FastQCFastQC Report
Wed 25 May 2016
SRR1779726_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779726_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52898
Sequences flagged as poor quality0
Sequence length76
%GC51

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT2181441.237853983137356No Hit
TCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG25164.756323490491134No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC14762.7902756247873266No Hit
ATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCTT8121.5350296797610496RNA PCR Primer, Index 42 (95% over 22bp)
TCTTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC7091.3403153238307688No Hit
CTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTC6161.1645052743014859RNA PCR Primer, Index 32 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACATCACTCGACCTCGTATGCCGT4850.9168588604484101No Hit
CTTTACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTC3710.7013497674770313No Hit
CTTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG2460.46504593746455447No Hit
TCTTAATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC2360.44614163106355625No Hit
TTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTC1930.36485311353926425RNA PCR Primer, Index 32 (95% over 21bp)
CTTAATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG1800.3402775152179667No Hit
TATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCT1790.3383870845778668RNA PCR Primer, Index 42 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACATCACTCGAACTCGTATGCCGT1640.3100306249763696No Hit
TCTTATACACATCTCCGAGCCCACGAGACATCACTCGACCTCGTATGCCG1620.30624976369617No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGAGCTCGTATGCCGT1350.255208136413475No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATATCGTATGCCGT1280.2419751219327763No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACCCGATCTCGTATGCCGT1010.19093349465008128No Hit
CCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG800.15123445120798518No Hit
TCTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT790.14934402056788537No Hit
CTTATACACATCTCCGAGCCCACGAGACATCCCTCGATCTCGTATGCCGT620.11720669968618852No Hit
CTCTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG530.10019282392529018No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATATC207.858949E-570.035
AATCTAA207.858949E-570.070
TCGATAT207.858949E-570.034
GATATCG207.858949E-570.036
TATCGTA207.858949E-570.038
GAAAAAC150.002213146570.060
GCTCGTA252.8036447E-670.038
AACAGAA150.002213146570.070
TTTACAC401.3096724E-1070.02
ATATCGT207.858949E-570.037
AATAAAC150.002213146570.070
TCTTAAT301.0054828E-770.01
CTCGATA207.858949E-570.033
TCTTTAT900.066.111111
ATCGTAT352.921588E-760.039
AAACAGA308.270461E-658.33333269
TTTATAC1100.057.2727243
CTTTATA1050.056.666672
AAATCTA252.3701537E-455.99999669
AACAAAT200.006915578652.570
Produced by FastQC (version 0.11.5)