##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779719_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	225758
Sequences flagged as poor quality	0
Sequence length	76
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.69749909194802	34.0	34.0	34.0	34.0	34.0
2	33.703678274967	34.0	34.0	34.0	34.0	34.0
3	33.72099770550767	34.0	34.0	34.0	34.0	34.0
4	33.75286811541562	34.0	34.0	34.0	34.0	34.0
5	33.742184108647315	34.0	34.0	34.0	34.0	34.0
6	37.43567891281815	38.0	38.0	38.0	37.0	38.0
7	37.427435572604296	38.0	38.0	38.0	37.0	38.0
8	37.330499915839084	38.0	38.0	38.0	37.0	38.0
9	37.336103261014	38.0	38.0	38.0	37.0	38.0
10-11	37.26297185481799	38.0	38.0	38.0	36.0	38.0
12-13	37.230235473383004	38.0	38.0	38.0	36.0	38.0
14-15	37.246812959009205	38.0	38.0	38.0	36.0	38.0
16-17	37.2297260783671	38.0	38.0	38.0	36.0	38.0
18-19	37.21805207345919	38.0	38.0	38.0	36.0	38.0
20-21	37.24201578681597	38.0	38.0	38.0	36.0	38.0
22-23	37.19907157221449	38.0	38.0	38.0	36.0	38.0
24-25	37.177023627069694	38.0	38.0	38.0	36.0	38.0
26-27	37.108490064582426	38.0	38.0	38.0	36.0	38.0
28-29	37.09140761346221	38.0	38.0	38.0	36.0	38.0
30-31	36.97278280282426	38.0	38.0	38.0	35.5	38.0
32-33	36.926489869683465	38.0	38.0	38.0	35.0	38.0
34-35	36.89646878515933	38.0	37.5	38.0	35.0	38.0
36-37	36.99189618972528	38.0	38.0	38.0	36.0	38.0
38-39	36.41301304937145	38.0	37.0	38.0	34.0	38.0
40-41	36.856707182026774	38.0	37.0	38.0	35.0	38.0
42-43	37.038293216630194	38.0	38.0	38.0	36.0	38.0
44-45	37.05768566340949	38.0	38.0	38.0	36.0	38.0
46-47	37.071851274373444	38.0	38.0	38.0	36.0	38.0
48-49	37.04603823563285	38.0	38.0	38.0	36.0	38.0
50-51	36.99895684759787	38.0	38.0	38.0	36.0	38.0
52-53	36.99304565065246	38.0	38.0	38.0	36.0	38.0
54-55	36.99141558660158	38.0	38.0	38.0	36.0	38.0
56-57	37.00727548968365	38.0	38.0	38.0	36.0	38.0
58-59	36.95047794541057	38.0	38.0	38.0	36.0	38.0
60-61	37.004462743291484	38.0	38.0	38.0	36.0	38.0
62-63	37.01147467642343	38.0	38.0	38.0	36.0	38.0
64-65	37.02531028800752	38.0	38.0	38.0	36.0	38.0
66-67	36.97803843053181	38.0	38.0	38.0	36.0	38.0
68-69	36.83065051958292	38.0	38.0	38.0	35.0	38.0
70-71	35.83088971376429	38.0	37.5	38.0	29.5	38.0
72-73	34.19741714579328	38.0	37.0	38.0	21.5	38.0
74-75	33.997984567545785	38.0	37.0	38.0	20.0	38.0
76	33.61175240744514	38.0	35.0	38.0	19.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	1.0
21	5.0
22	15.0
23	24.0
24	58.0
25	86.0
26	178.0
27	308.0
28	605.0
29	964.0
30	1584.0
31	2687.0
32	4142.0
33	6861.0
34	11908.0
35	25886.0
36	34254.0
37	136191.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.63214592616873	16.178828657234735	11.009576626298957	36.17944879029758
2	18.87773633713977	16.626210366853	40.5956821020739	23.900371193933324
3	17.85761744877258	18.809964652415417	37.00245395512008	26.32996394369192
4	13.116257231194464	25.277066593431908	32.35632845790625	29.250347717467378
5	13.42986738011499	31.11340461910541	41.07318456045854	14.38354344032105
6	27.610095766262987	41.97857883220085	15.847943372992319	14.56338202854384
7	26.063306726671925	26.02122626883654	18.687709848598942	29.227757155892593
8	22.966627982175606	40.67054102180211	16.356895436706562	20.005935559315727
9	21.81982476811453	15.736319421681625	16.960196316409608	45.483659493794235
10-11	23.314123973458305	27.26813667732705	27.415418279750885	22.00232106946376
12-13	22.936285757315357	18.938199310766397	28.41936941326553	29.706145518652715
14-15	21.387060480691712	19.506949919825654	20.10006289921066	39.00592670027197
16-17	28.23532277925921	27.546089175134437	20.300941716351137	23.91764632925522
18-19	27.964678992549548	22.99121182859522	21.12571868992461	27.918390488930623
20-21	21.31109418049416	21.766227553397886	20.89294731526679	36.02973095084116
22-23	21.937871526147468	27.972873608022752	20.92461839669026	29.16463646913952
24-25	27.98616217365498	28.182832945011917	20.976443802655943	22.85456107867717
26-27	27.648411130502577	29.290434890457924	21.159161580099045	21.901992398940457
28-29	21.339886072697315	29.27138794638507	21.655489506462672	27.733236474454948
30-31	21.036463824094827	29.22199877745196	21.2508526829614	28.49068471549181
32-33	20.714880535794965	35.86982521106672	21.640650608173353	21.774643644964964
34-35	27.887383835788764	23.27625156140646	28.075195563390885	20.761169039413886
36-37	28.245067727389504	27.218526032300073	23.1823456976054	21.35406054270502
38-39	21.066141620673466	22.69487681499659	28.654577024955923	27.58440453937402
40-41	20.499384296459038	20.477679639259737	29.995614773341366	29.027321290939856
42-43	26.805030165043988	20.38953215389931	29.67823953082504	23.127198150231663
44-45	20.33814970012137	26.407037624358825	31.036773890626247	22.218038784893558
46-47	25.97272300427892	20.37114963810806	25.51249568121617	28.14363167639685
48-49	25.380938881457137	19.92996925911817	24.86024858476776	29.828843274656936
50-51	19.62831881926665	20.467048786753956	31.047626219225897	28.857006174753497
52-53	17.730711646984826	20.679887312963437	37.66798961720072	23.92141142285102
54-55	17.653637966317916	20.823182345697607	32.14216107513355	29.38101861285093
56-57	23.846995455310555	20.790846835992525	25.52711310341162	29.835044605285304
58-59	18.20068391817787	20.862605090406543	36.14268375871508	24.794027232700504
60-61	23.970800591784123	27.28319705171024	24.906537088386678	23.83946526811896
62-63	17.63636283099602	35.88377820498055	22.407843797340515	24.07201516668291
64-65	17.56704081361458	37.006883476997494	20.926168729347356	24.499906980040574
66-67	17.56283276783104	36.98074929792078	20.274364585086683	25.182053349161492
68-69	17.56947705064715	37.146413416135864	20.384438203740288	24.899671329476696
70-71	17.978986348213574	36.00005315426253	20.406807289221202	25.6141532083027
72-73	18.389381550155477	33.228722791661866	20.862605090406543	27.519290567776117
74-75	18.860018249630134	32.67835469839386	20.979765944063995	27.481861107912014
76	18.985816670948537	32.96007228979704	20.684095358746976	27.370015680507446
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	1.5
16	9.0
17	15.0
18	16.5
19	36.5
20	73.5
21	92.0
22	126.5
23	176.0
24	254.5
25	318.0
26	430.5
27	633.5
28	862.5
29	1001.0
30	1231.0
31	1522.5
32	2099.5
33	2615.0
34	2921.5
35	3600.0
36	4469.0
37	4966.0
38	5662.5
39	12134.0
40	18877.5
41	18517.0
42	17188.0
43	15499.5
44	13287.0
45	12241.0
46	11719.0
47	11427.0
48	10807.0
49	10096.0
50	9713.0
51	9203.5
52	7864.5
53	6582.0
54	6129.0
55	5780.5
56	4944.0
57	4206.5
58	3957.0
59	3641.5
60	3255.0
61	3025.0
62	2866.0
63	2810.0
64	2706.0
65	2612.5
66	2483.5
67	2400.0
68	2352.5
69	2304.5
70	2240.0
71	2176.0
72	2090.5
73	1934.5
74	1748.5
75	1633.0
76	1574.5
77	1370.5
78	1161.5
79	1098.0
80	964.0
81	711.5
82	472.0
83	351.0
84	301.0
85	187.0
86	104.0
87	85.0
88	63.0
89	30.5
90	13.0
91	3.5
92	1.0
93	1.5
94	1.0
95	0.5
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	225758.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.43365905084205
#Duplication Level	Percentage of deduplicated	Percentage of total
1	43.52629502282848	13.681907174939537
2	24.061495969787497	15.126817211350208
3	13.812637393608027	13.02545203270759
4	7.035961896172707	8.846641093560361
5	3.5990079476917876	5.656499437450722
6	1.9249196775829998	3.63043613072405
7	1.1879262724761852	2.6138608598587867
8	0.8004058395806325	2.0127747410944465
9	0.6228510230539428	1.7620638028331221
>10	3.3566315314807507	17.581215283622285
>50	0.045093286736937035	0.87527352297593
>100	0.018319147736880672	1.2531117391188795
>500	0.0028183304210585647	0.6338645806571639
>1k	0.0042274956315878475	2.356062686593609
>5k	0.0	0.0
>10k+	0.0014091652105292824	10.94401970251331
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT	24707	10.94401970251331	TruSeq Adapter, Index 5 (95% over 23bp)
TCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG	2681	1.187554815333233	TruSeq Adapter, Index 5 (95% over 22bp)
CTCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC	1619	0.7171395919524447	RNA PCR Primer, Index 5 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTCTT	1019	0.45136827930793144	TruSeq Adapter, Index 5 (96% over 26bp)
TCTTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC	769	0.3406302323727177	RNA PCR Primer, Index 5 (95% over 21bp)
CTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTC	662	0.29323434828444617	TruSeq Adapter, Index 5 (95% over 24bp)
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGACCTCGTATGCCGT	475	0.21040228917690623	No Hit
CTTTACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTC	426	0.18869763197760434	RNA PCR Primer, Index 5 (95% over 24bp)
CTTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG	289	0.12801318225710717	TruSeq Adapter, Index 5 (95% over 22bp)
TTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTC	237	0.1049796684945827	TruSeq Adapter, Index 5 (95% over 24bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	4.4295218774085523E-4	0.0
2	0.0	0.0	0.0	4.4295218774085523E-4	0.0
3	0.0	0.0	0.0	4.4295218774085523E-4	0.0
4	0.0	0.0	0.0	0.003543617501926842	0.0
5	0.0	0.0	0.0	0.0048724740651494075	0.0
6	0.0	0.0	0.0	0.0048724740651494075	0.0
7	0.0	0.0	0.0	0.006201330628371974	0.0
8	0.0	0.0	0.0	0.007973139379335395	0.0
9	0.0	0.0	0.0	0.009301995942557961	0.0
10	0.0	0.0	0.0	0.011959709069003092	0.0
11	0.0	0.0	0.0	0.012845613444484802	0.0
12	0.0	0.0	0.0	0.013731517819966513	0.0
13	0.0	0.0	0.0	0.014617422195448223	0.0
14	0.0	0.0	0.0	0.0168321831341525	0.0
15	0.0	0.0	0.0	0.01948989626059763	0.0
16	0.0	0.0	0.0	0.01948989626059763	0.0
17	0.0	0.0	0.0	0.021704657199301907	0.0
18	0.0	0.0	0.0	0.023033513762524473	0.0
19	0.0	0.0	0.0	0.02524827470122875	0.0
20	0.0	0.0	0.0	0.027463035639933027	0.0
21	0.0	0.0	0.0	0.030563700954119012	0.0
22	0.0	0.0	0.0	0.03277846189282329	0.0
23	0.0	0.0	0.0	0.04119455345989954	0.0
24	0.0	0.0	0.0	0.043409314398603814	0.0
25	0.0	0.0	0.0	0.046067027525048945	0.0
26	0.0	0.0	0.0	0.05581197565534776	0.0
27	0.0	0.0	0.0	0.11339576006165894	0.0
28	0.0	0.0	0.0	0.16522116602733902	0.0
29	0.0	0.0	0.0	0.2148318110543148	0.0
30	0.0	0.0	0.0	0.28393235234188824	0.0
31	0.0	0.0	0.0	0.3472745151888305	0.0
32	0.0	0.0	0.0	0.4190327696028491	0.0
33	0.0	0.0	0.0	0.5240124380974318	0.0
34	0.0	0.0	0.0	0.6644282816112829	0.0
35	0.0	0.0	0.0	0.865971527033372	0.0
36	0.0	0.0	0.0	1.2008433809654586	0.0
37	0.0	0.0	0.0	1.735486671568671	0.0
38	0.0	0.0	0.0	2.3139822287582277	0.0
39	0.0	0.0	0.0	3.005430593821703	0.0
40	0.0	0.0	0.0	3.8749457383570016	0.0
41	0.0	0.0	0.0	4.88399082203067	0.0
42	0.0	0.0	0.0	5.952391498861613	0.0
43	0.0	0.0	0.0	7.067745107593087	0.0
44	0.0	0.0	0.0	8.103367322531206	0.0
45	0.0	0.0	0.0	9.146962676848661	0.0
46	0.0	0.0	0.0	10.191000983353856	0.0
47	0.0	0.0	0.0	11.146448852310881	0.0
48	0.0	0.0	0.0	12.02127942309907	0.0
49	0.0	0.0	0.0	12.834982591979022	0.0
50	0.0	0.0	0.0	13.612363681464222	0.0
51	0.0	0.0	0.0	14.518643857582013	0.0
52	0.0	0.0	0.0	15.45460183027844	0.0
53	0.0	0.0	0.0	16.487566332090115	0.0
54	0.0	0.0	0.0	17.48022218481737	0.0
55	0.0	0.0	0.0	18.28816697525669	0.0
56	0.0	0.0	0.0	19.13154794071528	0.0
57	0.0	0.0	0.0	19.8571036242348	0.0
58	0.0	0.0	0.0	20.543679515233126	0.0
59	0.0	0.0	0.0	21.23468492810886	0.0
60	0.0	0.0	0.0	21.885381691900175	0.0
61	0.0	0.0	0.0	22.578601865714614	0.0
62	0.0	0.0	0.0	23.282895844222576	0.0
63	0.0	0.0	0.0	23.96902878303316	0.0
64	0.0	0.0	0.0	24.717617980315204	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTAAGG	25	2.3869096E-4	55.999996	3
CGGTAAG	25	2.3869096E-4	55.999996	28
TAACGCT	20	0.0069448394	52.5	12
AACGCTC	20	0.0069448394	52.5	13
TTGGTAC	20	0.0069448394	52.5	46
ACGCTCC	20	0.0069448394	52.5	14
ATAACGC	20	0.0069448394	52.5	11
CGCAAAG	20	0.0069448394	52.5	12
ATCGCAG	20	0.0069448394	52.5	17
GGCTAAA	20	0.0069448394	52.5	1
TCTTTAT	145	0.0	50.689655	1
AGCGTCA	35	2.0820988E-5	50.0	3
TCTCGTA	3460	0.0	49.86994	38
ATCTCGT	3480	0.0	49.683907	37
TCGTATG	3615	0.0	49.66805	40
CGTATGC	3620	0.0	49.59945	41
CGTCTTC	3620	0.0	49.59945	48
TATGCCG	3620	0.0	49.59945	43
CTCGTAT	3585	0.0	49.59554	39
CCGTCTT	3620	0.0	49.502766	47
>>END_MODULE