FastQCFastQC Report
Wed 25 May 2016
SRR1779724_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779724_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences127703
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAT653.6379788E-1253.84404854
GTAGCGC200.00693903952.4979489
CCATAGG200.00693903952.49794813
GCTAGTC200.00693903952.49794821
GTATCAC200.00693903952.49794857
GGTCTAG200.00693903952.4979481
TATACGT200.00693903952.49794833
CCGCAAT200.00693903952.49794824
CGTATCA552.2610038E-950.90709756
GTAGCTC552.2610038E-950.90709738
TAGATCT1400.049.9980439
CGCTGTA305.860815E-446.6648453
TGTAGAT1500.046.6648437
AGATCTC1600.045.93570340
GTAGATC1450.045.86027538
TCTCGGT1450.045.86027543
CTCGGTG1300.045.7674444
TCGGTGG1150.045.65038345
ATCTCGG1550.042.90154642
CCGTGTA757.4578566E-1041.99835634
Produced by FastQC (version 0.11.5)