FastQCFastQC Report
Wed 25 May 2016
SRR1779722_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779722_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences185555
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT2015110.859852873810999No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCG24361.3128183018512032No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC14150.7625771334644714No Hit
ATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTCTT8970.4834146210018593Illumina PCR Primer Index 10 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC6230.33574950823206057No Hit
CTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTC5260.28347390261647487Illumina PCR Primer Index 10 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTC3270.17622807253913933Illumina PCR Primer Index 10 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTACCTCGTATGCCGT3070.16544959715448251No Hit
CTTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCG2190.11802430546199238No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTGT150.002221956870.0000134
CTCAACG207.911134E-570.0000146
ACTGATA150.002221956870.000018
ACGCTTT150.002221956870.0000144
CATCGTT150.002221956870.0000132
TTTTGAC301.01956175E-770.0000131
CGCTTTC150.002221956870.0000145
GACAGAC200.00694289852.5000047
TGCTTTT200.00694289852.50000464
TTCCCTA200.00694289852.50000457
ATATAAG200.00694289852.5000045
ACAGATC200.00694289852.5000048
GCACTAT200.00694289852.50000444
TATGCCG30450.049.8850643
CTTGAAA30150.049.80099557
CTGCTTG30350.049.7034654
ATCTCGT30000.049.69999737
TCTCGTA30000.049.69999738
TCGTATG30450.049.65517440
CGTATGC30600.049.64052241
Produced by FastQC (version 0.11.5)