FastQCFastQC Report
Wed 25 May 2016
SRR1779722_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779722_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences185555
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTATA207.912189E-569.99811648
ATCTCGT401.0608346E-861.24835242
GACGCTT509.749783E-1055.9984914
TCGCATA200.006943637552.49858540
CTTAGAT200.006943637552.4985853
CGACGAT552.2700988E-950.9077222
CGTGTAT352.0809812E-549.99865335
TAGATCT1400.049.99865339
TCGTTGG506.165101E-848.9986845
TCGGTGG1000.048.9986845
ATCTCGG1100.047.72598642
GTAGATC1550.047.4180838
AGATCTC1350.046.6654140
GATCTCG1600.045.93626441
TGGTCGC1550.045.16007649
TCGTCAT404.580579E-543.748828
TTCGGTG404.580579E-543.7488244
ACGCTTC1050.043.33216515
GCAGTGT659.949872E-943.0757633
GCCGACT659.949872E-943.0757620