FastQCFastQC Report
Wed 25 May 2016
SRR1779717_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779717_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences150586
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCGA150.002221725469.9953468
GTAGATC1000.052.49651338
TCGACTA200.0069421952.49651321
CCTACGA1100.050.9057121
CGTGTAT352.0795766E-549.99667735
CGGAGTG352.0795766E-549.99667732
ATCGGTG451.6767426E-646.66356744
TCGGTGG1750.045.99694445
CGGTGGT1700.045.29110746
CCCGTGT703.8016879E-1044.9970133
GCCTACG1350.044.07114820
TGCCTAC1450.043.4453919
ATCTCGT757.4760464E-1041.99721542
AGATCTC1500.041.99721540
TCTCGGT1850.041.6188643
TTAGATC602.5811278E-740.8306238
CCGTGTA602.5811278E-740.8306234
GATCTCG1550.040.6424641
GTGTAGA2100.039.99734536
TGTAGAT2050.039.26568237
Produced by FastQC (version 0.11.5)