FastQCFastQC Report
Wed 25 May 2016
SRR1779719_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779719_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences225758
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT2470710.94401970251331TruSeq Adapter, Index 5 (95% over 23bp)
TCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG26811.187554815333233TruSeq Adapter, Index 5 (95% over 22bp)
CTCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC16190.7171395919524447RNA PCR Primer, Index 5 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTCTT10190.45136827930793144TruSeq Adapter, Index 5 (96% over 26bp)
TCTTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC7690.3406302323727177RNA PCR Primer, Index 5 (95% over 21bp)
CTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTC6620.29323434828444617TruSeq Adapter, Index 5 (95% over 24bp)
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGACCTCGTATGCCGT4750.21040228917690623No Hit
CTTTACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTC4260.18869763197760434RNA PCR Primer, Index 5 (95% over 24bp)
CTTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG2890.12801318225710717TruSeq Adapter, Index 5 (95% over 22bp)
TTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTC2370.1049796684945827TruSeq Adapter, Index 5 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAAGG252.3869096E-455.9999963
CGGTAAG252.3869096E-455.99999628
TAACGCT200.006944839452.512
AACGCTC200.006944839452.513
TTGGTAC200.006944839452.546
ACGCTCC200.006944839452.514
ATAACGC200.006944839452.511
CGCAAAG200.006944839452.512
ATCGCAG200.006944839452.517
GGCTAAA200.006944839452.51
TCTTTAT1450.050.6896551
AGCGTCA352.0820988E-550.03
TCTCGTA34600.049.8699438
ATCTCGT34800.049.68390737
TCGTATG36150.049.6680540
CGTATGC36200.049.5994541
CGTCTTC36200.049.5994548
TATGCCG36200.049.5994543
CTCGTAT35850.049.5955439
CCGTCTT36200.049.50276647
Produced by FastQC (version 0.11.5)