##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779723_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	145273
Sequences flagged as poor quality	0
Sequence length	76
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.65113957858652	34.0	34.0	34.0	34.0	34.0
2	33.65160077922257	34.0	34.0	34.0	34.0	34.0
3	33.652523180494654	34.0	34.0	34.0	34.0	34.0
4	33.68879282454414	34.0	34.0	34.0	34.0	34.0
5	33.67802000371714	34.0	34.0	34.0	34.0	34.0
6	37.30585862479607	38.0	38.0	38.0	36.0	38.0
7	37.31756073048674	38.0	38.0	38.0	36.0	38.0
8	37.20926806770701	38.0	38.0	38.0	36.0	38.0
9	37.22064664459328	38.0	38.0	38.0	36.0	38.0
10-11	37.11979858611029	38.0	38.0	38.0	36.0	38.0
12-13	37.08707743352171	38.0	38.0	38.0	35.5	38.0
14-15	37.127563277415625	38.0	38.0	38.0	36.0	38.0
16-17	37.09092191942068	38.0	38.0	38.0	35.5	38.0
18-19	37.09991533182354	38.0	38.0	38.0	36.0	38.0
20-21	37.12021160160525	38.0	38.0	38.0	36.0	38.0
22-23	37.0551719865357	38.0	38.0	38.0	35.0	38.0
24-25	37.055361285304215	38.0	38.0	38.0	35.0	38.0
26-27	36.929233236733594	38.0	38.0	38.0	35.0	38.0
28-29	36.87520736819643	38.0	37.5	38.0	34.5	38.0
30-31	36.86902934475092	38.0	37.0	38.0	35.0	38.0
32-33	36.72214726755832	38.0	37.0	38.0	34.0	38.0
34-35	36.70158597950066	38.0	37.0	38.0	34.0	38.0
36-37	36.69123649955601	38.0	37.0	38.0	34.0	38.0
38-39	35.89759280802352	38.0	37.0	38.0	31.0	38.0
40-41	36.557398828412715	38.0	37.0	38.0	34.0	38.0
42-43	36.81443557990818	38.0	37.0	38.0	34.5	38.0
44-45	36.80130857075988	38.0	37.0	38.0	34.5	38.0
46-47	36.851448651848585	38.0	37.0	38.0	35.0	38.0
48-49	36.832752817109856	38.0	37.0	38.0	34.5	38.0
50-51	36.75914657231557	38.0	37.0	38.0	34.0	38.0
52-53	36.73253804905247	38.0	37.0	38.0	34.0	38.0
54-55	36.72039883529631	38.0	37.0	38.0	34.0	38.0
56-57	36.75049733949186	38.0	37.0	38.0	34.0	38.0
58-59	36.653400838421454	38.0	37.0	38.0	34.0	38.0
60-61	36.72858686748398	38.0	37.0	38.0	34.0	38.0
62-63	36.74965065772717	38.0	37.0	38.0	34.5	38.0
64-65	36.78633331727162	38.0	37.0	38.0	35.0	38.0
66-67	36.728707330336675	38.0	37.0	38.0	34.0	38.0
68-69	36.5529761208208	38.0	37.0	38.0	34.0	38.0
70-71	35.55601866830037	38.0	36.5	38.0	29.0	38.0
72-73	33.85475277580831	38.0	36.0	38.0	20.5	38.0
74-75	33.66293805455935	38.0	36.0	38.0	19.0	38.0
76	33.16124813282578	38.0	34.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	1.0
21	8.0
22	12.0
23	24.0
24	41.0
25	76.0
26	153.0
27	328.0
28	546.0
29	946.0
30	1479.0
31	2244.0
32	3439.0
33	5615.0
34	9451.0
35	18185.0
36	21902.0
37	80822.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.05111755109346	14.158171167388298	10.495412086210102	37.29529919530815
2	20.374742725764595	14.916054600648435	38.842730583109045	25.866472090477927
3	20.400900373779024	16.0903953246646	34.24104961004454	29.26765469151184
4	15.808168069772085	23.862658580741087	27.391875985214043	32.93729736427278
5	16.99076910368754	27.63073661313527	37.602307379898534	17.776186903278653
6	32.032793430300195	37.4715191398264	13.620562664775973	16.87512476509744
7	28.8463788866479	22.3014600097747	16.027754641261623	32.82440646231578
8	24.727926042692037	38.42283149656164	14.60422790195012	22.245014558796196
9	22.401960446882764	13.436770769516704	14.597344310367378	49.56392447323316
10-11	27.38395985489389	24.089817102971647	23.684029379168877	24.842193662965588
12-13	25.629332360452388	15.278819877059055	25.252455721297146	33.83939204119141
14-15	24.368946741651925	15.652598900002065	16.888891948262927	43.08956241008309
16-17	32.157730617527	23.82789644324823	16.439393417909727	27.57497952131504
18-19	32.60206645419314	18.560572164132356	16.57018165798187	32.267179723692635
20-21	25.27310649604538	17.55006091978551	16.69683974310436	40.47999284106476
22-23	25.589063349693337	24.56031058765221	16.511326949949407	33.33929911270504
24-25	32.817867050312174	24.10668190234937	16.152003469330158	26.923447578008304
26-27	31.828694939871827	25.376704549365677	17.26955456278868	25.525045947973812
28-29	25.726391001769084	24.830835736854063	18.111761992937435	31.33101126843942
30-31	25.165034108196295	18.03569830594811	30.2699056259594	26.529361959896196
32-33	31.865866334418648	17.00901062138181	19.632003193986495	31.49311985021305
34-35	32.15050284636512	17.181788770108692	17.734196994623915	32.933511388902275
36-37	33.17443709429832	22.840445230703573	17.70528590997639	26.279831765021715
38-39	24.657713408548044	18.512731202632285	25.35433287672176	31.47522251209791
40-41	24.02029282798593	16.6675844788777	26.72003744673821	32.59208524639816
42-43	30.822313850474625	16.22565789926552	26.220632877410115	26.731395372849736
44-45	23.270669704625085	23.571482656791005	27.798007888595954	25.359839749987955
46-47	29.65451253846207	17.447839584781754	21.727024292194695	31.17062358456148
48-49	29.13273629649006	16.790800768208822	20.206094731987363	33.87036820331376
50-51	23.067259573354992	17.16595650946838	27.399447935955063	32.367335981221565
52-53	21.409690720230188	17.49051785259477	34.3054111913432	26.79438023583185
54-55	21.130216901970773	17.250280506356997	28.680140149924625	32.939362441747605
56-57	27.846881388833438	17.37315261610898	21.947987582000785	32.831978413056795
58-59	22.22126616783573	18.21329496878291	32.419651277250416	27.145787586130936
60-61	28.214465179351976	24.775078645033833	20.869328780984766	26.14112739462942
62-63	21.16360232114708	33.653535068457316	18.29589806777584	26.886964542619758
64-65	20.825962154013478	34.743551795584864	17.846055357843507	26.584430692558147
66-67	21.28578607174079	34.50813296345501	17.1828213088461	27.023259655958093
68-69	20.94057395386617	35.40196733047435	17.285731003008127	26.371727712651357
70-71	21.51328877355049	33.8707123828929	17.594460085494205	27.02153875806241
72-73	21.822017856036567	29.831765021717732	19.158067913514557	29.188149208731147
74-75	22.137974709684524	27.733302127718158	20.32380414805229	29.80491901454503
76	22.516916426314594	27.426982302286042	20.0140425268288	30.042058744570564
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	2.5
16	2.5
17	0.0
18	6.5
19	11.0
20	15.0
21	21.0
22	24.0
23	49.5
24	101.5
25	131.0
26	167.0
27	223.5
28	264.5
29	285.0
30	373.5
31	522.5
32	780.0
33	977.0
34	1098.0
35	1355.5
36	1684.5
37	1877.0
38	2083.0
39	2551.5
40	3132.0
41	6562.5
42	9675.0
43	10477.0
44	10394.5
45	8232.5
46	6955.0
47	6175.5
48	4827.0
49	3878.0
50	3498.0
51	3278.5
52	2736.5
53	2418.5
54	2423.0
55	2509.5
56	2568.5
57	2611.5
58	2682.0
59	2815.5
60	3176.0
61	3591.0
62	3779.0
63	3876.5
64	4048.5
65	4212.5
66	4331.0
67	4360.0
68	4307.5
69	4286.0
70	4221.5
71	4126.0
72	4183.0
73	4094.0
74	3667.5
75	3387.0
76	3258.5
77	2854.0
78	2242.5
79	1907.0
80	1683.5
81	1225.5
82	859.0
83	727.0
84	585.0
85	321.0
86	165.5
87	132.0
88	91.5
89	39.0
90	16.0
91	2.5
92	0.0
93	0.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	145273.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.79666558823732
#Duplication Level	Percentage of deduplicated	Percentage of total
1	53.28765360612494	22.805338913631577
2	25.096506465933217	21.480935893111592
3	11.43440777198739	14.680635768518583
4	4.801196680177572	8.21900834979659
5	2.1520941903107507	4.605122768855878
6	1.0036672457054623	2.5772166885794334
7	0.543653091423792	1.6286577684772807
8	0.41658624461172233	1.4262801759446009
9	0.24448304703081772	0.9416753285194084
>10	0.9891912758154797	5.971515698030604
>50	0.004825323296660876	0.1390485499714331
>100	0.019301293186643503	1.8730252696647003
>500	0.001608441098886959	0.48735828405829024
>1k	0.003216882197773918	2.098118714420436
>5k	0.0	0.0
>10k+	0.001608441098886959	11.066061828419596
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT	16076	11.066061828419596	No Hit
TCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG	1852	1.2748411611242283	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC	1196	0.8232775532962079	No Hit
ATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTCTT	708	0.48735828405829024	RNA PCR Primer, Index 30 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGACCTCGTATGCCGT	422	0.29048756479180576	No Hit
TCTTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC	413	0.284292332367336	No Hit
CTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTC	402	0.27672038162631735	No Hit
CTTTACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTC	267	0.18379189525927048	No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGAACTCGTATGCCGT	173	0.11908613438147486	No Hit
TCTTAATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC	165	0.11357926111527951	No Hit
TTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTC	159	0.10944910616563297	No Hit
CTTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG	156	0.10738402869080972	No Hit
CTTAATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG	153	0.10531895121598646	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.002065077474823264	0.0
2	0.0	0.0	0.0	0.002753436633097685	0.0
3	0.0	0.0	0.0	0.002753436633097685	0.0
4	0.0	0.0	0.0	0.0048185141079209485	0.0
5	0.0	0.0	0.0	0.008260309899293055	0.0
6	0.0	0.0	0.0	0.008260309899293055	0.0
7	0.0	0.0	0.0	0.008260309899293055	0.0
8	0.0	0.0	0.0	0.01032538737411632	0.0
9	0.0	0.0	0.0	0.013767183165488426	0.0
10	0.0	0.0	0.0	0.01652061979858611	0.0
11	0.0	0.0	0.0	0.018585697273409373	0.0
12	0.0	0.0	0.0	0.019274056431683794	0.0
13	0.0	0.0	0.0	0.019274056431683794	0.0
14	0.0	0.0	0.0	0.019962415589958215	0.0
15	0.0	0.0	0.0	0.02065077474823264	0.0
16	0.0	0.0	0.0	0.02202749306478148	0.0
17	0.0	0.0	0.0	0.022715852223055902	0.0
18	0.0	0.0	0.0	0.024092570539604743	0.0
19	0.0	0.0	0.0	0.028911084647525693	6.883591582744213E-4
20	0.0	0.0	0.0	0.030287802964074535	6.883591582744213E-4
21	0.0	0.0	0.0	0.03304123959717222	6.883591582744213E-4
22	0.0	0.0	0.0	0.035106317071995484	6.883591582744213E-4
23	0.0	0.0	0.0	0.04130154949646528	6.883591582744213E-4
24	0.0	0.0	0.0	0.04405498612956296	6.883591582744213E-4
25	0.0	0.0	0.0	0.046120063604386224	6.883591582744213E-4
26	0.0	0.0	0.0	0.05093857771230717	6.883591582744213E-4
27	0.0	0.0	0.0	0.10256551458288876	6.883591582744213E-4
28	0.0	0.0	0.0	0.1810384586261728	0.0034417957913721064
29	0.0	0.0	0.0	0.27396694499321966	0.0034417957913721064
30	0.0	0.0	0.0	0.35381660735305254	0.0034417957913721064
31	0.0	0.0	0.0	0.4433032979287273	0.0034417957913721064
32	0.0	0.0	0.0	0.550687326619537	0.0034417957913721064
33	0.0	0.0	0.0	0.694554390698891	0.0034417957913721064
34	0.0	0.0	0.0	0.9031272156560407	0.0034417957913721064
35	0.0	0.0	0.0	1.2438649990018793	0.0034417957913721064
36	0.0	0.0	0.0	1.7312232830601695	0.0034417957913721064
37	0.0	0.0	0.0	2.4471168076655676	0.0034417957913721064
38	0.0	0.0	0.0	3.218767424091194	0.0034417957913721064
39	0.0	0.0	0.0	3.938102744487964	0.0034417957913721064
40	0.0	0.0	0.0	4.740729523035939	0.0034417957913721064
41	0.0	0.0	0.0	5.4993013154543515	0.0034417957913721064
42	0.0	0.0	0.0	6.269575213563429	0.0034417957913721064
43	0.0	0.0	0.0	7.052239576521446	0.0034417957913721064
44	0.0	0.0	0.0	7.841099171903933	0.0034417957913721064
45	0.0	0.0	0.0	8.626516971495047	0.0034417957913721064
46	0.0	0.0	0.0	9.441534214891963	0.0034417957913721064
47	0.0	0.0	0.0	10.21662662710896	0.0034417957913721064
48	0.0	0.0	0.0	10.925636560131615	0.0034417957913721064
49	0.0	0.0	0.0	11.669752810226264	0.0034417957913721064
50	0.0	0.0	0.0	12.349163299443118	0.0034417957913721064
51	0.0	0.0	0.0	13.002416140645543	0.0034417957913721064
52	0.0	0.0	0.0	13.64190179868248	0.0034417957913721064
53	0.0	0.0	0.0	14.260736681971185	0.0034417957913721064
54	0.0	0.0	0.0	14.885078438526085	0.0034417957913721064
55	0.0	0.0	0.0	15.488769420332753	0.0034417957913721064
56	0.0	0.0	0.0	16.07043290907464	0.0034417957913721064
57	0.0	0.0	0.0	16.641082651284133	0.0034417957913721064
58	0.0	0.0	0.0	17.25372230214837	0.0034417957913721064
59	0.0	0.0	0.0	17.804409628767907	0.0034417957913721064
60	0.0	0.0	0.0	18.314483765049253	0.0034417957913721064
61	0.0	0.0	0.0	18.8218044646975	0.0034417957913721064
62	0.0	0.0	0.0	19.31053946707234	0.0034417957913721064
63	0.0	0.0	0.0	19.812353293454393	0.0034417957913721064
64	0.0	0.0	0.0	20.31485547899472	0.0034417957913721064
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCAAT	15	0.0022209825	70.0	6
TATGGGA	20	7.905357E-5	70.0	2
TAGCGCT	15	0.0022209825	70.0	58
GCAGTAA	15	0.0022209825	70.0	52
TAACACG	15	0.0022209825	70.0	25
CATACCA	15	0.0022209825	70.0	40
TGTAACA	15	0.0022209825	70.0	23
GTAACAC	15	0.0022209825	70.0	24
GGTACCT	20	0.006939877	52.5	60
TTTGTAA	20	0.006939877	52.5	23
TTGTATA	20	0.006939877	52.5	2
TTTCCTT	20	0.006939877	52.5	22
AAGCGGT	20	0.006939877	52.5	11
ATGTAAC	20	0.006939877	52.5	22
TACTGTG	20	0.006939877	52.5	31
CGCTAAT	20	0.006939877	52.5	48
TCCAATT	20	0.006939877	52.5	70
AATTACT	20	0.006939877	52.5	28
GCGGATA	20	0.006939877	52.5	11
ATAACGC	20	0.006939877	52.5	44
>>END_MODULE