FastQCFastQC Report
Wed 25 May 2016
SRR1779716_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779716_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80658
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT1384217.161347913412182No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG17292.143618735897245No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC10701.3265888070619158No Hit
ATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTCTT7380.9149743360856952RNA PCR Primer, Index 47 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC5060.6273401274517098No Hit
CTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTC3550.44012993131493466No Hit
CTTTACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTC2610.32358848471323365No Hit
TTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTC1570.19464901187730912No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGACCTCGTATGCCGT1520.18844999876019736No Hit
CTTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG1450.17977138039624094No Hit
CTTAATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG1210.15001611743410448No Hit
TCTTAATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC1160.14381710431699274No Hit
TATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTCT1110.137618091199881No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATATCGTATGCCGT940.11654144660170102No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATTGG252.8170962E-670.0000127
GACATTG252.8170962E-670.0000126
GTCTGCG150.002217390670.07
CTGAGTG150.002217390670.09
AGACACG150.002217390670.012
GATTGTT150.002217390670.034
TTACCGG150.002217390670.019
TCTTTAT750.065.333331
CTATACA453.783498E-1062.2222181
AGACATT308.309949E-658.33333225
AACAATT200.00692873852.50000469
ATTACCG200.00692873852.50000418
AAAAGCC200.00692873852.50000470
AAGACAC200.00692873852.50000411
GTTAGGG200.00692873852.5000041
GTAGGCT200.00692873852.5000044
ATTCACA200.00692873852.50000436
GACCTTA200.00692873852.50000416
AGAGACA200.00692873852.5000046
CGTATGC22050.050.041
Produced by FastQC (version 0.11.5)