##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779722_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	185555
Sequences flagged as poor quality	0
Sequence length	76
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.392185605346125	34.0	27.0	34.0	12.0	34.0
2	27.91438118078198	34.0	23.0	34.0	12.0	34.0
3	26.818716822505458	32.0	21.0	34.0	11.0	34.0
4	29.128403977257417	34.0	23.0	34.0	12.0	34.0
5	28.764651989976016	34.0	23.0	34.0	11.0	34.0
6	31.115771604106598	37.0	27.0	38.0	11.0	38.0
7	31.998415564118456	37.0	28.0	38.0	12.0	38.0
8	31.24446121096171	37.0	27.0	38.0	11.0	38.0
9	32.66586187383795	37.0	31.0	38.0	12.0	38.0
10-11	32.390016437174964	37.0	31.0	38.0	11.0	38.0
12-13	31.419129099188922	36.0	28.5	38.0	11.0	38.0
14-15	31.412365605885046	36.5	27.5	38.0	11.0	38.0
16-17	31.068745116003342	36.0	27.0	38.0	11.0	38.0
18-19	31.690679313411117	37.0	27.5	38.0	11.5	38.0
20-21	30.532154886691277	35.5	25.0	38.0	11.0	38.0
22-23	30.51899436824661	35.5	26.0	38.0	11.0	38.0
24-25	31.691735603998815	36.5	29.0	38.0	11.0	38.0
26-27	30.2989086793673	35.5	24.5	38.0	11.0	38.0
28-29	32.35828191102369	37.0	31.0	38.0	11.0	38.0
30-31	32.801058985206545	37.5	33.0	38.0	11.0	38.0
32-33	32.90586079599041	38.0	34.0	38.0	11.0	38.0
34-35	32.96528522540487	38.0	34.0	38.0	11.0	38.0
36-37	30.91026919242273	36.5	27.0	38.0	11.0	38.0
38-39	29.18730565061572	34.0	23.0	37.5	11.0	38.0
40-41	30.371329255476812	35.0	25.0	38.0	11.0	38.0
42-43	31.369507693136804	36.5	28.0	38.0	11.0	38.0
44-45	32.979793053272616	37.5	34.0	38.0	11.0	38.0
46-47	31.602516774002318	37.0	29.0	38.0	11.0	38.0
48-49	31.05224327018943	36.5	27.0	38.0	11.0	38.0
50-51	28.872140335749506	34.0	21.5	37.5	11.0	38.0
52-53	32.166769960389104	37.0	29.5	38.0	11.0	38.0
54-55	31.279369998113765	36.5	27.5	38.0	11.0	38.0
56-57	31.673145428579126	37.0	29.0	38.0	11.0	38.0
58-59	32.73723693783515	37.0	33.0	38.0	11.0	38.0
60-61	33.18863409770688	38.0	34.0	38.0	11.0	38.0
62-63	33.09190805960497	38.0	34.0	38.0	11.0	38.0
64-65	32.809404219773114	37.5	32.5	38.0	11.0	38.0
66-67	30.724642289348175	36.5	25.0	38.0	11.0	38.0
68-69	30.83002344318396	36.5	26.0	38.0	11.0	38.0
70-71	30.4701085931395	36.0	25.0	38.0	11.0	38.0
72-73	29.266972056802565	34.0	22.5	37.5	11.0	38.0
74-75	31.37538465684029	36.5	28.0	38.0	11.0	38.0
76	31.798577241249227	37.0	28.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	1.0
4	0.0
5	1.0
6	1.0
7	1.0
8	0.0
9	0.0
10	2.0
11	11.0
12	56.0
13	310.0
14	842.0
15	1486.0
16	2411.0
17	3725.0
18	4815.0
19	5072.0
20	4623.0
21	3772.0
22	3094.0
23	2614.0
24	2540.0
25	2830.0
26	3192.0
27	3664.0
28	4376.0
29	5201.0
30	6401.0
31	8220.0
32	10563.0
33	14311.0
34	19168.0
35	25672.0
36	29759.0
37	16820.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.49634879146345	18.937781250842068	11.204764086120019	29.361105871574466
2	20.96736816577295	17.54466330737517	38.03023362345396	23.457734903397913
3	20.190348901128512	17.76679564978389	36.188387208036474	25.85446824105112
4	14.113950655873762	18.840876510342003	33.36656714487427	33.67860568890997
5	13.817466519360835	30.050928296192502	39.37538735145913	16.756217832987524
6	27.064751690873322	33.839023470130144	17.569992724529115	21.52623211446741
7	27.612298240413896	26.094149982484975	20.231198297000887	26.062353480100242
8	23.990169818540455	31.60963832046176	18.18529676477087	26.214895096226915
9	23.548115892041043	16.343666465465205	19.887686470638958	40.22053117185479
10-11	24.531728730069336	23.62711837003064	27.264422701219893	24.576730198680135
12-13	24.423126153680325	20.007814710518872	28.833856559641063	26.735202576159743
14-15	23.436889488902	22.676453706922338	23.690457905217688	30.19619889895797
16-17	24.808336454743152	23.081006647929225	26.371017507740646	25.739639389586976
18-19	22.172100555919343	23.07321266407435	28.023131418470104	26.731555361536202
20-21	24.211198568583754	22.882719259716357	27.267509384237716	25.63857278746217
22-23	25.129009396465072	21.871640029426267	27.454007981740624	25.54534259236803
24-25	22.1354833320309	24.903732407080554	24.846605102115056	28.11417915877349
26-27	23.097029404156245	24.052017763214625	27.432741226179186	25.41821160644994
28-29	23.081048016577697	24.68707979768311	26.36371230473645	25.868159881002743
30-31	22.573943130618492	24.802476987086898	26.39694310906073	26.22663677323388
32-33	22.78556396002145	24.638438799350038	26.401310701457025	26.17468653917149
34-35	22.932301448931693	24.381233141381994	26.450211667443636	26.236253742242678
36-37	24.27087403495062	22.018890041633544	29.908783465150435	23.801452458265405
38-39	23.720341152535067	21.26544415850389	33.02254139775529	21.991673291205757
40-41	23.32050639460622	23.146965458886427	26.14324133507952	27.389286811427834
42-43	20.933754419246355	23.937764076916444	27.31660127619212	27.81188022764508
44-45	20.85600260848242	22.87892383931964	29.039038310738164	27.22603524145978
46-47	22.967040962335993	21.60648024640458	29.867393161356738	25.559085629902693
48-49	22.943720610625313	22.286745981487222	30.196580390466053	24.572953017421415
50-51	24.733631184957236	20.94599328486508	31.396219907195327	22.92415562298236
52-53	19.592504365447216	23.873876301550006	29.606624700886027	26.926994632116756
54-55	21.866578998426284	22.86820660961045	28.877702804665102	26.387511587298167
56-57	21.67097817299919	23.372675828617623	28.213689032605764	26.742656965777417
58-59	19.855994524342695	24.222507861825886	28.74073355483876	27.18076405899266
60-61	19.624893895094246	23.25954270469826	28.964281383473235	28.151282016734257
62-63	19.556395110699118	23.942861146441892	28.544096406320733	27.956647336538254
64-65	20.18172852280903	24.525805504132276	27.466498519279014	27.825967453779676
66-67	19.033991387626855	25.353953445757675	23.9807164761489	31.631338690466563
68-69	19.25608474165117	25.16131640656586	24.30689199752028	31.275706854262687
70-71	19.42262451730429	25.044665353428925	23.400603083324125	32.13210704594266
72-73	18.872103864747316	25.433982398180532	22.032185568232652	33.6617281688395
74-75	20.425546821737594	25.42933287343809	23.909792265675737	30.235328039148584
76	20.32465992627245	24.346799749929936	24.20451850734042	31.124021816457198
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.5
3	0.5
4	0.0
5	1.0
6	2.0
7	1.0
8	0.0
9	0.0
10	0.5
11	1.0
12	1.0
13	0.5
14	0.0
15	2.0
16	3.0
17	2.0
18	4.5
19	8.5
20	16.5
21	23.0
22	37.5
23	72.5
24	127.5
25	162.0
26	211.0
27	343.5
28	569.0
29	711.0
30	851.0
31	1208.5
32	1727.5
33	2029.0
34	2434.5
35	3280.0
36	4194.5
37	4669.0
38	5349.0
39	6655.5
40	8095.0
41	9449.5
42	9991.0
43	10372.5
44	10979.5
45	11456.5
46	11708.0
47	11612.5
48	11237.5
49	10520.0
50	10082.0
51	9546.5
52	8381.5
53	7192.5
54	6633.0
55	6102.5
56	5116.0
57	4287.5
58	3915.0
59	3687.5
60	3239.5
61	2979.0
62	2939.0
63	2804.5
64	2598.0
65	2501.5
66	2339.0
67	2201.0
68	2138.5
69	2002.5
70	1840.0
71	1751.0
72	1616.5
73	1384.0
74	1198.5
75	1111.0
76	1028.5
77	823.0
78	611.5
79	523.0
80	460.0
81	320.0
82	212.5
83	182.0
84	136.5
85	75.5
86	46.0
87	32.0
88	26.0
89	14.5
90	7.5
91	4.5
92	3.0
93	1.5
94	0.5
95	0.5
96	0.0
97	0.0
98	1.0
99	2.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	5.38923769232842E-4
4	5.38923769232842E-4
5	0.0
6	0.0
7	0.0
8	0.002155695076931368
9	0.0016167713076985259
10-11	0.002964080730780631
12-13	0.004041928269246315
14-15	0.0018862331923149471
16-17	0.004580852038479157
18-19	0.003503004500013473
20-21	0.0024251569615477892
22-23	0.003503004500013473
24-25	0.0024251569615477892
26-27	0.0016167713076985259
28-29	0.0024251569615477892
30-31	0.0037724663846298943
32-33	0.002964080730780631
34-35	0.0024251569615477892
36-37	0.004041928269246315
38-39	0.004041928269246315
40-41	0.004311390153862736
42-43	0.0016167713076985259
44-45	0.003503004500013473
46-47	0.003503004500013473
48-49	0.004041928269246315
50-51	0.002155695076931368
52-53	0.0037724663846298943
54-55	0.0037724663846298943
56-57	0.00269461884616421
58-59	0.003503004500013473
60-61	0.004041928269246315
62-63	0.0037724663846298943
64-65	0.0018862331923149471
66-67	0.004311390153862736
68-69	0.0269461884616421
70-71	0.004580852038479157
72-73	0.0032335426153970517
74-75	0.002964080730780631
76	0.0037724663846298943
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	185555.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	88.00888214394142
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.5096737483658	81.41672974094398
2	4.853983143204522	8.543872607579303
3	1.3279096166578974	3.5060352285075402
4	0.5604443371242307	1.972963184568232
5	0.2724163227172196	1.1987528020052847
6	0.18131687370635854	0.9574497221238493
7	0.09526876488077494	0.5869148250273692
8	0.06340534321280385	0.44641867024894477
9	0.03959921933898	0.313657472501674
>10	0.09598263079142003	1.0572057464938374
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.001616771307698526	0.0
5	0.0	0.0	0.0	0.00269461884616421	0.0
6	0.0	0.0	0.0	0.00269461884616421	0.0
7	0.0	0.0	0.0	0.00269461884616421	0.0
8	0.0	0.0	0.0	0.00269461884616421	0.0
9	0.0	0.0	0.0	0.0037724663846298943	0.0
10	0.0	0.0	0.0	0.006467085230794104	0.0
11	0.0	0.0	0.0	0.0075449327692597885	0.0
12	0.0	0.0	0.0	0.0075449327692597885	0.0
13	0.0	0.0	0.0	0.008622780307725472	0.0
14	0.0	0.0	0.0	0.010239551615423998	0.0
15	0.0	0.0	0.0	0.01077847538465684	0.0
16	0.0	0.0	0.0	0.01077847538465684	0.0
17	0.0	0.0	0.0	0.01077847538465684	0.0
18	0.0	0.0	5.38923769232842E-4	0.011856322923122524	0.0
19	0.0	0.0	5.38923769232842E-4	0.011856322923122524	0.0
20	0.0	0.0	5.38923769232842E-4	0.014012018000053892	0.0
21	0.0	0.0	5.38923769232842E-4	0.015628789307752418	0.0
22	0.0	0.0	5.38923769232842E-4	0.019401255692382314	0.0
23	0.0	0.0	5.38923769232842E-4	0.025868340923176417	0.0
24	0.0	0.0	5.38923769232842E-4	0.02964080730780631	0.0
25	0.0	0.0	5.38923769232842E-4	0.03179650238473768	0.0
26	0.0	0.0	5.38923769232842E-4	0.03664681630783326	0.0
27	0.0	0.0	5.38923769232842E-4	0.07113793753873515	0.0
28	0.0	0.0	5.38923769232842E-4	0.10670690630810271	0.0
29	0.0	0.0	5.38923769232842E-4	0.13257524723127914	0.0
30	0.0	0.0	5.38923769232842E-4	0.17622807253913933	0.0
31	0.0	0.0	5.38923769232842E-4	0.20047964215461722	0.0
32	0.0	0.0	5.38923769232842E-4	0.24305461992401176	0.0
33	0.0	0.0	5.38923769232842E-4	0.28778529277033765	0.0
34	0.0	0.0	5.38923769232842E-4	0.3476058311551831	0.0
35	0.0	0.0	5.38923769232842E-4	0.46994152677103823	0.0
36	0.0	0.0	5.38923769232842E-4	0.5707202716175797	0.0
37	0.0	0.0	5.38923769232842E-4	0.8919188380803535	0.0
38	0.0	0.0	5.38923769232842E-4	1.1856322923122524	0.0
39	0.0	0.0	5.38923769232842E-4	1.5806634151599257	0.0
40	0.0	0.0	5.38923769232842E-4	2.0285090673924175	0.0
41	0.0	0.0	5.38923769232842E-4	2.5701274554714235	0.0
42	0.0	0.0	5.38923769232842E-4	3.195817951550753	0.0
43	0.0	0.0	5.38923769232842E-4	3.861388806553313	0.0
44	0.0	0.0	5.38923769232842E-4	4.494624235401902	0.0
45	0.0	0.0	5.38923769232842E-4	4.947859125326723	0.0
46	0.0	0.0	5.38923769232842E-4	5.4964835224057556	0.0
47	0.0	0.0	5.38923769232842E-4	6.1130123144081265	0.0
48	0.0	0.0	5.38923769232842E-4	6.544690253563633	0.0
49	0.0	0.0	5.38923769232842E-4	6.944571690334402	0.0
50	0.0	0.0	5.38923769232842E-4	7.54331599795209	0.0
51	0.0	0.0	5.38923769232842E-4	8.234216270108593	0.0
52	0.0	0.0	5.38923769232842E-4	8.88954757349573	0.0
53	0.0	0.0	5.38923769232842E-4	9.746436366575947	0.0
54	0.0	0.0	5.38923769232842E-4	10.495540405809598	0.0
55	0.0	0.0	5.38923769232842E-4	11.013985071811593	0.0
56	0.0	0.0	5.38923769232842E-4	11.490393683813425	0.0
57	0.0	0.0	5.38923769232842E-4	11.888658349276495	0.0
58	0.0	0.0	5.38923769232842E-4	12.280994853278004	0.0
59	0.0	0.0	5.38923769232842E-4	12.73746328581822	0.0
60	0.0	0.0	5.38923769232842E-4	13.044110910511709	0.0
61	0.0	0.0	5.38923769232842E-4	13.377704723666838	0.0
62	0.0	0.0	5.38923769232842E-4	13.605130554283097	0.0
63	0.0	0.0	5.38923769232842E-4	13.91501172159198	0.0
64	0.0	0.0	5.38923769232842E-4	14.28471342728571	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGTATA	20	7.912189E-5	69.998116	48
ATCTCGT	40	1.0608346E-8	61.248352	42
GACGCTT	50	9.749783E-10	55.99849	14
TCGCATA	20	0.0069436375	52.498585	40
CTTAGAT	20	0.0069436375	52.498585	3
CGACGAT	55	2.2700988E-9	50.90772	22
CGTGTAT	35	2.0809812E-5	49.998653	35
TAGATCT	140	0.0	49.998653	39
TCGTTGG	50	6.165101E-8	48.99868	45
TCGGTGG	100	0.0	48.99868	45
ATCTCGG	110	0.0	47.725986	42
GTAGATC	155	0.0	47.41808	38
AGATCTC	135	0.0	46.66541	40
GATCTCG	160	0.0	45.936264	41
TGGTCGC	155	0.0	45.160076	49
TCGTCAT	40	4.580579E-5	43.74882	8
TTCGGTG	40	4.580579E-5	43.74882	44
ACGCTTC	105	0.0	43.332165	15
GCAGTGT	65	9.949872E-9	43.07576	33
GCCGACT	65	9.949872E-9	43.07576	20
>>END_MODULE