FastQCFastQC Report
Wed 25 May 2016
SRR1779720_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779720_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61739
Sequences flagged as poor quality0
Sequence length76
%GC54

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTACA252.8092363E-670.0000161
GTGTTTG150.002214912370.048
TTTAGAC150.002214912370.037
GTTTCGA150.002214912370.036
GTTTCAA150.002214912370.036
CCTAGGT150.002214912370.043
TCAGGTG150.002214912370.044
ATGTATC150.002214912370.028
TTAAGTA150.002214912370.02
AGTCTCA150.002214912370.056
GACGAAT150.002214912370.023
CGTTTCG150.002214912370.035
GTCTATA207.8693985E-569.9999936
CCGTATG207.8693985E-569.9999924
TTAAGGT207.8693985E-569.9999943
TCGGTGG1100.063.63636845
GTGTATA401.0450094E-861.24999636
GTTTAGA352.929719E-760.036
TTAGTGT352.929719E-760.033
CGTGTAT352.929719E-760.035