##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779716_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	80658
Sequences flagged as poor quality	0
Sequence length	76
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.7557588831858	32.0	23.0	34.0	12.0	34.0
2	26.767264251531156	32.0	21.0	34.0	12.0	34.0
3	25.82827493862977	31.0	21.0	34.0	11.0	34.0
4	27.63723375238662	32.0	23.0	34.0	12.0	34.0
5	27.244600659574996	32.0	23.0	34.0	11.0	34.0
6	29.31812095514394	34.0	22.0	38.0	11.0	38.0
7	30.315579359765927	36.0	26.0	38.0	12.0	38.0
8	29.53919016092638	35.0	22.0	38.0	11.0	38.0
9	31.094919288849216	37.0	26.0	38.0	12.0	38.0
10-11	30.533797019514495	36.0	26.0	38.0	11.0	38.0
12-13	29.889564581318652	35.5	23.5	38.0	11.0	38.0
14-15	29.58506285799301	34.5	23.0	38.0	11.0	38.0
16-17	29.41981576533016	35.0	23.5	38.0	11.0	38.0
18-19	29.944909370428228	34.5	24.0	38.0	11.0	38.0
20-21	28.84847132336532	33.5	22.5	37.5	11.0	38.0
22-23	28.92099977683553	33.5	23.5	37.5	11.0	38.0
24-25	29.849549951647695	35.0	24.0	38.0	11.0	38.0
26-27	28.521882516303403	33.5	21.5	38.0	11.0	38.0
28-29	30.323520295568947	36.5	24.5	38.0	11.0	38.0
30-31	30.794477919115277	37.0	26.0	38.0	11.0	38.0
32-33	30.930775620521214	37.0	26.0	38.0	11.0	38.0
34-35	30.99145775992462	37.0	26.0	38.0	11.0	38.0
36-37	29.199192888492153	34.5	23.0	38.0	11.0	38.0
38-39	27.80112326117682	32.0	21.0	37.0	11.0	38.0
40-41	28.59967393191004	34.0	21.5	37.0	11.0	38.0
42-43	29.536822137915642	35.0	23.5	37.5	11.0	38.0
44-45	31.026172233380446	37.0	26.0	38.0	11.0	38.0
46-47	29.777219866597235	35.5	24.0	38.0	11.0	38.0
48-49	29.05262962136428	35.0	22.5	38.0	11.0	38.0
50-51	27.196248357261524	31.0	20.0	37.0	11.0	38.0
52-53	30.06494086141486	35.0	24.0	37.5	11.0	38.0
54-55	29.271628356765603	35.0	23.0	38.0	11.0	38.0
56-57	29.65685362890228	35.5	23.5	38.0	11.0	38.0
58-59	30.641194921768452	36.5	25.0	38.0	11.0	38.0
60-61	31.15882491507352	37.0	26.0	38.0	11.0	38.0
62-63	31.067240695281313	37.0	26.0	38.0	11.0	38.0
64-65	30.765429343648492	37.0	25.5	38.0	11.0	38.0
66-67	29.00496540950681	34.0	21.5	38.0	11.0	38.0
68-69	28.973852562672022	34.0	21.5	38.0	11.0	38.0
70-71	28.57392323142156	34.0	21.5	38.0	11.0	38.0
72-73	27.53191251952689	32.0	21.0	37.0	11.0	38.0
74-75	29.1859146023953	35.0	22.5	37.5	11.0	38.0
76	29.444741997074065	35.0	23.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	2.0
5	1.0
6	0.0
7	0.0
8	1.0
9	0.0
10	2.0
11	8.0
12	36.0
13	224.0
14	582.0
15	1080.0
16	1624.0
17	2548.0
18	3263.0
19	3707.0
20	3115.0
21	2547.0
22	1977.0
23	1684.0
24	1501.0
25	1503.0
26	1585.0
27	1771.0
28	2028.0
29	2427.0
30	2775.0
31	3356.0
32	4348.0
33	5615.0
34	7326.0
35	9070.0
36	9902.0
37	5049.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.55134022663592	17.083240348136577	12.140147288551663	35.22527213667584
2	18.438344615537204	14.580078851446851	42.626893798507275	24.354682734508668
3	19.549207766123633	15.385950556671377	38.63224974584046	26.432591931364524
4	15.410746609139824	18.94542388851695	27.896798829626324	37.7470306727169
5	15.930223908353792	25.53373502938332	38.17228297255077	20.363758089712118
6	28.879962310000245	31.456272161471894	14.741253192491754	24.922512336036103
7	26.332167918867317	22.397034392124773	18.90327059932059	32.36752708968732
8	22.397182993813	31.871102128873076	17.53561553816969	28.196099339144236
9	21.803280721113907	14.07883153757455	17.973292995920797	46.14459474539075
10-11	25.59561079941725	21.49344409658721	25.842348346300486	27.06859675769505
12-13	24.016465599543725	16.52511375894263	28.832777454031465	30.625643187482176
14-15	25.44433354617535	20.301409096826585	21.749561400789787	32.50469595620827
16-17	27.502727768685215	19.1706343302088	23.7433665625155	29.58327133859049
18-19	23.642641935203898	18.49900189701561	26.419352038981813	31.439004128798683
20-21	27.359631014153134	18.3959778558897	24.54078248309125	29.703608646865913
22-23	28.176261267404808	17.772432519559096	24.21360643745428	29.83769977558181
24-25	24.398983324034468	22.007935031926102	20.766226520364516	32.82685512367492
26-27	26.419480624143727	21.186666584009572	24.197037983001568	28.196814808845133
28-29	25.22147209978488	21.70581561866503	22.819840428499695	30.252871853050394
30-31	24.69793248814358	21.22376863705403	23.913703852949382	30.164595021853014
32-33	25.222555607905374	20.695811739033402	23.90024053363751	30.181392119423712
34-35	24.467010111154508	21.309676579441685	24.058472353958603	30.164840955445204
36-37	27.05450577791003	17.782572037891185	28.815776422159402	26.347145762039382
38-39	26.181619798641076	16.794995784357486	32.46540693349204	24.5579774835094
40-41	25.290912134306243	21.721852662380737	23.133481708337726	29.853753494975294
42-43	22.018746745356708	22.13033451533712	25.132045527810153	30.718873211496025
44-45	22.032038485859175	19.07306609797528	27.932624948854972	30.962270467310578
46-47	25.530200177301666	18.339563689114545	27.53693268116077	28.59330345242302
48-49	25.95857588325367	18.720824266770816	28.000644733334575	27.319955116640937
50-51	29.29197121017687	17.360064969282178	28.570365824173784	24.777597996367177
52-53	21.147026732133114	20.810395278480385	27.724049000644747	30.318528988741754
54-55	24.81773545603333	19.265486286762883	25.94232009125626	29.974458165947524
56-57	25.0320812849872	19.5636945241741	25.15358721460055	30.250636976238155
58-59	21.577002715336068	21.452394827222793	26.6766270318525	30.29397542558863
60-61	21.677836122080805	20.219090779464736	26.338629783699	31.764443314755457
62-63	21.553225916442965	21.684655586070935	25.764554905984387	30.99756359150171
64-65	21.76745916121633	22.492173212237688	25.234183689284272	30.50618393726171
66-67	20.369118987247603	25.650483871967662	20.85268097927503	33.12771616150971
68-69	20.76453263059991	25.90482095799101	20.935668888544413	32.39497752286467
70-71	20.466817519605716	26.72328818077555	20.458758252998976	32.35113604661976
72-73	19.979541861690585	27.400266575741604	19.72908465329655	32.89110690927126
74-75	21.797216453302752	24.923591953132266	23.36505378010601	29.914137813458975
76	22.637041078225938	23.146644182961154	24.101375060445623	30.114939678367286
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.5
3	0.5
4	0.0
5	0.0
6	0.0
7	0.5
8	1.0
9	0.5
10	0.0
11	0.5
12	1.0
13	0.5
14	1.0
15	2.5
16	3.5
17	4.0
18	3.5
19	9.0
20	14.0
21	13.0
22	22.5
23	51.0
24	90.0
25	110.0
26	142.5
27	182.0
28	263.0
29	337.0
30	394.0
31	515.5
32	677.5
33	775.0
34	896.0
35	1182.5
36	1457.0
37	1566.0
38	1777.5
39	2092.0
40	2316.5
41	2579.5
42	2721.0
43	2824.0
44	3024.0
45	3177.5
46	3234.0
47	3187.5
48	3158.0
49	3085.5
50	2996.0
51	2953.0
52	2836.5
53	2670.5
54	2578.0
55	2627.0
56	2616.0
57	2583.5
58	2611.0
59	2639.5
60	2623.5
61	2556.5
62	2534.0
63	2483.5
64	2456.5
65	2439.0
66	2336.0
67	2274.0
68	2178.5
69	2035.5
70	1862.5
71	1737.0
72	1640.0
73	1403.5
74	1161.5
75	1059.0
76	954.5
77	751.5
78	559.0
79	465.0
80	401.0
81	291.0
82	200.0
83	155.0
84	120.5
85	64.5
86	35.0
87	27.0
88	17.5
89	9.0
90	8.0
91	4.0
92	2.0
93	2.0
94	3.0
95	2.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.006199013117111756
9	0.006199013117111756
10-11	0.006818914428822931
12-13	0.006199013117111756
14-15	0.004339309181978229
16-17	0.007438815740534108
18-19	0.006199013117111756
20-21	0.00557911180540058
22-23	0.006199013117111756
24-25	0.003719407870267054
26-27	0.004339309181978229
28-29	0.00557911180540058
30-31	0.006818914428822931
32-33	0.004959210493689405
34-35	0.00557911180540058
36-37	0.007438815740534108
38-39	0.007438815740534108
40-41	0.008058717052245283
42-43	0.004959210493689405
44-45	0.006199013117111756
46-47	0.00557911180540058
48-49	0.00557911180540058
50-51	0.00557911180540058
52-53	0.007438815740534108
54-55	0.007438815740534108
56-57	0.004339309181978229
58-59	0.006199013117111756
60-61	0.008058717052245283
62-63	0.008058717052245283
64-65	0.006818914428822931
66-67	0.008058717052245283
68-69	0.025415953780158197
70-71	0.006818914428822931
72-73	0.006818914428822931
74-75	0.006818914428822931
76	0.008678618363956458
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	80658.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	98.23204145899973
#Duplication Level	Percentage of deduplicated	Percentage of total
1	98.41604402261711	96.67608916660467
2	1.3908521809369951	2.732524982022862
3	0.17417205169628433	0.5132782860968534
4	0.01640751211631664	0.06446973641796226
5	0.0012621163166397415	0.006199013117111756
6	0.0012621163166397415	0.007438815740534108
7	0.0	0.0
8	0.0	0.0
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0037194078702670534	0.0
2	0.0	0.0	0.0	0.0037194078702670534	0.0
3	0.0	0.0	0.0	0.0037194078702670534	0.0
4	0.0	0.0	0.0	0.0037194078702670534	0.0
5	0.0	0.0	0.0	0.0037194078702670534	0.0
6	0.0	0.0	0.0	0.0037194078702670534	0.0
7	0.0	0.0	0.0	0.0037194078702670534	0.0
8	0.0	0.0	0.0	0.0037194078702670534	0.0
9	0.0	0.0	0.0	0.00991842098737881	0.0
10	0.0	0.0	0.0	0.01115822361080116	0.0
11	0.0	0.0	0.0	0.013637828857645863	0.0
12	0.0	0.0	0.0	0.016117434104490565	0.0
13	0.0	0.0	0.0	0.016117434104490565	0.0
14	0.0	0.0	0.0	0.01983684197475762	0.0
15	0.0	0.0	0.0	0.01983684197475762	0.0
16	0.0	0.0	0.0	0.01983684197475762	0.0
17	0.0	0.0	0.0	0.01983684197475762	0.0
18	0.0	0.0	0.0	0.01983684197475762	0.0
19	0.0	0.0	0.0	0.02231644722160232	0.0
20	0.0	0.0	0.0	0.02231644722160232	0.0
21	0.0	0.0	0.0	0.026035855091869373	0.0
22	0.0	0.0	0.0	0.035954276079248185	0.0
23	0.0	0.0	0.0	0.05207171018373875	0.0
24	0.0	0.0	0.0	0.06322993379453991	0.0
25	0.0	0.0	0.0	0.06818914428822931	0.0
26	0.0	0.0	0.0	0.07438815740534108	0.0
27	0.0	0.0	0.0	0.11902105184854571	0.0
28	0.0	0.0	0.0	0.16241414366832801	0.0
29	0.0	0.0	0.0	0.2008480249944209	0.0
30	0.0	0.0	0.0	0.2467207220610479	0.0012398026234223512
31	0.0	0.0	0.0	0.27275657715291723	0.0012398026234223512
32	0.0	0.0	0.0	0.3173894715961219	0.0012398026234223512
33	0.0	0.0	0.0	0.38433881326092884	0.0012398026234223512
34	0.0	0.0	0.0	0.46616578640680406	0.0012398026234223512
35	0.0	0.0	0.0	0.6496565746733121	0.0012398026234223512
36	0.0	0.0	0.0	0.7711572317687024	0.0012398026234223512
37	0.0	0.0	0.0	1.0724292692603337	0.0012398026234223512
38	0.0	0.0	0.0	1.3823799251159214	0.0012398026234223512
39	0.0	0.0	0.0	1.7518411068957822	0.0012398026234223512
40	0.0	0.0	0.0	2.1460983411440897	0.0012398026234223512
41	0.0	0.0	0.0	2.5837486672121797	0.0012398026234223512
42	0.0	0.0	0.0	3.047434848372139	0.0012398026234223512
43	0.0	0.0	0.0	3.4677279377123162	0.0012398026234223512
44	0.0	0.0	0.0	3.905378263780406	0.0012398026234223512
45	0.0	0.0	0.0	4.257482208832354	0.0012398026234223512
46	0.0	0.0	0.0	4.583550298792432	0.0024796052468447024
47	0.0	0.0	0.0	5.034838453718168	0.0024796052468447024
48	0.0	0.0	0.0	5.276599965285526	0.0024796052468447024
49	0.0	0.0	0.0	5.572912792283469	0.0024796052468447024
50	0.0	0.0	0.0	6.031639762949738	0.0024796052468447024
51	0.0	0.0	0.0	6.445733839172804	0.0024796052468447024
52	0.0	0.0	0.0	6.815195020952665	0.0024796052468447024
53	0.0	0.0	0.0	7.278881202112624	0.0024796052468447024
54	0.0	0.0	0.0	7.742567383272583	0.0024796052468447024
55	0.0	0.0	0.0	8.050038433881326	0.0024796052468447024
56	0.0	0.0	0.0	8.37362691859456	0.0024796052468447024
57	0.0	0.0	0.0	8.671179548215925	0.0024796052468447024
58	0.0	0.0	0.0	8.90054303354906	0.0024796052468447024
59	0.0	0.0	0.0	9.261325596964964	0.0024796052468447024
60	0.0	0.0	0.0	9.442336779984627	0.0024796052468447024
61	0.0	0.0	0.0	9.619628555134023	0.0024796052468447024
62	0.0	0.0	0.0	9.764685462074437	0.0024796052468447024
63	0.0	0.0	0.0	10.007686776265219	0.0024796052468447024
64	0.0	0.0	0.0	10.170100919933546	0.0024796052468447024
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGTAGAT	85	0.0	57.643486	37
TCCGGAG	25	2.3784133E-4	55.99653	25
GTGTAGA	100	0.0	55.99653	36
GTAGATC	65	3.6379788E-12	53.84282	38
TTTAGAT	20	0.006930438	52.496746	37
GTTTCGA	20	0.006930438	52.496746	36
AGGCGTC	20	0.006930438	52.496746	46
GTGGCTA	20	0.006930438	52.496746	54
GACGATT	20	0.006930438	52.496746	23
GCGTACC	20	0.006930438	52.496746	36
TGCCGTA	20	0.006930438	52.496746	53
GAGGCTC	20	0.006930438	52.496746	29
CGTATCA	55	2.242814E-9	50.905933	56
GTATCAT	55	2.242814E-9	50.905933	57
TCCGACT	35	2.0708505E-5	49.9969	20
CGCGTAC	35	2.0708505E-5	49.9969	35
TACGCTG	50	6.10853E-8	48.996964	14
CCGACTA	50	6.10853E-8	48.996964	21
TAGATCT	80	0.0	48.122017	39
TATCATT	60	4.8457878E-9	46.663773	58
>>END_MODULE