FastQCFastQC Report
Wed 25 May 2016
SRR1779720_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779720_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61739
Sequences flagged as poor quality0
Sequence length76
%GC49

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT2743944.443544599037885No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG30885.001700707818397No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC18322.9673302126694634No Hit
ATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCTT10461.6942289314695733TruSeq Adapter, Index 12 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC8981.4545101151622153No Hit
CTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTC8351.3524676460584073TruSeq Adapter, Index 12 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTC4840.7839453181943342RNA PCR Primer, Index 12 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAACCTCGTATGCCGT3960.6414098057953643No Hit
TTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTC3020.48915596300555564TruSeq Adapter, Index 12 (95% over 21bp)
CTTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG2750.4454234762467808No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC2510.4065501546834254No Hit
CTTAATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG2170.3514796158020052No Hit
TATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCT2010.3255640680931016RNA PCR Primer, Index 12 (95% over 22bp)
CCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG1140.18464827742593823No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACTTGAAACCTCGTATGCCG1100.17816939049871233No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAAGCTCGTATGCCGT1050.17007078183967994No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAAACTCGTATGCCGT1040.16845106010787347No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGAATCTCGTATGCCGTC970.15711300798522815Illumina PCR Primer Index 8 (95% over 22bp)
TCTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT960.15549328625342165No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATATCGTATGCCGT740.1198594081536792No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTACA650.070.01
CTCTATA150.002214912370.01
TCTATAC150.002214912370.02
AACCACG150.002214912370.069
ATAAAAA150.002214912370.070
AATACAA150.002214912370.070
AATAATT150.002214912370.070
TTTACAC600.070.02
AAAGACA150.002214912370.069
TCTTAAT353.6361598E-970.01
ACAAACA207.8693985E-569.9999970
TCTTTAT1000.066.500011
TTACACA650.064.615393
AAATCAT252.3732851E-456.00000469
GAAACCT750.056.034
CTATACA1150.054.782611
CTTAATA452.6479029E-854.4444472
TAATACA452.6479029E-854.4444474
TTAATAC452.6479029E-854.4444473
TTGAAAC850.053.5294132
Produced by FastQC (version 0.11.5)