##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779721_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	82778
Sequences flagged as poor quality	0
Sequence length	76
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.68351494358404	34.0	34.0	34.0	34.0	34.0
2	33.68793640822441	34.0	34.0	34.0	34.0	34.0
3	33.70633501655029	34.0	34.0	34.0	34.0	34.0
4	33.727910797554905	34.0	34.0	34.0	34.0	34.0
5	33.73849331948102	34.0	34.0	34.0	34.0	34.0
6	37.41187271980478	38.0	38.0	38.0	37.0	38.0
7	37.4087921911619	38.0	38.0	38.0	37.0	38.0
8	37.32013336876948	38.0	38.0	38.0	36.0	38.0
9	37.33795211288023	38.0	38.0	38.0	37.0	38.0
10-11	37.23941747807389	38.0	38.0	38.0	36.0	38.0
12-13	37.21409070042765	38.0	38.0	38.0	36.0	38.0
14-15	37.24225639662713	38.0	38.0	38.0	36.0	38.0
16-17	37.1380741259755	38.0	38.0	38.0	35.5	38.0
18-19	37.13091038681775	38.0	38.0	38.0	36.0	38.0
20-21	37.166988813452846	38.0	38.0	38.0	36.0	38.0
22-23	37.15521636183527	38.0	38.0	38.0	36.0	38.0
24-25	37.078082340718545	38.0	38.0	38.0	35.5	38.0
26-27	36.95430549179734	38.0	38.0	38.0	35.0	38.0
28-29	36.91510425475368	38.0	37.5	38.0	34.5	38.0
30-31	36.72770542897871	38.0	37.0	38.0	34.5	38.0
32-33	36.79923409601585	38.0	37.0	38.0	34.5	38.0
34-35	36.72803160259973	38.0	37.0	38.0	34.5	38.0
36-37	36.69876054024016	38.0	37.0	38.0	34.0	38.0
38-39	35.39839087680302	38.0	36.0	38.0	29.0	38.0
40-41	36.408194206190046	38.0	37.0	38.0	34.0	38.0
42-43	36.868413104931264	38.0	37.0	38.0	35.0	38.0
44-45	36.87120370146657	38.0	37.5	38.0	34.5	38.0
46-47	36.87872985575878	38.0	37.5	38.0	35.0	38.0
48-49	36.83156756626157	38.0	37.5	38.0	34.5	38.0
50-51	36.71064171639807	38.0	37.0	38.0	34.0	38.0
52-53	36.68791828746768	38.0	37.0	38.0	34.0	38.0
54-55	36.71198869264781	38.0	37.0	38.0	34.0	38.0
56-57	36.713027616033244	38.0	37.0	38.0	34.0	38.0
58-59	36.57183067964918	38.0	37.0	38.0	34.0	38.0
60-61	36.58525212012854	38.0	37.0	38.0	34.0	38.0
62-63	36.68214984657759	38.0	37.0	38.0	34.0	38.0
64-65	36.75623958056488	38.0	37.0	38.0	35.0	38.0
66-67	36.627558046824035	38.0	37.0	38.0	34.0	38.0
68-69	36.266387204329654	38.0	37.0	38.0	34.0	38.0
70-71	33.88023387856677	38.0	33.0	38.0	21.0	38.0
72-73	29.78286501244292	37.0	22.5	38.0	11.0	38.0
74-75	29.273889197612892	37.0	21.0	38.0	11.0	38.0
76	29.001135567421297	36.0	21.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	1.0
21	4.0
22	4.0
23	19.0
24	20.0
25	60.0
26	111.0
27	209.0
28	346.0
29	572.0
30	980.0
31	1566.0
32	2372.0
33	3713.0
34	6590.0
35	15556.0
36	16430.0
37	34225.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.85133731184614	11.762787213994057	11.587619899006983	49.798255575152815
2	14.57633670782092	11.446277996569137	52.19985986614801	21.77752542946193
3	14.338350769528136	13.841842035323396	49.525236173862616	22.29457102128585
4	11.34600980936964	38.00647515040228	26.424895503636233	24.222619536591846
5	11.953659184807558	25.400468723573898	49.67745053033414	12.9684215612844
6	22.247457053806567	50.09543598540675	13.431104882939913	14.226002077846772
7	20.144241223513493	21.66034453598782	14.865060764937542	43.33035347556114
8	17.764381840585663	50.4904684819638	11.917417671361957	19.827732006088574
9	16.111768827466236	14.37700838387011	11.373794969677933	58.13742781898572
10-11	19.3614245330885	30.716494720819544	28.677305564280363	21.244775181811594
12-13	18.68310420643166	13.017347604435962	31.792263644929815	36.50728454420257
14-15	18.54115827876972	12.638623788929426	15.523448259199304	53.29676967310155
16-17	34.81903404286163	28.267172437120973	12.47010075140738	24.443692768610017
18-19	35.59883060716616	17.236463794728067	12.691173983425546	34.47353161468023
20-21	19.6797458261857	14.145062697818261	12.426610935272658	53.74858054072338
22-23	19.165720360482254	30.065959554470993	12.47191282707966	38.29640725796709
24-25	34.90178549856242	30.283408635144603	12.4954698108193	22.319336055473677
26-27	36.197419604242675	31.804948174635772	13.589963516876466	18.40766870424509
28-29	34.7580274952282	17.530624078861536	13.86056681727029	33.85078160863998
30-31	36.29647974099399	14.119089613182245	30.214549759597958	19.36988088622581
32-33	21.592693710889364	30.456763874459398	29.73253763077146	18.218004783879774
34-35	32.24709463867212	31.782599241344318	17.61458358501051	18.355722534973058
36-37	21.015245596656115	29.369518471091354	14.734591316533379	34.880644615719156
38-39	17.178477373215106	16.719418202904155	30.471260479837635	35.63084394404311
40-41	17.313174998187925	12.998622822489066	33.087293725385976	36.60090845393704
42-43	33.191790089154125	13.044528739520162	31.86535069704511	21.898330474280606
44-45	18.349078257508033	28.79750658387494	34.052526033487155	18.800889125129867
46-47	32.71702626301675	14.315397811012588	19.781826089057482	33.18574983691319
48-49	31.769914711638357	12.735267824784364	16.7423711614197	38.75244630215758
50-51	18.923506245620818	13.15204522940878	32.767764381840585	35.156684143129816
52-53	15.257073135374133	13.025199932349175	49.1543646862693	22.563362246007394
54-55	15.098214501437578	13.080166227741671	36.49278793882432	35.328831331996426
56-57	31.379714416874048	13.025803957573267	20.666119017130153	34.928362608422525
58-59	17.357268839546737	14.40902172074706	45.987460436347824	22.246249003358383
60-61	31.165285462320906	30.2381067433376	19.752228853076907	18.844378941264587
62-63	15.9239169827732	50.719998067119285	14.084660175408924	19.27142477469859
64-65	14.511706008842928	53.1010655004953	13.225736306748171	19.1614921839136
66-67	14.944188069293773	53.0738843654111	12.946072627992944	19.035854937302183
68-69	14.974389330498441	53.19287733455749	12.953320930682066	18.879412404262002
70-71	15.585662857280921	49.74449733020851	14.13056609244002	20.53927372007055
72-73	16.908478098045375	40.89975597380946	17.15733649037184	25.03442943777332
74-75	17.61277150933823	37.53412742516127	18.97786851578922	25.875232549711274
76	17.5517649617048	37.95815313247481	18.514581168909615	25.975500736910774
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.5
14	2.5
15	5.0
16	4.5
17	3.0
18	5.0
19	9.5
20	12.0
21	12.0
22	14.5
23	34.0
24	71.0
25	91.0
26	105.0
27	134.5
28	169.0
29	188.0
30	245.0
31	303.0
32	399.0
33	494.0
34	556.0
35	688.0
36	841.5
37	925.0
38	1014.0
39	1438.0
40	6227.5
41	11006.5
42	11331.0
43	10086.0
44	7254.0
45	4551.0
46	3435.0
47	2831.5
48	2051.0
49	1717.5
50	1561.0
51	1525.0
52	1286.5
53	1045.5
54	1007.0
55	1119.0
56	1224.0
57	1216.5
58	1216.0
59	1216.0
60	1295.0
61	1409.5
62	1445.0
63	1545.5
64	1680.5
65	1638.0
66	1546.5
67	1532.0
68	1490.5
69	1459.5
70	1439.5
71	1409.0
72	1423.5
73	1392.0
74	1239.5
75	1133.0
76	1136.5
77	1003.0
78	829.5
79	793.0
80	667.0
81	454.0
82	291.0
83	215.0
84	190.0
85	127.0
86	61.5
87	34.0
88	25.5
89	14.0
90	6.5
91	1.5
92	1.0
93	1.5
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	82778.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.96433835076953
#Duplication Level	Percentage of deduplicated	Percentage of total
1	54.76080384136582	18.599144700282682
2	24.780366352480883	16.832974945033705
3	11.744620309443357	11.966947739737611
4	4.719900409034324	6.412331778975091
5	1.9704783923172684	3.3462997414772038
6	0.8429663880490841	1.7178477373215106
7	0.3841365818957852	0.9132861388291575
8	0.23119331317801886	0.6281862330570925
9	0.13515916770407257	0.41315325327985697
>10	0.3556820202738752	1.8712701442412234
>50	0.017784101013693758	0.42402570731353745
>100	0.035568202027387516	2.585227959119573
>500	0.010670460608216255	2.603348715842374
>1k	0.007113640405477503	5.201865229892
>5k	0.0	0.0
>10k+	0.0035568202027387516	26.48408997559738
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT	21923	26.48408997559738	No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG	2618	3.1626760733528236	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC	1688	2.039189156539177	No Hit
ATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCTT	933	1.1271110681582062	RNA PCR Primer, Index 24 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC	683	0.8250984561115272	No Hit
CTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTC	539	0.6511391915726401	No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCACCTCGTATGCCGT	408	0.4928845828601802	No Hit
CTTTACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTC	337	0.4071130010389234	RNA PCR Primer, Index 24 (95% over 21bp)
CTTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG	220	0.26577109860107756	No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC	210	0.2536905941192104	No Hit
TTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTC	193	0.23315373650003623	No Hit
TATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCT	175	0.21140882843267536	RNA PCR Primer, Index 24 (95% over 22bp)
CTTAATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG	171	0.20657662663992848	No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTGATAGCACCTCGTATGCCG	151	0.18241561767619416	No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCAACTCGTATGCCGT	140	0.16912706274614028	No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCAGCTCGTATGCCGT	135	0.1630868105052067	No Hit
CCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG	94	0.11355674212955133	No Hit
TCTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT	83	0.10026818719949745	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.018120756722800745	0.0
2	0.0	0.0	0.0	0.02174490806736089	0.0
3	0.0	0.0	0.0	0.026577109860107757	0.0
4	0.0	0.0	0.0	0.03503346299741477	0.0
5	0.0	0.0	0.0	0.04228176568653507	0.0
6	0.0	0.0	0.0	0.04228176568653507	0.0
7	0.0	0.0	0.0	0.0446978665829085	0.0
8	0.0	0.0	0.0	0.0446978665829085	0.0
9	0.0	0.0	0.0	0.04590591703109522	0.0
10	0.0	0.0	0.0	0.04711396747928193	0.0
11	0.0	0.0	0.0	0.04711396747928193	0.0
12	0.0	0.0	0.0	0.04832201792746865	0.0
13	0.0	0.0	0.0	0.0519461692720288	0.0
14	0.0	0.0	0.0	0.053154219720215515	0.0
15	0.0	0.0	0.0	0.053154219720215515	0.0
16	0.0	0.0	0.0	0.055570320616588946	0.0
17	0.0	0.0	0.0	0.056778371064775665	0.0
18	0.0	0.0	0.0	0.057986421512962384	0.0
19	0.0	0.0	0.0	0.06161057285752253	0.0
20	0.0	0.0	0.0	0.06281862330570924	0.0
21	0.0	0.0	0.0	0.07248302689120298	0.0
22	0.0	0.0	0.0	0.07731522868394984	0.0
23	0.0	0.0	0.0	0.0821474304766967	0.0
24	0.0	0.0	0.0	0.08697963226944357	0.0
25	0.0	0.0	0.0	0.09181183406219044	0.0
26	0.0	0.0	0.0	0.0966440358549373	0.0
27	0.0	0.0	0.0	0.16067070960883326	0.0
28	0.0	0.0	0.0	0.2234893329145425	0.0
29	0.0	0.0	0.0	0.2971804102539322	0.0
30	0.0	0.0	0.0	0.36241513445601486	0.0
31	0.0	0.0	0.0	0.4433545144845249	0.0
32	0.0	0.0	0.0	0.5557032061658895	0.0
33	0.0	0.0	0.0	0.6583874942617604	0.0
34	0.0	0.0	0.0	0.8891251298654231	0.0
35	0.0	0.0	0.0	1.1730169851893015	0.0
36	0.0	0.0	0.0	1.580129986228225	0.0
37	0.0	0.0	0.0	2.1660344535987823	0.0
38	0.0	0.0	0.0	2.801468989344995	0.0
39	0.0	0.0	0.0	3.4707289376404358	0.0
40	0.0	0.0	0.0	4.120660078764889	0.0
41	0.0	0.0	0.0	4.6727391335862185	0.0
42	0.0	0.0	0.0	5.281596559472324	0.0
43	0.0	0.0	0.0	5.912198893425789	0.0
44	0.0	0.0	0.0	6.517432167967335	0.0
45	0.0	0.0	0.0	7.1903162676073356	0.0
46	0.0	0.0	0.0	7.8390393582836015	0.0
47	0.0	0.0	0.0	8.46480949044432	0.0
48	0.0	0.0	0.0	9.039841503781197	0.0
49	0.0	0.0	0.0	9.623329870255382	0.0
50	0.0	0.0	0.0	10.165744521491218	0.0
51	0.0	0.0	0.0	10.773393896929136	0.0
52	0.0	0.0	0.0	11.28681533740849	0.0
53	0.0	0.0	0.0	11.754330860856749	0.0
54	0.0	0.0	0.0	12.213390031167702	0.0
55	0.0	0.0	0.0	12.76546908598903	0.0
56	0.0	0.0	0.0	13.235400710333664	0.0
57	0.0	0.0	0.0	13.677547174370002	0.0
58	0.0	0.0	0.0	14.141438546473701	0.0
59	0.0	0.0	0.0	14.586001111406413	0.0
60	0.0	0.0	0.0	15.001570465582642	0.0
61	0.0	0.0	0.0	15.426804223344368	0.0
62	0.0	0.0	0.0	15.748145642562033	0.0
63	0.0	0.0	0.0	16.13955398777453	0.0
64	0.0	0.0	0.0	16.499553021334172	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATGCCG	15	0.0022175955	70.0	54
TCTTTAT	90	0.0	58.33333	1
CTTTATA	105	0.0	53.333332	2
CTTTTGC	20	0.006929374	52.5	51
CGACCTT	20	0.006929374	52.5	37
CGTCTTT	20	0.006929374	52.5	48
TTGTACT	20	0.006929374	52.5	4
CATAATT	20	0.006929374	52.5	23
TTTGCTT	20	0.006929374	52.5	53
TAATGCC	20	0.006929374	52.5	53
TCTCGTA	3325	0.0	48.842106	38
CTCGTAT	3440	0.0	48.735466	39
CATCTCG	3320	0.0	48.704815	36
GTATGCC	3465	0.0	48.686867	42
ATGCCGT	3445	0.0	48.66473	44
ATCTCGT	3330	0.0	48.663662	37
TATGCCG	3460	0.0	48.656067	43
CTTGAAA	3430	0.0	48.571434	57
CGTATGC	3475	0.0	48.54676	41
GCTTGAA	3440	0.0	48.53198	56
>>END_MODULE