##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779716_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	80658
Sequences flagged as poor quality	0
Sequence length	76
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.567036127848446	34.0	34.0	34.0	34.0	34.0
2	33.59506806516403	34.0	34.0	34.0	34.0	34.0
3	33.61266086439039	34.0	34.0	34.0	34.0	34.0
4	33.66079000223164	34.0	34.0	34.0	34.0	34.0
5	33.65330159438617	34.0	34.0	34.0	34.0	34.0
6	37.24370800168613	38.0	38.0	38.0	36.0	38.0
7	37.24261697537752	38.0	38.0	38.0	36.0	38.0
8	37.110181259143545	38.0	38.0	38.0	36.0	38.0
9	37.12231892682685	38.0	38.0	38.0	36.0	38.0
10-11	37.02704629422996	38.0	38.0	38.0	35.0	38.0
12-13	36.990304743484835	38.0	38.0	38.0	35.0	38.0
14-15	37.02276897517915	38.0	38.0	38.0	35.0	38.0
16-17	36.941964839197595	38.0	37.5	38.0	35.0	38.0
18-19	36.935995189565816	38.0	38.0	38.0	35.0	38.0
20-21	36.99652855265442	38.0	38.0	38.0	35.0	38.0
22-23	36.92638052022118	38.0	37.0	38.0	35.0	38.0
24-25	36.896904212849314	38.0	37.0	38.0	34.5	38.0
26-27	36.79440972997099	38.0	37.0	38.0	34.5	38.0
28-29	36.64464157306157	38.0	37.0	38.0	34.0	38.0
30-31	36.396395893773715	38.0	37.0	38.0	34.0	38.0
32-33	36.46108259565077	38.0	37.0	38.0	34.0	38.0
34-35	36.471850281435195	38.0	37.0	38.0	34.0	38.0
36-37	36.55379503583029	38.0	37.0	38.0	34.0	38.0
38-39	35.89350095464802	38.0	37.0	38.0	32.0	38.0
40-41	36.4433782141883	38.0	37.0	38.0	34.0	38.0
42-43	36.72087703637581	38.0	37.0	38.0	34.0	38.0
44-45	36.67938084256986	38.0	37.0	38.0	34.0	38.0
46-47	36.70705943613777	38.0	37.0	38.0	34.0	38.0
48-49	36.671353120583206	38.0	37.0	38.0	34.0	38.0
50-51	36.56994966401349	38.0	37.0	38.0	34.0	38.0
52-53	36.53471447345582	38.0	37.0	38.0	34.0	38.0
54-55	36.52384760346153	38.0	37.0	38.0	34.0	38.0
56-57	36.57123905874185	38.0	37.0	38.0	34.0	38.0
58-59	36.4303354905899	38.0	37.0	38.0	34.0	38.0
60-61	36.4851471645714	38.0	37.0	38.0	34.0	38.0
62-63	36.51883260184979	38.0	37.0	38.0	34.0	38.0
64-65	36.56475489102135	38.0	37.0	38.0	34.0	38.0
66-67	36.46542190483275	38.0	37.0	38.0	34.0	38.0
68-69	36.2142440923405	38.0	37.0	38.0	34.0	38.0
70-71	34.62745170968782	38.0	35.5	38.0	26.0	38.0
72-73	32.01332167918867	38.0	27.0	38.0	11.0	38.0
74-75	31.681903840908525	38.0	25.0	38.0	11.0	38.0
76	31.280914478415035	37.0	25.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	4.0
21	4.0
22	8.0
23	26.0
24	27.0
25	83.0
26	145.0
27	228.0
28	410.0
29	708.0
30	1103.0
31	1570.0
32	2493.0
33	3724.0
34	6258.0
35	12206.0
36	14153.0
37	37506.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.50018597039352	13.837437080016862	11.261127228545217	42.40124972104441
2	16.61087554861266	13.90562622430509	45.21064246571946	24.27285576136279
3	17.787448238240472	15.253291675965187	41.363534925239904	25.595725160554437
4	13.740732475389919	29.471348161372706	27.63768008133105	29.15023928190632
5	14.651987403605347	27.209948176250343	42.85129807334672	15.28676634679759
6	27.64139948920132	42.638052022118075	13.476654516600957	16.243893972079647
7	24.359641945002355	23.336804780678914	15.685982791539587	36.61757048277914
8	21.29980907039599	43.84561977733145	14.125071288650847	20.72949986362171
9	20.192665327679833	14.043244315504971	13.433261424781175	52.330828932034024
10-11	24.119120236058418	27.291775149396212	25.738302462248008	22.85080215229735
12-13	22.423690148528355	14.947680329291577	28.210468893352182	34.41816062882789
14-15	21.416350516997696	15.110714374271616	16.786927521138633	46.68600758759205
16-17	32.99486721713903	26.188350814550326	14.829899080066452	25.98688288824419
18-19	33.54409977931513	18.46686007587592	15.509930819013611	32.479109325795335
20-21	22.91031267822163	16.41994594460562	15.500012398026234	45.16972897914652
22-23	22.7590567581641	26.926033375486625	15.297304669096679	35.0176051972526
24-25	33.340772149073864	27.07790919685586	15.142949242480597	24.438369411589676
26-27	33.15170224900196	29.1768950383099	15.475836246869498	22.195566465818644
28-29	23.329985866250095	28.935753428054255	16.238934761585956	31.495325944109698
30-31	22.724962186019987	28.788216915866993	25.972005256763126	22.514815641349898
32-33	41.96111978972947	17.643631133923478	19.18842520270773	21.206823873639316
34-35	25.566589798904015	25.916214138709115	27.173993900171094	21.343202162215775
36-37	31.936075776736345	27.58808797639416	18.21951945250316	22.256316794366338
38-39	22.1044409729971	18.295767313843637	27.830469389273226	31.76932232388604
40-41	20.953284237149447	15.604775719705422	29.538297503037512	33.90364254010761
42-43	31.282699794192766	15.431203352426293	28.99340425004339	24.29269260333755
44-45	21.213022886756427	25.95092861216494	30.250564110193658	22.58548439088497
46-47	30.812194698603985	16.556324233182078	21.090282427037614	31.541198641176326
48-49	30.213989932802697	15.554563713456817	18.937985072776414	35.293461280964074
50-51	21.337003149098663	15.839098415532247	29.731706712291405	33.09219172307768
52-53	19.08490168365196	16.00337226313571	40.40578739864614	24.505938654566194
54-55	19.02539115772769	15.719457462371992	31.962731533139927	33.292419846760396
56-57	29.22896674849364	15.927744303106945	21.584343772471424	33.25894517592799
58-59	20.36499789233554	16.84767784968633	37.99685090133651	24.790473356641623
60-61	29.499863621711427	27.369882714671824	20.530511542562422	22.599742121054327
62-63	19.170448064668104	41.10069676907436	16.751593146371096	22.977262019886435
64-65	18.35651764239133	42.756453172654915	16.0194896972402	22.867539487713557
66-67	18.7321778372883	42.570482779141564	15.844057626025936	22.8532817575442
68-69	18.57224329886682	42.6058171539091	16.231495945845424	22.59044360137866
70-71	18.789828659277443	40.76099085025664	16.59971732500186	23.84946316546406
72-73	19.33534181358328	35.18807805797317	18.615636390686603	26.860943737756948
74-75	20.319745096580625	32.30367725458107	19.859778323290932	27.516799325547375
76	20.336482431996828	33.041979716829076	19.6583103969848	26.963227454189294
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.5
15	1.5
16	6.0
17	10.0
18	9.5
19	13.0
20	26.5
21	36.0
22	44.5
23	57.0
24	81.0
25	101.0
26	120.5
27	180.5
28	255.5
29	290.0
30	328.5
31	418.0
32	543.0
33	617.0
34	675.0
35	805.5
36	984.0
37	1090.0
38	1319.0
39	4239.5
40	7452.0
41	7122.0
42	6271.0
43	5357.5
44	3756.0
45	2644.0
46	2220.0
47	2040.0
48	1782.0
49	1749.0
50	1794.0
51	1692.0
52	1459.5
53	1421.5
54	1514.0
55	1521.5
56	1569.0
57	1706.0
58	1803.0
59	1792.0
60	1852.5
61	1942.5
62	1961.0
63	1908.5
64	1962.0
65	2024.5
66	2087.5
67	2194.0
68	2163.5
69	2038.0
70	1885.0
71	1827.0
72	1748.0
73	1588.5
74	1428.0
75	1348.0
76	1256.5
77	1009.0
78	812.5
79	772.0
80	656.0
81	440.5
82	271.5
83	202.0
84	161.5
85	110.0
86	70.5
87	42.0
88	33.5
89	19.0
90	9.0
91	5.0
92	5.0
93	2.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	80658.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.416648069627314
#Duplication Level	Percentage of deduplicated	Percentage of total
1	57.46880264999	24.951027796374817
2	24.518118735543563	21.289890649408612
3	10.922641994345925	14.226735103771478
4	4.197721237042748	7.290039425723426
5	1.5762871583997258	3.4218552406456895
6	0.6110968331477198	1.5919065684742988
7	0.2741368971129958	0.83314736293982
8	0.13135726319997715	0.4562473654194253
9	0.05140066820868672	0.2008480249944209
>10	0.19703589479996572	1.4071759775843686
>50	0.014277963391301864	0.39921644474199713
>100	0.022844741426082983	1.7580401200128941
>500	0.005711185356520746	1.5423144635374049
>1k	0.005711185356520746	3.470207542959161
>5k	0.0	0.0
>10k+	0.002855592678260373	17.161347913412182
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT	13842	17.161347913412182	No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG	1729	2.143618735897245	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC	1070	1.3265888070619158	No Hit
ATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTCTT	738	0.9149743360856952	RNA PCR Primer, Index 47 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC	506	0.6273401274517098	No Hit
CTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTC	355	0.44012993131493466	No Hit
CTTTACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTC	261	0.32358848471323365	No Hit
TTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTC	157	0.19464901187730912	No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGACCTCGTATGCCGT	152	0.18844999876019736	No Hit
CTTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG	145	0.17977138039624094	No Hit
CTTAATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG	121	0.15001611743410448	No Hit
TCTTAATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC	116	0.14381710431699274	No Hit
TATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTCT	111	0.137618091199881	No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATATCGTATGCCGT	94	0.11654144660170102	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.004959210493689405	0.0
2	0.0	0.0	0.0	0.004959210493689405	0.0
3	0.0	0.0	0.0	0.004959210493689405	0.0
4	0.0	0.0	0.0	0.004959210493689405	0.0
5	0.0	0.0	0.0	0.004959210493689405	0.0
6	0.0	0.0	0.0	0.004959210493689405	0.0
7	0.0	0.0	0.0	0.007438815740534107	0.0
8	0.0	0.0	0.0	0.007438815740534107	0.0
9	0.0	0.0	0.0	0.014877631481068214	0.0
10	0.0	0.0	0.0	0.017357236727912916	0.0
11	0.0	0.0	0.0	0.02107664459817997	0.0
12	0.0	0.0	0.0	0.023556249845024673	0.0
13	0.0	0.0	0.0	0.023556249845024673	0.0
14	0.0	0.0	0.0	0.026035855091869373	0.0
15	0.0	0.0	0.0	0.026035855091869373	0.0
16	0.0	0.0	0.0	0.026035855091869373	0.0
17	0.0	0.0	0.0	0.027275657715291727	0.0
18	0.0	0.0	0.0	0.027275657715291727	0.0
19	0.0	0.0	0.0	0.028515460338714077	0.0
20	0.0	0.0	0.0	0.03099506558555878	0.0
21	0.0	0.0	0.0	0.03719407870267054	0.0
22	0.0	0.0	0.0	0.04339309181978229	0.0
23	0.0	0.0	0.0	0.0533115128071611	0.0
24	0.0	0.0	0.0	0.06075032854769521	0.0
25	0.0	0.0	0.0	0.06818914428822931	0.0
26	0.0	0.0	0.0	0.07686776265218577	0.0
27	0.0	0.0	0.0	0.13389868332961394	0.0
28	0.0	0.0	0.0	0.19836841974757619	0.0
29	0.0	0.0	0.0	0.27275657715291723	0.0
30	0.0	0.0	0.0	0.35086414242852537	0.0
31	0.0	0.0	0.0	0.41781348409333235	0.0
32	0.0	0.0	0.0	0.5008802598626299	0.0
33	0.0	0.0	0.0	0.6335391405688214	0.0
34	0.0	0.0	0.0	0.8467851917974658	0.0
35	0.0	0.0	0.0	1.150536834535942	0.0
36	0.0	0.0	0.0	1.5051203848347343	0.0
37	0.0	0.0	0.0	2.025837486672122	0.0
38	0.0	0.0	0.0	2.6011059039400926	0.0
39	0.0	0.0	0.0	3.2073693867936224	0.0
40	0.0	0.0	0.0	3.8173522775174193	0.0
41	0.0	0.0	0.0	4.444692404969129	0.0
42	0.0	0.0	0.0	5.086910163901907	0.0
43	0.0	0.0	0.0	5.690694041508592	0.0
44	0.0	0.0	0.0	6.278360485010786	0.0
45	0.0	0.0	0.0	7.019762453817353	0.0
46	0.0	0.0	0.0	7.628505541917726	0.0
47	0.0	0.0	0.0	8.239728235264947	0.0
48	0.0	0.0	0.0	8.756725929232067	0.0
49	0.0	0.0	0.0	9.335713754370305	0.0
50	0.0	0.0	0.0	9.923380197872499	0.0
51	0.0	0.0	0.0	10.419301247241439	0.0
52	0.0	0.0	0.0	10.94745716481936	0.0
53	0.0	0.0	0.0	11.473133477150437	0.0
54	0.0	0.0	0.0	12.018646631456273	0.0
55	0.0	0.0	0.0	12.510848272954945	0.0
56	0.0	0.0	0.0	12.989412085595973	0.0
57	0.0	0.0	0.0	13.461776885119889	0.0
58	0.0	0.0	0.0	13.88454957970691	0.0
59	0.0	0.0	0.0	14.423863720895634	0.0
60	0.0	0.0	0.0	14.862753849587145	0.0
61	0.0	0.0	0.0	15.30288378090208	0.0
62	0.0	0.0	0.0	15.68846239678643	0.0
63	0.0	0.0	0.0	16.18810285402564	0.0
64	0.0	0.0	0.0	16.593518311884747	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACATTGG	25	2.8170962E-6	70.00001	27
GACATTG	25	2.8170962E-6	70.00001	26
GTCTGCG	15	0.0022173906	70.0	7
CTGAGTG	15	0.0022173906	70.0	9
AGACACG	15	0.0022173906	70.0	12
GATTGTT	15	0.0022173906	70.0	34
TTACCGG	15	0.0022173906	70.0	19
TCTTTAT	75	0.0	65.33333	1
CTATACA	45	3.783498E-10	62.222218	1
AGACATT	30	8.309949E-6	58.333332	25
AACAATT	20	0.006928738	52.500004	69
ATTACCG	20	0.006928738	52.500004	18
AAAAGCC	20	0.006928738	52.500004	70
AAGACAC	20	0.006928738	52.500004	11
GTTAGGG	20	0.006928738	52.500004	1
GTAGGCT	20	0.006928738	52.500004	4
ATTCACA	20	0.006928738	52.500004	36
GACCTTA	20	0.006928738	52.500004	16
AGAGACA	20	0.006928738	52.500004	6
CGTATGC	2205	0.0	50.0	41
>>END_MODULE