FastQCFastQC Report
Wed 25 May 2016
SRR1779716_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779716_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80658
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTAGAT850.057.64348637
TCCGGAG252.3784133E-455.9965325
GTGTAGA1000.055.9965336
GTAGATC653.6379788E-1253.8428238
TTTAGAT200.00693043852.49674637
GTTTCGA200.00693043852.49674636
AGGCGTC200.00693043852.49674646
GTGGCTA200.00693043852.49674654
GACGATT200.00693043852.49674623
GCGTACC200.00693043852.49674636
TGCCGTA200.00693043852.49674653
GAGGCTC200.00693043852.49674629
CGTATCA552.242814E-950.90593356
GTATCAT552.242814E-950.90593357
TCCGACT352.0708505E-549.996920
CGCGTAC352.0708505E-549.996935
TACGCTG506.10853E-848.99696414
CCGACTA506.10853E-848.99696421
TAGATCT800.048.12201739
TATCATT604.8457878E-946.66377358
Produced by FastQC (version 0.11.5)