FastQCFastQC Report
Wed 25 May 2016
SRR1779718_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779718_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences171951
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT1773110.311658553890354No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG20961.2189519107187514No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC13660.7944123616611709No Hit
ATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTCTT9490.5519014137748545RNA PCR Primer, Index 48 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC6110.3553337869509337No Hit
CTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTC5170.3006670504969439No Hit
CTTTACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTC3500.20354635913719604RNA PCR Primer, Index 48 (95% over 21bp)
CTTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG1980.11514908316904233No Hit
TTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTC1740.10119161854249174No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGACA200.00694203852.523
GGTTACA200.00694203852.51
AGTCGCG200.00694203852.512
CTTTATA1100.050.9090962
TATGCCG26950.047.53246743
ATCTCGT26850.047.44878837
CCGTCTT26850.047.44878847
CTCGTAT27250.047.39449739
TCTCGTA26900.047.36059638
GCCGTCT26950.047.27272846
ATGCCGT27050.047.22735644
TCGTATG27450.047.17668540
AATCTCG26450.047.1077536
CGTATGC27350.047.09323541
CGTCTTC27150.047.0534148
TCTTCTG27350.046.96526350
TGCCGTC27150.046.92449645
GTATGCC27300.046.9230842
GTCTTCT27450.046.7941749
CTTGAAA27200.046.70955757
Produced by FastQC (version 0.11.5)