##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779724_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	127703
Sequences flagged as poor quality	0
Sequence length	76
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.67244309060868	34.0	34.0	34.0	34.0	34.0
2	33.67862931959312	34.0	34.0	34.0	34.0	34.0
3	33.69560621128713	34.0	34.0	34.0	34.0	34.0
4	33.71955239892563	34.0	34.0	34.0	34.0	34.0
5	33.71698393929665	34.0	34.0	34.0	34.0	34.0
6	37.39161961739348	38.0	38.0	38.0	37.0	38.0
7	37.38241858061283	38.0	38.0	38.0	37.0	38.0
8	37.27868570041424	38.0	38.0	38.0	36.0	38.0
9	37.28527129354831	38.0	38.0	38.0	36.0	38.0
10-11	37.20633031330509	38.0	38.0	38.0	36.0	38.0
12-13	37.1797765126896	38.0	38.0	38.0	36.0	38.0
14-15	37.20579391243746	38.0	38.0	38.0	36.0	38.0
16-17	37.167509768760326	38.0	38.0	38.0	36.0	38.0
18-19	37.16052089614183	38.0	38.0	38.0	36.0	38.0
20-21	37.179361487200765	38.0	38.0	38.0	36.0	38.0
22-23	37.14228326664213	38.0	38.0	38.0	36.0	38.0
24-25	37.115686397343836	38.0	38.0	38.0	36.0	38.0
26-27	37.04039059379967	38.0	38.0	38.0	35.5	38.0
28-29	37.01184780310564	38.0	38.0	38.0	35.5	38.0
30-31	36.912691949288586	38.0	38.0	38.0	35.5	38.0
32-33	36.891138031213046	38.0	38.0	38.0	35.0	38.0
34-35	36.78689224215562	38.0	37.0	38.0	34.5	38.0
36-37	36.85501906768047	38.0	37.0	38.0	35.0	38.0
38-39	36.128877160285974	38.0	37.0	38.0	34.0	38.0
40-41	36.719853879705255	38.0	37.0	38.0	34.5	38.0
42-43	36.96654737946642	38.0	37.5	38.0	35.0	38.0
44-45	36.97118705120475	38.0	38.0	38.0	35.0	38.0
46-47	36.98908404657682	38.0	38.0	38.0	35.5	38.0
48-49	36.969476049897025	38.0	38.0	38.0	35.5	38.0
50-51	36.918987807647426	38.0	38.0	38.0	35.0	38.0
52-53	36.884767781493	38.0	38.0	38.0	35.0	38.0
54-55	36.8918741141555	38.0	38.0	38.0	35.0	38.0
56-57	36.91115322271207	38.0	38.0	38.0	35.0	38.0
58-59	36.821413749089686	38.0	37.0	38.0	35.0	38.0
60-61	36.87129902978003	38.0	37.5	38.0	35.0	38.0
62-63	36.88821719145204	38.0	38.0	38.0	35.0	38.0
64-65	36.92459456708143	38.0	38.0	38.0	35.0	38.0
66-67	36.8469926313399	38.0	38.0	38.0	35.0	38.0
68-69	36.65422503778298	38.0	37.5	38.0	34.0	38.0
70-71	35.33457318935342	38.0	36.5	38.0	28.5	38.0
72-73	33.24607096152792	38.0	34.0	38.0	16.0	38.0
74-75	33.007540934825336	38.0	34.0	38.0	11.0	38.0
76	32.62115220472503	38.0	32.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	1.0
21	4.0
22	6.0
23	20.0
24	34.0
25	80.0
26	111.0
27	223.0
28	396.0
29	673.0
30	1065.0
31	1769.0
32	2723.0
33	4515.0
34	7714.0
35	16964.0
36	20852.0
37	70553.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.35153441970823	14.388855391024485	10.465689921145158	38.79392026812213
2	19.80376341981003	14.916642522102064	41.08360805932516	24.195985998762755
3	18.17498414289406	17.187536706263753	38.34913823481046	26.28834091603173
4	13.711502470576258	27.32433850418549	29.686851522673702	29.277307502564543
5	14.234591199893504	28.62892805963838	41.65681307408596	15.479667666382152
6	27.433967878593297	42.07497083075574	14.909594919461563	15.5814663711894
7	26.075346702896564	24.17092785604097	16.457718299491788	33.296007141570676
8	22.90392551467076	41.289554669819815	14.59480200151915	21.211717813990276
9	20.7457929727571	15.34184788141234	15.157044078839183	48.75531506699138
10-11	23.589500638199574	26.872900401713352	26.678308262139495	22.859290697947582
12-13	22.879258905429005	16.8469025786395	28.277722528053374	31.99611598787812
14-15	21.620478767139378	16.95731502000736	18.508570667877812	42.91363554497545
16-17	30.688394164585013	26.58355715997275	17.777577660665763	24.950471014776472
18-19	30.774531530191148	20.55550770146355	18.339819737985795	30.33014103035951
20-21	22.135736826856064	19.22429386936877	18.088846777287927	40.55112252648724
22-23	22.25672067218468	27.580401400123723	18.064963235006225	32.09791469268537
24-25	30.444468806527645	27.75502533221616	17.990571873800928	23.809933987455267
26-27	30.17626837270855	28.67317134288153	19.295161429253817	21.855398855156103
28-29	22.367916180512594	20.479550206338143	27.539681918200827	29.612851694948432
30-31	29.176291864717353	18.64130051760726	29.49969851922038	22.68270909845501
32-33	23.041353766160544	19.60603901239595	27.41321660415182	29.939390617291682
34-35	21.78805509659131	35.13229916290142	21.14985552414587	21.9297902163614
36-37	29.907284871929395	28.95820771634182	19.508938709349035	21.62556870237974
38-39	21.60716662881843	20.268905194083146	28.161828617965124	29.962099559133303
40-41	20.56725370586439	17.904042974714766	30.191146644949608	31.337556674471234
42-43	28.882641754696444	18.106857317369208	29.490301715699708	23.520199212234637
44-45	20.62794139526871	26.32318739575421	30.845007556596165	22.203863652380914
46-47	28.163786285365262	18.59666570088408	23.445416317549313	29.794131696201344
48-49	27.489565632757255	18.00858241388221	21.96111289476363	32.5407390585969
50-51	20.139699145673948	18.378973085988584	30.707579305106382	30.773748463231094
52-53	18.063397101086114	18.50113153175728	39.136903596626546	24.29856777053006
54-55	17.990963407280958	18.513269069638145	32.311691972780594	31.184075550300307
56-57	26.28051024643117	18.547724015880597	23.79818798305443	31.373577754633796
58-59	19.059066740797007	19.3370555116168	36.961152048111636	24.642725699474564
60-61	26.478626187325276	27.490740233197343	22.840497090906243	23.190136488571138
62-63	18.332380601865268	38.29667274848672	19.715668386803756	23.655278262844256
64-65	17.81359874082833	39.78175923823246	18.950220433349255	23.454421587589955
66-67	18.110772652169487	39.67095526338457	18.474507255115384	23.743764829330555
68-69	17.838265350070085	39.8581082668379	18.607628638324865	23.695997744767155
70-71	18.2822643164217	37.88360492705731	19.177701385245452	24.656429371275536
72-73	19.0140403905938	32.788971284934576	20.897707963007917	27.29928036146371
74-75	19.31121430193496	31.13552539877685	22.049207927769903	27.504052371518288
76	19.336272444656743	31.72909015449911	22.015927581967535	26.918709818876614
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	1.0
14	3.0
15	2.0
16	2.0
17	4.0
18	6.5
19	12.0
20	23.5
21	32.0
22	27.0
23	44.5
24	92.5
25	118.0
26	149.0
27	215.0
28	325.5
29	401.0
30	504.0
31	611.5
32	835.0
33	1054.0
34	1189.5
35	1500.0
36	1853.0
37	2031.0
38	2470.5
39	6730.0
40	11305.5
41	11117.5
42	10174.0
43	9128.0
44	7300.5
45	5993.0
46	5467.0
47	5237.5
48	4869.0
49	4544.5
50	4359.0
51	4115.0
52	3552.5
53	2945.5
54	2657.0
55	2632.5
56	2557.5
57	2430.5
58	2354.0
59	2333.0
60	2305.5
61	2290.5
62	2282.0
63	2274.0
64	2312.5
65	2312.0
66	2198.5
67	2132.0
68	2174.5
69	2061.0
70	1882.0
71	1859.0
72	1847.0
73	1749.0
74	1605.0
75	1547.0
76	1421.0
77	1194.5
78	1008.5
79	923.0
80	791.0
81	593.0
82	440.0
83	353.0
84	266.5
85	145.0
86	88.0
87	66.0
88	47.5
89	20.5
90	10.0
91	5.0
92	2.0
93	1.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	127703.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.93415972999851
#Duplication Level	Percentage of deduplicated	Percentage of total
1	49.59140534768681	17.820254809988803
2	24.17137004510885	17.371557441876856
3	12.0115931922683	12.948795251481954
4	5.8053128200658115	8.344361526354119
5	2.782801978687703	4.999882539955991
6	1.6016910370677069	3.453325293845877
7	1.0547189958377825	2.653030860668896
8	0.6537514437011048	1.8793607041338103
9	0.5055677831288544	1.635043812596415
>10	1.7782039268670051	9.2331425260174
>50	0.008716685916014731	0.1855868695332138
>100	0.02397088626904051	1.8065354768486253
>500	0.004358342958007366	1.0446113247143765
>1k	0.004358342958007366	2.5480998880214245
>5k	0.0	0.0
>10k+	0.002179171479003683	14.076411673962241
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT	17976	14.076411673962241	No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG	1967	1.5402927104296689	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC	1287	1.0078071775917559	No Hit
ATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTCTT	770	0.6029615592429309	Illumina PCR Primer Index 9 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC	564	0.44164976547144547	No Hit
CTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTC	497	0.3891842791477099	No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGACCTCGTATGCCGT	382	0.29913157874129814	No Hit
CTTTACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTC	294	0.2302216862563918	No Hit
CTTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG	172	0.13468751712958976	No Hit
TTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTC	162	0.1268568475290322	No Hit
TATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTCT	153	0.11980924488853041	No Hit
CTTAATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG	145	0.11354470920808438	No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC	135	0.10571403960752683	No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGACCTCGTATGCCG	131	0.10258177176730382	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	7.830669600557544E-4	0.0
4	0.0	0.0	0.0	0.0031322678402230177	0.0
5	0.0	0.0	0.0	0.00548146872039028	0.0
6	0.0	0.0	0.0	0.00548146872039028	0.0
7	0.0	0.0	0.0	0.00548146872039028	0.0
8	0.0	0.0	0.0	0.00704760264050179	0.0
9	0.0	0.0	0.0	0.007830669600557543	0.0
10	0.0	0.0	0.0	0.011746004400836315	0.0
11	0.0	0.0	0.0	0.01252907136089207	0.0
12	0.0	0.0	0.0	0.014878272241059334	0.0
13	0.0	0.0	0.0	0.015661339201115086	0.0
14	0.0	0.0	0.0	0.01801054008128235	0.0
15	0.0	0.0	0.0	0.021142807921505368	0.0
16	0.0	0.0	0.0	0.021142807921505368	0.0
17	0.0	0.0	0.0	0.02192587488156112	0.0
18	0.0	0.0	0.0	0.02349200880167263	0.0
19	0.0	0.0	0.0	0.024275075761728385	0.0
20	0.0	0.0	0.0	0.02505814272178414	0.0
21	0.0	0.0	0.0	0.02505814272178414	0.0
22	0.0	0.0	0.0	0.02819041056200716	0.0
23	0.0	0.0	0.0	0.03132267840223017	0.0
24	0.0	0.0	0.0	0.032888812322341685	0.0
25	0.0	0.0	0.0	0.03837028104273196	0.0
26	0.0	0.0	0.0	0.04933321848351253	0.0
27	0.0	0.0	0.0	0.11824311096841891	0.0
28	0.0	0.0	0.0	0.18323766865304653	0.0
29	0.0	0.0	0.0	0.24823222633767414	0.0
30	0.0	0.0	0.0	0.32027438666280356	0.0
31	0.0	0.0	0.0	0.39544881482815597	0.0
32	0.0	0.0	0.0	0.5003797874756271	0.0
33	0.0	0.0	0.0	0.6515117107663876	0.0
34	0.0	0.0	0.0	0.889564066623337	0.0
35	0.0	0.0	0.0	1.1980924488853042	0.0
36	0.0	0.0	0.0	1.6679326249187567	0.0
37	0.0	0.0	0.0	2.3202274026452003	0.0
38	0.0	0.0	0.0	2.993664988293149	0.0
39	0.0	0.0	0.0	3.70547285498383	0.0
40	0.0	0.0	0.0	4.512814890801312	0.0
41	0.0	0.0	0.0	5.2700406411752265	0.0
42	0.0	0.0	0.0	6.112620690195219	0.0
43	0.0	0.0	0.0	6.979475814976938	0.0
44	0.0	0.0	0.0	7.898013359122339	0.0
45	0.0	0.0	0.0	8.770349952624448	0.0
46	0.0	0.0	0.0	9.604316265083828	0.0
47	0.0	0.0	0.0	10.464906854185102	0.0
48	0.0	0.0	0.0	11.257370617761525	0.0
49	0.0	0.0	0.0	12.03104077429661	0.0
50	0.0	0.0	0.0	12.692732355543722	0.0
51	0.0	0.0	0.0	13.426466097115965	0.0
52	0.0	0.0	0.0	14.134358629006366	0.0
53	0.0	0.0	0.0	14.922907057782512	0.0
54	0.0	0.0	0.0	15.626101187912578	0.0
55	0.0	0.0	0.0	16.31754931364181	0.0
56	0.0	0.0	0.0	16.95183355128697	0.0
57	0.0	0.0	0.0	17.56419191405057	0.0
58	0.0	0.0	0.0	18.16558733937339	0.0
59	0.0	0.0	0.0	18.80691917965905	0.0
60	0.0	0.0	0.0	19.340970846417076	0.0
61	0.0	0.0	0.0	19.912609727257777	0.0
62	0.0	0.0	0.0	20.431783121774743	0.0
63	0.0	0.0	0.0	21.001855868695333	0.0
64	0.0	0.0	0.0	21.593071423537427	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATTTAC	15	0.0022203662	70.0	36
GCTTTTG	15	0.0022203662	70.0	44
ATTTACT	20	0.006937966	52.5	37
ACAGTCG	20	0.006937966	52.5	55
TGCATCC	20	0.006937966	52.5	33
CCGTATC	20	0.006937966	52.5	63
GGAACCG	20	0.006937966	52.5	5
GTATCGA	20	0.006937966	52.5	65
TAAGGGA	20	0.006937966	52.5	37
GACTGTT	20	0.006937966	52.5	49
CGACTAA	20	0.006937966	52.5	29
GATTATC	20	0.006937966	52.5	56
TCTTTAT	110	0.0	50.90909	1
TGGACAG	35	2.0769676E-5	50.0	5
CCGTCTT	2595	0.0	49.49904	47
TCTTCTG	2610	0.0	49.482758	50
CTGCTTG	2605	0.0	49.309025	54
CTCGTAT	2615	0.0	49.254303	39
CTTGAAA	2595	0.0	49.229286	57
CGTCTTC	2620	0.0	49.160305	48
>>END_MODULE