##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779725_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	51981
Sequences flagged as poor quality	0
Sequence length	76
%GC	45
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.667840172370674	34.0	34.0	34.0	34.0	34.0
2	33.66678209345722	34.0	34.0	34.0	34.0	34.0
3	33.69438833419903	34.0	34.0	34.0	34.0	34.0
4	33.70679671418403	34.0	34.0	34.0	34.0	34.0
5	33.756218618341315	34.0	34.0	34.0	34.0	34.0
6	37.45930243742906	38.0	38.0	38.0	37.0	38.0
7	37.43271580000385	38.0	38.0	38.0	37.0	38.0
8	37.33629595429099	38.0	38.0	38.0	37.0	38.0
9	37.3888728573902	38.0	38.0	38.0	37.0	38.0
10-11	37.25680537119332	38.0	38.0	38.0	36.0	38.0
12-13	37.25750755083588	38.0	38.0	38.0	36.0	38.0
14-15	37.26950231815471	38.0	38.0	38.0	36.0	38.0
16-17	37.077403281968415	38.0	38.0	38.0	35.5	38.0
18-19	37.03545526249976	38.0	38.0	38.0	35.0	38.0
20-21	37.16697447144149	38.0	38.0	38.0	36.0	38.0
22-23	37.19529251072507	38.0	38.0	38.0	36.0	38.0
24-25	37.074777322483214	38.0	38.0	38.0	35.0	38.0
26-27	36.84523191166003	38.0	38.0	38.0	34.5	38.0
28-29	36.76062407418095	38.0	37.5	38.0	34.5	38.0
30-31	36.51337026990631	38.0	37.0	38.0	34.5	38.0
32-33	36.28193955483735	38.0	37.0	38.0	34.0	38.0
34-35	36.18742425116869	38.0	37.0	38.0	34.0	38.0
36-37	36.12802754852735	38.0	37.0	38.0	33.0	38.0
38-39	34.169446528539275	37.0	34.0	38.0	25.5	38.0
40-41	36.04113041303553	37.5	36.5	38.0	33.0	38.0
42-43	36.829043304284255	38.0	37.0	38.0	34.5	38.0
44-45	36.840961120409375	38.0	37.5	38.0	34.5	38.0
46-47	36.83774840807218	38.0	37.5	38.0	34.5	38.0
48-49	36.739462495911965	38.0	37.0	38.0	34.5	38.0
50-51	36.51135991997076	38.0	37.0	38.0	34.0	38.0
52-53	36.56583174621497	38.0	37.0	38.0	34.0	38.0
54-55	36.55616475250572	38.0	37.0	38.0	34.0	38.0
56-57	36.57624901406283	38.0	37.0	38.0	34.0	38.0
58-59	36.3451357226679	38.0	37.0	38.0	34.0	38.0
60-61	36.305929089475	38.0	37.0	38.0	34.0	38.0
62-63	36.48856312883554	38.0	37.0	38.0	34.0	38.0
64-65	36.57733594967392	38.0	37.0	38.0	34.0	38.0
66-67	36.32782170408419	38.0	37.0	38.0	34.0	38.0
68-69	35.67484273099787	38.0	36.5	38.0	31.0	38.0
70-71	31.067014870818184	34.5	28.5	38.0	17.5	38.0
72-73	23.367605471229872	23.0	11.0	35.5	10.0	38.0
74-75	22.397876147053733	22.0	11.0	34.0	10.5	38.0
76	22.397741482464745	22.0	11.0	34.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	0.0
21	2.0
22	7.0
23	9.0
24	27.0
25	45.0
26	108.0
27	185.0
28	347.0
29	560.0
30	858.0
31	1363.0
32	2019.0
33	3131.0
34	5916.0
35	15305.0
36	13446.0
37	8652.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	12.508416536811529	5.9098516765741325	12.62191954752698	68.95981223908736
2	7.031415324830226	4.699794155556837	70.7585463919509	17.51024412766203
3	7.146842115388314	8.174140551355302	70.77970797021989	13.899309363036494
4	5.7828822069602355	57.88268790519613	22.19464804447779	14.139781843365846
5	6.256132048248399	20.713337565648988	65.99334372174448	7.0371866643581305
6	10.921298166637811	65.98180104268867	11.494584559742984	11.602316230930532
7	9.965179584848311	18.876127815932744	9.784344279640637	61.37434831957831
8	8.691637329024067	66.90521536715337	6.246513149035224	18.156634154787326
9	7.358457898078144	14.728458475212097	4.382370481522094	73.53071314518766
10-11	9.54290990938997	38.51888189915546	33.905657836517186	18.03255035493738
12-13	9.75741136184375	6.915988534272138	40.040591754679596	43.28600834920452
14-15	11.121371270271831	5.19228179527135	11.257959639098901	72.42838729535792
16-17	41.51324522421654	32.77928473865451	4.075527596621843	21.631942440507107
18-19	42.83392008618534	12.51514976626075	3.9918431734672284	40.659086974086684
20-21	12.920105423135375	6.209961332025163	4.063984917566034	76.80594832727343
22-23	11.658105846366942	36.56047401935322	4.069756257093938	47.71166387718589
24-25	41.64694792327966	36.938496758430965	3.9687578153556107	17.445797502933765
26-27	42.72234085531252	41.429560801061925	5.327908274177103	10.520190069448452
28-29	12.20349743175391	42.44146899828783	6.055097054693061	39.299936515265195
30-31	10.121005752101729	41.44110348011773	36.4094573017064	12.028433466074143
32-33	36.51430330312999	43.739058502145014	10.927069506165713	8.819568688559281
34-35	16.047209557338256	10.683711356072411	64.53223293126335	8.736846155325985
36-37	8.627190704295801	62.943190781247	18.172024393528403	10.2575941209288
38-39	9.386121852215233	14.731344144976049	36.93176352898174	38.95077047382697
40-41	9.944979896500644	5.472191762374714	40.46189954021662	44.12092880090802
42-43	39.82320463246186	5.78480598680287	38.36497951174467	16.027009868990593
44-45	12.460322040745657	34.79636790365711	41.35934283680576	11.383967218791481
46-47	39.50193339874185	7.45272311036725	14.21192358746465	38.833419903426254
48-49	38.73434524153056	4.478559473653835	8.750312614224429	48.03678267059118
50-51	14.119582155018179	4.713260614455281	39.84532810065216	41.321829129874374
52-53	8.11546526615494	4.785402358554087	69.18681826051827	17.912314114772705
54-55	8.91287201092707	4.551663107673957	45.40793751563071	41.127527365768266
56-57	38.795906196494876	4.676708797445221	16.241511321444374	40.28587368461553
58-59	12.339123910659664	7.279582924530118	64.21384736730728	16.167445797502932
60-61	37.880186991400706	37.61085781343183	15.308478097766493	9.200477097400974
62-63	10.088301494776939	75.91908581981878	4.863315442180797	9.129297243223485
64-65	6.97081625978723	79.34822338931534	4.534349089090244	9.146611261807198
66-67	6.939073892383756	79.38092764664012	4.535310979011562	9.144687481964564
68-69	7.043919893807353	78.95769608126047	4.783478578711453	9.214905446220735
70-71	7.575845020295878	72.96127431176777	6.839999230488063	12.6228814374483
72-73	9.607356534118235	57.77880379369385	12.153479155845405	20.46036051634251
74-75	10.829918624112658	50.16736884630922	15.623016102037282	23.379696427540832
76	10.201804505492392	50.870510378792254	15.313287547373077	23.61439756834228
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	1.5
23	3.5
24	5.0
25	6.0
26	8.0
27	8.5
28	11.5
29	16.0
30	17.5
31	22.5
32	24.5
33	23.0
34	34.5
35	235.0
36	5596.5
37	10769.0
38	11282.0
39	10161.0
40	6539.0
41	3350.5
42	2150.0
43	1483.0
44	587.5
45	277.0
46	195.0
47	182.5
48	159.5
49	149.0
50	149.0
51	151.0
52	184.5
53	238.5
54	261.0
55	303.0
56	360.0
57	454.0
58	533.0
59	512.0
60	556.5
61	593.0
62	564.0
63	623.0
64	741.0
65	723.0
66	672.5
67	699.0
68	719.5
69	662.5
70	595.0
71	605.0
72	638.5
73	636.5
74	558.5
75	516.0
76	490.5
77	391.5
78	300.0
79	282.0
80	245.5
81	176.5
82	121.5
83	99.0
84	77.0
85	48.0
86	35.0
87	29.0
88	21.5
89	8.0
90	2.5
91	1.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	51981.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.071987841711394
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.79667873110496	11.710047902118081
2	20.76857568660847	7.506588945961024
3	8.377687885884608	4.542044208460784
4	3.2999787098147757	2.385487004867163
5	1.0645092612305727	0.9618899213174045
6	0.468384074941452	0.5078778784555895
7	0.18096657440919736	0.22892980127354223
8	0.08516074089844582	0.12312190992862777
9	0.07451564828614009	0.12119813008599296
>10	0.6387055567383436	2.539389392277948
>50	0.06387055567383436	0.8329966718608722
>100	0.117096018735363	5.130720840307036
>500	0.021290185224611454	2.7952521113483773
>1k	0.03193527783691718	11.53306015659568
>5k	0.0	0.0
>10k+	0.010645092612305727	49.08139512514188
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT	25513	49.08139512514188	No Hit
TCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG	3066	5.898308997518324	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC	1827	3.5147457724937956	No Hit
ATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCTT	1102	2.1200053865835593	RNA PCR Primer, Index 15 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC	776	1.4928531578846116	No Hit
CTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTC	677	1.3023989534637657	No Hit
CTTTACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTC	431	0.8291491121756027	No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAACCTCGTATGCCGT	405	0.7791308362670976	No Hit
CTTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG	282	0.5425059156230161	No Hit
TTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTC	266	0.5117254381408591	No Hit
CTTAATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG	263	0.5059540986129547	No Hit
TCTTAATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC	232	0.4463169234912756	No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTATCTCGTATGCCGTC	221	0.42515534522229276	No Hit
TATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCT	208	0.40014620726804023	No Hit
TCTTATACACATCTCCGAGCCCACGAGACATAAGTTAACCTCGTATGCCG	143	0.27510051749677766	No Hit
TCTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT	108	0.20776822300455935	No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAAACTCGTATGCCGT	108	0.20776822300455935	No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAAGCTCGTATGCCGT	98	0.1885304245782113	No Hit
CCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG	89	0.171216405994498	No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATATCGTATGCCGT	80	0.1539023874107847	No Hit
CTTATATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC	56	0.10773167118754931	No Hit
CTTATTACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG	55	0.1058078913449145	No Hit
CTTATACACATCTCCGAGCCCACGAGACATACGTTAATCTCGTATGCCGT	55	0.1058078913449145	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.003847559685269618	0.0
5	0.0	0.0	0.0	0.013466458898443662	0.0
6	0.0	0.0	0.0	0.013466458898443662	0.0
7	0.0	0.0	0.0	0.013466458898443662	0.0
8	0.0	0.0	0.0	0.013466458898443662	0.0
9	0.0	0.0	0.0	0.01923779842634809	0.0
10	0.0	0.0	0.0	0.0211615782689829	0.0
11	0.0	0.0	0.0	0.030780477482156943	0.0
12	0.0	0.0	0.0	0.030780477482156943	0.0
13	0.0	0.0	0.0	0.03270425732479175	0.0
14	0.0	0.0	0.0	0.04039937669533099	0.0
15	0.0	0.0	0.0	0.0423231565379658	0.0
16	0.0	0.0	0.0	0.0423231565379658	0.0
17	0.0	0.0	0.0	0.04617071622323541	0.0
18	0.0	0.0	0.0	0.05194205575113984	0.0
19	0.0	0.0	0.0	0.06156095496431389	0.0
20	0.0	0.0	0.0	0.06733229449221831	0.0
21	0.0	0.0	0.0	0.06733229449221831	0.0
22	0.0	0.0	0.0	0.06733229449221831	0.0
23	0.0	0.0	0.0	0.06925607433485312	0.0
24	0.0	0.0	0.0	0.07117985417748793	0.0
25	0.0	0.0	0.0	0.07695119370539236	0.0
26	0.0	0.0	0.0	0.08272253323329679	0.0
27	0.0	0.0	0.0	0.12119813008599296	0.0
28	0.0	0.0	0.0	0.14235970835497586	0.0
29	0.0	0.0	0.0	0.20007310363402012	0.0
30	0.0	0.0	0.0	0.24432004001462074	0.0
31	0.0	0.0	0.0	0.29626209576576057	0.0
32	0.0	0.0	0.0	0.4097651064812143	0.0
33	0.0	0.0	0.0	0.532887016409842	0.0
34	0.0	0.0	0.0	0.6733229449221831	0.0
35	0.0	0.0	0.0	0.8637771493430292	0.0
36	0.0	0.0	0.0	1.136953886997172	0.0
37	0.0	0.0	0.0	1.4832342586714375	0.0
38	0.0	0.0	0.0	1.8910755853100172	0.0
39	0.0	0.0	0.0	2.2335083972990133	0.0
40	0.0	0.0	0.0	2.66443508204921	0.0
41	0.0	0.0	0.0	3.010715453723476	0.0
42	0.0	0.0	0.0	3.314672668859776	0.0
43	0.0	0.0	0.0	3.6513341413208673	0.0
44	0.0	0.0	0.0	3.978376714568785	0.0
45	0.0	0.0	0.0	4.180373598045439	0.0
46	0.0	0.0	0.0	4.48048325349647	0.0
47	0.0	0.0	0.0	4.767126450049056	0.0
48	0.0	0.0	0.0	5.005675150535772	0.0
49	0.0	0.0	0.0	5.300013466458898	0.0
50	0.0	0.0	0.0	5.5424097266308845	0.0
51	0.0	0.0	0.0	5.763644408533888	0.0
52	0.0	0.0	0.0	5.982955310594256	0.0
53	0.0	0.0	0.0	6.173409515015102	0.0
54	0.0	0.0	0.0	6.3638637194359475	0.0
55	0.0	0.0	0.0	6.552394144014159	0.0
56	0.0	0.0	0.0	6.739000788749736	0.0
57	0.0	0.0	0.0	6.89290317616052	0.0
58	0.0	0.0	0.0	7.046805563571305	0.0
59	0.0	0.0	0.0	7.235335988149516	0.0
60	0.0	0.0	0.0	7.402704834458745	0.0
61	0.0	0.0	0.0	7.602777938092765	0.0
62	0.0	0.0	0.0	7.7662992247167235	0.0
63	0.0	0.0	0.0	7.949058309767031	0.0
64	0.0	0.0	0.0	8.10873203670572	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTTAA	15	0.002212933	70.00001	31
AATCAAA	15	0.002212933	70.00001	69
TACGTTA	15	0.002212933	70.00001	30
AAAATGA	15	0.002212933	70.00001	67
TTGAACA	15	0.002212933	70.00001	58
AGACTTA	15	0.002212933	70.00001	25
CGAGACT	15	0.002212933	70.00001	23
CTTGAAC	15	0.002212933	70.00001	57
ATCAAAA	15	0.002212933	70.00001	70
AAAGCCT	15	0.002212933	70.00001	69
TGAACAA	15	0.002212933	70.00001	59
GAGACTT	15	0.002212933	70.00001	24
AACATCC	15	0.002212933	70.00001	70
GACATTA	20	7.8576886E-5	70.0	26
ACATTAG	20	7.8576886E-5	70.0	27
TCTTTAT	85	0.0	70.0	1
AGACATT	20	7.8576886E-5	70.0	25
TCTTAAT	35	3.621608E-9	70.0	1
CTTTACA	50	1.2732926E-11	62.999996	1
TTTACAC	50	1.2732926E-11	62.999996	2
>>END_MODULE