##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779719_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	225758
Sequences flagged as poor quality	0
Sequence length	76
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.331093471770657	34.0	24.0	34.0	12.0	34.0
2	27.881248947988553	34.0	23.0	34.0	12.0	34.0
3	26.75712931546169	32.0	21.0	34.0	11.0	34.0
4	29.086287086171918	34.0	23.0	34.0	12.0	34.0
5	28.716559324586505	34.0	23.0	34.0	11.0	34.0
6	30.941295546558706	36.0	26.0	38.0	11.0	38.0
7	31.9990786594495	37.0	28.0	38.0	12.0	38.0
8	31.173996048866485	37.0	27.0	38.0	11.0	38.0
9	32.64371583731252	37.0	31.0	38.0	12.0	38.0
10-11	32.352078331664885	37.0	31.0	38.0	11.0	38.0
12-13	31.411057415462572	36.0	28.5	38.0	11.0	38.0
14-15	31.382646462140876	36.5	27.0	38.0	11.0	38.0
16-17	31.061390958459942	36.0	27.0	38.0	11.0	38.0
18-19	31.66506834752257	37.0	27.5	38.0	11.5	38.0
20-21	30.541170191089577	35.5	25.0	38.0	11.0	38.0
22-23	30.529119676822084	35.5	26.0	38.0	11.0	38.0
24-25	31.666855659600103	36.5	29.0	38.0	11.0	38.0
26-27	30.277166257674146	35.5	24.5	38.0	11.0	38.0
28-29	32.31591350029678	37.0	31.0	38.0	11.0	38.0
30-31	32.7489014785744	37.5	32.5	38.0	11.0	38.0
32-33	32.86552857484563	38.0	34.0	38.0	11.0	38.0
34-35	32.910718557038955	38.0	34.0	38.0	11.0	38.0
36-37	30.869847358676104	36.5	26.5	38.0	11.0	38.0
38-39	29.170297398098846	34.0	23.0	37.5	11.0	38.0
40-41	30.315778399879516	35.0	25.0	38.0	11.0	38.0
42-43	31.31191364204148	36.5	28.0	38.0	11.0	38.0
44-45	32.935814013235415	37.5	33.5	38.0	11.0	38.0
46-47	31.537356372753123	37.0	28.5	38.0	11.0	38.0
48-49	31.02504008717299	36.5	27.0	38.0	11.0	38.0
50-51	28.881791564418535	34.0	22.0	37.5	11.0	38.0
52-53	32.09495787524695	37.0	29.5	38.0	11.0	38.0
54-55	31.202269687009984	36.5	27.0	38.0	11.0	38.0
56-57	31.63658652185083	37.0	28.5	38.0	11.0	38.0
58-59	32.65597453910825	37.0	32.5	38.0	11.0	38.0
60-61	33.12562788472612	38.0	34.0	38.0	11.0	38.0
62-63	33.0129209153164	38.0	34.0	38.0	11.0	38.0
64-65	32.74984496673429	37.5	32.5	38.0	11.0	38.0
66-67	30.697047280716518	36.5	25.0	38.0	11.0	38.0
68-69	30.768316072963085	36.5	25.5	38.0	11.0	38.0
70-71	30.395328183275897	35.5	25.0	38.0	11.0	38.0
72-73	29.208143233019427	34.0	22.5	37.5	11.0	38.0
74-75	31.257499180538453	36.5	28.0	38.0	11.0	38.0
76	31.619464205033708	37.0	27.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	4.0
4	2.0
5	1.0
6	3.0
7	2.0
8	0.0
9	0.0
10	3.0
11	16.0
12	73.0
13	386.0
14	994.0
15	1714.0
16	2965.0
17	4707.0
18	5957.0
19	6311.0
20	5647.0
21	4623.0
22	3731.0
23	3428.0
24	3263.0
25	3353.0
26	3772.0
27	4496.0
28	5440.0
29	6353.0
30	7857.0
31	10014.0
32	13052.0
33	17135.0
34	23119.0
35	30732.0
36	35864.0
37	20741.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.1330539781536	18.342650094348816	11.583199709423365	30.94109621807422
2	20.298284003224694	17.171484509961996	38.49210216249258	24.038129324320735
3	19.725989776663507	17.450544388238733	35.61601360749121	27.207452227606556
4	14.343741279340176	18.2373082562224	33.2344069065411	34.184543557896326
5	14.420308471903542	29.469609050399097	39.07148362405762	17.038598853639737
6	27.988376934593678	32.69474392934027	17.34157815005448	21.97530098601157
7	28.13898067842557	25.38381807067745	20.67612221936764	25.80107903152934
8	23.74464013607853	31.56251107409901	18.792303058223183	25.90054573159928
9	23.331768166273896	15.549196449137979	20.15388840654181	40.96514697804632
10-11	25.013178703215605	23.19673256756577	27.135060666332954	24.65502806288567
12-13	24.229637898803034	19.733274858464238	29.059573487848745	26.97751375488398
14-15	23.661560678278434	22.468859081808034	23.590685188794584	30.278895051118948
16-17	24.887979285858258	22.93750096903732	26.263680038274206	25.91083970683022
18-19	22.272550079294064	22.83181685286743	28.06123805051785	26.834395017320656
20-21	24.241296672824003	22.660987742915744	27.369577447319344	25.72813813694091
22-23	25.04994662024178	21.609720874807856	27.56986989514532	25.77046260980504
24-25	22.54332827606064	24.86162637459994	24.167045039258465	28.428000310080954
26-27	23.459696745529364	23.816062830286466	26.970201682384577	25.754038741799594
28-29	23.311288200612186	24.43753903671777	26.096461113891976	26.15471164877807
30-31	22.964029414370515	24.432311508815452	26.23150527155134	26.372153805262695
32-33	23.2220654583792	24.012553904490215	26.105388123403905	26.65999251372668
34-35	23.26927847473244	23.887899567651854	26.520527677369053	26.32229428024665
36-37	24.281809684549994	21.88326444908103	29.932090315892996	23.902835550475988
38-39	23.798238250388167	21.107992991984194	32.909323274630495	22.184445482997145
40-41	23.198164276031928	23.003030007707913	26.01000256930478	27.788803146955377
42-43	21.081016431857293	23.358973734277445	27.356750040420728	28.203259793444534
44-45	20.96561989182293	21.935315250663372	29.718571283019035	27.380493574494665
46-47	23.094301509674676	21.07940853356014	30.05949216811964	25.766797788645547
48-49	22.438695930486247	21.939004914957287	30.542062864496465	25.080236290060004
50-51	24.510348951815637	20.627692443991627	31.73700705434169	23.12495154985105
52-53	19.58917702509945	23.64269830160094	29.484765794579655	27.283358878719955
54-55	21.413699770976475	22.833025458379293	28.894175183063776	26.85909958758046
56-57	21.46709458217329	22.98495429587744	28.21163655532447	27.336314566624804
58-59	19.601228828294058	23.660481918637746	28.99219689603466	27.746092357033536
60-61	19.514707185257375	23.03357845308762	28.944803756534064	28.506910605120932
62-63	19.741117527061057	23.915841046870398	28.057783418497873	28.285258007570675
64-65	20.13196130198274	23.906081116997715	27.200905434378157	28.761052146641386
66-67	19.164252363359292	24.502742116967458	23.715989332766306	32.617016186906945
68-69	19.58518505390113	24.830631261326328	23.585787647713026	31.998396037059518
70-71	19.543587948914453	24.73719649683927	22.778075565143816	32.94113998910246
72-73	19.064779273821838	25.36241353571642	21.45465982409239	34.11814736636935
74-75	20.40700610420561	25.39181203653664	23.05821587093459	31.142965988323162
76	20.38751517191888	24.357463698138616	23.35722449124237	31.897796638700132
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.5
2	1.0
3	1.0
4	1.0
5	1.0
6	0.5
7	0.0
8	0.0
9	1.0
10	1.0
11	1.0
12	2.0
13	3.5
14	3.0
15	5.0
16	8.0
17	7.0
18	10.5
19	26.5
20	49.5
21	60.0
22	82.0
23	131.5
24	207.5
25	256.0
26	325.5
27	492.0
28	754.0
29	919.0
30	1136.0
31	1607.5
32	2219.5
33	2577.0
34	2996.0
35	3998.0
36	5102.5
37	5624.0
38	6412.0
39	7857.5
40	9177.5
41	10463.0
42	11086.0
43	11635.5
44	12561.0
45	13047.5
46	13158.0
47	13076.5
48	12890.5
49	12350.0
50	11914.0
51	11359.5
52	10298.0
53	9060.5
54	8330.0
55	7822.5
56	6778.5
57	5835.0
58	5428.0
59	5052.5
60	4494.0
61	4172.5
62	4034.0
63	3864.5
64	3582.0
65	3355.5
66	3115.0
67	2988.0
68	2858.0
69	2585.5
70	2345.5
71	2248.0
72	2098.5
73	1869.5
74	1638.5
75	1487.0
76	1341.0
77	1070.5
78	806.0
79	666.0
80	597.0
81	444.5
82	296.0
83	231.0
84	179.5
85	93.0
86	47.5
87	37.0
88	29.5
89	14.5
90	6.5
91	3.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	1.0
98	1.5
99	1.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	4.4295218774085523E-4
5	0.0
6	0.0
7	0.0
8	0.0026577131264451316
9	0.0044295218774085526
10-11	0.006644282816112828
12-13	0.008859043754817105
14-15	0.0044295218774085526
16-17	0.008194615473205822
18-19	0.007087235003853684
20-21	0.0048724740651494075
22-23	0.0075301871915945395
24-25	0.0046509979712789805
26-27	0.0031006653141859865
28-29	0.003986569689667698
30-31	0.007973139379335395
32-33	0.005093950159019835
34-35	0.006201330628371973
36-37	0.0075301871915945395
38-39	0.0073087110977241125
40-41	0.007087235003853684
42-43	0.003322141408056414
44-45	0.0075301871915945395
46-47	0.006201330628371973
48-49	0.008194615473205822
50-51	0.0046509979712789805
52-53	0.007087235003853684
54-55	0.0075301871915945395
56-57	0.00553690234676069
58-59	0.006422806722242401
60-61	0.007973139379335395
62-63	0.008194615473205822
64-65	0.0044295218774085526
66-67	0.008859043754817105
68-69	0.029677796578637304
70-71	0.00841609156707625
72-73	0.006422806722242401
74-75	0.005315426252890263
76	0.005315426252890263
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	225758.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	94.37372972523906
#Duplication Level	Percentage of deduplicated	Percentage of total
1	96.50651673571934	91.07679927141037
2	2.406628140075322	4.542449472812464
3	0.5567030115085025	1.576144186359902
4	0.23511842972155217	0.8875601255985752
5	0.12356237344756389	0.5830521017974725
6	0.05489361175577302	0.3108308927688917
7	0.03960678025304139	0.2616487702421224
8	0.025292933090130316	0.19095907451252112
9	0.01493441953825067	0.12684751857955784
>10	0.036743564890497384	0.4437085859181184
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	4.4295218774085523E-4	0.0
2	0.0	0.0	0.0	4.4295218774085523E-4	0.0
3	0.0	0.0	0.0	4.4295218774085523E-4	0.0
4	0.0	0.0	0.0	0.003986569689667698	0.0
5	0.0	0.0	0.0	0.0048724740651494075	0.0
6	0.0	0.0	0.0	0.0048724740651494075	0.0
7	0.0	0.0	0.0	0.005315426252890263	0.0
8	0.0	0.0	0.0	0.006201330628371974	0.0
9	0.0	0.0	0.0	0.006644282816112829	0.0
10	0.0	0.0	0.0	0.0075301871915945395	0.0
11	0.0	0.0	0.0	0.00841609156707625	0.0
12	0.0	0.0	0.0	0.008859043754817105	0.0
13	0.0	0.0	0.0	0.009301995942557961	0.0
14	0.0	0.0	0.0	0.011516756881262236	0.0
15	0.0	0.0	0.0	0.012402661256743948	0.0
16	0.0	0.0	0.0	0.012845613444484802	0.0
17	0.0	0.0	0.0	0.013731517819966513	0.0
18	0.0	0.0	0.0	0.014617422195448223	0.0
19	0.0	0.0	0.0	0.017275135321893356	0.0
20	0.0	0.0	0.0	0.019046944072856776	0.0
21	0.0	0.0	0.0	0.02037580063607934	0.0
22	0.0	0.0	0.0	0.028348940015414735	0.0
23	0.0	0.0	0.0	0.032335509705082435	0.0
24	0.0	0.0	0.0	0.035879127207009275	0.0
25	0.0	0.0	0.0	0.0376509359579727	0.0
26	0.0	0.0	0.0	0.04562407533730809	0.0
27	0.0	0.0	0.0	0.0930199594255796	0.0
28	0.0	0.0	0.0	0.13244270413451573	0.0
29	0.0	0.0	0.0	0.162120500713153	0.0
30	0.0	0.0	0.0	0.21261705011561052	0.0
31	0.0	0.0	0.0	0.23033513762524474	0.0
32	0.0	0.0	0.0	0.2741874042115894	0.0
33	0.0	0.0	0.0	0.3286705233037146	0.0
34	0.0	0.0	0.0	0.38448249895906234	0.0
35	0.0	0.0	0.0	0.5138245377793921	0.0
36	0.0	0.0	0.0	0.6254484890900877	0.0
37	0.0	0.0	0.0	0.960763295209915	0.0
38	0.0	0.0	0.0	1.291205627264593	0.0
39	0.0	0.0	0.0	1.7372584803196343	0.0
40	0.0	0.0	0.0	2.3343580293943074	0.0
41	0.0	0.0	0.0	3.078517704798944	0.0
42	0.0	0.0	0.0	3.8532410811577	0.0
43	0.0	0.0	0.0	4.707252899122069	0.0
44	0.0	0.0	0.0	5.4788756101666385	4.4295218774085523E-4
45	0.0	0.0	0.0	6.0272504185898175	4.4295218774085523E-4
46	0.0	0.0	0.0	6.655356620806351	4.4295218774085523E-4
47	0.0	0.0	0.0	7.342818416180157	4.4295218774085523E-4
48	0.0	0.0	0.0	7.798616217365497	4.4295218774085523E-4
49	0.0	0.0	0.0	8.216320130405125	4.4295218774085523E-4
50	0.0	0.0	0.0	8.841768619495213	4.4295218774085523E-4
51	0.0	0.0	0.0	9.601874573658518	4.4295218774085523E-4
52	0.0	0.0	0.0	10.279591420902028	4.4295218774085523E-4
53	0.0	0.0	0.0	11.210676919533306	4.4295218774085523E-4
54	0.0	0.0	0.0	12.09303767751309	4.4295218774085523E-4
55	0.0	0.0	0.0	12.593573649660255	4.4295218774085523E-4
56	0.0	0.0	0.0	13.17561282435174	4.4295218774085523E-4
57	0.0	0.0	0.0	13.599518068019737	4.4295218774085523E-4
58	0.0	0.0	0.0	13.979128092913651	4.4295218774085523E-4
59	0.0	0.0	0.0	14.468147308179555	4.4295218774085523E-4
60	0.0	0.0	0.0	14.754737373647888	4.4295218774085523E-4
61	0.0	0.0	0.0	15.075434757572268	4.4295218774085523E-4
62	0.0	0.0	0.0	15.323487982707146	4.4295218774085523E-4
63	0.0	0.0	0.0	15.653930314761825	4.4295218774085523E-4
64	0.0	0.0	0.0	16.006520256203544	4.4295218774085523E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCAATCG	20	0.006940581	52.50814	66
GCGTATA	20	0.006940581	52.50814	36
TCGTTTA	50	6.164191E-8	49.0076	34
AGTGTCG	30	5.864828E-4	46.673904	35
ATCTCGG	185	0.0	45.40239	42
CTTCCGA	80	2.910383E-11	43.74709	18
CGTGTAT	65	9.949872E-9	43.0836	35
TCTCGGT	200	0.0	41.997208	43
CTCGTTT	60	2.5842928E-7	40.839664	33
GTCGATT	35	0.0012526709	40.006203	38
CGCCGTA	105	0.0	39.997345	53
TACGGGG	35	0.0012540366	39.99734	30
ACCGTAG	35	0.0012540366	39.99734	14
CTCGGTG	185	0.0	39.727093	44
TCGGTGG	205	0.0	39.265682	45
GTGTCGA	90	1.03682396E-10	38.894924	36
TCCGACT	55	6.678374E-6	38.17928	20
TGTCGAT	75	3.5157427E-8	37.33912	37
CTCGGTT	75	3.522655E-8	37.330853	44
ACGCTTC	85	2.561137E-9	37.056362	15
>>END_MODULE