##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779720_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	61739
Sequences flagged as poor quality	0
Sequence length	76
%GC	54
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	22.928635060496607	23.0	12.0	33.0	12.0	34.0
2	23.636356273992128	23.0	12.0	33.0	12.0	34.0
3	23.42909668119017	23.0	11.0	32.0	11.0	34.0
4	23.04668038031066	23.0	12.0	33.0	11.0	34.0
5	23.325385898702603	23.0	11.0	33.0	11.0	34.0
6	24.225351884546235	26.0	12.0	34.0	11.0	38.0
7	25.233482887639905	27.0	12.0	36.0	12.0	38.0
8	24.5247898411053	26.0	11.0	36.0	11.0	38.0
9	26.00709438118531	28.0	12.0	37.0	12.0	38.0
10-11	24.981567566692043	26.5	11.0	36.0	11.0	38.0
12-13	25.42208328609145	27.0	11.0	35.5	11.0	38.0
14-15	24.245031503587683	26.0	11.0	35.0	11.0	38.0
16-17	24.32902217399051	26.0	11.0	35.0	11.0	38.0
18-19	24.76756183287711	26.0	11.5	35.0	11.0	38.0
20-21	23.89305787265748	24.5	11.0	34.0	11.0	37.5
22-23	24.07901812468618	24.5	11.0	35.0	11.0	37.5
24-25	24.114967848523627	25.0	11.0	35.0	11.0	38.0
26-27	23.33923451950955	23.0	11.0	34.0	10.0	38.0
28-29	24.02806167900355	25.5	11.0	35.5	11.0	38.0
30-31	24.423573430084712	26.0	11.0	36.0	11.0	38.0
32-33	24.667438734025495	26.0	11.0	37.0	11.0	38.0
34-35	24.76638753462155	26.0	11.0	37.0	11.0	38.0
36-37	24.09851957433713	24.5	11.0	34.0	11.0	38.0
38-39	24.02124265051264	25.0	11.0	34.0	11.0	37.0
40-41	23.336165146827774	24.5	11.0	34.0	11.0	37.0
42-43	23.849811302418246	25.5	11.0	34.5	11.0	38.0
44-45	24.70639304167544	26.0	11.0	36.5	11.0	38.0
46-47	24.227271254798424	25.0	11.0	35.5	11.0	38.0
48-49	23.524336319020392	24.0	11.0	34.0	11.0	38.0
50-51	22.859213787071383	23.0	11.0	33.5	11.0	37.0
52-53	23.644349600738593	24.0	11.0	35.0	11.0	37.5
54-55	23.42611639320365	24.0	11.0	34.5	11.0	38.0
56-57	23.803835501060917	24.0	11.0	35.0	11.0	38.0
58-59	24.001838384165602	24.5	11.0	36.0	11.0	38.0
60-61	24.610100584719547	25.5	11.0	37.0	11.0	38.0
62-63	24.64631756264274	25.5	11.0	36.5	11.0	38.0
64-65	24.55459272097054	25.5	11.0	36.5	11.0	38.0
66-67	23.811974602763247	24.5	11.0	34.0	11.0	38.0
68-69	23.57776284034403	24.0	11.0	34.0	11.0	38.0
70-71	23.498542250441375	24.0	11.0	34.0	11.0	38.0
72-73	23.223602585075884	24.0	11.0	34.0	11.0	37.0
74-75	23.152958421743143	23.5	11.0	34.0	11.0	37.5
76	22.826641183044753	23.0	11.0	34.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	fail
#Quality	Count
3	1.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	1.0
11	14.0
12	67.0
13	391.0
14	1119.0
15	1940.0
16	3028.0
17	4796.0
18	6350.0
19	6366.0
20	5415.0
21	4096.0
22	2865.0
23	2074.0
24	1526.0
25	1280.0
26	1082.0
27	1065.0
28	1087.0
29	1115.0
30	1270.0
31	1451.0
32	1651.0
33	2120.0
34	2456.0
35	2876.0
36	2904.0
37	1333.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.514844749672008	15.711300798522815	16.856444062909993	40.917410388895185
2	14.30214289185118	12.08312411927631	51.00341761285411	22.6113153760184
3	14.172565153306662	11.148544680023972	49.70925994914074	24.96963021752863
4	13.608901990638008	24.750967783734755	25.036038808532695	36.604091417094544
5	12.876787767861481	21.20053774761496	44.55206595506892	21.37060852945464
6	22.407230437810785	33.785775603751276	15.209187061662806	28.597806896775136
7	20.67250846304605	18.723983219682857	18.743419880464536	41.860088436806556
8	17.729761249149632	35.253814506462795	15.892967054326345	31.123457190061227
9	17.368557452460397	14.96323172114419	16.571641452589976	51.09656937380544
10-11	19.884187082405344	21.61328204089897	28.591212796112575	29.911318080583115
12-13	17.705751818196543	14.54881189562175	34.3999546463223	33.3454816398594
14-15	24.748129191097863	20.84858596002462	21.549126955845672	32.85415789303185
16-17	29.909130667184996	15.728007515752305	21.177737823347424	33.18512399371528
18-19	18.533306337315246	14.980360396841466	30.69528244583924	35.79105082000405
20-21	29.746912330431265	14.797327394209352	22.605385705608423	32.85037456975096
22-23	31.767307007029704	13.481162331141276	20.966017687647803	33.785512974181216
24-25	19.84515209433412	22.312838122388158	19.300916777349446	38.54109300592828
26-27	28.537529560400404	21.06239269169717	21.271340179468073	29.128737568434353
28-29	21.845540833846254	20.38047879749911	23.00690012634034	34.76708024231429
30-31	20.61162673923255	20.73472957869673	24.675640215753923	33.9780034663168
32-33	22.405163756519485	18.598756033561177	24.808869739868477	34.18721047005086
34-35	21.03323722828728	18.998833781463603	25.045352943082055	34.922576047167055
36-37	28.898157521765537	13.398663697104677	30.47094553553351	27.232233245596277
38-39	28.627657420530472	11.033002632111764	36.886819194168865	23.452520753188903
40-41	23.859080785584126	23.209556590402915	22.994128366066004	29.937234257946955
42-43	16.565296618748736	23.72949989876493	27.404737801174328	32.300465681312005
44-45	17.250455557805225	15.636363636363637	31.82020651953837	35.292974286292775
46-47	28.27594026369497	14.705691794356799	25.481065146263244	31.53730279568499
48-49	26.90909090909091	15.250050617533914	30.1056894108119	27.73516906256327
50-51	40.404130390767364	12.561247216035634	23.80643855031383	23.228183842883173
52-53	16.80029803845344	18.501870839205015	30.412070557364302	34.28576056497724
54-55	26.812122390138814	15.593566256863795	24.482887085540277	33.11142426745712
56-57	26.767145032232985	16.362693964819076	23.417506236029674	33.452654766918265
58-59	17.633529054464468	19.922251467908485	28.840656003239523	33.60356347438753
60-61	17.3346831342377	17.708847944928124	28.47135047580482	36.48511844502936
62-63	17.272322332455964	20.498076533711277	27.876088276979143	34.35351285685361
64-65	17.324964567726262	22.321117635148816	27.347236282648307	33.00668151447662
66-67	14.874386510520434	33.294458752449906	18.38200754814779	33.449147188881874
68-69	15.755066992855177	31.71913225215884	18.497156651491338	34.02864410349464
70-71	14.821623810487953	32.801781737193764	17.282850779510024	35.09374367280826
72-73	13.544551889466607	37.24670780893143	16.257673680288967	32.95106662131299
74-75	16.607679411693244	25.9230763000818	20.723558996703733	36.74568529152123
76	16.66909632797188	23.444611821112137	22.59261059008374	37.29368126083224
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.5
3	0.5
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	1.5
17	3.0
18	3.0
19	5.0
20	7.5
21	8.0
22	11.0
23	21.5
24	44.5
25	60.0
26	72.5
27	111.0
28	159.5
29	182.0
30	205.0
31	322.5
32	457.0
33	497.0
34	593.0
35	749.5
36	932.0
37	1054.0
38	1181.5
39	1435.5
40	1695.0
41	1940.5
42	2053.0
43	2194.0
44	2358.0
45	2469.5
46	2558.0
47	2571.5
48	2608.5
49	2579.0
50	2526.0
51	2463.5
52	2350.5
53	2261.5
54	2223.0
55	2154.0
56	2101.0
57	2049.5
58	1982.0
59	1913.5
60	1872.5
61	1862.5
62	1825.0
63	1835.0
64	1865.0
65	1793.0
66	1662.0
67	1623.0
68	1591.0
69	1528.5
70	1401.5
71	1305.0
72	1278.5
73	1143.5
74	977.5
75	920.0
76	804.5
77	617.0
78	494.0
79	443.0
80	369.5
81	255.0
82	162.0
83	110.0
84	92.5
85	55.5
86	26.0
87	16.0
88	13.5
89	8.0
90	4.0
91	1.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0016197217318064756
9	0.0016197217318064756
10-11	0.0024295825977097133
12-13	0.0032394434636129513
14-15	0.0016197217318064756
16-17	0.0032394434636129513
18-19	0.0024295825977097133
20-21	0.0024295825977097133
22-23	0.0016197217318064756
24-25	0.0016197217318064756
26-27	0.0016197217318064756
28-29	0.0016197217318064756
30-31	0.0032394434636129513
32-33	0.0016197217318064756
34-35	0.0016197217318064756
36-37	0.0024295825977097133
38-39	0.0024295825977097133
40-41	0.0024295825977097133
42-43	0.0024295825977097133
44-45	0.0024295825977097133
46-47	0.0016197217318064756
48-49	0.0024295825977097133
50-51	0.0024295825977097133
52-53	0.0032394434636129513
54-55	0.0032394434636129513
56-57	0.0016197217318064756
58-59	0.0024295825977097133
60-61	0.0024295825977097133
62-63	0.0024295825977097133
64-65	0.0024295825977097133
66-67	0.0032394434636129513
68-69	0.02591554770890361
70-71	0.0024295825977097133
72-73	0.0032394434636129513
74-75	0.004049304329516189
76	0.0032394434636129513
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	61739.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	99.29218160320058
#Duplication Level	Percentage of deduplicated	Percentage of total
1	99.37359303122247	98.67020845818688
2	0.551368633976053	1.0949318907011776
3	0.06851326221004209	0.20408493820761592
4	0.004893804443574435	0.019436660781677707
5	0.0	0.0
6	0.0	0.0
7	0.001631268147858145	0.01133805212264533
8	0.0	0.0
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0032394434636129513	0.0
6	0.0	0.0	0.0	0.0032394434636129513	0.0
7	0.0	0.0	0.0	0.0032394434636129513	0.0
8	0.0	0.0	0.0	0.0032394434636129513	0.0
9	0.0	0.0	0.0	0.0032394434636129513	0.0
10	0.0	0.0	0.0	0.004859165195419427	0.0
11	0.0	0.0	0.0	0.008098608659032377	0.0
12	0.0	0.0	0.0	0.008098608659032377	0.0
13	0.0	0.0	0.0	0.009718330390838853	0.0
14	0.0	0.0	0.0	0.017816939049871233	0.0
15	0.0	0.0	0.0	0.017816939049871233	0.0
16	0.0	0.0	0.0	0.021056382513484184	0.0
17	0.0	0.0	0.0	0.024295825977097136	0.0
18	0.0	0.0	0.0	0.02915499117251656	0.0
19	0.0	0.0	0.0	0.03887332156335541	0.0
20	0.0	0.0	0.0	0.04697193022238779	0.0
21	0.0	0.0	0.0	0.04859165195419427	0.0
22	0.0	0.0	0.0	0.0728874779312914	0.0
23	0.0	0.0	0.0	0.07612692139490436	0.0
24	0.0	0.0	0.0	0.08422553005393674	0.0
25	0.0016197217318064756	0.0	0.0	0.09718330390838854	0.0
26	0.0016197217318064756	0.0	0.0	0.10042274737200149	0.0
27	0.0016197217318064756	0.0	0.0	0.1392960689353569	0.0
28	0.0016197217318064756	0.0	0.0	0.19760605128039002	0.0
29	0.0016197217318064756	0.0	0.0	0.2251413207211001	0.0
30	0.0016197217318064756	0.0	0.0	0.27535269440710086	0.0
31	0.0016197217318064756	0.0	0.0	0.2980287986523915	0.0
32	0.0016197217318064756	0.0	0.0	0.3514796158020052	0.0
33	0.0016197217318064756	0.0	0.0	0.4373248675877484	0.0
34	0.0016197217318064756	0.0	0.0	0.5085926237872334	0.0
35	0.0016197217318064756	0.0	0.0	0.6300717536727191	0.0
36	0.0016197217318064756	0.0	0.0	0.7483114400945917	0.0032394434636129513
37	0.0016197217318064756	0.0	0.0	1.0576782908696285	0.0032394434636129513
38	0.0016197217318064756	0.0	0.0	1.2844393333225352	0.0032394434636129513
39	0.0016197217318064756	0.0	0.0	1.5500736973387972	0.0032394434636129513
40	0.0016197217318064756	0.0	0.0	1.8740180437000924	0.0032394434636129513
41	0.0016197217318064756	0.0	0.0	2.1542299033026127	0.0032394434636129513
42	0.0016197217318064756	0.0	0.0	2.4150051021234553	0.0032394434636129513
43	0.0016197217318064756	0.0	0.0	2.687118353066943	0.0032394434636129513
44	0.0016197217318064756	0.0	0.0	2.9576118822786244	0.0032394434636129513
45	0.0016197217318064756	0.0	0.0	3.1325418293137237	0.0032394434636129513
46	0.0016197217318064756	0.0	0.0	3.3414859327167594	0.0032394434636129513
47	0.0016197217318064756	0.0	0.0	3.592542801146763	0.0032394434636129513
48	0.0016197217318064756	0.0	0.0	3.7512755308637975	0.0032394434636129513
49	0.0016197217318064756	0.0	0.0	3.8711349390174767	0.0032394434636129513
50	0.0016197217318064756	0.0	0.0	4.078459320688705	0.0032394434636129513
51	0.0016197217318064756	0.0	0.0	4.323037302191484	0.0032394434636129513
52	0.0016197217318064756	0.0	0.0	4.520643353471874	0.0032394434636129513
53	0.0016197217318064756	0.0	0.0	4.723108569947683	0.0032394434636129513
54	0.0016197217318064756	0.0	0.0	4.967686551450461	0.0032394434636129513
55	0.0016197217318064756	0.0	0.0	5.1393770550219475	0.0032394434636129513
56	0.0016197217318064756	0.0	0.0	5.311067558593433	0.0032394434636129513
57	0.0016197217318064756	0.0	0.0	5.471420010042275	0.0032394434636129513
58	0.0016197217318064756	0.0	0.0	5.604237192050406	0.0032394434636129513
59	0.0016197217318064756	0.0	0.0	5.754871313108408	0.0032394434636129513
60	0.0016197217318064756	0.0	0.0	5.861772947407635	0.0032394434636129513
61	0.0016197217318064756	0.0	0.0	5.968674581706863	0.0032394434636129513
62	0.0016197217318064756	0.0	0.0	6.036702894442735	0.0032394434636129513
63	0.0016197217318064756	0.0	0.0	6.145224250473769	0.0032394434636129513
64	0.0016197217318064756	0.0	0.0	6.234308945723125	0.0032394434636129513
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATTACA	25	2.8092363E-6	70.00001	61
GTGTTTG	15	0.0022149123	70.0	48
TTTAGAC	15	0.0022149123	70.0	37
GTTTCGA	15	0.0022149123	70.0	36
GTTTCAA	15	0.0022149123	70.0	36
CCTAGGT	15	0.0022149123	70.0	43
TCAGGTG	15	0.0022149123	70.0	44
ATGTATC	15	0.0022149123	70.0	28
TTAAGTA	15	0.0022149123	70.0	2
AGTCTCA	15	0.0022149123	70.0	56
GACGAAT	15	0.0022149123	70.0	23
CGTTTCG	15	0.0022149123	70.0	35
GTCTATA	20	7.8693985E-5	69.99999	36
CCGTATG	20	7.8693985E-5	69.99999	24
TTAAGGT	20	7.8693985E-5	69.99999	43
TCGGTGG	110	0.0	63.636368	45
GTGTATA	40	1.0450094E-8	61.249996	36
GTTTAGA	35	2.929719E-7	60.0	36
TTAGTGT	35	2.929719E-7	60.0	33
CGTGTAT	35	2.929719E-7	60.0	35
>>END_MODULE