##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779723_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	145273
Sequences flagged as poor quality	0
Sequence length	76
%GC	56
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.81044653858597	33.0	23.0	34.0	12.0	34.0
2	27.37262257955711	33.0	23.0	34.0	12.0	34.0
3	26.40435593675356	32.0	21.0	34.0	11.0	34.0
4	28.57727864090368	33.0	23.0	34.0	12.0	34.0
5	28.074150048529322	34.0	23.0	34.0	11.0	34.0
6	30.288553275557053	36.0	26.0	38.0	11.0	38.0
7	31.341563814335768	36.0	27.0	38.0	12.0	38.0
8	30.60687808470948	36.0	26.0	38.0	11.0	38.0
9	32.10307490036001	37.0	31.0	38.0	12.0	38.0
10-11	31.676164875785588	37.0	27.5	38.0	11.0	38.0
12-13	30.818985633944365	35.5	27.0	38.0	11.0	38.0
14-15	30.733188548457044	35.5	26.5	38.0	11.0	38.0
16-17	30.40211532769338	35.5	26.5	38.0	11.0	38.0
18-19	30.914375004302244	36.0	26.5	38.0	11.5	38.0
20-21	29.78863932045184	35.0	24.0	38.0	11.0	38.0
22-23	29.80386582503287	35.5	24.5	37.5	11.0	38.0
24-25	30.94717531819402	36.0	26.0	38.0	11.0	38.0
26-27	29.52074714503039	34.5	23.0	38.0	11.0	38.0
28-29	31.640635217831253	37.0	29.0	38.0	11.0	38.0
30-31	32.08765565521467	37.0	31.0	38.0	11.0	38.0
32-33	32.21716698904821	37.0	31.0	38.0	11.0	38.0
34-35	32.29260082740771	37.0	31.0	38.0	11.0	38.0
36-37	30.08052081253915	35.0	24.0	38.0	11.0	38.0
38-39	28.372918573995168	34.0	21.0	37.0	11.0	38.0
40-41	29.54732813392716	34.0	24.0	37.5	11.0	38.0
42-43	30.606805806997862	35.5	25.0	38.0	11.0	38.0
44-45	32.30574504553496	37.0	31.0	38.0	11.0	38.0
46-47	30.86055908530835	35.5	26.5	38.0	11.0	38.0
48-49	30.153751901592173	35.0	24.0	38.0	11.0	38.0
50-51	27.936350870430154	33.0	21.0	37.0	11.0	38.0
52-53	31.29872378212056	36.0	27.5	38.0	11.0	38.0
54-55	30.325108588657216	35.5	24.5	38.0	11.0	38.0
56-57	30.79481390210156	36.0	27.0	38.0	11.0	38.0
58-59	31.912038024959905	37.0	29.5	38.0	11.0	38.0
60-61	32.45656109531709	37.0	31.0	38.0	11.0	38.0
62-63	32.30781356480557	37.0	31.0	38.0	11.0	38.0
64-65	31.99137485974682	37.0	29.0	38.0	11.0	38.0
66-67	29.905120015419243	35.5	23.5	38.0	11.0	38.0
68-69	29.99139206872578	35.0	24.0	38.0	11.0	38.0
70-71	29.54983376126328	34.5	23.0	38.0	11.0	38.0
72-73	28.36699868523401	33.5	21.5	37.0	11.0	38.0
74-75	30.386210789341447	35.5	25.0	37.5	11.0	38.0
76	30.66121715666366	36.0	25.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	2.0
4	1.0
5	1.0
6	0.0
7	1.0
8	0.0
9	1.0
10	0.0
11	3.0
12	50.0
13	227.0
14	657.0
15	1161.0
16	1979.0
17	3004.0
18	4102.0
19	4412.0
20	4054.0
21	3527.0
22	2952.0
23	2749.0
24	2732.0
25	3101.0
26	3317.0
27	3751.0
28	4412.0
29	5158.0
30	5959.0
31	7225.0
32	8971.0
33	11341.0
34	14394.0
35	17916.0
36	18574.0
37	9538.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.059749574938216	16.389831558513972	10.75836528467093	32.79205358187688
2	20.952276059556834	14.861674227144755	38.071768325841695	26.114281387456717
3	22.468197587972906	14.684867008095159	34.25987113827854	28.587064265653396
4	17.971804614791566	16.39545129137067	27.51321658681645	38.11952750702131
5	17.47193215532136	26.302891796823914	35.15312549475815	21.072050553096584
6	32.470589854962725	29.579481390210155	13.931012645157736	24.01891610966938
7	30.58723919792391	22.388193263717277	17.17111920315544	29.853448335203375
8	25.288432414571687	30.23308643335077	16.35873007131646	28.119751080761073
9	24.35705041716001	13.600379987333755	17.280474708814054	44.76209488669218
10-11	28.846881948412236	20.661609312507316	23.25855149483365	27.2329572442468
12-13	26.7073733194738	16.06834311991023	26.185231332649533	31.039052227966437
14-15	26.77568356417105	19.062009527218656	20.472850180356307	33.68945672825399
16-17	28.469004922039034	18.791518948129283	22.992806250645373	29.746669879186317
18-19	26.386857168426086	18.238329128389054	23.982638685716843	31.392175017468016
20-21	27.622489545149463	18.52169273925689	23.85358046362745	30.0022372519662
22-23	28.44633828170982	17.594558965762925	24.198628038811425	29.760474713715823
24-25	27.021938610439943	20.637919996695786	19.81875004302363	32.52139134984064
26-27	26.857416241593185	20.155366939952778	23.683304765641672	29.303912052812368
28-29	27.397637470055347	20.621885067599198	21.730181457719524	30.25029600462593
30-31	26.985853440264346	20.159019722575984	21.87209582487179	30.98303101228789
32-33	26.916828438863877	20.286577726377818	22.419905552572523	30.376688282185782
34-35	26.893423099692974	20.3836410447042	22.471190781049934	30.25174507455289
36-37	27.71742871697438	17.715088183057286	26.846268225186897	27.721214874781435
38-39	26.69078342758604	16.992334725037946	30.856259745087446	25.460622102288568
40-41	26.840853471746563	20.218496477140132	22.35389683028661	30.586753220826697
42-43	24.243550690966664	20.351420654998538	23.749290102741494	31.655738551293304
44-45	24.272369308716424	18.772802995883414	26.393994465325676	30.560833230074486
46-47	25.822106879057184	18.29803052310573	27.08392133106624	28.795941266770843
48-49	26.075957209532856	18.901876557487643	26.694477716740323	28.327688516239174
50-51	27.122721875161343	17.626791952776774	29.15036054175917	26.100125630302717
52-53	22.93868812622405	20.635319466769467	26.043348214132056	30.382644192874427
54-55	24.885382677295446	19.369983340905648	25.615422741728967	30.129211240069942
56-57	24.83057002130567	20.02767327635759	25.124856728839752	30.016899973496987
58-59	23.353021353930032	21.061363292167474	25.097751710654936	30.487863643247557
60-61	23.408517507598155	19.894606771738644	24.774636785747475	31.922238934915725
62-63	23.096251015378684	20.91921027632068	24.749425192405656	31.23511351589498
64-65	23.73414562789337	21.37023078114513	23.68251673636567	31.213106854595836
66-67	22.12592245842053	23.42459246613063	20.124394206410397	34.32509086903844
68-69	22.457663587759193	23.783441376247545	20.23644356923755	33.52245146675571
70-71	22.10270884246033	23.89632740164527	19.473376243417203	34.5275875124772
72-73	21.28425588743486	24.61329827145876	18.41505641336298	35.6873894277434
74-75	23.1415256045764	23.004880668011317	20.519801469019118	33.33379225839316
76	23.388656749296125	21.71587490620719	20.542862453275692	34.352605891220996
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.5
3	1.0
4	1.0
5	1.0
6	0.5
7	0.0
8	0.0
9	0.5
10	0.5
11	0.0
12	0.0
13	0.0
14	0.5
15	1.5
16	2.0
17	2.0
18	5.0
19	10.0
20	15.0
21	18.0
22	28.5
23	48.0
24	80.0
25	103.0
26	136.0
27	201.5
28	294.5
29	355.0
30	431.0
31	629.0
32	868.0
33	985.0
34	1190.5
35	1551.0
36	1973.0
37	2240.0
38	2538.0
39	3096.0
40	3736.0
41	4325.0
42	4534.0
43	4767.0
44	5172.5
45	5439.0
46	5533.0
47	5477.5
48	5365.0
49	5216.5
50	5125.0
51	4881.5
52	4405.0
53	4139.0
54	4106.0
55	4097.5
56	4006.0
57	4007.0
58	4091.0
59	4174.0
60	4407.0
61	4672.0
62	4787.0
63	4899.0
64	5079.0
65	5105.5
66	5019.5
67	4975.0
68	4880.0
69	4663.5
70	4408.5
71	4275.0
72	4009.5
73	3535.0
74	3144.5
75	2963.0
76	2656.5
77	2074.5
78	1538.0
79	1277.0
80	1080.5
81	731.0
82	464.5
83	351.0
84	273.0
85	154.0
86	85.0
87	57.0
88	43.0
89	20.5
90	9.5
91	5.0
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	1.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	6.883591582744213E-4
4	6.883591582744213E-4
5	0.0
6	0.0
7	0.0
8	0.003441795791372106
9	0.003441795791372106
10-11	0.004130154949646528
12-13	0.004130154949646528
14-15	0.003441795791372106
16-17	0.00550687326619537
18-19	0.005162693687058159
20-21	0.003785975370509317
22-23	0.004474334528783738
24-25	0.002753436633097685
26-27	0.002753436633097685
28-29	0.003441795791372106
30-31	0.00550687326619537
32-33	0.0048185141079209485
34-35	0.0048185141079209485
36-37	0.0048185141079209485
38-39	0.005162693687058159
40-41	0.005162693687058159
42-43	0.003785975370509317
44-45	0.0048185141079209485
46-47	0.004130154949646528
48-49	0.0048185141079209485
50-51	0.003785975370509317
52-53	0.005162693687058159
54-55	0.0048185141079209485
56-57	0.004474334528783738
58-59	0.0048185141079209485
60-61	0.005162693687058159
62-63	0.0048185141079209485
64-65	0.003785975370509317
66-67	0.006195232424469791
68-69	0.0258134684352908
70-71	0.00550687326619537
72-73	0.004130154949646528
74-75	0.004130154949646528
76	0.004130154949646528
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	145273.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	95.89966573650304
#Duplication Level	Percentage of deduplicated	Percentage of total
1	96.78770233864783	92.8190830168048
2	2.5273776347624315	4.847493407272617
3	0.465266078389897	1.338565841883742
4	0.13072495848155022	0.5014591928719561
5	0.053998768237465765	0.25892319120779317
6	0.01597310513961554	0.09190892661978894
7	0.009978255659963289	0.06698379676966944
8	0.003990713857236645	0.030616649996722
9	0.002992903793677482	0.025831662605566415
>10	0.0019952430303498956	0.019134313967364234
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	6.883591582744213E-4	0.0
2	0.0	0.0	0.0	6.883591582744213E-4	0.0
3	0.0	0.0	0.0	6.883591582744213E-4	0.0
4	0.0	0.0	0.0	0.002065077474823264	0.0
5	0.0	0.0	0.0	0.004130154949646528	0.0
6	0.0	0.0	0.0	0.004130154949646528	0.0
7	0.0	0.0	0.0	0.004130154949646528	0.0
8	0.0	0.0	0.0	0.006195232424469791	0.0
9	0.0	0.0	0.0	0.008948669057567476	0.0
10	0.0	0.0	0.0	0.009637028215841897	0.0
11	0.0	0.0	0.0	0.01032538737411632	0.0
12	0.0	0.0	0.0	0.01101374653239074	0.0
13	0.0	0.0	0.0	0.01101374653239074	0.0
14	0.0	0.0	0.0	0.011702105690665161	0.0
15	0.0	0.0	0.0	0.011702105690665161	0.0
16	0.0	0.0	0.0	0.011702105690665161	6.883591582744213E-4
17	0.0	0.0	0.0	0.011702105690665161	6.883591582744213E-4
18	0.0	0.0	0.0	0.012390464848939582	6.883591582744213E-4
19	0.0	0.0	0.0	0.015143901482037267	6.883591582744213E-4
20	0.0	0.0	0.0	0.01583226064031169	6.883591582744213E-4
21	0.0	0.0	0.0	0.017897338115134952	6.883591582744213E-4
22	0.0	0.0	0.0	0.03304123959717222	6.883591582744213E-4
23	0.0	0.0	0.0	0.035794676230269905	6.883591582744213E-4
24	0.0	0.0	0.0	0.04061319033819086	6.883591582744213E-4
25	0.0	0.0	0.0	0.0419899086547397	6.883591582744213E-4
26	0.0	0.0	0.0	0.04474334528783738	6.883591582744213E-4
27	0.0	0.0	0.0	0.08122638067638172	6.883591582744213E-4
28	0.0	0.0	0.0	0.13698347249660983	6.883591582744213E-4
29	0.0	0.0	0.0	0.18172681778444721	6.883591582744213E-4
30	0.0	0.0	0.0	0.2367955504464009	6.883591582744213E-4
31	0.0	0.0	0.0	0.26708335341047545	6.883591582744213E-4
32	0.0	0.0	0.0	0.32834731849689897	6.883591582744213E-4
33	0.0	0.0	0.0	0.3854811286336759	6.883591582744213E-4
34	0.0	0.0	0.0	0.47152602341797856	6.883591582744213E-4
35	0.0	0.0	0.0	0.6656433060513653	6.883591582744213E-4
36	0.0	0.0	0.0	0.8108870884472682	6.883591582744213E-4
37	0.0	0.0	0.0	1.2294094566781164	6.883591582744213E-4
38	0.0	0.0	0.0	1.6293461276355552	6.883591582744213E-4
39	0.0	0.0	0.0	2.080221376305301	6.883591582744213E-4
40	0.0	0.0	0.0	2.58134684352908	6.883591582744213E-4
41	0.0	0.0	0.0	3.0845373882276816	0.0013767183165488426
42	0.0	0.0	0.0	3.592546447034205	0.0013767183165488426
43	0.0	0.0	0.0	4.136350182070998	0.0013767183165488426
44	0.0	0.0	0.0	4.689102586165357	0.0013767183165488426
45	0.0	0.0	0.0	5.052556221734252	0.0013767183165488426
46	0.0	0.0	0.0	5.508938343670193	0.0013767183165488426
47	0.0	0.0	0.0	6.023830994059461	0.0013767183165488426
48	0.0	0.0	0.0	6.374894164779415	0.0013767183165488426
49	0.0	0.0	0.0	6.73215256792384	0.0013767183165488426
50	0.0	0.0	0.0	7.2257060844066	0.0013767183165488426
51	0.0	0.0	0.0	7.740598734795867	0.0013767183165488426
52	0.0	0.0	0.0	8.181148596091496	0.0013767183165488426
53	0.0	0.0	0.0	8.711873507121075	0.0013767183165488426
54	0.0	0.0	0.0	9.232961389934813	0.0013767183165488426
55	0.0	0.0	0.0	9.57645260991375	0.0013767183165488426
56	0.0	0.0	0.0	9.95023163285676	0.0013767183165488426
57	0.0	0.0	0.0	10.271695359770915	0.0013767183165488426
58	0.0	0.0	0.0	10.617251657224674	0.0013767183165488426
59	0.0	0.0	0.0	10.98414708858494	0.0013767183165488426
60	0.0	0.0	0.0	11.201668582599657	0.0013767183165488426
61	0.0	0.0	0.0	11.421943513247472	0.0013767183165488426
62	0.0	0.0	0.0	11.586461352075059	0.0013767183165488426
63	0.0	0.0	0.0	11.79228074039911	0.0013767183165488426
64	0.0	0.0	0.0	11.99603505124834	0.0013767183165488426
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGAGA	30	8.346849E-6	58.329315	23
GCCGTAT	85	0.0	57.64309	54
CGTCTGA	40	7.442286E-7	52.496384	56
AAGTGTC	20	0.006941768	52.496384	34
GAGATAT	20	0.006941768	52.496384	32
CGTCTCA	45	1.6763588E-6	46.663456	56
ACTGAGG	30	5.864352E-4	46.663452	1
ATACACG	30	5.864352E-4	46.663452	51
TTCCGAC	70	3.8016879E-10	44.996902	19
ACGCTTC	80	2.910383E-11	43.746986	15
AGTGTCG	40	4.5767723E-5	43.746986	35
CGTATCA	105	0.0	43.33035	56
GTCTGAC	50	3.461495E-6	41.997112	57
TCTCGGT	185	0.0	41.618755	43
CCGTATC	120	0.0	40.83052	55
TGCCTAC	120	0.0	40.83052	19
CGCCGTA	130	0.0	40.381836	53
CTAGTGT	35	0.001252575	39.997246	33
GACGCTT	70	1.909575E-8	39.997246	14
TAGTGTC	35	0.001252575	39.997246	34
>>END_MODULE