##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779725_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	51981
Sequences flagged as poor quality	0
Sequence length	76
%GC	52
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	21.861256997749177	23.0	12.0	32.0	12.0	34.0
2	22.698851503433946	23.0	12.0	32.0	12.0	34.0
3	22.64213847367307	23.0	11.0	32.0	11.0	34.0
4	22.037513706931378	23.0	12.0	32.0	11.0	34.0
5	22.535041649833595	23.0	11.0	32.0	11.0	34.0
6	23.10045978338239	24.0	11.0	34.0	11.0	37.0
7	24.180238933456454	26.0	12.0	34.0	12.0	38.0
8	23.229853215598006	25.0	11.0	34.0	11.0	38.0
9	24.930493834285603	26.0	12.0	35.0	12.0	38.0
10-11	23.862190030972855	26.0	11.0	34.0	11.0	38.0
12-13	24.400819530212964	26.5	11.0	35.0	11.0	38.0
14-15	22.996633385275388	23.5	11.0	34.0	11.0	37.5
16-17	23.219868798214733	24.5	11.0	33.5	11.0	37.5
18-19	23.60686597025836	25.5	11.5	34.0	11.0	38.0
20-21	22.83044766356938	23.0	11.0	33.5	11.0	37.0
22-23	23.070208345356956	24.5	11.0	32.5	11.0	37.5
24-25	22.90102152709644	23.0	11.0	34.0	11.0	37.5
26-27	22.28077566803255	22.5	11.0	32.5	10.0	37.0
28-29	22.66440622535157	22.5	11.0	34.0	11.0	38.0
30-31	23.07925011061734	23.0	11.0	34.0	11.0	38.0
32-33	23.30119659106212	23.0	11.0	34.0	11.0	38.0
34-35	23.393249456532196	23.5	11.0	34.0	11.0	38.0
36-37	23.061435909274543	23.5	11.0	34.0	11.0	37.0
38-39	23.286094919297437	24.5	11.0	33.5	11.0	37.0
40-41	22.251688116811913	22.5	11.0	33.0	11.0	37.0
42-43	22.640493641907618	23.0	11.0	33.5	11.0	37.5
44-45	23.39239337450222	25.0	11.0	34.0	11.0	38.0
46-47	23.08619495584925	24.0	11.0	34.0	11.0	37.5
48-49	22.360785671687733	22.5	11.0	33.5	11.0	37.0
50-51	21.980685250379945	22.5	11.0	31.0	11.0	37.0
52-53	22.311546526615494	22.5	11.0	33.5	11.0	37.5
54-55	22.298945768646234	23.0	11.0	32.5	11.0	37.5
56-57	22.564167676651085	22.5	11.0	33.5	11.0	37.5
58-59	22.694397953098246	23.0	11.0	34.0	11.0	38.0
60-61	23.25798849579654	23.0	11.0	34.0	11.0	38.0
62-63	23.332698485985265	23.5	11.0	34.0	11.0	38.0
64-65	23.259710278755698	23.5	11.0	34.0	11.0	38.0
66-67	22.825878686443126	23.5	11.0	34.0	11.0	37.5
68-69	22.494382562859506	23.0	11.0	34.0	11.0	37.0
70-71	22.48826494295993	23.0	11.0	33.5	11.0	37.0
72-73	22.314576479867647	23.0	11.0	31.5	11.0	37.0
74-75	21.934331775071662	21.5	11.0	32.5	11.0	37.0
76	21.615032415690347	21.0	11.0	32.0	11.0	37.0
>>END_MODULE
>>Per sequence quality scores	fail
#Quality	Count
3	1.0
4	2.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	18.0
12	77.0
13	394.0
14	1040.0
15	1889.0
16	3048.0
17	4584.0
18	5838.0
19	6196.0
20	5159.0
21	3733.0
22	2705.0
23	1780.0
24	1359.0
25	989.0
26	826.0
27	749.0
28	770.0
29	774.0
30	812.0
31	895.0
32	1005.0
33	1247.0
34	1576.0
35	1902.0
36	1748.0
37	865.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.804851772763126	15.771147149920164	18.02196956580289	42.40203151151382
2	12.06979473269079	13.803120370904754	52.321040380138896	21.80604451626556
3	12.037090475366	13.379888805525095	52.563436640310876	22.019584078798022
4	11.810084453935092	25.75941209288009	25.343875646870973	37.08662780631384
5	11.277197437525249	20.31896269790885	47.86171870491141	20.54212115965449
6	17.898847655874263	36.09780496719955	17.731478809565033	28.271868567361153
7	17.537177045458918	19.984225005290394	19.199322829495394	43.2792751197553
8	14.740851898880294	35.13217130324368	18.79256608565162	31.334410712224404
9	14.715841317480471	14.912078186925237	19.110008080341682	51.262072415252604
10-11	16.961820752811256	22.396760198928405	30.973383225756802	29.668035822503537
12-13	15.647004501904652	14.859171187810228	37.00219323559968	32.49163107468544
14-15	23.470506752856977	22.245950209704105	22.61437531263227	31.669167724806652
16-17	29.247951056216092	15.780714917849858	21.786140290122745	33.1851937358113
18-19	16.063526876755553	15.389203124398785	32.83119781445996	35.7160721843857
20-21	29.58213894072606	15.080128513438119	23.15742896169607	32.18030358413975
22-23	31.552772326753626	13.74331447920274	21.2686136442341	33.435299549809535
24-25	17.229404748162686	23.711955057909115	20.625072145907883	38.43356804802032
26-27	28.145020104272877	22.19742588352989	21.745320225475673	27.912233786721565
28-29	19.883412212859287	21.10989264688907	24.689291623379123	34.317403516872524
30-31	18.33931336995104	21.613744144213474	26.42919668709178	33.61774579874371
32-33	20.626034091346337	19.019585209126937	26.513140174689294	33.84124052483743
34-35	19.40225285454563	19.44361611050723	26.180055215137028	34.97407581981011
36-37	28.223479164261807	13.641348262726538	32.00681057370426	26.128361999307398
38-39	28.185963292162068	10.86613567278464	38.32198237715957	22.625918657893724
40-41	22.451060555047857	24.62604011351065	23.8410850848925	29.081814246548987
42-43	14.401423692944062	25.19647924582752	29.18762926266173	31.214467798566687
44-45	15.341253426963592	16.217594151315474	33.831946515319125	34.609205906401804
46-47	27.769440917311172	14.864942860440955	26.137019508253488	31.228596713994385
48-49	26.14567701719959	15.321866943706953	31.79710646812113	26.735349570972332
50-51	42.11260424983407	12.716796367728964	23.294246659676595	21.876352722760373
52-53	14.959357414265789	18.889904285508152	32.4255687557116	33.72516954451446
54-55	26.111046981415214	15.916349224671977	25.385740120820348	32.586863673092466
56-57	25.770270400261648	16.793481920409402	24.34179516530873	33.09445251402022
58-59	15.589137816597248	20.725877045316814	30.710774647209902	32.97421049087603
60-61	15.295079601750757	18.16170458371411	30.366985714972827	36.17623009956231
62-63	15.120820347069913	21.56489283927816	29.589441686867524	33.72484512678441
64-65	15.253422087978683	23.04318131535154	28.74842482949681	32.954971767172964
66-67	12.793997402722331	35.63945938146313	18.98994757346929	32.57659564234525
68-69	13.911554152875066	33.305750134708646	19.52120698945424	33.26148872296205
70-71	12.942975874408402	34.749317018738694	17.968178844895917	34.33952826195698
72-73	11.603947824079418	39.845703951671865	16.38481665319943	32.165531571049286
74-75	14.892839278156142	26.928700604101735	21.80153141713802	36.3769287006041
76	14.710069644849744	24.433414136750166	23.61576051406364	37.24075570433645
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	1.0
6	1.0
7	0.0
8	0.0
9	0.5
10	0.5
11	0.0
12	0.0
13	0.0
14	0.5
15	0.5
16	1.0
17	2.0
18	2.0
19	4.0
20	10.5
21	15.0
22	24.0
23	34.0
24	54.5
25	74.0
26	85.0
27	112.0
28	159.5
29	191.0
30	244.0
31	338.0
32	418.5
33	458.0
34	564.5
35	763.5
36	961.5
37	1067.0
38	1163.5
39	1371.5
40	1619.0
41	1838.5
42	1922.0
43	2030.0
44	2262.0
45	2426.0
46	2466.0
47	2485.0
48	2480.0
49	2410.5
50	2365.0
51	2328.5
52	2220.0
53	2126.0
54	2104.0
55	1997.0
56	1837.5
57	1764.0
58	1743.0
59	1698.5
60	1582.5
61	1457.0
62	1403.0
63	1398.0
64	1367.0
65	1253.0
66	1140.0
67	1115.0
68	1053.0
69	902.5
70	790.0
71	766.0
72	703.5
73	607.5
74	505.0
75	436.0
76	392.5
77	324.5
78	248.5
79	197.0
80	155.0
81	99.5
82	66.0
83	46.0
84	41.5
85	27.0
86	15.5
87	14.0
88	9.5
89	3.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.005771339527904426
9	0.005771339527904426
10-11	0.004809449606587022
12-13	0.005771339527904426
14-15	0.005771339527904426
16-17	0.005771339527904426
18-19	0.005771339527904426
20-21	0.003847559685269618
22-23	0.005771339527904426
24-25	0.005771339527904426
26-27	0.003847559685269618
28-29	0.005771339527904426
30-31	0.004809449606587022
32-33	0.005771339527904426
34-35	0.004809449606587022
36-37	0.005771339527904426
38-39	0.005771339527904426
40-41	0.006733229449221832
42-43	0.006733229449221832
44-45	0.006733229449221832
46-47	0.005771339527904426
48-49	0.005771339527904426
50-51	0.004809449606587022
52-53	0.006733229449221832
54-55	0.005771339527904426
56-57	0.004809449606587022
58-59	0.004809449606587022
60-61	0.006733229449221832
62-63	0.005771339527904426
64-65	0.004809449606587022
66-67	0.006733229449221832
68-69	0.03270425732479175
70-71	0.005771339527904426
72-73	0.005771339527904426
74-75	0.005771339527904426
76	0.005771339527904426
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	51981.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	99.55945441603663
#Duplication Level	Percentage of deduplicated	Percentage of total
1	99.61740609058587	99.17854600719494
2	0.33235430514762715	0.6617802658663743
3	0.04057814190755912	0.12119813008599296
4	0.009661462358942649	0.03847559685269618
5	0.0	0.0
6	0.0	0.0
7	0.0	0.0
8	0.0	0.0
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.003847559685269618	0.0
5	0.0	0.0	0.0	0.007695119370539236	0.0
6	0.0	0.0	0.0	0.007695119370539236	0.0
7	0.0	0.0	0.0	0.007695119370539236	0.0
8	0.0	0.0	0.0	0.007695119370539236	0.0
9	0.0	0.0	0.0	0.009618899213174045	0.0
10	0.0	0.0	0.0	0.011542679055808852	0.0
11	0.0	0.0	0.0	0.01923779842634809	0.0
12	0.0	0.0	0.0	0.01923779842634809	0.0
13	0.0	0.0	0.0	0.01923779842634809	0.0
14	0.0	0.0	0.0	0.023085358111617705	0.0
15	0.0	0.0	0.0	0.025009137954252515	0.0
16	0.0	0.0	0.0	0.025009137954252515	0.0
17	0.0	0.0	0.0	0.025009137954252515	0.0
18	0.0	0.0	0.0	0.026932917796887324	0.0
19	0.0	0.0	0.0	0.03270425732479175	0.0
20	0.0	0.0	0.0	0.03270425732479175	0.0
21	0.0	0.0	0.0	0.03462803716742656	0.0
22	0.0	0.0	0.0	0.06925607433485312	0.0
23	0.0	0.0	0.0	0.06925607433485312	0.0
24	0.0	0.0	0.0	0.0865700929185664	0.0
25	0.0	0.0	0.0	0.09041765260383601	0.0
26	0.0	0.0	0.0	0.09618899213174044	0.0
27	0.0	0.0	0.0	0.1327408091418018	0.0
28	0.0	0.0	0.0	0.14813104788288028	0.0
29	0.0	0.0	0.0	0.17891152536503724	0.0
30	0.0	0.0	0.0	0.20776822300455935	0.0
31	0.0	0.0	0.0	0.22700602143090745	0.0
32	0.0	0.0	0.0	0.2981858756083954	0.0
33	0.0	0.0	0.0	0.35397549104480486	0.0
34	0.0	0.0	0.0	0.4174602258517535	0.0
35	0.0	0.0	0.0	0.5309632365672072	0.0
36	0.0	0.0	0.0	0.6290760085415825	0.0
37	0.0	0.0	0.0	0.8310728920182374	0.0
38	0.0	0.0	0.0	1.0523075739212404	0.0
39	0.0	0.0	0.0	1.2831611550374176	0.0
40	0.0	0.0	0.0	1.5371000942652122	0.0
41	0.0	0.0	0.0	1.7948865931782767	0.0
42	0.0	0.0	0.0	1.994959696812297	0.0
43	0.0	0.0	0.0	2.237355956984283	0.0
44	0.0	0.0	0.0	2.4566668590446508	0.0
45	0.0	0.0	0.0	2.5624747503895655	0.0
46	0.0	0.0	0.0	2.7548527346530465	0.0
47	0.0	0.0	0.0	2.96839229718551	0.0
48	0.0	0.0	0.0	3.1145995652257557	0.0
49	0.0	0.0	0.0	3.2781208518497142	0.0
50	0.0	0.0	0.0	3.4955079740674475	0.0
51	0.0	0.0	0.0	3.685962178488294	0.0
52	0.0	0.0	0.0	3.8744926030665052	0.0
53	0.0	0.0	0.0	4.074565706700525	0.0
54	0.0	0.0	0.0	4.261172351436102	0.0
55	0.0	0.0	0.0	4.418922298532156	0.0
56	0.0	0.0	0.0	4.561282006887132	0.0
57	0.0	0.0	0.0	4.657470999018872	0.0
58	0.0	0.0	0.0	4.772897789576961	0.0
59	0.0	0.0	0.0	4.909486158404032	0.0
60	0.0	0.0	0.0	5.013370269906312	0.0
61	0.0	0.0	0.0	5.1057117023527825	0.0
62	0.0	0.0	0.0	5.173043996845001	0.0
63	0.0	0.0	0.0	5.273080548662011	0.0
64	0.0	0.0	0.0	5.348107962524769	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTAGAC	15	0.0022128	70.00097	37
ATCTAGG	25	2.8027025E-6	70.00097	42
GCTGGTT	15	0.0022128	70.00097	47
TTGTCGC	25	2.8027025E-6	70.00097	49
CCGTGGT	25	2.8027025E-6	70.00097	46
TAACGTG	15	0.0022128	70.00097	32
GCGTTTA	15	0.0022128	70.00097	34
TAGCCGT	15	0.0022128	70.00097	52
CATTACA	25	2.8027025E-6	70.00097	61
TATTGGT	15	0.0022128	70.00097	43
GTGTGGA	25	2.8027025E-6	70.00097	36
TTAAGGT	25	2.8027025E-6	70.00097	43
GTGTATC	15	0.0022128	70.00097	36
AGATTTC	15	0.0022128	70.00097	40
CTCTGTG	25	2.8027025E-6	70.00097	44
TCATAAA	15	0.0022128	70.00097	64
TAGTGTA	25	2.8027025E-6	70.00097	34
ATTTGTA	15	0.0022128	70.00097	34
GCGTCAG	15	0.0022128	70.00097	48
TACCGCT	15	0.0022128	70.00097	13
>>END_MODULE