FastQCFastQC Report
Wed 25 May 2016
SRR1779721_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779721_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82778
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTCGA252.8177456E-670.0000136
CTTCCGG207.8852856E-570.042
GCAAACA150.002217595570.065
TTCAAAA207.8852856E-570.063
AGTGGCT150.002217595570.047
TGAAAGT150.002217595570.013
GTGTCTA150.002217595570.036
TTTAGAT401.0510121E-861.2537
TCGTTTA252.3780481E-456.00000434
TGTACAT252.3780481E-456.00000437
GCCGTAT750.056.054
CTACTAG200.00692937452.522
GTTTCCA200.00692937452.536
GCTGGTA200.00692937452.547
CATTGTA200.00692937452.534
TTAGATC407.3995034E-752.538
TTCCGGT200.00692937452.543
ACTTACA200.00692937452.569
AGTCTTG200.00692937452.548
GAAGTGT200.00692937452.533
Produced by FastQC (version 0.11.5)