##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779721_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	82778
Sequences flagged as poor quality	0
Sequence length	76
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.29910121046655	32.0	21.0	34.0	12.0	34.0
2	25.784532122061417	31.0	21.0	34.0	12.0	34.0
3	25.14971369204378	27.0	21.0	34.0	11.0	34.0
4	26.157264007344946	32.0	21.0	34.0	12.0	34.0
5	26.156877431201526	32.0	21.0	34.0	11.0	34.0
6	27.655174080069585	33.0	21.0	37.0	11.0	38.0
7	28.703326970934306	34.0	21.0	38.0	12.0	38.0
8	27.909239169827732	34.0	21.0	38.0	11.0	38.0
9	29.477179927033752	34.0	22.0	38.0	12.0	38.0
10-11	28.702390731836964	34.0	21.0	38.0	11.0	38.0
12-13	28.507187900166713	33.0	21.0	37.5	11.0	38.0
14-15	27.887778153615695	33.5	21.0	37.5	11.0	38.0
16-17	27.802248181884075	32.5	16.0	37.5	11.0	38.0
18-19	28.28643480151731	34.0	21.0	37.5	11.0	38.0
20-21	27.23671748532219	31.0	16.0	37.0	11.0	38.0
22-23	27.324929329048782	30.5	16.0	37.5	11.0	38.0
24-25	27.952275967044386	32.5	21.0	37.5	11.0	38.0
26-27	26.884268767063713	30.0	16.0	37.0	11.0	38.0
28-29	28.30401797579067	34.0	21.0	38.0	11.0	38.0
30-31	28.721061151513688	34.0	21.0	38.0	11.0	38.0
32-33	28.868986928894152	34.0	21.0	38.0	11.0	38.0
34-35	28.936607552731402	34.0	21.0	38.0	11.0	38.0
36-37	27.50791273043562	32.5	16.0	37.0	11.0	38.0
38-39	26.529047573026652	27.0	16.0	37.0	11.0	38.0
40-41	26.864867476865832	31.0	11.0	37.0	11.0	38.0
42-43	27.665418347870208	32.5	16.0	37.5	11.0	38.0
44-45	28.929812268960355	34.5	21.0	38.0	11.0	38.0
46-47	27.98627050665636	33.0	16.0	37.5	11.0	38.0
48-49	27.261289231438305	32.5	16.0	37.0	11.0	38.0
50-51	25.794202565899152	27.0	11.0	36.5	11.0	38.0
52-53	27.938347145376788	33.5	21.0	37.5	11.0	38.0
54-55	27.34608229239653	31.5	16.0	37.5	11.0	38.0
56-57	27.737484597356787	32.5	16.0	38.0	11.0	38.0
58-59	28.440890091570225	34.0	21.0	38.0	11.0	38.0
60-61	28.99541544854913	35.0	21.0	38.0	11.0	38.0
62-63	28.92708811519969	34.5	21.0	38.0	11.0	38.0
64-65	28.689990093986324	35.0	21.0	38.0	11.0	38.0
66-67	27.28235763125468	31.5	17.5	37.5	11.0	38.0
68-69	27.223072555509916	31.5	18.5	37.0	11.0	38.0
70-71	27.012243591292375	30.0	15.0	37.0	11.0	38.0
72-73	26.21589673584769	27.0	11.0	37.0	11.0	38.0
74-75	27.252639590229286	32.5	16.0	37.0	11.0	38.0
76	27.32154678779386	32.0	19.0	37.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	2.0
4	0.0
5	1.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	9.0
12	54.0
13	321.0
14	836.0
15	1491.0
16	2493.0
17	3957.0
18	5102.0
19	5246.0
20	4746.0
21	3630.0
22	2597.0
23	2056.0
24	1672.0
25	1596.0
26	1611.0
27	1634.0
28	1759.0
29	2075.0
30	2513.0
31	2933.0
32	3566.0
33	4676.0
34	5974.0
35	7579.0
36	8168.0
37	4481.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.59252458382662	17.221967189349826	12.921307593805118	35.26420063301843
2	17.372973495373166	14.360095677595497	43.72055377032545	24.546377056705886
3	17.76822064099931	14.180267465600348	42.68456213682545	25.36694975657489
4	14.610162120370147	22.141148614366134	27.945831017903306	35.302858247360405
5	14.290201384442538	24.27969121857521	41.68549234690796	19.744615050074295
6	25.150402280799245	32.17763173790137	15.761434197492088	26.91053178380729
7	23.505037570368938	22.560342119886926	19.319142767401967	34.61547754234216
8	20.351555421322864	32.291150709755364	16.584717607973424	30.77257626094835
9	20.19691935971006	14.715795832074901	18.214436726064633	46.87284808215041
10-11	23.260927344335176	21.80878053495059	26.73544832918549	28.19484379152874
12-13	21.49165196800928	16.46954357648537	30.797714258100367	31.24109019740498
14-15	24.816371895235335	20.65514158693341	22.083695757224316	32.44479076060694
16-17	27.81704178898916	18.446836528819784	23.078781728341365	30.657339953849682
18-19	21.5623263343562	17.948268780051706	27.993089617512744	32.49631526807935
20-21	27.50545155814895	17.955408972570055	24.112498414366744	30.426641054914256
22-23	29.038103752386014	16.787880252253125	23.639065407011863	30.534950588349
24-25	22.203563877982482	22.639081848384173	20.884929024463908	34.27242524916944
26-27	26.310925334187047	21.765498245253728	23.570380124553765	28.35319629600546
28-29	23.60797342192691	21.38205980066445	23.740863787375417	31.269102990033222
30-31	22.897148276341145	21.36950389310718	24.72560994025938	31.007737890292304
32-33	23.649652672908488	20.55209906372697	24.904862579281183	30.89338568408336
34-35	23.046813651464817	20.770764119601328	24.850498338870434	31.331923890063422
36-37	27.375911372601138	17.145584033537304	29.53421082834482	25.94429376551674
38-39	26.71430642472274	15.383453741513037	34.38700558146277	23.515234252301447
40-41	24.09558499296281	22.220007369419324	23.983835601543955	29.700572036073915
42-43	19.537903956508607	22.961643008154635	26.89096949562066	30.6094835397161
44-45	19.589970341107467	18.63798633637171	30.079311865369167	31.69273145715166
46-47	25.961945031712474	17.740259740259738	27.471458773784356	28.826336454243428
48-49	25.283905574214128	18.45144610626525	29.30630391185638	26.958344407664242
50-51	32.03160393599478	16.540722082283796	27.25960289702747	24.168071084693956
52-53	18.83841739655693	20.539414074297795	29.431591664149803	31.190576864995467
54-55	24.466619953125377	18.786092251189988	26.28361562809578	30.463672167588857
56-57	24.41241671750699	19.35898907285368	25.512983914322472	30.715610295316853
58-59	19.529079179452484	21.463865465001085	27.983424746901203	31.023630608645224
60-61	19.42083612706812	19.734338473805337	27.63532247250059	33.20950292662596
62-63	19.286853359750648	21.503733056273706	27.297218933481528	31.91219465049412
64-65	19.40440954394443	22.683177287828453	26.34732709151314	31.565086076713982
66-67	17.786489637383944	28.553220535557877	20.51199961340284	33.148290213655336
68-69	18.244325082019706	27.626896422551038	20.60613018023189	33.522648315197365
70-71	17.681987592647406	28.442073852138666	19.57631367527047	34.29962487994346
72-73	16.795530051344006	30.76472364844458	18.206584113560858	34.23316218665056
74-75	19.106241090197404	25.0616135501486	21.709715611182258	34.122429748471745
76	19.293497958296083	23.305627370913573	22.246115930122986	35.15475874066736
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	1.0
4	2.0
5	1.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.5
14	0.5
15	0.0
16	0.5
17	1.0
18	4.0
19	7.5
20	10.5
21	13.0
22	20.5
23	35.0
24	61.5
25	81.0
26	108.0
27	156.5
28	226.0
29	274.0
30	346.0
31	482.0
32	644.0
33	742.0
34	892.5
35	1169.5
36	1442.5
37	1589.0
38	1782.5
39	2155.5
40	2495.5
41	2869.0
42	3082.0
43	3207.0
44	3479.5
45	3661.0
46	3695.0
47	3709.5
48	3764.5
49	3664.5
50	3524.0
51	3431.0
52	3196.0
53	3002.5
54	2951.0
55	2853.5
56	2673.0
57	2508.5
58	2427.0
59	2428.0
60	2379.5
61	2369.0
62	2408.0
63	2380.0
64	2293.5
65	2167.5
66	2049.0
67	1998.0
68	1943.5
69	1786.5
70	1645.5
71	1607.0
72	1506.0
73	1307.5
74	1119.5
75	1029.0
76	942.0
77	748.0
78	565.5
79	490.0
80	407.0
81	273.5
82	174.5
83	126.0
84	100.0
85	62.5
86	37.5
87	24.0
88	16.5
89	7.0
90	3.5
91	1.0
92	0.0
93	0.5
94	0.5
95	0.0
96	0.0
97	0.5
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0012080504481867163
4	0.0
5	0.0012080504481867163
6	0.0
7	0.0
8	0.0036241513445601486
9	0.0036241513445601486
10-11	0.004832201792746865
12-13	0.004832201792746865
14-15	0.0024161008963734325
16-17	0.006040252240933581
18-19	0.004832201792746865
20-21	0.004228176568653506
22-23	0.004832201792746865
24-25	0.0036241513445601486
26-27	0.0030201261204667903
28-29	0.0036241513445601486
30-31	0.004228176568653506
32-33	0.0036241513445601486
34-35	0.0036241513445601486
36-37	0.005436227016840223
38-39	0.004832201792746865
40-41	0.004228176568653506
42-43	0.0036241513445601486
44-45	0.004228176568653506
46-47	0.0036241513445601486
48-49	0.004832201792746865
50-51	0.004228176568653506
52-53	0.0036241513445601486
54-55	0.004832201792746865
56-57	0.0030201261204667903
58-59	0.004832201792746865
60-61	0.004228176568653506
62-63	0.004832201792746865
64-65	0.0036241513445601486
66-67	0.005436227016840223
68-69	0.028389185532387833
70-71	0.005436227016840223
72-73	0.0036241513445601486
74-75	0.004832201792746865
76	0.004832201792746865
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	82778.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	98.12631375486241
#Duplication Level	Percentage of deduplicated	Percentage of total
1	98.3884668890886	96.54497571818598
2	1.3677718000172356	2.6842880958708832
3	0.19820995481797926	0.583488366474184
4	0.03816464968544942	0.14979825557515283
5	0.0061555886589434545	0.030201261204667908
6	0.001231117731788691	0.007248302689120297
7	0.0	0.0
8	0.0	0.0
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.012080504481867163	0.0
2	0.0	0.0	0.0	0.013288554930053879	0.0
3	0.0	0.0	0.0	0.01691270627461403	0.0
4	0.0	0.0	0.0	0.02295295851554761	0.0
5	0.0	0.0	0.0	0.026577109860107757	0.0
6	0.0	0.0	0.0	0.026577109860107757	0.0
7	0.0	0.0	0.0	0.028993210756481192	0.0
8	0.0	0.0	0.0	0.028993210756481192	0.0012080504481867163
9	0.0	0.0	0.0	0.028993210756481192	0.0012080504481867163
10	0.0	0.0	0.0	0.030201261204667908	0.0012080504481867163
11	0.0	0.0	0.0	0.030201261204667908	0.0012080504481867163
12	0.0	0.0	0.0	0.030201261204667908	0.0012080504481867163
13	0.0	0.0	0.0	0.03140931165285462	0.0012080504481867163
14	0.0	0.0	0.0	0.03261736210104134	0.0012080504481867163
15	0.0	0.0	0.0	0.03261736210104134	0.0012080504481867163
16	0.0	0.0	0.0	0.03261736210104134	0.0012080504481867163
17	0.0	0.0	0.0	0.03261736210104134	0.0012080504481867163
18	0.0	0.0	0.0	0.03261736210104134	0.0012080504481867163
19	0.0	0.0	0.0	0.03382541254922806	0.0012080504481867163
20	0.0	0.0	0.0	0.03503346299741477	0.0012080504481867163
21	0.0	0.0	0.0	0.04107371523834835	0.0012080504481867163
22	0.0	0.0	0.0	0.04348981613472178	0.0012080504481867163
23	0.0	0.0	0.0	0.04348981613472178	0.0012080504481867163
24	0.0	0.0	0.0	0.055570320616588946	0.0012080504481867163
25	0.0	0.0	0.0	0.06281862330570924	0.0012080504481867163
26	0.0	0.0	0.0	0.06765082509845612	0.0012080504481867163
27	0.0	0.0	0.0	0.1195969943704849	0.0012080504481867163
28	0.0	0.0	0.0	0.18120756722800743	0.0012080504481867163
29	0.0	0.0	0.0	0.21986518156998236	0.0012080504481867163
30	0.0	0.0	0.0	0.26335499770470416	0.0012080504481867163
31	0.0	0.0	0.0	0.2887240571166252	0.0012080504481867163
32	0.0	0.0	0.0	0.35275073087052117	0.0012080504481867163
33	0.0	0.0	0.0	0.4034888496943632	0.0012080504481867163
34	0.0	0.0	0.0	0.4953006837565537	0.0012080504481867163
35	0.0	0.0	0.0	0.6511391915726401	0.0012080504481867163
36	0.0	0.0	0.0	0.7743603372876852	0.0012080504481867163
37	0.0	0.0	0.0	1.1379835221918868	0.0012080504481867163
38	0.0	0.0	0.0	1.4677812945468602	0.0012080504481867163
39	0.0	0.0	0.0	1.8809345478267172	0.0012080504481867163
40	0.0	0.0	0.0	2.3170407596221216	0.0012080504481867163
41	0.0	0.0	0.0	2.690328348111817	0.0012080504481867163
42	0.0	0.0	0.0	3.1409311652854623	0.0012080504481867163
43	0.0	0.0	0.0	3.57220517528812	0.0012080504481867163
44	0.0	0.0	0.0	4.028848244702699	0.0012080504481867163
45	0.0	0.0	0.0	4.3888472782623404	0.0012080504481867163
46	0.0	0.0	0.0	4.754886564062915	0.0012080504481867163
47	0.0	0.0	0.0	5.19461692720288	0.0012080504481867163
48	0.0	0.0	0.0	5.473676580734011	0.0012080504481867163
49	0.0	0.0	0.0	5.758776486506076	0.0012080504481867163
50	0.0	0.0	0.0	6.164681437096813	0.0012080504481867163
51	0.0	0.0	0.0	6.659982120853367	0.0012080504481867163
52	0.0	0.0	0.0	7.0477663147213025	0.0012080504481867163
53	0.0	0.0	0.0	7.476624223827587	0.0012080504481867163
54	0.0	0.0	0.0	7.878905023073764	0.0012080504481867163
55	0.0	0.0	0.0	8.214743047669671	0.0012080504481867163
56	0.0	0.0	0.0	8.530044214646404	0.0012080504481867163
57	0.0	0.0	0.0	8.806687767281161	0.0012080504481867163
58	0.0	0.0	0.0	9.080915219019547	0.0012080504481867163
59	0.0	0.0	0.0	9.384135881514412	0.0012080504481867163
60	0.0	0.0	0.0	9.552054893812366	0.0012080504481867163
61	0.0	0.0	0.0	9.752591268211361	0.0012080504481867163
62	0.0	0.0	0.0	9.860107758099979	0.0012080504481867163
63	0.0	0.0	0.0	10.023194568605184	0.0012080504481867163
64	0.0	0.0	0.0	10.180241126869458	0.0012080504481867163
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTGTCGA	25	2.8177456E-6	70.00001	36
CTTCCGG	20	7.8852856E-5	70.0	42
GCAAACA	15	0.0022175955	70.0	65
TTCAAAA	20	7.8852856E-5	70.0	63
AGTGGCT	15	0.0022175955	70.0	47
TGAAAGT	15	0.0022175955	70.0	13
GTGTCTA	15	0.0022175955	70.0	36
TTTAGAT	40	1.0510121E-8	61.25	37
TCGTTTA	25	2.3780481E-4	56.000004	34
TGTACAT	25	2.3780481E-4	56.000004	37
GCCGTAT	75	0.0	56.0	54
CTACTAG	20	0.006929374	52.5	22
GTTTCCA	20	0.006929374	52.5	36
GCTGGTA	20	0.006929374	52.5	47
CATTGTA	20	0.006929374	52.5	34
TTAGATC	40	7.3995034E-7	52.5	38
TTCCGGT	20	0.006929374	52.5	43
ACTTACA	20	0.006929374	52.5	69
AGTCTTG	20	0.006929374	52.5	48
GAAGTGT	20	0.006929374	52.5	33
>>END_MODULE