FastQCFastQC Report
Wed 25 May 2016
SRR1779725_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779725_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51981
Sequences flagged as poor quality0
Sequence length76
%GC45

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT2551349.08139512514188No Hit
TCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG30665.898308997518324No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC18273.5147457724937956No Hit
ATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCTT11022.1200053865835593RNA PCR Primer, Index 15 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC7761.4928531578846116No Hit
CTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTC6771.3023989534637657No Hit
CTTTACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTC4310.8291491121756027No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAACCTCGTATGCCGT4050.7791308362670976No Hit
CTTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG2820.5425059156230161No Hit
TTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTC2660.5117254381408591No Hit
CTTAATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG2630.5059540986129547No Hit
TCTTAATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC2320.4463169234912756No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTATCTCGTATGCCGTC2210.42515534522229276No Hit
TATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCT2080.40014620726804023No Hit
TCTTATACACATCTCCGAGCCCACGAGACATAAGTTAACCTCGTATGCCG1430.27510051749677766No Hit
TCTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT1080.20776822300455935No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAAACTCGTATGCCGT1080.20776822300455935No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAAGCTCGTATGCCGT980.1885304245782113No Hit
CCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG890.171216405994498No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATATCGTATGCCGT800.1539023874107847No Hit
CTTATATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC560.10773167118754931No Hit
CTTATTACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG550.1058078913449145No Hit
CTTATACACATCTCCGAGCCCACGAGACATACGTTAATCTCGTATGCCGT550.1058078913449145No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTAA150.00221293370.0000131
AATCAAA150.00221293370.0000169
TACGTTA150.00221293370.0000130
AAAATGA150.00221293370.0000167
TTGAACA150.00221293370.0000158
AGACTTA150.00221293370.0000125
CGAGACT150.00221293370.0000123
CTTGAAC150.00221293370.0000157
ATCAAAA150.00221293370.0000170
AAAGCCT150.00221293370.0000169
TGAACAA150.00221293370.0000159
GAGACTT150.00221293370.0000124
AACATCC150.00221293370.0000170
GACATTA207.8576886E-570.026
ACATTAG207.8576886E-570.027
TCTTTAT850.070.01
AGACATT207.8576886E-570.025
TCTTAAT353.621608E-970.01
CTTTACA501.2732926E-1162.9999961
TTTACAC501.2732926E-1162.9999962
Produced by FastQC (version 0.11.5)