FastQCFastQC Report
Wed 25 May 2016
SRR1779724_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779724_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences127703
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT1797614.076411673962241No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG19671.5402927104296689No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC12871.0078071775917559No Hit
ATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTCTT7700.6029615592429309Illumina PCR Primer Index 9 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC5640.44164976547144547No Hit
CTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTC4970.3891842791477099No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGACCTCGTATGCCGT3820.29913157874129814No Hit
CTTTACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTC2940.2302216862563918No Hit
CTTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG1720.13468751712958976No Hit
TTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTC1620.1268568475290322No Hit
TATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTCT1530.11980924488853041No Hit
CTTAATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG1450.11354470920808438No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC1350.10571403960752683No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGACCTCGTATGCCG1310.10258177176730382No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATTTAC150.002220366270.036
GCTTTTG150.002220366270.044
ATTTACT200.00693796652.537
ACAGTCG200.00693796652.555
TGCATCC200.00693796652.533
CCGTATC200.00693796652.563
GGAACCG200.00693796652.55
GTATCGA200.00693796652.565
TAAGGGA200.00693796652.537
GACTGTT200.00693796652.549
CGACTAA200.00693796652.529
GATTATC200.00693796652.556
TCTTTAT1100.050.909091
TGGACAG352.0769676E-550.05
CCGTCTT25950.049.4990447
TCTTCTG26100.049.48275850
CTGCTTG26050.049.30902554
CTCGTAT26150.049.25430339
CTTGAAA25950.049.22928657
CGTCTTC26200.049.16030548
Produced by FastQC (version 0.11.5)