##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779717_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	150586
Sequences flagged as poor quality	0
Sequence length	76
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.56853890799942	34.0	34.0	34.0	34.0	34.0
2	33.59741941481944	34.0	34.0	34.0	34.0	34.0
3	33.62623351440373	34.0	34.0	34.0	34.0	34.0
4	33.66401923153547	34.0	34.0	34.0	34.0	34.0
5	33.66886695974394	34.0	34.0	34.0	34.0	34.0
6	37.27054307837382	38.0	38.0	38.0	36.0	38.0
7	37.256165911837755	38.0	38.0	38.0	36.0	38.0
8	37.129341373036006	38.0	38.0	38.0	36.0	38.0
9	37.14292829346686	38.0	38.0	38.0	36.0	38.0
10-11	37.05740905529066	38.0	38.0	38.0	35.5	38.0
12-13	37.015213897706296	38.0	38.0	38.0	35.0	38.0
14-15	37.04075412056898	38.0	38.0	38.0	35.5	38.0
16-17	37.001866043324085	38.0	37.5	38.0	35.0	38.0
18-19	36.99077935531855	38.0	38.0	38.0	35.0	38.0
20-21	37.010309723347454	38.0	38.0	38.0	35.0	38.0
22-23	36.95760894107022	38.0	37.0	38.0	35.0	38.0
24-25	36.921818761372236	38.0	37.0	38.0	35.0	38.0
26-27	36.827842561725525	38.0	37.0	38.0	34.5	38.0
28-29	36.76454650498718	38.0	37.0	38.0	34.5	38.0
30-31	36.476870359794404	38.0	37.0	38.0	34.0	38.0
32-33	36.51667153653062	38.0	37.0	38.0	34.0	38.0
34-35	36.58813568326404	38.0	37.0	38.0	34.0	38.0
36-37	36.58635264898463	38.0	37.0	38.0	34.0	38.0
38-39	36.321152032725486	38.0	37.0	38.0	34.0	38.0
40-41	36.6475336352649	38.0	37.0	38.0	34.0	38.0
42-43	36.75696943939012	38.0	37.0	38.0	34.0	38.0
44-45	36.72646195529465	38.0	37.0	38.0	34.0	38.0
46-47	36.74586614957566	38.0	37.0	38.0	34.0	38.0
48-49	36.720485304078736	38.0	37.0	38.0	34.0	38.0
50-51	36.64252652969068	38.0	37.0	38.0	34.0	38.0
52-53	36.63405960713479	38.0	37.0	38.0	34.0	38.0
54-55	36.625941322566504	38.0	37.0	38.0	34.0	38.0
56-57	36.63794775078692	38.0	37.0	38.0	34.0	38.0
58-59	36.58036935704514	38.0	37.0	38.0	34.0	38.0
60-61	36.61106942212423	38.0	37.0	38.0	34.0	38.0
62-63	36.645059965733864	38.0	37.0	38.0	34.0	38.0
64-65	36.702425856321305	38.0	37.0	38.0	34.0	38.0
66-67	36.62531045382705	38.0	37.0	38.0	34.0	38.0
68-69	36.447305858446335	38.0	37.0	38.0	34.0	38.0
70-71	35.36195927908305	38.0	36.5	38.0	28.5	38.0
72-73	33.51023999574994	38.0	34.5	38.0	19.0	38.0
74-75	33.28970820660619	38.0	34.0	38.0	11.0	38.0
76	32.81621797511057	38.0	34.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	2.0
21	5.0
22	14.0
23	19.0
24	58.0
25	128.0
26	223.0
27	385.0
28	712.0
29	1068.0
30	1735.0
31	2732.0
32	3969.0
33	6256.0
34	10288.0
35	18792.0
36	24759.0
37	79440.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.874516887360045	15.675427994634294	11.170361122547913	37.27969399545775
2	18.67969133916832	16.480283691711048	41.06025792570359	23.77976704341705
3	17.629128869881665	18.304490457280227	38.26783366315594	25.798547009682178
4	13.045701459631044	26.455978643432985	32.03219422788307	28.4661256690529
5	12.984606802757229	30.160174252586565	42.434887705364375	14.420331239291833
6	26.473244524723416	43.19857091628704	16.027386344016044	14.300798214973504
7	25.450573094444373	26.072144820899684	18.219489195542746	30.2577928891132
8	22.116265788320295	42.25027559002829	15.910509609127011	19.722949012524403
9	20.86714568419375	16.199381084562976	16.652278432257976	46.2811947989853
10-11	22.644867384750242	27.771505983291938	27.81599883123265	21.76762780072517
12-13	22.061811855019723	18.804536942345237	29.037891968708912	30.095759233926128
14-15	20.40229503406691	19.288645690834475	20.149947538283772	40.15911173681484
16-17	28.43491426825867	27.9650830754519	19.695722045874117	23.90428061041531
18-19	28.62882339659729	22.64453534857158	20.431846253967834	28.294795000863292
20-21	20.921599617494323	21.24865525347642	20.498585525878898	37.33115960315036
22-23	21.291819956702483	28.5903071998725	20.17285803461145	29.945014808813568
24-25	28.354561513022457	28.91636672731861	20.278113503247315	22.450958256411617
26-27	28.599936249053698	29.88558033283307	20.722045874118443	20.792437543994794
28-29	28.295127037041958	22.762076155817937	21.105215624294424	27.837581182845682
30-31	28.917694872033255	28.397726216248522	20.770191120024435	21.91438779169378
32-33	21.706865180030015	28.89378826716959	21.007264951589125	28.392081601211267
34-35	27.186458236489447	22.454278618198238	21.519928811443297	28.839334333869022
36-37	22.027944164796196	27.378375147756103	28.794509449749643	21.799171237698058
38-39	19.857423664882525	22.29390514390448	30.15618981844262	27.692481372770377
40-41	19.902912621359224	19.83849760269879	30.766804351002087	29.491785424939902
42-43	27.340855059567293	19.930803660366834	30.152205384298675	22.5761358957672
44-45	19.94176085426268	27.33786673395933	31.208744504801246	21.511627906976745
46-47	26.62531709455062	20.124380752526797	24.91201041265456	28.33829174026802
48-49	26.044253781892074	19.593122866667553	23.69775410728753	30.664869244152843
50-51	19.69107353937285	20.100142111484466	31.152962426786026	29.05582192235666
52-53	17.54213539107221	20.27545721381802	38.862178422960966	23.320228972148804
54-55	17.23898635995378	20.261843730492874	32.95425869602752	29.544911213525825
56-57	24.64970183151156	20.08387233873003	25.342661336379212	29.923764493379203
58-59	18.35595606497284	20.34352463044373	37.42047733521044	23.880041969372982
60-61	24.6563425550848	28.034810672970927	24.866853492356526	22.441993279587745
62-63	17.70217682918731	37.79534618091988	21.573718672386544	22.928758317506276
64-65	17.44053232040163	38.81436521323363	20.542082265283625	23.20302020108111
66-67	17.28978789528907	38.89903443879245	19.88929913803408	23.9218785278844
68-69	17.32066725990464	38.98569588142324	19.875353618530276	23.818283240141845
70-71	18.126187029338716	37.385945572629595	20.105122654164397	24.382744743867292
72-73	19.100049141354443	33.32647125230765	20.894372650844037	26.67910695549387
74-75	19.632635171928335	31.784495238601195	21.090273996254634	27.492595593215835
76	19.694393901159472	31.799104830462326	20.66593175992456	27.84056950845364
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	2.5
15	2.5
16	2.0
17	4.0
18	15.5
19	25.5
20	44.0
21	64.0
22	74.0
23	107.0
24	186.0
25	242.0
26	302.0
27	412.0
28	506.0
29	550.0
30	706.5
31	1044.5
32	1381.0
33	1536.0
34	1864.0
35	2397.0
36	2957.5
37	3313.0
38	3627.0
39	4485.5
40	5535.0
41	9706.5
42	13373.0
43	14118.0
44	14230.0
45	12111.5
46	10626.0
47	9706.5
48	7958.0
49	6739.0
50	6349.0
51	5880.5
52	4963.0
53	4282.0
54	4050.0
55	3687.0
56	3064.5
57	2603.5
58	2402.0
59	2191.5
60	1896.0
61	1831.0
62	1851.0
63	1821.0
64	1787.5
65	1691.5
66	1648.0
67	1697.0
68	1646.5
69	1540.0
70	1431.0
71	1378.0
72	1288.0
73	1256.0
74	1234.5
75	1155.0
76	1051.5
77	899.5
78	754.5
79	658.0
80	595.5
81	450.5
82	314.0
83	260.0
84	220.0
85	141.0
86	80.5
87	59.0
88	49.5
89	27.5
90	8.5
91	2.5
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	150586.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.8352436481479
#Duplication Level	Percentage of deduplicated	Percentage of total
1	48.315763387155194	16.83091389637815
2	23.78519549345178	16.571261604664443
3	12.38347598985836	12.941442099531164
4	6.05829536572812	8.441687806303374
5	2.9757706311836363	5.183084748914242
6	1.6451569857216388	3.4385666662239514
7	1.084697943077187	2.645000199221707
8	0.8063747450292621	2.247220857184599
9	0.5452084564500448	1.7093222477521148
>10	2.3524029204872563	12.451356699826013
>50	0.020969556017309418	0.4236781639727465
>100	0.015250586194406847	0.9496234709733973
>500	0.005718969822902568	1.3606842601569868
>1k	0.0038126465486017116	2.5487097074097194
>5k	0.0	0.0
>10k+	0.0019063232743008558	12.25744757148739
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT	18458	12.25744757148739	No Hit
TCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG	2267	1.5054520340536304	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC	1571	1.0432576733560888	No Hit
ATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTCTT	882	0.5857118191598156	TruSeq Adapter, Index 2 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC	652	0.43297517697528326	No Hit
CTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTC	515	0.3419972640218878	No Hit
CTTTACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTC	297	0.19722949012524407	No Hit
CTTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG	213	0.1414474121100235	No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTACCTCGTATGCCGT	180	0.11953302431832971	No Hit
TTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTC	179	0.11886895196100568	No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC	164	0.10890786660114485	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0019922170719721623	0.0
2	0.0	0.0	0.0	0.0019922170719721623	0.0
3	0.0	0.0	0.0	0.0046485065012683785	0.0
4	0.0	0.0	0.0	0.012617374789157027	0.0
5	0.0	0.0	0.0	0.013945519503805135	0.0
6	0.0	0.0	0.0	0.013945519503805135	0.0
7	0.0	0.0	0.0	0.017265881290425406	0.0
8	0.0	0.0	0.0	0.018594026005073514	0.0
9	0.0	0.0	0.0	0.021914387791693784	0.0
10	0.0	0.0	0.0	0.022578460149017838	0.0
11	0.0	0.0	0.0	0.025898821935638108	0.0
12	0.0	0.0	0.0	0.02789103900761027	0.0
13	0.0	0.0	0.0	0.029219183722258377	0.0
14	0.0	0.0	0.0	0.030547328436906485	0.0
15	0.0	0.0	0.0	0.033203617866202705	0.0
16	0.0	0.0	0.0	0.033203617866202705	0.0
17	0.0	0.0	0.0	0.035195834938174866	0.0
18	0.0	0.0	0.0	0.036523979652822974	0.0
19	0.0	0.0	0.0	0.03718805201014703	0.0
20	0.0	0.0	0.0	0.04449284794071162	0.0
21	0.0	0.0	0.0	0.04714913737000784	0.0
22	0.0	0.0	0.0	0.05246171622860027	0.0
23	0.0	0.0	0.0	0.05711022272986865	0.0
24	0.0	0.0	0.0	0.06043058451648892	0.0
25	0.0	0.0	0.0	0.06308687394578513	0.0
26	0.0	0.0	0.0	0.0750401763776181	0.0
27	0.0	0.0	0.0	0.11687673488903351	0.0
28	0.0	0.0	0.0	0.1587132934004489	0.0
29	0.0	0.0	0.0	0.21117500962904917	0.0
30	0.0	0.0	0.0	0.27359781121751026	0.0
31	0.0	0.0	0.0	0.3493020599524524	0.0
32	0.0	0.0	0.0	0.4157092956848578	0.0
33	0.0	0.0	0.0	0.5186405110700862	0.0
34	0.0	0.0	0.0	0.6474705483909526	0.0
35	0.0	0.0	0.0	0.8221215783671789	0.0
36	0.0	0.0	0.0	1.0837660871528563	0.0
37	0.0	0.0	0.0	1.575179631572656	0.0
38	0.0	0.0	0.0	2.087843491426826	0.0
39	0.0	0.0	0.0	2.7167200138127052	0.0
40	0.0	0.0	0.0	3.3973941800698606	0.0
41	0.0	0.0	0.0	4.1989295153599935	0.0
42	0.0	0.0	0.0	5.131287105042966	0.0
43	0.0	0.0	0.0	6.098840529664113	0.0
44	0.0	0.0	0.0	7.03053404698976	0.0
45	0.0	0.0	0.0	7.889843677367086	0.0
46	0.0	0.0	0.0	8.76177068253357	0.0
47	0.0	0.0	0.0	9.557329366607785	0.0
48	0.0	0.0	0.0	10.360192846612566	0.0
49	0.0	0.0	0.0	11.1092664656741	0.0
50	0.0	0.0	0.0	11.819159815653514	0.0
51	0.0	0.0	0.0	12.595460401365333	0.0
52	0.0	0.0	0.0	13.54508387233873	0.0
53	0.0	0.0	0.0	14.388455766140279	0.0
54	0.0	0.0	0.0	15.33542294768438	0.0
55	0.0	0.0	0.0	16.158208598408883	0.0
56	0.0	0.0	0.0	16.80767136387181	0.0
57	0.0	0.0	0.0	17.46842335940924	0.0
58	0.0	0.0	0.0	18.08401843464864	0.0
59	0.0	0.0	0.0	18.625237405867743	0.0
60	0.0	0.0	0.0	19.263410941256158	0.0
61	0.0	0.0	0.0	19.948733614014582	0.0
62	0.0	0.0	0.0	20.5676490510406	0.0
63	0.0	0.0	0.0	21.226408829506063	0.0
64	0.0	0.0	0.0	21.93032552826956	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCGTCA	15	0.0022211405	70.0	2
TTGTACT	15	0.0022211405	70.0	4
GAGTTGC	15	0.0022211405	70.0	35
GTTATAG	15	0.0022211405	70.0	16
TTAATAC	25	2.3843459E-4	55.999996	3
CATGCCG	25	2.3843459E-4	55.999996	43
CGCATGC	25	2.3843459E-4	55.999996	41
GCCTATG	25	2.3843459E-4	55.999996	1
GGCCGAA	20	0.006940367	52.5	12
CGATTTC	20	0.006940367	52.5	16
GGCCCTA	20	0.006940367	52.5	12
GCACAAA	20	0.006940367	52.5	23
TAGATTG	20	0.006940367	52.5	64
AATACGG	20	0.006940367	52.5	6
CTTGAAA	2695	0.0	47.792206	57
TCTCGTA	2700	0.0	47.703705	38
CTCGTAT	2715	0.0	47.69797	39
TCGTATG	2725	0.0	47.651375	40
TCTTCTG	2675	0.0	47.626167	50
CCGTCTT	2675	0.0	47.626167	47
>>END_MODULE