FastQCFastQC Report
Wed 25 May 2016
SRR1779717_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779717_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences150586
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT1845812.25744757148739No Hit
TCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG22671.5054520340536304No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC15711.0432576733560888No Hit
ATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTCTT8820.5857118191598156TruSeq Adapter, Index 2 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC6520.43297517697528326No Hit
CTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTC5150.3419972640218878No Hit
CTTTACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTC2970.19722949012524407No Hit
CTTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG2130.1414474121100235No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTACCTCGTATGCCGT1800.11953302431832971No Hit
TTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTC1790.11886895196100568No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC1640.10890786660114485No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCA150.002221140570.02
TTGTACT150.002221140570.04
GAGTTGC150.002221140570.035
GTTATAG150.002221140570.016
TTAATAC252.3843459E-455.9999963
CATGCCG252.3843459E-455.99999643
CGCATGC252.3843459E-455.99999641
GCCTATG252.3843459E-455.9999961
GGCCGAA200.00694036752.512
CGATTTC200.00694036752.516
GGCCCTA200.00694036752.512
GCACAAA200.00694036752.523
TAGATTG200.00694036752.564
AATACGG200.00694036752.56
CTTGAAA26950.047.79220657
TCTCGTA27000.047.70370538
CTCGTAT27150.047.6979739
TCGTATG27250.047.65137540
TCTTCTG26750.047.62616750
CCGTCTT26750.047.62616747
Produced by FastQC (version 0.11.5)