FastQCFastQC Report
Wed 25 May 2016
SRR1779721_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779721_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82778
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT2192326.48408997559738No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG26183.1626760733528236No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC16882.039189156539177No Hit
ATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCTT9331.1271110681582062RNA PCR Primer, Index 24 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC6830.8250984561115272No Hit
CTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTC5390.6511391915726401No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCACCTCGTATGCCGT4080.4928845828601802No Hit
CTTTACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTC3370.4071130010389234RNA PCR Primer, Index 24 (95% over 21bp)
CTTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG2200.26577109860107756No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC2100.2536905941192104No Hit
TTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTC1930.23315373650003623No Hit
TATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCT1750.21140882843267536RNA PCR Primer, Index 24 (95% over 22bp)
CTTAATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG1710.20657662663992848No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTGATAGCACCTCGTATGCCG1510.18241561767619416No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCAACTCGTATGCCGT1400.16912706274614028No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCAGCTCGTATGCCGT1350.1630868105052067No Hit
CCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG940.11355674212955133No Hit
TCTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT830.10026818719949745No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATGCCG150.002217595570.054
TCTTTAT900.058.333331
CTTTATA1050.053.3333322
CTTTTGC200.00692937452.551
CGACCTT200.00692937452.537
CGTCTTT200.00692937452.548
TTGTACT200.00692937452.54
CATAATT200.00692937452.523
TTTGCTT200.00692937452.553
TAATGCC200.00692937452.553
TCTCGTA33250.048.84210638
CTCGTAT34400.048.73546639
CATCTCG33200.048.70481536
GTATGCC34650.048.68686742
ATGCCGT34450.048.6647344
ATCTCGT33300.048.66366237
TATGCCG34600.048.65606743
CTTGAAA34300.048.57143457
CGTATGC34750.048.5467641
GCTTGAA34400.048.5319856
Produced by FastQC (version 0.11.5)