636	26.5	11.0	37.0	11.0	38.0
36-37	24.414136640326667	25.0	11.0	35.0	11.0	38.0
38-39	24.185904949147414	25.5	11.0	34.0	11.0	37.5
40-41	23.691415554463305	25.0	11.0	34.0	11.0	37.5
42-43	24.234186547695565	26.0	11.0	35.0	11.0	38.0
44-45	25.223912057166622	26.5	11.0	37.0	11.0	38.0
46-47	24.68959128889561	26.0	11.0	35.5	11.0	38.0
48-49	23.92084766909902	25.0	11.0	35.0	11.0	38.0
50-51	23.13045861847329	23.0	11.0	34.0	11.0	37.5
52-53	24.123397860032515	25.0	11.0	35.0	11.0	38.0
54-55	23.921717267193465	24.0	11.0	35.0	11.0	38.0
56-57	24.247627509546675	24.5	11.0	35.5	11.0	38.0
58-59	24.608038111081704	25.5	11.0	36.5	11.0	38.0
60-61	25.155402850769406	26.0	11.0	37.0	11.0	38.0
62-63	25.136413474989602	26.5	11.0	37.0	11.0	38.0
64-65	25.049869560285835	26.5	11.0	37.0	11.0	38.0
66-67	24.17442058300881	25.0	11.0	34.5	11.0	38.0
68-69	23.92937351128587	25.0	11.0	34.5	11.0	38.0
70-71	23.867348481984195	25.0	11.0	34.0	11.0	38.0
72-73	23.522099134182767	24.0	11.0	34.0	11.0	37.5
74-75	23.563764225490566	24.5	11.0	34.5	11.0	37.5
76	23.273186131800824	24.0	11.0	34.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	fail
#Quality	Count
6	1.0
7	0.0
8	0.0
9	0.0
10	0.0
11	5.0
12	67.0
13	363.0
14	949.0
15	1532.0
16	2652.0
17	3913.0
18	5123.0
19	5143.0
20	4341.0
21	3301.0
22	2348.0
23	1697.0
24	1266.0
25	1121.0
26	1034.0
27	957.0
28	980.0
29	1061.0
30	1168.0
31	1425.0
32	1619.0
33	1976.0
34	2330.0
35	2742.0
36	2593.0
37	1191.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.471775870543308	14.70187908805626	13.204658021097208	43.62168702030323
2	16.560172407274376	11.628038867253961	47.9375401716511	23.87424855382056
3	15.282241294566903	12.036371885515521	47.99803395213429	24.68335286778328
4	15.771862830352754	22.4356308367046	23.537751899882796	38.25475443305985
5	14.69998865741616	19.59809444591478	44.12643200120988	21.575484895459184
6	25.10491890052554	32.6496275851639	14.524178607886876	27.72127490642368
7	23.4678059661991	19.27672123709781	16.524254225112482	40.73121857159061
8	18.92734937709133	32.91869104107983	17.426319072915287	30.727640508913552
9	19.203357468287425	13.675633778853244	16.54536174074144	50.575647012117884
10-11	21.187401932056638	20.794184925421103	28.041287785696735	29.97712535682553
12-13	19.186887353366544	13.902883988240966	33.74767229726536	33.16255636112713
14-15	25.68552389054303	20.01701403658018	21.322368731981662	32.97509334089513
16-17	30.73011191772535	14.770493042952207	21.216160012099213	33.28323502722323
18-19	19.99319412804492	14.194969326313203	29.729372063786148	36.08246448185574
20-21	30.0486790491044	14.399546292357861	22.57857176615152	32.97320289238622
22-23	32.27782293891903	13.175605421857572	20.817815755146796	33.7287558840766
24-25	21.177749421050144	21.271326622241126	19.103927406777256	38.44699654993147
26-27	28.744269577957372	20.51042109740536	21.5246467224349	29.220662602202374
28-29	23.373001994460882	19.589197773009555	22.67163233862354	34.366167893906024
30-31	21.92814133527427	19.585787070733808	24.66655953720071	33.81951205679122
32-33	23.279643073200244	18.303841500302482	24.793935269207502	33.622580157289775
34-35	22.255936176648518	18.647912885662432	24.238127646702964	34.85802329098608
36-37	29.20920296430732	13.16167574107683	30.05520266182698	27.573918632788867
38-39	28.782622668796616	10.970480088474662	36.28122843666405	23.965668806064674
40-41	24.24024728001437	22.428183871973985	23.0700154077379	30.261553440273747
42-43	17.365043055778738	22.85217358426361	27.420528768444036	32.36225459151362
44-45	17.42319689951791	15.185745344550524	32.699688061253426	34.69136969467814
46-47	27.867628292987252	14.489616515270388	26.670006522170652	30.972748669571708
48-49	26.240169388989713	15.31495765275257	30.644094071385357	27.80077888687235
50-51	38.72903945403331	13.22664801406507	24.426716070854678	23.61759646104694
52-53	16.34244555353902	19.126209921355112	30.69041137326074	33.840933151845135
54-55	25.47781537705352	16.051950016068965	25.535474601584212	32.934760005293306
56-57	25.46695402298851	17.13929219600726	24.129423774954628	33.26433000604961
58-59	17.2875332016296	20.33877477715917	28.724017657122875	33.64967436408836
60-61	17.16986482654315	17.56498723886946	29.06985537385386	36.19529256073353
62-63	17.193334026523996	20.337268765774024	28.103524874516733	34.365872333185244
64-65	17.453730835397092	21.513507382271207	26.87865096319262	34.154110819139085
66-67	15.050429620667163	32.42147252601828	18.547891597583916	33.980206255730636
68-69	16.11908971437756	30.689521504410557	18.61320424699108	34.5781845342208
70-71	14.99357229280097	32.18768905021174	17.176156987295826	35.64258166969147
72-73	13.637953361753613	36.8720047640203	15.603111737071451	33.88693013715463
74-75	16.727633631078973	25.91804905713881	20.106810340753345	37.247506971028876
76	16.605856664839216	22.90489063652003	21.413312664234265	39.075940034406486
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.5
10	0.5
11	0.0
12	0.0
13	0.0
14	1.0
15	1.0
16	0.5
17	1.0
18	1.0
19	2.0
20	7.5
21	12.0
22	15.5
23	20.0
24	26.5
25	32.0
26	36.5
27	59.5
28	95.5
29	113.0
30	138.0
31	204.5
32	299.0
33	352.0
34	434.0
35	579.5
36	715.5
37	788.0
38	860.5
39	1043.5
40	1303.0
41	1549.0
42	1646.0
43	1712.0
44	1871.5
45	1992.0
46	2019.0
47	2107.0
48	2178.5
49	2133.5
50	2105.0
51	2073.5
52	2015.0
53	1936.0
54	1884.0
55	1876.5
56	1842.5
57	1840.5
58	1865.0
59	1908.0
60	1925.5
61	1942.5
62	1985.0
63	1931.5
64	1849.5
65	1768.0
66	1655.5
67	1596.0
68	1558.0
69	1421.5
70	1218.0
71	1113.0
72	1023.0
73	883.5
74	752.5
75	671.0
76	616.0
77	486.5
78	350.0
79	288.0
80	242.0
81	157.0
82	98.0
83	78.0
84	61.5
85	36.0
86	21.0
87	15.0
88	9.5
89	2.5
90	2.5
91	2.0
92	0.0
93	1.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0018904306400998148
9	0.0018904306400998148
10-11	0.0018904306400998148
12-13	0.004726076600249537
14-15	9.452153200499074E-4
16-17	0.0037808612801996295
18-19	0.004726076600249537
20-21	9.452153200499074E-4
22-23	0.0018904306400998148
24-25	9.452153200499074E-4
26-27	9.452153200499074E-4
28-29	0.0028356459601497222
30-31	0.004726076600249537
32-33	0.0037808612801996295
34-35	0.0037808612801996295
36-37	0.0037808612801996295
38-39	0.0028356459601497222
40-41	0.004726076600249537
42-43	0.0028356459601497222
44-45	0.0056712919202994445
46-47	0.0028356459601497222
48-49	0.0037808612801996295
50-51	0.0018904306400998148
52-53	0.0037808612801996295
54-55	0.0018904306400998148
56-57	0.0037808612801996295
58-59	0.0028356459601497222
60-61	0.0056712919202994445
62-63	0.004726076600249537
64-65	0.0018904306400998148
66-67	0.004726076600249537
68-69	0.025520813641347503
70-71	0.0037808612801996295
72-73	0.0028356459601497222
74-75	9.452153200499074E-4
76	0.0018904306400998148
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	52898.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	99.48580286589285
#Duplication Level	Percentage of deduplicated	Percentage of total
1	99.53635085319044	99.0245377897085
2	0.4218447155398472	0.8393512042043176
3	0.03040322274161061	0.09074067072479111
4	0.011401208528103978	0.045370335362395556
5	0.0	0.0
6	0.0	0.0
7	0.0	0.0
8	0.0	0.0
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.007561722560399259	0.0
5	0.0	0.0	0.0	0.007561722560399259	0.0
6	0.0	0.0	0.0	0.007561722560399259	0.0
7	0.0	0.0	0.0	0.007561722560399259	0.0
8	0.0	0.0	0.0	0.009452153200499074	0.0
9	0.0	0.0	0.0	0.009452153200499074	0.0
10	0.0	0.0	0.0	0.011342583840598889	0.0
11	0.0	0.0	0.0	0.011342583840598889	0.0
12	0.0	0.0	0.0	0.015123445120798518	0.0
13	0.0	0.0	0.0	0.020794737041097962	0.0
14	0.0	0.0	0.0	0.024575598321297593	0.0
15	0.0	0.0	0.0	0.02835645960149722	0.0
16	0.0	0.0	0.0	0.03213732088169685	0.0
17	0.0	0.0	0.0	0.03213732088169685	0.0
18	0.0	0.0	0.0	0.03591818216189648	0.0
19	0.0	0.0	0.0	0.037808612801996294	0.0
20	0.0	0.0	0.0	0.037808612801996294	0.0
21	0.0	0.0	0.0	0.039699043442096106	0.0
22	0.0	0.0	0.0	0.051041627282695	0.0
23	0.0	0.0	0.0	0.05482248856289463	0.0
24	0.0	0.0	0.0	0.06049378048319407	0.0
25	0.0	0.0	0.0	0.07372679496389277	0.0
26	0.0	0.0	0.0	0.08128851752429203	0.0
27	0.0	0.0	0.0	0.1285492835267874	0.0
28	0.0	0.0	0.0	0.15879617376838442	0.0
29	0.0	0.0	0.0	0.19660478657038075	0.0
30	0.0	0.0	0.0	0.25142727513327534	0.0
31	0.0	0.0	0.0	0.276002873454573	0.0
32	0.0	0.0	0.0	0.3402775152179667	0.0
33	0.0	0.0	0.0	0.37997655866006275	0.0
34	0.0	0.0	0.0	0.4688267987447541	0.0
35	0.0	0.0	0.0	0.6351846950735377	0.0
36	0.0	0.0	0.0	0.8450224961246172	0.0
37	0.0	0.0	0.0	1.3100684335891717	0.0
38	0.0	0.0	0.0	1.73541532761163	0.0
39	0.0	0.0	0.0	2.223146432757382	0.0
40	0.0	0.0	0.0	2.712767968543234	0.0
41	0.0	0.0	0.0	3.111648833604295	0.0
42	0.0	0.0	0.0	3.519981851865855	0.0
43	0.0	0.0	0.0	3.9472191765284133	0.0
44	0.0	0.0	0.0	4.446292865514764	0.0
45	0.0	0.0	0.0	4.729857461529736	0.0
46	0.0	0.0	0.0	5.0984914363492	0.0018904306400998148
47	0.0	0.0	0.0	5.453892396687966	0.0018904306400998148
48	0.0	0.0	0.0	5.7336761314227385	0.0018904306400998148
49	0.0	0.0	0.0	5.992665129116412	0.0018904306400998148
50	0.0	0.0	0.0	6.33105221369428	0.0018904306400998148
51	0.0	0.0	0.0	6.758289538356838	0.0018904306400998148
52	0.0	0.0	0.0	7.0267306892510115	0.0018904306400998148
53	0.0	0.0	0.0	7.391583802790276	0.0018904306400998148
54	0.0	0.0	0.0	7.6770388294453475	0.0018904306400998148
55	0.0	0.0	0.0	7.947370410979621	0.0018904306400998148
56	0.0	0.0	0.0	8.1761125184317	0.0018904306400998148
57	0.0	0.0	0.0	8.406745056523876	0.0018904306400998148
58	0.0	0.0	0.0	8.654391470376952	0.0018904306400998148
59	0.0	0.0	0.0	8.869900563348331	0.0018904306400998148
60	0.0	0.0	0.0	8.98899769367462	0.0018904306400998148
61	0.0	0.0	0.0	9.161026881923702	0.0018904306400998148
62	0.0	0.0	0.0	9.268781428409392	0.0018904306400998148
63	0.0	0.0	0.0	9.38031683617528	0.0018904306400998148
64	0.0	0.0	0.0	9.518318272902567	0.0018904306400998148
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTAGAT	20	7.862632E-5	69.99338	37
TCTTGGT	35	3.6252459E-9	69.99338	43
TAACGCT	25	2.80522E-6	69.99338	13
CTTGGTG	35	3.6252459E-9	69.99338	44
ATGTATA	20	7.862632E-5	69.99338	28
ATCTTGG	25	2.80522E-6	69.99338	42
TGCCTAC	75	0.0	69.99338	19
CGTAACA	20	7.862632E-5	69.99338	56
GTGGTAG	40	1.3096724E-10	69.99338	48
AAGTGTA	25	2.80522E-6	69.99338	34
AGATCTT	15	0.0022139775	69.99337	40
CTCCGTA	15	0.0022139775	69.99337	53
TTAGACT	15	0.0022139775	69.99337	38
ACCGACG	15	0.0022139775	69.99337	20
ATATGTG	15	0.0022139775	69.99337	32
ATATGTA	15	0.0022139775	69.99337	26
GTCTAGA	15	0.0022139775	69.99337	36
CAATTAT	15	0.0022139775	69.99337	56
GCCATAT	15	0.0022139775	69.99337	23
GCCGAAA	15	0.0022139775	69.99337	23
>>END_MODULE