##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779726_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	52898
Sequences flagged as poor quality	0
Sequence length	76
%GC	51
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.65730273356271	34.0	34.0	34.0	34.0	34.0
2	33.634334001285495	34.0	34.0	34.0	34.0	34.0
3	33.655847101969826	34.0	34.0	34.0	34.0	34.0
4	33.708079700555786	34.0	34.0	34.0	34.0	34.0
5	33.722428069114144	34.0	34.0	34.0	34.0	34.0
6	37.39846497032024	38.0	38.0	38.0	37.0	38.0
7	37.408295209648756	38.0	38.0	38.0	37.0	38.0
8	37.30052175885667	38.0	38.0	38.0	36.0	38.0
9	37.32182691217059	38.0	38.0	38.0	37.0	38.0
10-11	37.19263488222617	38.0	38.0	38.0	36.0	38.0
12-13	37.20217210480547	38.0	38.0	38.0	36.0	38.0
14-15	37.222853416008164	38.0	38.0	38.0	36.0	38.0
16-17	37.08005028545503	38.0	38.0	38.0	35.5	38.0
18-19	37.0473741918409	38.0	38.0	38.0	35.0	38.0
20-21	37.11703656092858	38.0	38.0	38.0	35.5	38.0
22-23	37.13529812091194	38.0	38.0	38.0	36.0	38.0
24-25	36.999499035880376	38.0	38.0	38.0	35.0	38.0
26-27	36.86382282884041	38.0	37.5	38.0	34.5	38.0
28-29	36.70969601875307	38.0	37.0	38.0	34.0	38.0
30-31	36.47017845665242	38.0	37.0	38.0	34.0	38.0
32-33	36.2992079095618	38.0	37.0	38.0	34.0	38.0
34-35	36.03145676585126	38.0	37.0	38.0	33.0	38.0
36-37	36.30894362735831	38.0	37.0	38.0	34.0	38.0
38-39	33.81657151499111	37.0	34.0	38.0	24.0	38.0
40-41	35.88496729554993	37.5	36.5	38.0	32.5	38.0
42-43	36.76798744754055	38.0	37.0	38.0	34.5	38.0
44-45	36.80394343831525	38.0	37.0	38.0	34.5	38.0
46-47	36.84720594351393	38.0	37.0	38.0	35.0	38.0
48-49	36.765633861393624	38.0	37.0	38.0	34.5	38.0
50-51	36.570607584407725	38.0	37.0	38.0	34.0	38.0
52-53	36.57264924949904	38.0	37.0	38.0	34.0	38.0
54-55	36.597508412416346	38.0	37.0	38.0	34.0	38.0
56-57	36.57470981889675	38.0	37.0	38.0	34.0	38.0
58-59	36.36139362546788	38.0	37.0	38.0	34.0	38.0
60-61	36.367991228401834	38.0	37.0	38.0	34.0	38.0
62-63	36.48728685394533	38.0	37.0	38.0	34.0	38.0
64-65	36.564057242239784	38.0	37.0	38.0	34.0	38.0
66-67	36.3465631970963	38.0	37.0	38.0	34.0	38.0
68-69	35.81874551022723	38.0	36.5	38.0	31.0	38.0
70-71	31.951207985179025	36.0	29.0	38.0	19.0	38.0
72-73	25.466208552308217	24.0	11.0	38.0	10.0	38.0
74-75	24.68787099701312	23.5	11.0	38.0	11.0	38.0
76	24.492476086052402	24.0	11.0	37.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	2.0
21	3.0
22	2.0
23	8.0
24	19.0
25	44.0
26	91.0
27	193.0
28	278.0
29	502.0
30	758.0
31	1336.0
32	2053.0
33	3260.0
34	5830.0
35	14335.0
36	11833.0
37	12350.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.225679609815117	6.508752693863662	11.42009149684298	62.84547619947824
2	10.737646035766947	6.113652690082801	62.716926915951454	20.431774358198798
3	10.826496275851639	8.592007259253657	62.412567582895385	18.168928881999317
4	8.57121252221256	50.14745358992778	21.592498771220082	19.68883511663957
5	9.420015879617377	20.473363832280995	59.126999130401906	10.979621157699725
6	17.252070021550907	58.70543309765964	11.079813981625014	12.96268289916443
7	15.165034594880714	18.05172218231313	9.811335022118039	56.97190820068811
8	13.030738402208023	59.96824076524633	7.264924949903588	19.736095882642065
9	10.949374267458127	13.792581950168248	5.397179477484971	69.86086430488866
10-11	14.516616885326478	34.521153918862716	30.646716321978147	20.31551287383266
12-13	13.712238647964007	7.610873757041854	36.268857045634995	42.40803054935915
14-15	14.493931717645282	6.22613331316874	11.52217475140837	67.7577602177776
16-17	40.70097168134901	29.945366554501113	5.553140005293206	23.800521758856668
18-19	42.04884872774018	12.340731218571591	5.536126129532307	40.074293924155924
20-21	16.51574728723203	7.3792960036296265	5.649551967938296	70.45540474120004
22-23	15.663163068547014	32.98990510038187	5.334795266361677	46.01213656470944
24-25	41.28133388785965	33.14113955158985	5.227040719875988	20.350485840674505
26-27	41.866422170970544	36.89553480282808	6.518204847064162	14.719838179137207
28-29	15.687738666868311	36.61953192937351	7.199705092820144	40.49302431093803
30-31	13.066656584369921	13.15267117849446	32.61654504896215	41.16412718817347
32-33	13.538319029074824	32.049415856932214	10.41060153502968	44.00166357896329
34-35	14.233997504631555	9.854814926840334	32.6912170592461	43.219970509282014
36-37	38.542099890355026	29.461416310635563	11.170554652349805	20.825929146659607
38-39	16.10552383833037	11.84354796022534	33.36893644372188	38.681991757722415
40-41	13.537373813754774	6.216681159968241	37.14979772392151	43.096147302355476
42-43	38.64040228364021	6.598548149268403	35.44179364059133	19.319255926500055
44-45	14.889031721426141	31.709138341714244	38.55722333547582	14.844606601383795
46-47	37.8199553858369	8.596733335853909	15.8162879503951	37.7670233279141
48-49	36.738629059699804	6.370751257136376	10.899277855495482	45.99134182766834
50-51	15.577148474422472	7.0040455215698145	36.89648001814814	40.52232598585958
52-53	10.72252259064615	6.797988581798934	62.2386479640062	20.24084086354872
54-55	10.80948240009074	6.687398389353094	42.007259253657985	40.49585995689819
56-57	36.18662331279066	6.780974706038036	16.885326477371546	40.14707550379977
58-59	14.095996067904268	8.938901281711974	57.623161556202504	19.341941094181255
60-61	36.16015728382926	34.100533101440504	15.935385080721387	13.803924534008846
62-63	12.158304661801958	66.43823963098794	7.157170403417899	14.246285303792202
64-65	9.669552724110552	69.46292865514764	6.614616809709252	14.252901811032553
66-67	10.042912775530265	69.72947937540171	6.259215849370486	13.96839199969753
68-69	10.054255359370865	69.67182124087867	6.440697190820069	13.833226208930395
70-71	10.450300578471776	64.78127717494046	8.095769216227456	16.672653030360316
72-73	11.395515898521683	51.24390336118568	13.012779311127073	24.347801429165564
74-75	12.371923324133236	45.09905856554123	15.50720254073878	27.021815569586753
76	12.416348444175583	45.14159325494347	15.361639381451095	27.080418919429842
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.5
17	1.0
18	0.5
19	0.5
20	1.0
21	1.0
22	4.0
23	5.5
24	4.0
25	4.0
26	3.0
27	7.0
28	12.5
29	13.0
30	13.0
31	18.0
32	31.0
33	39.0
34	38.0
35	38.0
36	46.5
37	54.0
38	64.0
39	249.0
40	4685.5
41	9488.0
42	10029.0
43	8693.0
44	5737.0
45	2997.0
46	1877.0
47	1294.5
48	504.0
49	246.5
50	197.0
51	165.0
52	163.0
53	205.0
54	217.0
55	293.5
56	420.5
57	543.5
58	616.0
59	757.5
60	954.5
61	1081.0
62	1152.0
63	1227.5
64	1295.0
65	1276.0
66	1239.0
67	1213.0
68	1204.5
69	1164.0
70	1101.0
71	1070.0
72	993.5
73	880.5
74	814.5
75	785.0
76	729.0
77	634.5
78	506.0
79	416.0
80	348.5
81	248.0
82	198.0
83	181.0
84	133.5
85	73.0
86	41.0
87	22.0
88	15.5
89	7.5
90	3.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	52898.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.5151423494272
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.132547973623765	16.10835948429052
2	22.88656738534489	11.679080494536656
3	8.772319774764762	6.714809633634541
4	3.2377565384900353	3.3044727588944762
5	0.9705860561606282	1.2382320692653788
6	0.288953100689042	0.44236076978335664
7	0.11113580795732383	0.19849521721048055
8	0.05927243091057272	0.12098756096638814
9	0.037045269319107954	0.08506937880449167
>10	0.34081647773579316	1.8885402094597148
>50	0.02963621545528636	0.5179779953873492
>100	0.08890864636585907	4.8773110514575215
>500	0.02222716159146477	4.039850277893304
>1k	0.01481810772764318	7.54659911527846
>5k	0.0	0.0
>10k+	0.00740905386382159	41.237853983137356
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT	21814	41.237853983137356	No Hit
TCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG	2516	4.756323490491134	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC	1476	2.7902756247873266	No Hit
ATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCTT	812	1.5350296797610496	RNA PCR Primer, Index 42 (95% over 22bp)
TCTTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC	709	1.3403153238307688	No Hit
CTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTC	616	1.1645052743014859	RNA PCR Primer, Index 32 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACATCACTCGACCTCGTATGCCGT	485	0.9168588604484101	No Hit
CTTTACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTC	371	0.7013497674770313	No Hit
CTTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG	246	0.46504593746455447	No Hit
TCTTAATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC	236	0.44614163106355625	No Hit
TTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTC	193	0.36485311353926425	RNA PCR Primer, Index 32 (95% over 21bp)
CTTAATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG	180	0.3402775152179667	No Hit
TATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCT	179	0.3383870845778668	RNA PCR Primer, Index 42 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACATCACTCGAACTCGTATGCCGT	164	0.3100306249763696	No Hit
TCTTATACACATCTCCGAGCCCACGAGACATCACTCGACCTCGTATGCCG	162	0.30624976369617	No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGAGCTCGTATGCCGT	135	0.255208136413475	No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATATCGTATGCCGT	128	0.2419751219327763	No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACCCGATCTCGTATGCCGT	101	0.19093349465008128	No Hit
CCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG	80	0.15123445120798518	No Hit
TCTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT	79	0.14934402056788537	No Hit
CTTATACACATCTCCGAGCCCACGAGACATCCCTCGATCTCGTATGCCGT	62	0.11720669968618852	No Hit
CTCTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG	53	0.10019282392529018	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.007561722560399259	0.0
5	0.0	0.0	0.0	0.007561722560399259	0.0
6	0.0	0.0	0.0	0.007561722560399259	0.0
7	0.0	0.0	0.0	0.007561722560399259	0.0
8	0.0	0.0	0.0	0.009452153200499074	0.0
9	0.0	0.0	0.0	0.009452153200499074	0.0
10	0.0	0.0	0.0	0.013233014480698703	0.0
11	0.0	0.0	0.0	0.013233014480698703	0.0
12	0.0	0.0	0.0	0.013233014480698703	0.0
13	0.0	0.0	0.0	0.022685167681197778	0.0
14	0.0	0.0	0.0	0.022685167681197778	0.0
15	0.0	0.0	0.0	0.02835645960149722	0.0
16	0.0	0.0	0.0	0.03402775152179666	0.0
17	0.0	0.0	0.0	0.03402775152179666	0.0
18	0.0	0.0018904306400998148	0.0	0.039699043442096106	0.0
19	0.0	0.0018904306400998148	0.0	0.04347990472229574	0.0
20	0.0	0.0018904306400998148	0.0	0.04347990472229574	0.0
21	0.0	0.0018904306400998148	0.0	0.04726076600249537	0.0
22	0.0	0.0018904306400998148	0.0	0.05860334984309426	0.0
23	0.0	0.0018904306400998148	0.0	0.07183636432379296	0.0
24	0.0	0.0018904306400998148	0.0	0.07183636432379296	0.0
25	0.0	0.0018904306400998148	0.0	0.08506937880449167	0.0
26	0.0	0.0018904306400998148	0.0	0.09263110136489092	0.0
27	0.0	0.0018904306400998148	0.0	0.1682483269688835	0.0
28	0.0	0.0018904306400998148	0.0	0.20794737041097963	0.0
29	0.0	0.0018904306400998148	0.0	0.31381148625656924	0.0
30	0.0	0.0018904306400998148	0.0	0.39131914250066163	0.0
31	0.0	0.0018904306400998148	0.0	0.4763885213051533	0.0
32	0.0	0.0018904306400998148	0.0	0.5784717758705433	0.0
33	0.0	0.0018904306400998148	0.0	0.7070210593973307	0.0
34	0.0	0.0018904306400998148	0.0	0.9452153200499074	0.0
35	0.0	0.0018904306400998148	0.0	1.2268894854247798	0.0
36	0.0	0.0018904306400998148	0.0	1.7807856629740255	0.0
37	0.0	0.0018904306400998148	0.0	2.5275057658134523	0.0
38	0.0	0.0018904306400998148	0.0	3.3044727588944762	0.0
39	0.0	0.0018904306400998148	0.0	4.056864153654202	0.0
40	0.0	0.0018904306400998148	0.0	4.813036409694129	0.0
41	0.0	0.0018904306400998148	0.0	5.427426367726568	0.0
42	0.0	0.0018904306400998148	0.0	6.034254603198609	0.0
43	0.0	0.0018904306400998148	0.0	6.667548867632046	0.0
44	0.0	0.0018904306400998148	0.0	7.367008204468978	0.0
45	0.0	0.0018904306400998148	0.0	7.996521607622216	0.0
46	0.0	0.0018904306400998148	0.0	8.643048886536352	0.0
47	0.0	0.0018904306400998148	0.0	9.212068509206397	0.0
48	0.0	0.0018904306400998148	0.0	9.714923059472948	0.0
49	0.0	0.0018904306400998148	0.0	10.178078566297403	0.0
50	0.0	0.0018904306400998148	0.0	10.626110628001058	0.0
51	0.0	0.0018904306400998148	0.0	11.12896517826761	0.0
52	0.0	0.0018904306400998148	0.0	11.493818291806873	0.0
53	0.0	0.0018904306400998148	0.0	11.870013989186736	0.0
54	0.0	0.0018904306400998148	0.0	12.180044614163107	0.0
55	0.0	0.0018904306400998148	0.0	12.609172369465764	0.0
56	0.0	0.0018904306400998148	0.0	12.96268289916443	0.0
57	0.0	0.0018904306400998148	0.0	13.323755151423494	0.0
58	0.0	0.0018904306400998148	0.0	13.713183863284057	0.0
59	0.0	0.0018904306400998148	0.0	14.038337933381225	0.0
60	0.0	0.0018904306400998148	0.0	14.323792960036297	0.0
61	0.0	0.0018904306400998148	0.0	14.662180044614162	0.0
62	0.0	0.0018904306400998148	0.0	14.964648947030133	0.0
63	0.0	0.0018904306400998148	0.0	15.227418806004009	0.0
64	0.0	0.0018904306400998148	0.0	15.51476426329918	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGATATC	20	7.858949E-5	70.0	35
AATCTAA	20	7.858949E-5	70.0	70
TCGATAT	20	7.858949E-5	70.0	34
GATATCG	20	7.858949E-5	70.0	36
TATCGTA	20	7.858949E-5	70.0	38
GAAAAAC	15	0.0022131465	70.0	60
GCTCGTA	25	2.8036447E-6	70.0	38
AACAGAA	15	0.0022131465	70.0	70
TTTACAC	40	1.3096724E-10	70.0	2
ATATCGT	20	7.858949E-5	70.0	37
AATAAAC	15	0.0022131465	70.0	70
TCTTAAT	30	1.0054828E-7	70.0	1
CTCGATA	20	7.858949E-5	70.0	33
TCTTTAT	90	0.0	66.11111	1
ATCGTAT	35	2.921588E-7	60.0	39
AAACAGA	30	8.270461E-6	58.333332	69
TTTATAC	110	0.0	57.272724	3
CTTTATA	105	0.0	56.66667	2
AAATCTA	25	2.3701537E-4	55.999996	69
AACAAAT	20	0.0069155786	52.5	70
>>END_MODULE