##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779718_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	171951
Sequences flagged as poor quality	0
Sequence length	76
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.50797610947305	34.0	27.0	34.0	12.0	34.0
2	28.14069124343563	34.0	23.0	34.0	12.0	34.0
3	27.002553052904606	32.0	23.0	34.0	11.0	34.0
4	29.335194328616872	34.0	24.0	34.0	12.0	34.0
5	28.918540747073294	34.0	23.0	34.0	11.0	34.0
6	31.339701426569196	37.0	27.0	38.0	11.0	38.0
7	32.339271071409875	37.0	31.0	38.0	12.0	38.0
8	31.5370483451681	37.0	27.0	38.0	11.0	38.0
9	32.9477990822967	37.0	33.0	38.0	12.0	38.0
10-11	32.648120685544136	37.0	31.5	38.0	11.0	38.0
12-13	31.6840698803729	37.0	29.5	38.0	11.0	38.0
14-15	31.68861477979192	37.0	29.0	38.0	11.5	38.0
16-17	31.39568830655245	36.5	28.0	38.0	11.0	38.0
18-19	31.96257654797006	37.0	29.0	38.0	11.5	38.0
20-21	30.865868764938853	36.0	27.0	38.0	11.0	38.0
22-23	30.844746468470667	35.5	26.5	38.0	11.0	38.0
24-25	32.02522520950736	37.0	29.0	38.0	11.0	38.0
26-27	30.605867369192385	36.5	25.0	38.0	11.0	38.0
28-29	32.656849916545994	37.0	32.0	38.0	11.0	38.0
30-31	33.11649248913935	38.0	34.0	38.0	11.0	38.0
32-33	33.186268181051574	38.0	34.0	38.0	11.0	38.0
34-35	33.25354316055155	38.0	34.0	38.0	11.0	38.0
36-37	31.211746369605294	36.5	27.5	38.0	11.0	38.0
38-39	29.570005408517545	34.5	23.0	38.0	11.0	38.0
40-41	30.710685020732647	35.0	26.0	38.0	11.0	38.0
42-43	31.73542171897808	36.5	29.0	38.0	11.0	38.0
44-45	33.26370884728789	37.5	34.0	38.0	11.0	38.0
46-47	31.86484230972777	37.0	29.0	38.0	11.0	38.0
48-49	31.322708213386374	36.5	27.5	38.0	11.0	38.0
50-51	29.235613634116696	34.5	22.5	38.0	11.0	38.0
52-53	32.48226238870376	37.0	32.5	38.0	11.0	38.0
54-55	31.588903234060865	36.5	28.5	38.0	11.0	38.0
56-57	31.97297195131171	37.0	29.5	38.0	11.0	38.0
58-59	33.017275270280486	37.5	34.0	38.0	11.0	38.0
60-61	33.44817709696367	38.0	34.0	38.0	11.0	38.0
62-63	33.35382463608819	38.0	34.0	38.0	11.0	38.0
64-65	33.07344534198697	38.0	33.0	38.0	11.0	38.0
66-67	31.107542264947572	37.0	26.5	38.0	11.0	38.0
68-69	31.211379986158846	37.0	26.0	38.0	11.0	38.0
70-71	30.777009147954942	36.5	25.5	38.0	11.0	38.0
72-73	29.609586451954335	34.5	23.5	38.0	11.0	38.0
74-75	31.6935086158266	36.5	28.5	38.0	11.0	38.0
76	32.18476193799396	37.0	31.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	2.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	4.0
12	44.0
13	330.0
14	810.0
15	1453.0
16	2260.0
17	3528.0
18	4399.0
19	4593.0
20	3988.0
21	3322.0
22	2643.0
23	2158.0
24	2102.0
25	2212.0
26	2470.0
27	2861.0
28	3411.0
29	4129.0
30	5223.0
31	7037.0
32	9096.0
33	12662.0
34	17978.0
35	25083.0
36	30252.0
37	17901.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.77297020662863	19.481712813534088	12.85831428720973	29.88700269262755
2	20.407557967095276	17.00717064745189	39.777611063616966	22.807660321835872
3	19.928933242609816	19.36074812010398	35.853818820477926	24.856499816808277
4	14.109252054364324	19.346790655477434	34.221958581223724	32.32199870893452
5	14.421550325383395	30.507528307482946	38.76220551203541	16.308715855098256
6	26.76692778756739	34.30163244180028	16.990886938720916	21.94055283191142
7	25.952160789992497	26.290629307186347	21.67826880913749	26.078941093683667
8	22.441537897865064	33.23950706314081	18.281001924989386	26.037953114004736
9	23.076028357245463	16.07278902465266	20.319397030514864	40.53178558758702
10-11	24.995856317443884	24.37561246536027	26.798140140797972	23.830391076397873
12-13	24.372123048470907	20.131609173677	28.516677958807435	26.97958981904466
14-15	23.521509284729774	22.9989706250109	23.372627930374705	30.10689215988462
16-17	24.836213589692143	23.12841494302007	25.85717068972035	26.17820077756744
18-19	22.286518520241703	22.848901114878423	27.715807777978096	27.148772586901774
20-21	24.222880040011862	22.813450499857517	27.05133498886298	25.912334471267645
22-23	24.94983977807373	21.596810682237173	27.521823331336613	25.93152620835248
24-25	22.85299710960808	24.638119442392803	24.14960249841523	28.359280949583887
26-27	23.380769879440997	23.884989153760706	27.08651984018517	25.64772112661312
28-29	23.408685133382573	24.654403340525388	26.068485423003334	25.868426103088705
30-31	23.228601753442184	24.376335800171564	26.186190552348826	26.208871894037422
32-33	22.956225392412865	24.56629582027229	25.866972183612585	26.610506603702262
34-35	23.260094562922724	24.235092963611304	26.201955230911487	26.302857242554477
36-37	24.127212138482925	21.9684325003344	29.895492268056227	24.00886309312645
38-39	23.865424039314327	21.13115922011079	32.822809287718634	22.18060745285625
40-41	23.572533556656662	23.317223811850095	25.48822899946496	27.622013632028285
42-43	21.226061215825624	23.73378152824384	27.006263485103144	28.033893770827394
44-45	21.0609572052097	22.916454636126517	28.858059244483087	27.164528914180696
46-47	23.45201193377106	22.024263008217552	28.961785180489564	25.561939877521823
48-49	23.382068997603927	22.386709935561914	29.718868495126433	24.512352571707726
50-51	24.8695834229917	21.11585411953544	31.018499671414197	22.99606278605866
52-53	20.552605714485107	23.65672379600928	28.95742342206119	26.833247067444415
54-55	22.409551669390343	23.07021267934097	28.365968979174056	26.154266672094632
56-57	22.390069148410284	23.152795305584796	27.61516496170376	26.841970584301162
58-59	20.627517120051177	24.356271536370112	28.00971226682563	27.006499076753077
60-61	20.636822285872142	23.49874234868201	27.952427339740325	27.91200802570552
62-63	20.513879276753883	24.267361454401648	27.652997726043488	27.56576154280098
64-65	20.687238657974167	24.926577066455753	26.987653315808757	27.398530959761324
66-67	20.037453183520597	25.0116314234536	23.684195221811244	31.26672017121455
68-69	20.002501221526792	25.657879429488823	24.15569231485144	30.18392703413295
70-71	20.4556028566776	25.57081210598553	23.40271477423407	30.5708702631028
72-73	20.002151800824663	25.20602038976673	22.673874230149636	32.117953579258966
74-75	21.182505226855717	26.040355106325443	25.3349113251933	27.442228341625547
76	21.688989177023423	25.40288108683389	25.790786803063696	27.11734293307899
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.5
2	0.5
3	0.5
4	1.0
5	0.5
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.5
14	1.0
15	1.5
16	5.0
17	8.0
18	6.5
19	7.0
20	17.0
21	25.0
22	35.5
23	75.0
24	133.5
25	163.0
26	234.5
27	386.0
28	567.0
29	668.0
30	802.0
31	1113.5
32	1589.5
33	1888.0
34	2212.5
35	2952.5
36	3894.5
37	4421.0
38	5011.0
39	6239.0
40	7586.0
41	8788.5
42	9282.0
43	9608.5
44	10225.0
45	10622.5
46	10730.0
47	10706.5
48	10309.0
49	9487.5
50	9040.0
51	8465.5
52	7393.0
53	6473.5
54	6052.0
55	5625.0
56	4805.5
57	4111.0
58	3809.0
59	3576.0
60	3177.5
61	2842.5
62	2673.0
63	2523.0
64	2319.0
65	2182.0
66	2104.0
67	2109.0
68	2039.0
69	1898.0
70	1725.0
71	1623.0
72	1578.5
73	1468.5
74	1266.5
75	1130.0
76	1044.5
77	848.5
78	634.5
79	531.0
80	464.0
81	316.0
82	188.0
83	141.0
84	112.0
85	62.5
86	40.0
87	38.0
88	25.5
89	10.5
90	5.0
91	2.0
92	2.0
93	1.0
94	1.0
95	1.0
96	0.0
97	0.5
98	0.5
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.001163122052212549
9	0.001163122052212549
10-11	0.001453902565265686
12-13	0.001453902565265686
14-15	0.001163122052212549
16-17	0.002617024617478235
18-19	0.002326244104425098
20-21	0.001163122052212549
22-23	0.001163122052212549
24-25	0.001163122052212549
26-27	0.001163122052212549
28-29	0.001163122052212549
30-31	0.0020354635913719605
32-33	0.001163122052212549
34-35	0.001163122052212549
36-37	0.001163122052212549
38-39	0.0020354635913719605
40-41	0.0017446830783188233
42-43	0.001163122052212549
44-45	0.001453902565265686
46-47	0.001163122052212549
48-49	0.0017446830783188233
50-51	0.001163122052212549
52-53	0.001163122052212549
54-55	0.001163122052212549
56-57	0.001163122052212549
58-59	0.0020354635913719605
60-61	0.0020354635913719605
62-63	0.002326244104425098
64-65	0.001163122052212549
66-67	0.0017446830783188233
68-69	0.020354635913719606
70-71	0.0017446830783188233
72-73	0.001163122052212549
74-75	0.001453902565265686
76	0.001163122052212549
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	171951.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	88.2446841570877
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.71726550772357	81.81805810637908
2	4.770918537092221	8.420163988897961
3	1.2301033992236385	3.2565025783514985
4	0.5266893675995918	1.8591014757088895
5	0.2661863415312136	1.1744764817676312
6	0.17816559707642785	0.9433300105000996
7	0.10429410723093281	0.6442380386427372
8	0.06351879382492784	0.44841567192959436
9	0.0575460447464163	0.45703192888234295
>10	0.08531230395104748	0.9786817189401781
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.002326244104425098	0.0
2	0.0	0.0	0.0	0.0029078051305313725	0.0
3	0.0	0.0	0.0	0.0029078051305313725	0.0
4	0.0	0.0	0.0	0.00523404923495647	0.0
5	0.0	0.0	0.0	0.006397171287169019	0.0
6	0.0	0.0	0.0	0.007560293339381568	0.0
7	0.0	0.0	0.0	0.007560293339381568	0.0
8	0.0	0.0	0.0	0.007560293339381568	0.0
9	0.0	0.0	0.0	0.01046809846991294	0.0
10	0.0	0.0	0.0	0.012794342574338037	0.0
11	0.0	0.0	0.0	0.012794342574338037	0.0
12	0.0	0.0	0.0	0.012794342574338037	0.0
13	0.0	0.0	0.0	0.013957464626550587	0.0
14	0.0	0.0	0.0	0.014539025652656861	0.0
15	0.0	0.0	0.0	0.01570214770486941	0.0
16	0.0	0.0	0.0	0.01570214770486941	0.0
17	0.0	0.0	0.0	0.01570214770486941	0.0
18	0.0	0.0	0.0	0.01570214770486941	0.0
19	0.0	0.0	0.0	0.01570214770486941	0.0
20	0.0	0.0	0.0	0.01570214770486941	0.0
21	0.0	0.0	0.0	0.016283708730975684	0.0
22	0.0	0.0	0.0	0.020354635913719606	0.0
23	0.0	0.0	0.0	0.02093619693982588	0.0
24	0.0	0.0	0.0	0.027914929253101173	0.0
25	0.0	0.0	0.0	0.030241173357526272	0.0
26	0.0	0.0	0.0	0.033148978488057645	0.0
27	0.0	0.0	0.0	0.061645468767265094	0.0
28	0.0	0.0	0.0	0.09188664212479136	0.0
29	0.0	0.0	0.0	0.12038313240399881	0.0
30	0.0	0.0	0.0	0.16981581962303213	0.0
31	0.0	0.0	0.0	0.18784421143232666	0.0
32	0.0	0.0	0.0	0.22215631197259683	0.0
33	0.0	0.0	0.0	0.25472372943454824	0.0
34	0.0	0.0	0.0	0.31055358794075055	0.0
35	0.0	0.0	0.0	0.44547574599740625	0.0
36	0.0	0.0	0.0	0.5513198527487482	0.0
37	0.0	0.0	0.0	0.8269797791231223	0.0
38	0.0	0.0	0.0	1.108455315758559	0.0
39	0.0	0.0	0.0	1.4533210042395799	0.0
40	0.0	0.0	0.0	1.8400590866002524	0.0
41	0.0	0.0	0.0	2.2814639054149146	0.0
42	0.0	0.0	0.0	2.7472942873260404	0.0
43	0.0	0.0	0.0	3.2113799861588475	0.0
44	0.0	0.0	0.0	3.6923309547487366	0.0
45	0.0	0.0	0.0	4.051154107856308	0.0
46	0.0	0.0	0.0	4.475112095887782	0.0
47	0.0	0.0	0.0	5.023524143505999	0.0
48	0.0	0.0	0.0	5.3887444679007395	0.0
49	0.0	0.0	0.0	5.7051136661025525	0.0
50	0.0	0.0	0.0	6.165709998778722	0.0
51	0.0	0.0	0.0	6.704235508953132	0.0
52	0.0	0.0	0.0	7.157853109316026	0.0
53	0.0	0.0	0.0	7.706265156934243	0.0
54	0.0	0.0	0.0	8.20001046809847	0.0
55	0.0	0.0	0.0	8.556507377101616	0.0
56	0.0	0.0	0.0	8.926961750731312	0.0
57	0.0	0.0	0.0	9.275316805368972	0.0
58	0.0	0.0	0.0	9.576565416892022	0.0
59	0.0	0.0	0.0	9.93422544794738	0.0
60	0.0	0.0	0.0	10.16219737018104	0.0
61	0.0	0.0	0.0	10.404708318067357	0.0
62	0.0	0.0	0.0	10.570453210507644	0.0
63	0.0	0.0	0.0	10.817616646602811	0.0
64	0.0	0.0	0.0	11.069432570906828	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTACCC	20	0.0069428342	52.498478	46
GCGCCAA	20	0.0069428342	52.498478	55
AAGGATC	20	0.0069428342	52.498478	15
TACCGAC	20	0.0069428342	52.498478	19
TAGATCT	150	0.0	51.33184	39
ACGTATG	35	2.0803645E-5	49.998547	70
GTAGATC	140	0.0	47.49862	38
CACGTGT	30	5.8662286E-4	46.66531	33
ACGCTTC	70	3.8016879E-10	44.99869	15
GACGCTT	40	4.579228E-5	43.74873	14
ATCTCGG	105	0.0	43.332073	42
GCCGACT	95	3.6379788E-12	40.525135	20
TCTCGTT	35	0.0012529726	39.998837	43
CGGTGTT	35	0.0012529726	39.998837	46
TGTCGAC	35	0.0012529726	39.998837	19
TAGATCC	45	9.1708614E-5	38.88776	39
GATCTCG	135	0.0	38.88776	41
GTGGTCG	145	0.0	38.619568	48
GTAGCTC	55	6.667382E-6	38.180706	38
GTGTATA	65	4.8391485E-7	37.69121	36
>>END_MODULE