FastQCFastQC Report
Wed 25 May 2016
SRR1779725_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779725_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51981
Sequences flagged as poor quality0
Sequence length76
%GC52

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTAGAC150.002212870.0009737
ATCTAGG252.8027025E-670.0009742
GCTGGTT150.002212870.0009747
TTGTCGC252.8027025E-670.0009749
CCGTGGT252.8027025E-670.0009746
TAACGTG150.002212870.0009732
GCGTTTA150.002212870.0009734
TAGCCGT150.002212870.0009752
CATTACA252.8027025E-670.0009761
TATTGGT150.002212870.0009743
GTGTGGA252.8027025E-670.0009736
TTAAGGT252.8027025E-670.0009743
GTGTATC150.002212870.0009736
AGATTTC150.002212870.0009740
CTCTGTG252.8027025E-670.0009744
TCATAAA150.002212870.0009764
TAGTGTA252.8027025E-670.0009734
ATTTGTA150.002212870.0009734
GCGTCAG150.002212870.0009748
TACCGCT150.002212870.0009713