##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779717_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	150586
Sequences flagged as poor quality	0
Sequence length	76
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.007198544353393	34.0	23.0	34.0	12.0	34.0
2	27.579137502822306	34.0	23.0	34.0	12.0	34.0
3	26.63752274447824	32.0	21.0	34.0	11.0	34.0
4	28.795459073220616	33.0	23.0	34.0	12.0	34.0
5	28.403364190562204	34.0	23.0	34.0	11.0	34.0
6	30.728460813090194	36.0	26.0	38.0	11.0	38.0
7	31.667127090167746	37.0	27.0	38.0	12.0	38.0
8	30.904247406797445	37.0	26.0	38.0	11.0	38.0
9	32.361248721660715	37.0	31.0	38.0	12.0	38.0
10-11	31.99261219502477	37.0	31.0	38.0	11.0	38.0
12-13	31.116342156641387	36.0	27.0	38.0	11.0	38.0
14-15	31.04830794363354	36.5	26.5	38.0	11.0	38.0
16-17	30.76865711287902	35.5	27.0	38.0	11.0	38.0
18-19	31.34009137635637	37.0	27.0	38.0	11.5	38.0
20-21	30.247838444476912	35.5	24.5	38.0	11.0	38.0
22-23	30.24105826570863	35.5	26.0	38.0	11.0	38.0
24-25	31.351533343073058	36.5	28.5	38.0	11.0	38.0
26-27	29.990610016867436	35.0	24.0	38.0	11.0	38.0
28-29	31.951223885354548	37.0	29.5	38.0	11.0	38.0
30-31	32.43223473629687	37.0	31.0	38.0	11.0	38.0
32-33	32.49072622952997	38.0	31.0	38.0	11.0	38.0
34-35	32.551771080976984	38.0	31.5	38.0	11.0	38.0
36-37	30.63476020347177	36.0	25.0	38.0	11.0	38.0
38-39	29.004084044997544	34.0	22.5	37.5	11.0	38.0
40-41	30.057770974725408	35.0	24.5	37.5	11.0	38.0
42-43	31.048912249478704	36.5	27.0	38.0	11.0	38.0
44-45	32.61181318316444	37.0	32.0	38.0	11.0	38.0
46-47	31.23703398722325	36.5	28.5	38.0	11.0	38.0
48-49	30.704179007344642	36.0	25.0	38.0	11.0	38.0
50-51	28.612268072729204	34.0	21.5	37.5	11.0	38.0
52-53	31.76452990317825	36.5	29.0	38.0	11.0	38.0
54-55	30.870217018846375	36.5	27.0	38.0	11.0	38.0
56-57	31.297072769048917	36.5	27.0	38.0	11.0	38.0
58-59	32.32192899738355	37.0	31.0	38.0	11.0	38.0
60-61	32.78393077709747	38.0	34.0	38.0	11.0	38.0
62-63	32.67230353419309	38.0	33.0	38.0	11.0	38.0
64-65	32.38142323987621	37.5	31.0	38.0	11.0	38.0
66-67	30.456739006282124	36.5	24.5	38.0	11.0	38.0
68-69	30.536623590506423	36.5	25.0	38.0	11.0	38.0
70-71	30.133677765529335	35.5	24.0	38.0	11.0	38.0
72-73	28.97288592565046	34.0	22.5	37.5	11.0	38.0
74-75	30.93102612460654	36.0	26.0	38.0	11.0	38.0
76	31.359754558856732	37.0	27.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	5.0
4	1.0
5	2.0
6	0.0
7	0.0
8	0.0
9	1.0
10	0.0
11	9.0
12	61.0
13	295.0
14	820.0
15	1413.0
16	2306.0
17	3484.0
18	4483.0
19	4855.0
20	4379.0
21	3246.0
22	2716.0
23	2258.0
24	2074.0
25	2256.0
26	2447.0
27	2797.0
28	3425.0
29	4008.0
30	5039.0
31	6325.0
32	8058.0
33	11203.0
34	15093.0
35	20400.0
36	23776.0
37	13351.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.929249731050696	19.285989401405175	11.24008872006694	30.544672147477186
2	20.579602353472435	17.02083859057283	38.42189845005512	23.97766060589962
3	20.77298535710728	17.042866155327555	36.44918152538433	25.734966962180827
4	14.073395226521098	19.502330955891143	33.02585966450173	33.39841415308602
5	14.561114579044531	28.638120409599832	39.11054148460016	17.690223526755474
6	27.53708844115655	32.57075690967287	16.88005525082013	23.012099398350443
7	26.971962865073777	25.174983066154887	20.82796541511163	27.0250886536597
8	23.484018355813227	31.194920938510684	18.066264220110373	27.25479648556572
9	22.65189701087137	16.05868016124426	19.679370961422244	41.61005186646212
10-11	24.11373658484752	23.211866432897672	27.268953883753056	25.405443098501753
12-13	23.967630963765806	19.344384231218786	29.171979598342368	27.516005206673043
14-15	23.555897939938106	22.54280173730558	23.626957457264673	30.274342865491636
16-17	25.058608666777353	22.587082849078534	26.07205711439482	26.282251369749293
18-19	21.72597799774864	22.540851599363773	28.419630019491883	27.313540383395708
20-21	24.35639882187696	22.4557290715474	27.276138359725987	25.911733746849652
22-23	25.0683207539025	21.526599436167	27.494994238808847	25.910085571121655
24-25	22.09773769479301	24.981653843727276	24.18670908143037	28.733899380049344
26-27	23.501695129118698	23.759368037269596	27.139000587733307	25.599936245878396
28-29	22.972479379457823	24.606848277968894	26.19705401851532	26.223618324057963
30-31	22.432932316346285	24.35223525739579	26.675986465261616	26.53884596099631
32-33	22.85585259515801	24.196681465149407	26.343402278545742	26.604063661146842
34-35	23.01196737859951	24.15989267878015	26.27477154393795	26.55336839868239
36-37	24.271715705970053	21.55150372070011	30.326113304532914	23.850667268796926
38-39	23.9761848628438	20.656609763143653	33.27411647899905	22.093088895013498
40-41	23.352891757900572	23.313708496458563	25.85464338251165	27.478756363129214
42-43	20.487456625325827	23.720011289867344	27.50610150918962	28.286430575617207
44-45	20.72674264158963	22.07755552013601	29.894870364467113	27.300831473807246
46-47	23.259196291598318	21.222364637361615	29.62736672931457	25.891072341725497
48-49	23.08808537909554	21.703730022480418	30.62583222370173	24.582352374722316
50-51	25.443122879020315	20.322886989553655	31.38219804886472	22.851792082561314
52-53	19.417437041759644	23.514703538412498	29.734154181277226	27.333705238550632
54-55	21.697752954497908	22.541183658696134	28.805815023028313	26.95524836377764
56-57	21.978974352163	22.724767230272683	28.07813890475368	27.218119512810635
58-59	19.579214414031497	23.954096117894345	28.821421811649305	27.64526765642485
60-61	19.409660998376218	23.10318148159217	28.74756349846754	28.739594021564074
62-63	19.779245489774162	24.023988125479416	28.214604730548665	27.982161654197757
64-65	20.106522865225994	24.306671625337035	27.313086905125584	28.273718604311387
66-67	18.794683030659243	25.336959445324407	23.757342710751157	32.11101481326519
68-69	19.54442185818486	25.27601737813384	23.731847954614903	31.44771280906639
70-71	19.751352320612057	25.662711813753326	23.35820474250288	31.22773112313174
72-73	19.44818929849844	25.718071152964928	22.473551737649174	32.36018781088745
74-75	21.19665555393218	26.06954535191064	24.77951626399607	27.954282830161116
76	21.59241844649878	25.262990117947083	25.585086600786315	27.55950483476782
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.5
2	1.5
3	1.5
4	1.0
5	1.0
6	1.0
7	0.5
8	0.0
9	0.0
10	0.5
11	1.0
12	1.0
13	1.0
14	1.5
15	3.0
16	5.5
17	7.0
18	11.0
19	13.0
20	25.5
21	40.0
22	46.0
23	74.5
24	140.5
25	184.0
26	222.0
27	343.0
28	513.5
29	601.0
30	724.5
31	1041.0
32	1474.0
33	1714.0
34	2010.5
35	2649.5
36	3408.0
37	3824.0
38	4293.0
39	5253.5
40	6268.0
41	7175.5
42	7560.0
43	7884.5
44	8658.5
45	9067.5
46	9027.0
47	9036.5
48	8917.5
49	8371.5
50	7954.0
51	7595.0
52	6870.0
53	6064.5
54	5625.0
55	5209.5
56	4407.5
57	3746.5
58	3472.0
59	3277.0
60	2916.0
61	2579.0
62	2408.0
63	2358.5
64	2219.0
65	2016.5
66	1872.5
67	1841.0
68	1749.0
69	1580.5
70	1489.5
71	1475.0
72	1405.5
73	1236.0
74	1057.0
75	978.0
76	880.0
77	708.5
78	557.0
79	479.0
80	409.0
81	283.5
82	179.0
83	130.0
84	110.0
85	71.5
86	41.0
87	29.0
88	21.5
89	11.5
90	8.0
91	5.5
92	4.0
93	3.0
94	1.5
95	0.5
96	0.0
97	0.0
98	0.5
99	2.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	6.64072357324054E-4
4	0.002656289429296216
5	0.0
6	0.0
7	0.0
8	0.004648506501268378
9	0.004648506501268378
10-11	0.00664072357324054
12-13	0.00664072357324054
14-15	0.005312578858592432
16-17	0.007304795930564594
18-19	0.006972759751902567
20-21	0.004980542679930405
22-23	0.006308687394578513
24-25	0.006308687394578513
26-27	0.004980542679930405
28-29	0.005312578858592432
30-31	0.006972759751902567
32-33	0.004980542679930405
34-35	0.00664072357324054
36-37	0.006308687394578513
38-39	0.006308687394578513
40-41	0.007636832109226622
42-43	0.0056446150372544595
44-45	0.00664072357324054
46-47	0.0059766512159164864
48-49	0.006972759751902567
50-51	0.004648506501268378
52-53	0.00664072357324054
54-55	0.006972759751902567
56-57	0.005312578858592432
58-59	0.006972759751902567
60-61	0.007636832109226622
62-63	0.007636832109226622
64-65	0.005312578858592432
66-67	0.007636832109226622
68-69	0.034531762580850806
70-71	0.007636832109226622
72-73	0.0059766512159164864
74-75	0.005312578858592432
76	0.00664072357324054
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	150586.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	93.02207801551693
#Duplication Level	Percentage of deduplicated	Percentage of total
1	94.92384749227136	88.30013546959098
2	3.7351838188801336	6.949091212043284
3	0.8209845765498759	2.2910907398807607
4	0.2735484132716964	1.0178416736152245
5	0.11589044653251017	0.5390185079300127
6	0.051843865850833094	0.28935744802813146
7	0.034872438146892656	0.2270734663324076
8	0.019923094371294674	0.14826301111336634
9	0.009960919720251428	0.08339269061911765
>10	0.013944934405135095	0.15473578084670103
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0013281447146481081	0.0
2	0.0	0.0	0.0	0.0013281447146481081	0.0
3	0.0	0.0	0.0	0.00332036178662027	0.0
4	0.0	0.0	0.0	0.00796886828788865	0.0
5	0.0	0.0	0.0	0.008632940645212703	0.0
6	0.0	0.0	0.0	0.008632940645212703	0.0
7	0.0	0.0	0.0	0.011953302431832973	0.0
8	0.0	0.0	0.0	0.012617374789157027	0.0
9	0.0	0.0	0.0	0.014609591861129189	0.0
10	0.0	0.0	0.0	0.0159377365757773	0.0
11	0.0	0.0	0.0	0.01792995364774946	0.0
12	0.0	0.0	0.0	0.01792995364774946	0.0
13	0.0	0.0	0.0	0.018594026005073514	0.0
14	0.0	0.0	0.0	0.019258098362397568	0.0
15	0.0	0.0	0.0	0.020586243077045676	0.0
16	0.0	0.0	0.0	0.020586243077045676	0.0
17	0.0	0.0	0.0	0.020586243077045676	0.0
18	0.0	0.0	0.0	0.02125031543436973	0.0
19	0.0	0.0	0.0	0.021914387791693784	0.0
20	0.0	0.0	0.0	0.023906604863665946	0.0
21	0.0	0.0	0.0	0.025898821935638108	0.0
22	0.0	0.0	0.0	0.03253954550887865	0.0
23	0.0	0.0	0.0	0.03718805201014703	0.0
24	0.0	0.0	0.0	0.04117248615409135	0.0
25	0.0	0.0	0.0	0.043164703226063514	0.0
26	0.0	0.0	0.0	0.05378986094324838	0.0
27	0.0	0.0	0.0	0.08765755116677514	0.0
28	0.0	0.0	0.0	0.12086116903297783	0.0
29	0.0	0.0	0.0	0.1427755568246716	0.0
30	0.0	0.0	0.0	0.18992469419467944	0.0
31	0.0	0.0	0.0	0.20719057548510486	0.0
32	0.0	0.0	0.0	0.24371455513792784	0.0
33	0.0	0.0	0.0	0.2901996201506116	0.0
34	0.0	0.0	0.0	0.34465355345118404	0.0
35	0.0	0.0	0.0	0.45289734769500484	0.0
36	0.0	0.0	0.0	0.527937524072623	0.0
37	0.0	0.0	0.0	0.8705988604518349	0.0
38	0.0	0.0	0.0	1.1627906976744187	0.0
39	0.0	0.0	0.0	1.5565856055675826	0.0
40	0.0	0.0	0.0	2.0227644004090686	0.0
41	0.0	0.0	6.640723573240541E-4	2.6283983902886057	0.0
42	0.0	0.0	6.640723573240541E-4	3.3011036882578724	0.0
43	0.0	0.0	6.640723573240541E-4	4.010997038237286	0.0
44	0.0	0.0	6.640723573240541E-4	4.714913737000783	0.0
45	0.0	0.0	6.640723573240541E-4	5.157185926978603	0.0
46	0.0	0.0	6.640723573240541E-4	5.688443812837847	0.0
47	0.0	0.0	6.640723573240541E-4	6.256225678349913	0.0
48	0.0	0.0	6.640723573240541E-4	6.662637961032234	0.0
49	0.0	0.0	6.640723573240541E-4	7.021237033987223	0.0
50	0.0	0.0	6.640723573240541E-4	7.587690754784641	0.0
51	0.0	0.0	6.640723573240541E-4	8.247114605607427	0.0
52	0.0	0.0	6.640723573240541E-4	8.911851035288805	0.0
53	0.0	0.0	6.640723573240541E-4	9.691471982787245	0.0
54	0.0	0.0	6.640723573240541E-4	10.530859442444848	0.0
55	0.0	0.0	6.640723573240541E-4	11.029577782795213	0.0
56	0.0	0.0	6.640723573240541E-4	11.48579549227684	0.0
57	0.0	0.0	6.640723573240541E-4	11.884238906671271	0.0
58	0.0	0.0	6.640723573240541E-4	12.238853545482316	0.0
59	0.0	0.0	6.640723573240541E-4	12.608741848511814	0.0
60	0.0	0.0	6.640723573240541E-4	12.898941468662425	0.0
61	0.0	0.0	6.640723573240541E-4	13.221016561964591	0.0
62	0.0	0.0	6.640723573240541E-4	13.431527499236317	0.0
63	0.0	0.0	6.640723573240541E-4	13.732352277104113	0.0
64	0.0	0.0	6.640723573240541E-4	14.039153706187827	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAGCGA	15	0.0022217254	69.995346	8
GTAGATC	100	0.0	52.496513	38
TCGACTA	20	0.00694219	52.496513	21
CCTACGA	110	0.0	50.90571	21
CGTGTAT	35	2.0795766E-5	49.996677	35
CGGAGTG	35	2.0795766E-5	49.996677	32
ATCGGTG	45	1.6767426E-6	46.663567	44
TCGGTGG	175	0.0	45.996944	45
CGGTGGT	170	0.0	45.291107	46
CCCGTGT	70	3.8016879E-10	44.99701	33
GCCTACG	135	0.0	44.071148	20
TGCCTAC	145	0.0	43.44539	19
ATCTCGT	75	7.4760464E-10	41.997215	42
AGATCTC	150	0.0	41.997215	40
TCTCGGT	185	0.0	41.61886	43
TTAGATC	60	2.5811278E-7	40.83062	38
CCGTGTA	60	2.5811278E-7	40.83062	34
GATCTCG	155	0.0	40.64246	41
GTGTAGA	210	0.0	39.997345	36
TGTAGAT	205	0.0	39.265682	37
>>END_MODULE