##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779720_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	61739
Sequences flagged as poor quality	0
Sequence length	76
%GC	49
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.67054859975056	34.0	34.0	34.0	34.0	34.0
2	33.672135927047734	34.0	34.0	34.0	34.0	34.0
3	33.68775004454235	34.0	34.0	34.0	34.0	34.0
4	33.70208458186883	34.0	34.0	34.0	34.0	34.0
5	33.729393090267095	34.0	34.0	34.0	34.0	34.0
6	37.4266994930271	38.0	38.0	38.0	37.0	38.0
7	37.40175577835728	38.0	38.0	38.0	37.0	38.0
8	37.31178023615543	38.0	38.0	38.0	36.0	38.0
9	37.35526976465443	38.0	38.0	38.0	37.0	38.0
10-11	37.239913182915174	38.0	38.0	38.0	36.0	38.0
12-13	37.23349908485722	38.0	38.0	38.0	36.0	38.0
14-15	37.2579163899642	38.0	38.0	38.0	36.0	38.0
16-17	37.08483292570337	38.0	38.0	38.0	35.5	38.0
18-19	37.0472472829168	38.0	38.0	38.0	35.0	38.0
20-21	37.15619786520676	38.0	38.0	38.0	36.0	38.0
22-23	37.151468277749885	38.0	38.0	38.0	36.0	38.0
24-25	37.052851520108845	38.0	38.0	38.0	35.0	38.0
26-27	36.97797178444743	38.0	38.0	38.0	35.0	38.0
28-29	36.85402257892094	38.0	37.5	38.0	34.5	38.0
30-31	36.52350216232851	38.0	37.0	38.0	34.0	38.0
32-33	36.503555289201316	38.0	37.0	38.0	34.0	38.0
34-35	36.07349487358072	38.0	37.0	38.0	33.0	38.0
36-37	36.061330763374855	38.0	37.0	38.0	32.5	38.0
38-39	34.660733086055814	37.0	34.5	38.0	26.5	38.0
40-41	36.22286561168791	38.0	37.0	38.0	34.0	38.0
42-43	36.82783167851763	38.0	37.0	38.0	34.5	38.0
44-45	36.84598066052253	38.0	37.5	38.0	34.5	38.0
46-47	36.85691378221222	38.0	37.0	38.0	35.0	38.0
48-49	36.799786196731404	38.0	37.0	38.0	34.5	38.0
50-51	36.6141822834837	38.0	37.0	38.0	34.0	38.0
52-53	36.61235199792675	38.0	37.0	38.0	34.0	38.0
54-55	36.594113931226616	38.0	37.0	38.0	34.0	38.0
56-57	36.59296392879703	38.0	37.0	38.0	34.0	38.0
58-59	36.367968383031794	38.0	37.0	38.0	34.0	38.0
60-61	36.357699347252144	38.0	37.0	38.0	34.0	38.0
62-63	36.504357051458555	38.0	37.0	38.0	34.0	38.0
64-65	36.596745979040804	38.0	37.0	38.0	34.0	38.0
66-67	36.358282447075595	38.0	37.0	38.0	34.0	38.0
68-69	35.789120329127456	38.0	37.0	38.0	31.0	38.0
70-71	31.682040525437728	34.5	29.0	38.0	17.5	38.0
72-73	24.917993488718636	23.5	11.0	38.0	10.0	38.0
74-75	24.024895123017863	23.0	11.0	38.0	11.0	38.0
76	23.987981664749995	24.0	11.0	37.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	1.0
21	2.0
22	4.0
23	8.0
24	23.0
25	54.0
26	89.0
27	200.0
28	350.0
29	576.0
30	910.0
31	1464.0
32	2328.0
33	3608.0
34	6808.0
35	16587.0
36	15205.0
37	13522.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	16.73658465475631	6.299097814995385	11.657137303811204	65.3071802264371
2	9.956429485414406	5.852054617016797	65.18732081828342	19.00419507928538
3	9.669738738884659	8.738398743095937	64.80344676784529	16.78841575017412
4	8.0289606245647	52.498420771311494	21.441876285654125	18.030742318469688
5	9.321498566546268	20.186591943504105	60.96956542865936	9.522344061290271
6	15.440807269311133	60.77681854257438	10.874811707348679	12.907562480765804
7	13.775733329014075	18.2089117089684	9.821992581674468	58.19336238034306
8	11.840165859505337	61.74703185992646	7.015014820453846	19.39778746011435
9	9.935373102900922	13.733620563987106	5.291630897811756	71.03937543530022
10-11	12.901893454704483	35.4670467613664	31.832391195192667	19.798668588736454
12-13	12.733442394596608	7.49607217480037	37.16613485803139	42.60435057257163
14-15	13.842951780884045	5.936280147070733	11.247347705664167	68.97342036638105
16-17	41.26079139603816	30.74798749574823	5.067299437956559	22.923921670257048
18-19	42.272307617551306	12.351997926756184	5.03247542072272	40.34321903496979
20-21	15.297461896046261	7.186705324025333	5.095644568263173	72.42018821166523
22-23	14.84717925460406	33.77524741249454	4.972545716645881	46.40502761625553
24-25	41.276988613356224	34.53084760038225	4.909376569105428	19.28278721715609
26-27	41.8722363497951	37.88529130695347	6.471598179432774	13.770874163818656
28-29	14.856087724128994	13.275239313885875	31.472003109865724	40.3966698521194
30-31	12.945625941463257	32.529681400735356	14.422812160870762	40.101880496930626
32-33	13.187774340368325	10.566254717439543	58.08727060690973	18.1587003352824
34-35	39.234519509548264	33.41890863149711	15.65542039877549	11.691151460179142
36-37	14.344255656878149	63.70041626848507	8.385299405562124	13.570028669074652
38-39	12.445131926335055	14.426051604334376	34.533277182979965	38.59553928635061
40-41	12.867879298336545	6.168710215584962	37.577544177910234	43.38586630816826
42-43	39.23613923128006	6.699169082751583	35.86387858565898	18.200813100309368
44-45	14.878763828374286	32.4665122531949	38.828779215730734	13.825944702700077
46-47	38.78666645070377	8.173115858695477	14.191191953222438	38.84902573737832
48-49	38.34610213965241	5.551596235766695	9.739386773352338	46.36291485122856
50-51	15.799575632906265	6.073956494274284	37.17180388409271	40.954663988726736
52-53	10.791396038160645	5.967054859975057	63.414535382821235	19.82701371904307
54-55	11.301608383679685	5.7913150520740535	42.03987754903707	40.86719901520919
56-57	37.856136315780944	5.849625034419087	15.831160206676492	40.46307844312347
58-59	14.515946160449635	8.08160158084841	59.08177975023891	18.320672508463044
60-61	37.08595863230697	35.296166118660814	14.903059654351383	12.714815594680834
62-63	12.462948865384927	68.8325045757139	6.127407311423897	12.577139247477284
64-65	9.820372859942662	71.80064464924925	5.887688495116539	12.49129399569154
66-67	10.00259155477089	71.7083205105363	5.721667017606375	12.567420917086444
68-69	9.911077276923825	71.46860169422892	5.950047781791088	12.670273247056155
70-71	10.458543222274413	65.9842239103322	7.979559111744602	15.57767375564878
72-73	12.002138032685984	52.101588947018904	12.986118984758418	22.910154035536696
74-75	12.940871579322465	45.48377430614609	16.13012949780121	25.44522461673024
76	12.66136477753122	47.193832099645284	15.463483373556421	24.681319749267075
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	1.5
17	3.0
18	2.0
19	0.5
20	0.5
21	1.0
22	0.5
23	4.5
24	6.0
25	3.0
26	5.5
27	10.5
28	11.0
29	9.0
30	10.5
31	31.0
32	41.0
33	32.0
34	35.5
35	50.0
36	269.0
37	477.0
38	5822.5
39	11832.0
40	10768.0
41	6931.5
42	4823.0
43	3513.0
44	1502.5
45	582.5
46	363.0
47	272.5
48	155.5
49	147.0
50	165.0
51	158.0
52	185.5
53	241.5
54	263.0
55	315.5
56	424.5
57	586.5
58	692.0
59	710.5
60	835.0
61	915.0
62	889.0
63	951.0
64	1060.0
65	1111.0
66	1123.5
67	1132.0
68	1145.5
69	1215.5
70	1265.5
71	1259.0
72	1200.5
73	1123.0
74	1051.0
75	998.0
76	951.0
77	859.0
78	722.5
79	631.0
80	564.5
81	402.0
82	266.0
83	226.0
84	160.5
85	86.0
86	57.0
87	37.0
88	26.5
89	9.0
90	5.0
91	4.0
92	0.0
93	0.0
94	0.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	8.098608659032378E-4
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	61739.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.129269991415477
#Duplication Level	Percentage of deduplicated	Percentage of total
1	57.6466078957455	12.180307423184697
2	23.250287466462247	9.825232025138082
3	10.801073208125718	6.846563760345973
4	4.760444614794941	4.023388781807285
5	1.793790724415485	1.8950744262135766
6	0.7589114603296282	0.9621147086930465
7	0.22997316979685703	0.3401415636793599
8	0.11498658489842851	0.1943666078167771
9	0.030663089306247605	0.05830998234503312
>10	0.45228056726715216	2.097539642689386
>50	0.030663089306247605	0.5183109541780722
>100	0.08432349559218091	4.144867911692772
>500	0.015331544653123802	2.8069777612206224
>1k	0.022997316979685704	9.663259851957434
>5k	0.0	0.0
>10k+	0.007665772326561901	44.443544599037885
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT	27439	44.443544599037885	No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG	3088	5.001700707818397	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC	1832	2.9673302126694634	No Hit
ATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCTT	1046	1.6942289314695733	TruSeq Adapter, Index 12 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC	898	1.4545101151622153	No Hit
CTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTC	835	1.3524676460584073	TruSeq Adapter, Index 12 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTC	484	0.7839453181943342	RNA PCR Primer, Index 12 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAACCTCGTATGCCGT	396	0.6414098057953643	No Hit
TTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTC	302	0.48915596300555564	TruSeq Adapter, Index 12 (95% over 21bp)
CTTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG	275	0.4454234762467808	No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC	251	0.4065501546834254	No Hit
CTTAATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG	217	0.3514796158020052	No Hit
TATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCT	201	0.3255640680931016	RNA PCR Primer, Index 12 (95% over 22bp)
CCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG	114	0.18464827742593823	No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACTTGAAACCTCGTATGCCG	110	0.17816939049871233	No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAAGCTCGTATGCCGT	105	0.17007078183967994	No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAAACTCGTATGCCGT	104	0.16845106010787347	No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGAATCTCGTATGCCGTC	97	0.15711300798522815	Illumina PCR Primer Index 8 (95% over 22bp)
TCTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT	96	0.15549328625342165	No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATATCGTATGCCGT	74	0.1198594081536792	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.008098608659032377	0.0
6	0.0	0.0	0.0	0.008098608659032377	0.0
7	0.0	0.0	0.0	0.008098608659032377	0.0
8	0.0	0.0	0.0	0.008098608659032377	0.0
9	0.0	0.0	0.0	0.008098608659032377	0.0
10	0.0	0.0	0.0	0.009718330390838853	0.0
11	0.0	0.0	0.0	0.012957773854451805	0.0
12	0.0	0.0	0.0	0.012957773854451805	0.0
13	0.0	0.0	0.0	0.01457749558625828	0.0
14	0.0	0.0	0.0	0.024295825977097136	0.0
15	0.0	0.0	0.0	0.024295825977097136	0.0
16	0.0	0.0	0.0	0.027535269440710088	0.0
17	0.0	0.0	0.0	0.030774712904323036	0.0
18	0.0	0.0	0.0	0.03887332156335541	0.0
19	0.0	0.0	0.0	0.055070538881420175	0.0
20	0.0	0.0	0.0	0.06478886927225902	0.0
21	0.0	0.0	0.0	0.06964803446767845	0.0
22	0.0	0.0	0.0	0.07126775619948493	0.0
23	0.0	0.0	0.0	0.07450719966309788	0.0
24	0.0	0.0	0.0	0.08098608659032379	0.0
25	0.0	0.0	0.0	0.10366219083561444	0.0
26	0.0	0.0	0.0	0.10852135603103387	0.0
27	0.0	0.0	0.0	0.15711300798522815	0.0
28	0.0	0.0	0.0	0.21542299033026127	0.0
29	0.0	0.0	0.0	0.2818315813343268	0.0
30	0.0	0.0	0.0	0.3514796158020052	0.0
31	0.0	0.0	0.0	0.4195079285378772	0.0
32	0.0	0.0	0.0	0.5085926237872334	0.0
33	0.0	0.0	0.0	0.6867620142859456	0.0
34	0.0	0.0	0.0	0.8438750222711738	0.0
35	0.0	0.0	0.0	1.035002186624338	0.0
36	0.0	0.0	0.0	1.3783831937673108	0.0
37	0.0	0.0	0.0	1.8772574871637053	0.0
38	0.0	0.0	0.0	2.3145823547514537	0.0
39	0.0	0.0	0.0	2.7065550138486207	0.0
40	0.0	0.0	0.0	3.19409125512237	0.0
41	0.0	0.0	0.0	3.5876836359513438	0.0
42	0.0	0.0	0.0	3.968318242925865	0.0
43	0.0	0.0	0.0	4.352192293364	0.0
44	0.0	0.0	0.0	4.732826900338522	0.0
45	0.0	0.0	0.0	5.0486726380407845	0.0
46	0.0	0.0	0.0	5.414729749429048	0.0
47	0.0	0.0	0.0	5.764589643499247	0.0
48	0.0	0.0	0.0	6.0626184421516385	0.0
49	0.0	0.0	0.0	6.329872527899707	0.0
50	0.0	0.0	0.0	6.624661883088486	0.0
51	0.0	0.0	0.0	6.901634299227393	0.0
52	0.0	0.0	0.0	7.128395341680299	0.0
53	0.0	0.0	0.0	7.3260013929606895	0.0
54	0.0	0.0	0.0	7.602973809099597	0.0
55	0.0	0.0	0.0	7.828115129820697	0.0
56	0.0	0.0	0.0	8.050017007078184	0.0
57	0.0	0.0	0.0	8.292975266849155	0.0
58	0.0	0.0	0.0	8.485722152934127	0.0
59	0.0	0.0	0.0	8.70924375192342	0.0
60	0.0	0.0	0.0	8.892272307617551	0.0
61	0.0	0.0	0.0	9.128751680461297	0.0
62	0.0	0.0	0.0	9.326357731741687	0.0
63	0.0	0.0	0.0	9.567696269780852	0.0
64	0.0	0.0	0.0	9.758823434134015	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTTACA	65	0.0	70.0	1
CTCTATA	15	0.0022149123	70.0	1
TCTATAC	15	0.0022149123	70.0	2
AACCACG	15	0.0022149123	70.0	69
ATAAAAA	15	0.0022149123	70.0	70
AATACAA	15	0.0022149123	70.0	70
AATAATT	15	0.0022149123	70.0	70
TTTACAC	60	0.0	70.0	2
AAAGACA	15	0.0022149123	70.0	69
TCTTAAT	35	3.6361598E-9	70.0	1
ACAAACA	20	7.8693985E-5	69.99999	70
TCTTTAT	100	0.0	66.50001	1
TTACACA	65	0.0	64.61539	3
AAATCAT	25	2.3732851E-4	56.000004	69
GAAACCT	75	0.0	56.0	34
CTATACA	115	0.0	54.78261	1
CTTAATA	45	2.6479029E-8	54.444447	2
TAATACA	45	2.6479029E-8	54.444447	4
TTAATAC	45	2.6479029E-8	54.444447	3
TTGAAAC	85	0.0	53.52941	32
>>END_MODULE