FastQCFastQC Report
Wed 25 May 2016
SRR1779718_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779718_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences171951
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACCC200.006942834252.49847846
GCGCCAA200.006942834252.49847855
AAGGATC200.006942834252.49847815
TACCGAC200.006942834252.49847819
TAGATCT1500.051.3318439
ACGTATG352.0803645E-549.99854770
GTAGATC1400.047.4986238
CACGTGT305.8662286E-446.6653133
ACGCTTC703.8016879E-1044.9986915
GACGCTT404.579228E-543.7487314
ATCTCGG1050.043.33207342
GCCGACT953.6379788E-1240.52513520
TCTCGTT350.001252972639.99883743
CGGTGTT350.001252972639.99883746
TGTCGAC350.001252972639.99883719
TAGATCC459.1708614E-538.8877639
GATCTCG1350.038.8877641
GTGGTCG1450.038.61956848
GTAGCTC556.667382E-638.18070638
GTGTATA654.8391485E-737.6912136