FastQCFastQC Report
Wed 25 May 2016
SRR1779723_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779723_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences145273
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT1607611.066061828419596No Hit
TCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG18521.2748411611242283No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC11960.8232775532962079No Hit
ATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTCTT7080.48735828405829024RNA PCR Primer, Index 30 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGACCTCGTATGCCGT4220.29048756479180576No Hit
TCTTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC4130.284292332367336No Hit
CTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTC4020.27672038162631735No Hit
CTTTACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTC2670.18379189525927048No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGAACTCGTATGCCGT1730.11908613438147486No Hit
TCTTAATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC1650.11357926111527951No Hit
TTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTC1590.10944910616563297No Hit
CTTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG1560.10738402869080972No Hit
CTTAATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG1530.10531895121598646No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCAAT150.002220982570.06
TATGGGA207.905357E-570.02
TAGCGCT150.002220982570.058
GCAGTAA150.002220982570.052
TAACACG150.002220982570.025
CATACCA150.002220982570.040
TGTAACA150.002220982570.023
GTAACAC150.002220982570.024
GGTACCT200.00693987752.560
TTTGTAA200.00693987752.523
TTGTATA200.00693987752.52
TTTCCTT200.00693987752.522
AAGCGGT200.00693987752.511
ATGTAAC200.00693987752.522
TACTGTG200.00693987752.531
CGCTAAT200.00693987752.548
TCCAATT200.00693987752.570
AATTACT200.00693987752.528
GCGGATA200.00693987752.511
ATAACGC200.00693987752.544
Produced by FastQC (version 0.11.5)