FastQCFastQC Report
Wed 25 May 2016
SRR1779723_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779723_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences145273
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGA308.346849E-658.32931523
GCCGTAT850.057.6430954
CGTCTGA407.442286E-752.49638456
AAGTGTC200.00694176852.49638434
GAGATAT200.00694176852.49638432
CGTCTCA451.6763588E-646.66345656
ACTGAGG305.864352E-446.6634521
ATACACG305.864352E-446.66345251
TTCCGAC703.8016879E-1044.99690219
ACGCTTC802.910383E-1143.74698615
AGTGTCG404.5767723E-543.74698635
CGTATCA1050.043.3303556
GTCTGAC503.461495E-641.99711257
TCTCGGT1850.041.61875543
CCGTATC1200.040.8305255
TGCCTAC1200.040.8305219
CGCCGTA1300.040.38183653
CTAGTGT350.00125257539.99724633
GACGCTT701.909575E-839.99724614
TAGTGTC350.00125257539.99724634
Produced by FastQC (version 0.11.5)