FastQCFastQC Report
Wed 25 May 2016
SRR1779719_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779719_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences225758
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAATCG200.00694058152.5081466
GCGTATA200.00694058152.5081436
TCGTTTA506.164191E-849.007634
AGTGTCG305.864828E-446.67390435
ATCTCGG1850.045.4023942
CTTCCGA802.910383E-1143.7470918
CGTGTAT659.949872E-943.083635
TCTCGGT2000.041.99720843
CTCGTTT602.5842928E-740.83966433
GTCGATT350.001252670940.00620338
CGCCGTA1050.039.99734553
TACGGGG350.001254036639.9973430
ACCGTAG350.001254036639.9973414
CTCGGTG1850.039.72709344
TCGGTGG2050.039.26568245
GTGTCGA901.03682396E-1038.89492436
TCCGACT556.678374E-638.1792820
TGTCGAT753.5157427E-837.3391237
CTCGGTT753.522655E-837.33085344
ACGCTTC852.561137E-937.05636215