##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779718_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	171951
Sequences flagged as poor quality	0
Sequence length	76
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.55692610104041	34.0	34.0	34.0	34.0	34.0
2	33.605649283807594	34.0	34.0	34.0	34.0	34.0
3	33.61961256404441	34.0	34.0	34.0	34.0	34.0
4	33.6529360108403	34.0	34.0	34.0	34.0	34.0
5	33.65131345557746	34.0	34.0	34.0	34.0	34.0
6	37.25631720664608	38.0	38.0	38.0	36.0	38.0
7	37.22550610348297	38.0	38.0	38.0	36.0	38.0
8	37.07779541846224	38.0	38.0	38.0	36.0	38.0
9	37.11026397054975	38.0	38.0	38.0	36.0	38.0
10-11	37.01893562701002	38.0	37.5	38.0	35.0	38.0
12-13	36.98293118388378	38.0	37.0	38.0	35.0	38.0
14-15	37.01376845729307	38.0	37.5	38.0	35.0	38.0
16-17	36.95773505242772	38.0	37.0	38.0	35.0	38.0
18-19	36.957421009473634	38.0	37.0	38.0	35.0	38.0
20-21	36.998328012049946	38.0	37.5	38.0	35.0	38.0
22-23	36.919218265668704	38.0	37.0	38.0	35.0	38.0
24-25	36.90888683403993	38.0	37.0	38.0	35.0	38.0
26-27	36.80723869009194	38.0	37.0	38.0	34.5	38.0
28-29	36.75758500968299	38.0	37.0	38.0	34.5	38.0
30-31	36.70869317421824	38.0	37.0	38.0	34.0	38.0
32-33	36.61533227489227	38.0	37.0	38.0	34.0	38.0
34-35	36.534972172304904	38.0	37.0	38.0	34.0	38.0
36-37	36.56135178044908	38.0	37.0	38.0	34.0	38.0
38-39	36.35881152188705	38.0	37.0	38.0	34.0	38.0
40-41	36.616853056975536	38.0	37.0	38.0	34.0	38.0
42-43	36.742886054748155	38.0	37.0	38.0	34.0	38.0
44-45	36.728030078336275	38.0	37.0	38.0	34.0	38.0
46-47	36.7506615256672	38.0	37.0	38.0	34.0	38.0
48-49	36.73354618466889	38.0	37.0	38.0	34.0	38.0
50-51	36.661793185267896	38.0	37.0	38.0	34.0	38.0
52-53	36.65364551529215	38.0	37.0	38.0	34.0	38.0
54-55	36.63056917377625	38.0	37.0	38.0	34.0	38.0
56-57	36.64576827119354	38.0	37.0	38.0	34.0	38.0
58-59	36.5789818029555	38.0	37.0	38.0	34.0	38.0
60-61	36.63854237544417	38.0	37.0	38.0	34.0	38.0
62-63	36.65673360434077	38.0	37.0	38.0	34.0	38.0
64-65	36.68831236805835	38.0	37.0	38.0	34.0	38.0
66-67	36.62903676047246	38.0	37.0	38.0	34.0	38.0
68-69	36.46464981302813	38.0	37.0	38.0	34.0	38.0
70-71	35.505216602404175	38.0	36.5	38.0	29.0	38.0
72-73	34.00490546725521	38.0	36.0	38.0	21.5	38.0
74-75	33.83454588807277	38.0	36.0	38.0	19.0	38.0
76	33.39827043750836	38.0	34.0	38.0	19.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	1.0
20	2.0
21	4.0
22	10.0
23	34.0
24	64.0
25	117.0
26	196.0
27	456.0
28	663.0
29	1141.0
30	1922.0
31	3017.0
32	4421.0
33	6964.0
34	11393.0
35	20947.0
36	28083.0
37	92515.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.90441462974917	17.468348541154167	11.369518060377667	35.257718768718995
2	18.225540997144535	17.13336939011695	41.33444993050346	23.306639682235055
3	17.482306005780718	20.21855063361074	37.00182028601171	25.297323074596832
4	13.105477723304896	25.695110816453525	33.795092788061716	27.404318672179866
5	13.021732935545591	31.589813377066722	41.61999639432164	13.768457293066048
6	26.20572139737483	42.85348733069305	16.545992753749612	14.394798518182505
7	24.751237271083042	26.828573256334654	20.43198353019174	27.988205942390564
8	21.941715953963627	41.61825171124332	16.930986153031967	19.509046181761082
9	21.726538374304308	15.624800088397276	18.083640106774602	44.565021430523814
10-11	23.338043977644794	27.510744339957316	27.652936010840296	21.498275671557597
12-13	22.664596309413728	19.321201970328755	28.257468697477773	29.75673302277975
14-15	21.648318416293012	19.723351419881247	20.308983373170264	38.31934679065548
16-17	28.187099813318913	27.442992480415935	20.07897598734523	24.290931718919925
18-19	28.213851620519797	22.88529871882106	21.049892120429657	27.85095754022948
20-21	21.55352396903769	21.906240731371145	20.942012550086943	35.598222749504224
22-23	22.205744659815878	27.69190059958942	20.962367186000662	29.139987554594043
24-25	27.92307110746666	28.28654674878308	20.863501811562596	22.926880332187658
26-27	27.611645177986755	29.06467540171328	21.733517106617583	21.590162313682388
28-29	21.46076498537374	22.47268117079866	28.624724485463883	27.441829358363716
30-31	21.259254089827916	20.792842146890685	35.87591813946997	22.071985623811436
32-33	26.30662223540427	21.88239672930079	23.916406418107485	27.894574617187455
34-35	22.60731254834226	21.61894958447465	28.34295816831539	27.430779698867703
36-37	21.266814383167297	33.15770190344924	22.584050107298008	22.991433606085454
38-39	20.76114707096789	23.56281731423487	28.532547062826037	27.143488551971203
40-41	20.738756971462802	20.608487301614993	29.75673302277975	28.89602270414246
42-43	26.75326110345389	20.778012340724974	29.49415821949276	22.97456833632837
44-45	20.689324284243767	27.12022611092695	30.18999598722892	22.000453617600364
46-47	26.481381323749208	21.259544870340967	24.55001715605027	27.709056649859555
48-49	25.961174985897145	20.666061843199515	23.670406104064533	29.702357066838807
50-51	20.502061633837545	21.104268076370595	29.81750615000785	28.576164139784012
52-53	18.988258282882914	20.830062052561484	36.56913888258864	23.612540781966956
54-55	18.74109484678775	21.014998458863282	31.601444597588845	28.642462096760124
56-57	24.927450261993243	21.14090642101529	25.0164290989875	28.915214218003964
58-59	19.577088821815515	21.44884298433856	35.01724328442405	23.95682490942187
60-61	25.074003640572023	27.79978016993213	24.584620037103594	22.541596152392252
62-63	18.57360527126914	35.78955632709319	22.381376089699973	23.255462311937702
64-65	18.5099243389105	37.09108990351903	21.360445708370406	23.038540049200062
66-67	18.505271850701654	36.89975632593006	21.13334612767591	23.46162569569238
68-69	18.40117242702863	37.23124611081064	21.17085681385976	23.19672464830097
70-71	18.71696006420434	35.58804543154736	21.739332716878646	23.95566178736966
72-73	19.072003070642218	31.45808980465365	23.10600112822839	26.36390599647574
74-75	19.203435862542236	29.897180010584414	24.012945548441124	26.886438578432227
76	19.27991113747521	29.50898802565847	24.220272054248014	26.990828782618305
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	2.5
15	2.5
16	5.5
17	11.0
18	14.0
19	18.0
20	24.5
21	30.0
22	42.0
23	72.5
24	145.5
25	200.0
26	263.5
27	380.5
28	517.5
29	601.0
30	784.0
31	1045.0
32	1471.5
33	1820.0
34	2217.5
35	2890.0
36	3437.0
37	3709.0
38	4458.0
39	9229.0
40	14358.0
41	14788.5
42	14112.0
43	12930.0
44	11167.0
45	9977.0
46	9368.0
47	9236.0
48	8599.5
49	7578.0
50	7061.0
51	6680.0
52	5562.5
53	4612.5
54	4399.0
55	4148.5
56	3565.5
57	2874.5
58	2516.0
59	2348.0
60	2129.5
61	2017.0
62	1955.0
63	1892.5
64	1812.5
65	1792.5
66	1804.5
67	1819.0
68	1813.5
69	1706.0
70	1648.5
71	1693.0
72	1678.5
73	1562.0
74	1392.0
75	1324.0
76	1274.0
77	1078.5
78	839.5
79	746.0
80	659.0
81	507.5
82	360.0
83	277.0
84	222.5
85	144.0
86	85.5
87	51.0
88	40.5
89	21.0
90	11.5
91	6.0
92	1.0
93	2.5
94	2.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	171951.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.253671103977297
#Duplication Level	Percentage of deduplicated	Percentage of total
1	49.723674661803834	15.540473739611866
2	21.49383152527865	13.435222825107152
3	10.848328092145662	10.171502346598741
4	5.612102491579986	7.0159522189460946
5	2.984685807856199	4.664119429372321
6	1.8496120280605124	3.468429959697821
7	1.3900001860776687	3.040982605509709
8	0.9917939748050837	2.479776215317154
9	0.749893005340429	2.1093218416874575
>10	4.25745706257792	22.301702229123414
>50	0.07257029083939637	1.3643421672453198
>100	0.014886213505517203	0.875249344289943
>500	0.005582330064568952	1.2079022512227322
>1k	0.003721553376379301	2.0133642723799223
>5k	0.0	0.0
>10k+	0.0018607766881896504	10.311658553890354
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT	17731	10.311658553890354	No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG	2096	1.2189519107187514	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC	1366	0.7944123616611709	No Hit
ATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTCTT	949	0.5519014137748545	RNA PCR Primer, Index 48 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC	611	0.3553337869509337	No Hit
CTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTC	517	0.3006670504969439	No Hit
CTTTACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTC	350	0.20354635913719604	RNA PCR Primer, Index 48 (95% over 21bp)
CTTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG	198	0.11514908316904233	No Hit
TTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTC	174	0.10119161854249174	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0029078051305313725	0.0
2	0.0	0.0	0.0	0.0034893661566376467	0.0
3	0.0	0.0	0.0	0.0034893661566376467	0.0
4	0.0	0.0	0.0	0.005815610261062745	0.0
5	0.0	0.0	0.0	0.006978732313275293	0.0
6	0.0	0.0	0.0	0.008141854365487842	0.0
7	0.0	0.0	0.0	0.008141854365487842	0.0
8	0.0	0.0	0.0	0.008141854365487842	0.0
9	0.0	0.0	0.0	0.012212781548231764	0.0
10	0.0	0.0	0.0	0.018028391809294508	0.0
11	0.0	0.0	0.0	0.018028391809294508	0.0
12	0.0	0.0	0.0	0.01977307488761333	0.0
13	0.0	0.0	0.0	0.022680880018144705	0.0
14	0.0	0.0	0.0	0.02326244104425098	0.0
15	0.0	0.0	0.0	0.026751807200888624	0.0
16	0.0	0.0	0.0	0.027914929253101173	0.0
17	0.0	0.0	0.0	0.027914929253101173	0.0
18	0.0	0.0	0.0	0.028496490279207448	0.0
19	0.0	0.0	0.0	0.029659612331419997	0.0
20	0.0	0.0	0.0	0.029659612331419997	0.0
21	0.0	0.0	0.0	0.03663834464469529	0.0
22	0.0	0.0	0.0	0.03780146669690784	0.0
23	0.0	0.0	0.0	0.040127710801332934	0.0
24	0.0	0.0	0.0	0.04536176003628941	0.0
25	0.0	0.0	0.0	0.0500142482451396	0.0
26	0.0	0.0	0.0	0.05292205337567098	0.0
27	0.0	0.0	0.0	0.08839727596815372	0.0
28	0.0	0.0	0.0	0.12329093753453019	0.0
29	0.0	0.0	0.0	0.1773761129624137	0.0
30	0.0	0.0	0.0	0.2413478258341039	0.0
31	0.0	0.0	0.0	0.2867095858703933	0.0
32	0.0	0.0	0.0	0.33148978488057645	0.0
33	0.0	0.0	0.0	0.40651115724828585	0.0
34	0.0	0.0	0.0	0.5519014137748545	0.0
35	0.0	0.0	0.0	0.750213723677094	0.0
36	0.0	0.0	0.0	1.0380864315996998	0.0
37	0.0	0.0	0.0	1.4399451006391355	0.0
38	0.0	0.0	0.0	1.9057754825502613	0.0
39	0.0	0.0	0.0	2.403010159871126	0.0
40	0.0	0.0	0.0	2.9333938156800485	0.0
41	0.0	0.0	0.0	3.498089572029241	0.0
42	0.0	0.0	0.0	4.138969822798355	0.0
43	0.0	0.0	0.0	4.754261388418794	0.0
44	0.0	0.0	0.0	5.384091979691889	0.0
45	0.0	0.0	0.0	6.088362382306587	0.0
46	0.0	0.0	0.0	6.755412879250484	0.0
47	0.0	0.0	0.0	7.482945722909434	0.0
48	0.0	0.0	0.0	8.137783438305098	0.0
49	0.0	0.0	0.0	8.74784095469058	0.0
50	0.0	0.0	0.0	9.30381329564818	0.0
51	0.0	0.0	0.0	9.90921832382481	0.0
52	0.0	0.0	0.0	10.529743938680205	0.0
53	0.0	0.0	0.0	11.117120575047542	0.0
54	0.0	0.0	0.0	11.650993597013102	0.0
55	0.0	0.0	0.0	12.234299306197697	0.0
56	0.0	0.0	0.0	12.784456036894232	0.0
57	0.0	0.0	0.0	13.346825549138998	0.0
58	0.0	0.0	0.0	13.87895388802624	0.0
59	0.0	0.0	0.0	14.36746514995551	0.0
60	0.0	0.0	0.0	14.865862949328587	0.0
61	0.0	0.0	0.0	15.379381335380428	0.0
62	0.0	0.0	0.0	15.853353571657042	0.0
63	0.0	0.0	0.0	16.36047478642171	0.0
64	0.0	0.0	0.0	16.875737855551872	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAGACA	20	0.006942038	52.5	23
GGTTACA	20	0.006942038	52.5	1
AGTCGCG	20	0.006942038	52.5	12
CTTTATA	110	0.0	50.909096	2
TATGCCG	2695	0.0	47.532467	43
ATCTCGT	2685	0.0	47.448788	37
CCGTCTT	2685	0.0	47.448788	47
CTCGTAT	2725	0.0	47.394497	39
TCTCGTA	2690	0.0	47.360596	38
GCCGTCT	2695	0.0	47.272728	46
ATGCCGT	2705	0.0	47.227356	44
TCGTATG	2745	0.0	47.176685	40
AATCTCG	2645	0.0	47.10775	36
CGTATGC	2735	0.0	47.093235	41
CGTCTTC	2715	0.0	47.05341	48
TCTTCTG	2735	0.0	46.965263	50
TGCCGTC	2715	0.0	46.924496	45
GTATGCC	2730	0.0	46.92308	42
GTCTTCT	2745	0.0	46.79417	49
CTTGAAA	2720	0.0	46.709557	57
>>END_MODULE